Volume 6, Issue 1 (3-2016)                   J Adv Biomed Sci. 2016, 6(1): 120-128 | Back to browse issues page

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Moradi M, Norouzi A, Taati moghadam M. Prevalence of bla-CTX-M, bla-SHV, and bla-TEM Genes and Comparison of Antibiotic Resistance Pattern in Extended-spectrum β-lactamase producing and non-producing groups of Klebsiella pneumoniae Isolated from Clinical Samples in Kerman Hospitals. J Adv Biomed Sci. 2016; 6 (1) :120-128
URL: http://jabs.fums.ac.ir/article-1-862-en.html
Prevalence of bla-CTX-M, bla-SHV, and bla-TEM Genes and Comparison of Antibiotic Resistance Pattern in Extended-spectrum β-lactamase producing and non-producing groups of Klebsiella pneumoniae Isolated from Clinical Samples in Kerman Hospitals
Mohammad Moradi1, Amin Norouzi2, Majid Taati moghadam *3
1- Department of Microbiology, Kerman University of Medical Sciences, Kerman, Iran
2- Students Research Committee, Department of Microbiology, Kerman University of Medical Sciences, Kerman, Iran
3- Students Research Committee, Department of Microbiology, Kerman University of Medical Sciences, Kerman, Iran , majidtaati1367@yahoo.com
Abstract:   (11573 Views)

Background & Objectives: Antibiotic resistance among pathogens bacteria are an important problem noted worldwide. Beta-lactamases that are produced by Enterobacteriaceae have been located mainly on plasmid. Treatment of these bacterial infections which produced β-lactamase are a major problem.
Materials & Methods: 111 Klebsiella pneumoniae isolates were collected from hospitals in Kerman; therefore, antibiotic susceptibility test was performed by disk diffusion method. At first, detection of ESBLs were performed by phenotypic confirmatory test, and presence of bla-SHV, bla-TEM, and bla-CTX-M were detected by PCR.
Results: Resistant to ampicillin (92.5%) was more than others antibiotics, and the imipenem (89%) was the most effective antibiotic against Klebsiella pneumoniae isolates. Additionally, the resistance to all antibiotics in ESBLs-producing Klebsiella pneumoniae was more than that of Non-ESBL Klebsiella pneumoniae. After the detection of bla-SHV, bla-TEM, and bla-CTX-M genes in Klebsiella pneumoniae by PCR, 56 (50.4%) isolates presented these genes.
Conclusion: With regard to high prevalence of ESBLs genes and high level of antibiotic resistance in bacteria, detecting these genes can prevent the extension of antibiotic resistance through these bacteria.

Keywords: Klebsiella pneumonia, Antibiotic resistance, β- lactamase genes, PCR
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Type of Study: Research | Subject: Microbiology
Received: 2015/04/8 | Revised: 2016/06/9 | Accepted: 2015/11/21 | Published: 2016/06/17
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