Journal of Advanced Biomedical Sciences
JABS
Sat, Feb 22, 2025
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Volume 14, Issue 4 (9-2024)
JABS 2024, 14(4): 310-318 |
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Ghanbariasad A, Ghasemian A, Mansoori Y, Montaseri Z, Alinejad N, Doustan M et al . Detection of Coronavirus Disease 2019 (COVID-19) Virus on the Surface of Hospital Settings by Quantitative Real-Time Polymerase Chain Reaction. JABS 2024; 14 (4) :310-318
URL: http://jabs.fums.ac.ir/article-1-3089-en.html
URL: http://jabs.fums.ac.ir/article-1-3089-en.html
Ali Ghanbariasad1 
, Abdolmajid Ghasemian2 , Yaser Mansoori3 , Zahra Montaseri2 , Navid Alinejad * 4, Mohammad Doustan5 , Ramin Hayati6
, Abdolmajid Ghasemian2 , Yaser Mansoori3 , Zahra Montaseri2 , Navid Alinejad * 4, Mohammad Doustan5 , Ramin Hayati6
1- Department of Medical Biotechnology, Fasa University of Medical Sciences, Fasa, Iran
2- Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran
3- Department of Medical Genetic, Fasa University of Medical Sciences, Fasa, Iran
4- Department of Public Health, School of Health, Fasa University of Medical Sciences, Fasa, Iran ,navidalinejad@yahoo.com
5- Student Research Committee, Fasa University of Medical Sciences, Fasa, Iran
6- Department of Public Health, School of Health, Fasa University of Medical Sciences, Fasa, Iran
2- Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran
3- Department of Medical Genetic, Fasa University of Medical Sciences, Fasa, Iran
4- Department of Public Health, School of Health, Fasa University of Medical Sciences, Fasa, Iran ,
5- Student Research Committee, Fasa University of Medical Sciences, Fasa, Iran
6- Department of Public Health, School of Health, Fasa University of Medical Sciences, Fasa, Iran
Abstract: (300 Views)
Background & Objectives: Coronavirus disease 2019 (COVID-19) has had a profound impact on human health, with over 704,753,890 confirmed cases and 7,010,681 deaths reported to date. This study aimed to evaluate the contamination rate of COVID-19 on high-risk surfaces at Valiasr Hospital in Fasa, Iran, using molecular testing.
Materials & Methods: A total of 142 surface swabs, immersed in viral transport medium (VTM), were collected and transported to the Virology Reference Laboratory at the Cohort Center of Fasa University of Medical Sciences for COVID-19 virus testing. The presence of the virus was assessed using the real-time polymerase chain reaction (RT-qPCR) technique (QIAquant 96 5plex), following the manufacturer’s protocol (Qiagen, MD, USA).
Results: Among the 142 samples obtained from surfaces and equipment in the COVID-19 ward, two samples tested positive for COVID-19. Similarly, two samples from the coronavirus isolation ward were found to be positive using RT-qPCR. The positive samples were collected from a patient’s bed and the interior bed of an insulated room. No COVID-19 contamination was detected on hospital surface samples outside these areas.
Conclusion: This study identified a low rate of COVID-19 contamination on hospital surfaces and equipment in Fasa city. The findings suggest that the hospital environment could serve as a potential source of COVID-19 transmission, particularly among healthcare providers, visitors, and patients.
Materials & Methods: A total of 142 surface swabs, immersed in viral transport medium (VTM), were collected and transported to the Virology Reference Laboratory at the Cohort Center of Fasa University of Medical Sciences for COVID-19 virus testing. The presence of the virus was assessed using the real-time polymerase chain reaction (RT-qPCR) technique (QIAquant 96 5plex), following the manufacturer’s protocol (Qiagen, MD, USA).
Results: Among the 142 samples obtained from surfaces and equipment in the COVID-19 ward, two samples tested positive for COVID-19. Similarly, two samples from the coronavirus isolation ward were found to be positive using RT-qPCR. The positive samples were collected from a patient’s bed and the interior bed of an insulated room. No COVID-19 contamination was detected on hospital surface samples outside these areas.
Conclusion: This study identified a low rate of COVID-19 contamination on hospital surfaces and equipment in Fasa city. The findings suggest that the hospital environment could serve as a potential source of COVID-19 transmission, particularly among healthcare providers, visitors, and patients.
Type of Study: Research |
Subject:
Microbiology
Received: 2024/07/24 | Accepted: 2024/11/23 | Published: 2024/12/21
Received: 2024/07/24 | Accepted: 2024/11/23 | Published: 2024/12/21
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This work is licensed under a Creative Commons — Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)