Volume 9, Issue 2 (7-2019)                   JABS 2019, 9(2): 1443-1456 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Sadeghpour H, Sabet E, Dehshahri A. Preparation of Hydrocortisone Conjugated Polyethylenimine Nanocarriers and its Characterizations for Potential Application in Targeted Gene Delivery . JABS 2019; 9 (2) :1443-1456
URL: http://jabs.fums.ac.ir/article-1-1894-en.html
1- Department of Pharmaceutical Chemistry, Faculty of pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
2- . Department of Pharmaceutical Chemistry, Faculty of pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
3- Department of Pharmaceutical Biotechnology, Faculty of pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran , dehshahria@sums.ac.ir
Abstract:   (3347 Views)
Background & objective: nowadays, gene therapy has been considered as a novel therapeutic approach. Particularly, following the introduction of immunotherapy and gene editing using CRISPR/Cas9 platform great attention has been directed to gene therapy. However, finding an efficient carrier with low toxicity is still one of the major challenges for researchers. Among non-viral gene carriers, polyethyleneimine (PEI) could be considered as the most studied polycationic compound for gene delivery. However, toxicity, lack of targeting and non-specific interactions with serum components limit its wide application. Therefore, PEI structure must be modified through chemical conjugations. In this study, conjugation of hydrocortisone on the polymer structure was carried out in order to direct the carriers into the cell nucleus.
Materials & Methods: hydrocortisone was conjugated onto polyethylenimine followed by the grafting of the targeted PEI domain onto another unmodified polymer via succinic acid linker. The conjugate w::as char::acterized in terms of buffering capacity, zeta potential, particle size, protection of DNA against enzymes and condensation ability.
Results: The results demonstrated that the conjugates could condense plasmid DNA successfully and form nanoparticles with the size of around 300 nm. Meanwhile, the conjugates showed higher buffering capacity compared with the unmodified polymer. Also, the protection ability of the conjugates was significant rather than the parent polymer.
Conclusion: According to the results, design and synthesis of the PEI derivative with two separated domains responsible for targeting and plasmid condensation could be considered as an efficient strategy to create nano gene delivery systems.
 
 
Full-Text [PDF 874 kb]   (1020 Downloads)    
Type of Study: Research | Subject: Nanomedicine
Received: 2019/01/1 | Accepted: 2019/05/4 | Published: 2019/09/18

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Advanced Biomedical Sciences

Designed & Developed by: Yektaweb

Creative Commons License
This work is licensed under a Creative Commons — Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)