fa ارزیابی اثرات ضد باکتریایی عصاره‌های گلسنگ SP. Lecanora muralis بر استافیلوکوکوس اورئوس و سالمونلا تیفی موریوم در شرایط آزمایشگاهی و مدل حیوانی ميكروبیولوژی Microbiology پژوهشي Research <p style="direction: rtl"><font face="Times New Roman" size="3"> </font><b><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt mso-bidi-language: FA">زمینه و هدف</span></b><span dir="ltr"></span><span dir="ltr"></span><b><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>:</span></b><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr"> </span><i><span lang="AR-SA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">استافیلوکوکوس</span></i><span dir="ltr"></span><span dir="ltr"></span><b><i><span lang="AR-SA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span></i></b><i><span lang="AR-SA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">اورئوس</span></i><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt mso-bidi-language: FA">و</span><span dir="ltr"></span><span dir="ltr"></span><span lang="FA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><i><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt mso-bidi-language: FA">سالمونلا تیفی موریوم</span></i><span dir="ltr"></span><span dir="ltr"></span><span lang="FA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">از</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">عوامل</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">مهم</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">عفونی</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">و</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt mso-bidi-language: FA">امروزه</span><span dir="ltr"></span><span dir="ltr"></span><span lang="FA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">مقاوم به آنتی بیوتیک‌ها بوده و یافتن ترکیبات ضد میکروبی علیه آن‌ها ضروری می‌باشد. هدف از این</span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt mso-bidi-language: FA">پژوهش ارزیابی فعالیت ضد باکتریایی عصاره‌های</span><span dir="ltr"></span><span dir="ltr"></span><span lang="FA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><i><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr">SP. Lecanora muralis</span></i><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"> </span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt mso-bidi-language: FA">علیه این باکتری‌ها در شرایط آزمایشگاهی و مدل حیوانی است</span><span dir="ltr"></span><span dir="ltr"></span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>.</span></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt text-align: justify line-height: normal unicode-bidi: embed direction: rtl" dir="rtl"><b><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt mso-bidi-language: FA">مواد و روش‌ها</span></b><span dir="ltr"></span><span dir="ltr"></span><b><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>:</span></b><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"> </span><span lang="AR-SA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">در این مطالعه عصاره‌های آبی، استونی و اتانولی</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><i><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr">SP. Lecanora muralis</span></i><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"> </span><span lang="AR-SA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">تهیه و اثر ضد میکروبی آن‌ها با روش انتشار چاهکی در آگار و میزان</span><span dir="ltr"></span><span dir="ltr"></span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> MIC </span><span lang="AR-SA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">و</span><span dir="ltr"></span><span dir="ltr"></span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> MBC </span><span lang="AR-SA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">با روش رقت در براث تعیین گردید. در مطالعه مدل حیوانی، پس از 24 ساعت از آلوده شدن موش‌ها</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">به باکتری‌ها تیمار با تزریق 5/0 میلی لیتر از عصاره‌های</span><span dir="ltr"></span><span dir="ltr"></span><i><span lang="AR-SA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span></i><i><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr">SP Lecanora muralis </span></i><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr">(</span><span lang="AR-SA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">با غلظت</span><span dir="ltr"></span><span dir="ltr"></span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> MBC)</span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt mso-bidi-language: FA">انجام و</span><span dir="ltr"></span><span dir="ltr"></span><span lang="FA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">تعداد کلونی‌های باکتری‌های مذکور در طحال پس از گذشت 9 روز با کشت بر روی محیط آگاردار شمارش گردید</span><span dir="ltr"></span><span dir="ltr"></span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>.</span></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt text-align: justify line-height: normal unicode-bidi: embed direction: rtl" dir="rtl"><b><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt mso-bidi-language: FA">نتایج</span></b><span dir="ltr"></span><span dir="ltr"></span><b><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>:</span></b><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"> </span><span lang="AR-SA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">نتایج به دست آمده مشخص نمود که</span><span dir="ltr"></span><span dir="ltr"></span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> MIC </span><span lang="AR-SA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">عصاره استونی برای</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><i><span lang="AR-SA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">سالمونلا تیفی موریوم</span></i><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">و</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><i><span lang="AR-SA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">استافیلوکوکوس اورئوس</span></i><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">به</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt mso-bidi-language: FA">ترتیب75/103 و 5/207 میلی گرم بر میلی لیتر و عصاره اتانولی</span><span dir="ltr"></span><span dir="ltr"></span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> 81/49 </span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt mso-bidi-language: FA">و 625/99 میلی گرم بر میلی لیتر</span><span dir="ltr"></span><span dir="ltr"></span><span lang="FA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">بوده و عصاره آبی اثر ضد میکروبی بر روی باکتری‌ها نشان نداد. در مدل حیوانی میانگین تعداد باکتری‌های رشد کرده در</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt mso-bidi-language: FA">گروه‌های آزمایش نسبت به گروه‌های کنترل کاهش معنی دار نشان داد</span><span dir="ltr"></span><span dir="ltr"></span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>.