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:: Volume 4, Issue 4 (Winter 2015) ::
JABS 2015, 4(4): 402-408 Back to browse issues page
The effect of aqueous extract of Phoenix Dactylifera Pollen on In vitro viability and proliferation rate of neonatal mouse spermatogonial stem cells
Maryam Mahaldashtian1, Majid Naghdi2, Mohammad Taghi Ghorbanian1, Morteza Koruji3, Zohre Makoolati 4, Mohammad Mehdi Naghizadeh5, Seyed Amin Kouhpayeh6, Abbas Abdollahi7, Mohammad Ebrahim Astaneh2
1- Department of Molecular and Cellular Biology, Faculty of Biology, Damghan University, Semnan, Iran.
2- Department of Anatomical Sciences, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran.
3- Cellular and Molecular Research Center and Department of Anatomical Sciences, School of Medicine, Iran University of Medical Science, Tehran, Iran.
4- Department of Anatomical Sciences, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran. , zohreh1438@yahoo.com
5- Department of Community Medicine, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran.
6- Department of Pharmacology, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran
7- Department of Microbiology, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran.
Abstract:   (14696 Views)
Introduction: There is a fast growing tendency in the consumption of herbal remedies in the developing countries. One of the traditional medicines used for male infertility is Date palm (Phoenix dactylifera) pollen (DPP). The goal of the present study was to investigate the effect of aqueous extract of DPP on In vitro viability and proliferation rate of neonate mouse spermatogonial stem cells (SSCs). Methods: cell suspension includes sertoli cells and SSCs were isolated from neonatal 6 day-old mice testes by 2 steps enzymatic digestion. The cell suspension was cultured in DMEM and FCS 4% in the absence or presence of 0.06, 0.25 and 0.62 mg/ml of aqueous extract of DPP for 2 weeks. In order to evaluate the rate of SSCs expansion at the end of culture, the mean number of whole cells and living cells were considered as proliferation and survival rates respectively. Data analysis was done with ANOVA test. The significancy of the data was analyzed using ANOVA and Tukey post test. Results: The results showed that there were no significant differences between the mean percent of viability and proliferation rate between control and 0.06, 0.25 and 0.62 mg/ml of DPP-treated groups (P> 0.05). Conclusion: Our study showed that treatment of neonatal mouse testicular cell suspension with DPP had no toxic effects on viability percent and proliferation rate of these cells. Thus, we can use DPP for evaluate the in vitro pattern of SSCs colonization in the future studies.
Keywords: Spermatogonial Stem Cell, Date Palm Pollen, viability, proliferation
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Type of Study: Research | Subject: Embryology
Received: 2014/04/17 | Accepted: 2014/08/6 | Published: 2015/02/2
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Mahaldashtian M, Naghdi M, Ghorbanian M T, Koruji M, Makoolati Z, Naghizadeh M M, et al . The effect of aqueous extract of Phoenix Dactylifera Pollen on In vitro viability and proliferation rate of neonatal mouse spermatogonial stem cells. JABS. 2015; 4 (4) :402-408
URL: http://jabs.fums.ac.ir/article-1-461-en.html


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Volume 4, Issue 4 (Winter 2015) Back to browse issues page
Journal of Fasa University of Medical Sciences
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