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:: Volume 10, Issue 3 (Atumn 2020) ::
J Fasa Univ Med Sci 2020, 10(3): 2545-2557 Back to browse issues page
 The Effect of Ethephon Investigation on Sperm Parameters and in Vitro Fertilizing Potential in Adult Male Mice
Ramin Jahangirfard1 , Gholamreza Najafi 2, Ali Shalizar Jalali3 , Abbas Ahmadi4 , Elham Zadeh hashem5
1- Department of Comparative Anatomy and Embryology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
2- Department of Veterinary Anatomical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran , G.najafi2006@yahoo.com
3- Department of Comparative Histology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
4- Department of Veterinary Anatomical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
5- Department of Toxicology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Abstract:   (918 Views)
Background & Objectives: Ethephon is one of the most important organophosphorus pesticides that has an adverse effect on the reproductive system and can impair the semen quality and decline the fertility. The aim of this study was to evaluate the effect of ethephon on sperm parameters, characteristics and in vitro fertilizing potential in male mice.
Materials & Methods: In this experimental study, 90 adult male mice were divided into 6 control and experimental groups. The experimental groups received ethephon at low, medium and high doses and the positive control group received neostigmine bromide (0.1 mg/kg). After treatment, animals were euthanized by cervical dislocation and sperm samples were collected from cauda epididymis to evaluate sperm parameters and in vitro fertilization. The level of p<0.05 was considered significant.
Results: A decrease in sperm motility and viability was observed between the experimental groups compared to the control group. Nonetheless, ethephon can remarkably increase DNA damage and lack of nuclear maturation. In vitro fertilization results showed that ethephon significantly reduced embryonic quality and stages of embryological development compared to the control group. Also, the percentage of arrested embryos in the experimental groups was significantly enhanced compared to the control group (p<0.05).
Conclusion: The present results showed that oxidative stress induced by ethephon causes damage to sperm DNA and subsequently decreases the quality of sperm and in vitro fertilizing potential in a dose dependent manner.

Keywords: Ethephon, IVF, Embryonic development, Sperm, Mouse
Full-Text [PDF 1384 kb]   (136 Downloads)    
Type of Study: Research | Subject: Embryology
Received: 2020/02/1 | Accepted: 2020/05/2 | Published: 2021/01/26
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Jahangirfard R, Najafi G, Shalizar Jalali A, Ahmadi A, zadeh hashem E.  The Effect of Ethephon Investigation on Sperm Parameters and in Vitro Fertilizing Potential in Adult Male Mice. J Fasa Univ Med Sci. 2020; 10 (3) :2545-2557
URL: http://jabs.fums.ac.ir/article-1-2242-en.html


Volume 10, Issue 3 (Atumn 2020) Back to browse issues page
Journal of Fasa University of Medical Sciences
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