</span></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt text-align: justify line-height: normal unicode-bidi: embed direction: rtl" dir="rtl"><b><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt mso-bidi-language: FA">نتیجه‌گیری</span></b><span dir="ltr"></span><span dir="ltr"></span><b><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>:</span></b><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt mso-bidi-language: FA">عصاره اتانولی</span><span dir="ltr"></span><span dir="ltr"></span><i><span lang="FA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span></i><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt mso-bidi-language: FA">در مقایسه با عصاره استونی فعالیت بیشتری داشته و</span><span dir="ltr"></span><span dir="ltr"></span><span lang="FA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><i><span lang="AR-SA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">سالمونلا تیفی موریوم</span></i><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">به عصاره‌ها حساس‌تر می‌باشد</span><span dir="ltr"></span><span dir="ltr"></span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>.</span></p><p class="MsoNormal" style="margin: 0cm 0cm 8pt"><span style="line-height: 107% font-family: "Times New Roman",serif font-size: 8pt mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt"><!--stripped--><font size="2"> </font><!--stripped--></span></p> <p><font face="Times New Roman" size="3"> </font><b><i><span style="color: black font-family: "Times New Roman",serif font-size: 10pt mso-fareast-font-family: "Times New Roman"">Background & Objective:</span></i></b><span style="color: black font-family: "Times New Roman",serif font-size: 10pt mso-fareast-font-family: "Times New Roman""> <i>Staphylococcus aureus</i> and <i>Salmonella typhimurium</i> are among important causes of infection and antibiotic resistance. It is essential to find antimicrobial compounds against them. The objective of this study is to evaluate the antibacterial activity of Lecanora muralis<b> </b>SP. extracts on <i>Staphylococcus aureu</i>s and <i>Salmonella typhimurium</i> in in-vitro and in animal model.</span></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt text-align: justify line-height: normal mso-layout-grid-align: none"><b><i><span style="color: black font-family: "Times New Roman",serif font-size: 10pt mso-fareast-font-family: "Times New Roman"">Materials & Methods:</span></i></b><b><span style="color: black font-family: "Times New Roman",serif font-size: 10pt mso-fareast-font-family: "Times New Roman""> </span></b><span style="color: black font-family: "Times New Roman",serif font-size: 10pt mso-fareast-font-family: "Times New Roman"">In this study, aquatic, acetonic, and ethanoic extracts of <i>Lecanora muralis<b> </b>SP</i>. were prepared. Then their antimicrobial effects were determined by agar well diffusion method. Minimum Inhibitory Concentration (MIC) and Minimum Bacterial Concentration (MBC) were calculated by macro dilution method. In animal model, 24 hours after inoculation of bacteria in mice, care was provided by injecting 0.5 ml of <i>Lecanora muralis<b> </b>SP</i>. extracts (as MBC concentration).. Then, the colonies of mentioned bacteria in spleen were counted on agar media after nine days.</span></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt text-align: justify line-height: normal mso-layout-grid-align: none"><b><i><span style="color: black font-family: "Times New Roman",serif font-size: 10pt mso-fareast-font-family: "Times New Roman"">Results:</span></i></b><span style="color: black font-family: "Times New Roman",serif font-size: 10pt mso-fareast-font-family: "Times New Roman""> The results indicated that MIC of acetonic extract of Lecanora muralis<b> </b>SP. for <i>Staphylococcus aureus</i> and <i>Salmonella typhimurium</i> were 103.75 and 207.5 mg/ml and for ethanoic extract were 49.81 and 99.625 mg/ml, respectively. The aquatic extracts of the tested lichens didn't show any antibacterial activity on mentioned organisms. The mean of the bacterial count in experiment group showed significant reduction compared to the control group in animal model.</span></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt text-align: justify line-height: normal mso-layout-grid-align: none"><b><i><span style="color: black font-family: "Times New Roman",serif font-size: 10pt mso-fareast-font-family: "Times New Roman"">Conclusion:</span></i></b><span style="color: black font-family: "Times New Roman",serif font-size: 10pt mso-fareast-font-family: "Times New Roman""> The ethanoic extract of <i>Lecanora muralis<b> </b>SP.</i> had the most effective antimicrobial activity on <i>Staphylococcus aureus</i> and <i>Salmonella typhimurium</i> in comparison with acetonic extract. In addition <i>Salmonella typhimurium</i> was more sensitive to extracts.</span></p> استافیلوکوکوس اورئوس, سالمونلا تیفی موریوم, عصاره‌های SP. Lecanora muralis, اثر ضد میکروبی. Staphylococcus aureus, Salmonella typhimurium, Lecanora muralis SP extracts, Antimicrobial effect. 318 326 http://jabs.fums.ac.ir/browse.php?a_code=A-10-53-2&slc_lang=fa&sid=1 Shahrzad Nasisri Semnani شهرزاد نصیری سمنانی Sh.nasiri92@yahoo.com 10031947532846005645 10031947532846005645 Yes Biological Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran. دانشگاه آزاد اسلامی، واحد زنجان، مرکزتحقیقات بیولوژی، زنجان، ایران. Mehdi Rahnema مهدی رهنما 10031947532846005646 10031947532846005646 No Biological Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran. دانشگاه آزاد اسلامی، واحد زنجان، مرکزتحقیقات بیولوژی، زنجان، ایران. Hasan Ghasempour حسن قاسم پور 10031947532846005647 10031947532846005647 No Biological Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran. دانشگاه آزاد اسلامی، واحد زنجان، مرکزتحقیقات بیولوژی، زنجان، ایران. Hamed Alizadeh حامد علیزاده 10031947532846005648 10031947532846005648 No Young Researchers and Elite Club, Zanjan Branch, Islamic Azad University, Zanjan, Iran. دانشگاه آزاد اسلامی، واحد زنجان، باشگاه پژوهشگران جوان و نخبگان، زنجان، ایران.