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:: Volume 10, Issue 2 (Summer 2020) ::
JABS 2020, 10(2): 2348-2354 Back to browse issues page
IMolecular Analysis of AmpC Genomic Polymorphism in Shigella Sonnei Isolated from CLinical Specimens by PCR-RFLP Method
Abolfazl Abasi1 , Kumarss Amini 2
1- Department of Microbiolog, Faculty of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran
2- Department of Microbiolog, Faculty of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran , dr_kumarss_amini@yahoo.com
Abstract:   (1491 Views)
Background & Objective: Shigella is a gram-negative bacillus that has caused serious infection problems in developed and developing countries. The existence of beta-lactamase genes such as AmpC in Shigella species in one country has led to a variety of antibiotic resistance patterns, which can have very serious health risks for the community. The aim of this study was to investigate the prevalence of AmpC β-lactamase gene in S. sonnei strains and determine the prevalence of a spot mutation in this gene by RFLP method.
Materials & methods: Sixty strains of S. sonei were detected using biochemical and microbiological tests. Subsequently, the amplification of ampC gene was performed by PCR method. Then, a point mutation analysis on this gene was performed on ampC gene positive strains. RFLP technique was performed using Hinf1 enzyme.
Results: Among 60 isolates, 30 (50%) isolates were positive for ampC beta-lactamase gene. Furthermore, A to G point mutation was detected in 11 (37%) ampC gene carrying isolates.
Conclusion: The results of this study showed an increasing rate in the prevalence of ampC gene and noticeable prevalence of A to G point mutation in the strains carrying this gene. This mutation may help increase resistance to cephalosporins.
 
Keywords: Shigella sonnei, ampC beta-lactamase gene, RFLP-PCRalization time, medical costs, and in particular reducing mortality rates
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Type of Study: Research | Subject: Microbiology
Received: 2018/07/4 | Accepted: 2020/01/13 | Published: 2020/09/19
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Abasi A, Amini K. IMolecular Analysis of AmpC Genomic Polymorphism in Shigella Sonnei Isolated from CLinical Specimens by PCR-RFLP Method. JABS. 2020; 10 (2) :2348-2354
URL: http://jabs.fums.ac.ir/article-1-1774-en.html


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Volume 10, Issue 2 (Summer 2020) Back to browse issues page
Journal of Fasa University of Medical Sciences
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