fa بررسی اثر عصاره هیدروالکلی موسیر ایرانی (Allium hirtifolium) بر میزان تشکیل هموگلوبین گلایکه شده در حالت برون تنی بيوشيمي بالینی Biochemistry پژوهشي Research <p><font face="Times New Roman" size="3">&nbsp;</font></p> <p class="MsoNormal" dir="rtl" style="margin: 0cm 0cm 10pt text-align: justify line-height: normal"><b><span 10pt="" 8.0pt="" b="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-hansi-font-family:="" new="" span="" style="color: black font-family: " times="">گلایکه شدن غیر آنزیمی، واکنش بین قندهای احیا کننده و گروه آمین پروتئین‌ها است. محصولات نهایی گلایکه شدن (</span><span 10.0pt="" 8pt="" b="" dir="ltr" font-size:="" mso-bidi-font-family:="" mso-bidi-font-size:="" new="" serif="" style="color: black font-family: " times="">AGE</span><span .="" 10.0pt="" 10pt="" 8.0pt="" 8pt="" b="" dir="ltr" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-hansi-font-family:="" new="" serif="" span="" style="color: black font-family: " times="">‌ها یا شکستن پیوند قند-پروتئین، در کاهش روند بهبود عوارض بیماری‌ها از جمله اختلالات دیابت موثر خواهد بود. در این مطالعه تاثیر عصاره هیدروالکلی موسیر ایرانی بر تشکیل هموگلوبین گلایکه شده در شرایط برون تنی بررسی گردید.<!--stripped--><!--stripped--></span></b></p> <p><b><font face="Times New Roman" size="3">&nbsp;</font></b></p> <p class="MsoNormal" dir="rtl" style="margin: 0cm 0cm 10pt text-align: justify line-height: normal"><b><b><span 10pt="" 8.0pt="" b="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-hansi-font-family:="" new="" span="" style="color: black font-family: " times="">تاثیر غلظت‌های 1/0، 3/0 و 5/0 گرم بر دسی لیتر عصاره موسیر ایرانی بر مهار تشکیل هموگلوبین </span><span 10.0pt="" 8pt="" b="" dir="ltr" font-size:="" mso-bidi-font-family:="" mso-bidi-font-size:="" new="" serif="" style="color: black font-family: " times="">A1c</span><span .="" 10.0pt="" 10pt="" 8.0pt="" 8pt="" b="" dir="rtl" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-hansi-font-family:="" new="" serif="" span="" style="color: black font-family: " times=""> با کروماتوگرافی تعویض یونی سنجش گردید</span><span .="" 10pt="" 8.0pt="" b="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-fareast-font-family:="" mso-hansi-font-family:="" new="" span="" style="color: black font-family: " times="">.</span><span 10.0pt="" 10pt="" 8.0pt="" 8pt="" b="" dir="rtl" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-fareast-font-family:="" mso-hansi-font-family:="" new="" p="" serif="" span="" style="color: black font-family: " times=""> نمایان گر اختلاف معنی دار بود.</span><span 10pt="" 8.0pt="" b="" class="MsoNormal" dir="rtl" face="Times New Roman" font="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-hansi-font-family:="" new="" p="" size="3" span="" style="color: black font-family: " times="">نتایج:</span></b><span 0="" 10.0pt="" 10pt="" 40="" 8.0pt="" 8pt="" b="" dir="rtl" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-hansi-font-family:="" new="" serif="" span="" style="color: black font-family: " times=""> پس از 7 و 14 روز انکوباسیون گردید. عصاره موسیر در تمام غلظت‌ها باعث کاهش مقدار هموگلوبین گلایکه شد. </span></b></p> <p class="MsoNormal" dir="rtl" style="margin: 0cm 0cm 10pt text-align: justify line-height: normal"><b><b><span 10pt="" 8.0pt="" b="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-hansi-font-family:="" new="" span="" style="color: black font-family: " times="">نتایج نشان داد موسیر می‌تواند مانع از تشکیل هموگلوبین </span><span 10.0pt="" 8pt="" b="" dir="ltr" font-size:="" mso-bidi-font-family:="" mso-bidi-font-size:="" new="" serif="" style="color: black font-family: " times="">A1c</span><span .="" 10pt="" 11.0pt="" 12pt="" 8.0pt="" b="" class="MsoNormal" dir="rtl" face="Times New Roman" font="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-hansi-font-family:="" new="" p="" size="3" span="" style="color: black line-height: 115% font-family: " times=""><font size="3">&nbsp;</font></span></b></b></p> <p><b><b><font face="Times New Roman" size="3">&nbsp;</font></b></b></p> <p><font face="Times New Roman" size="3">&nbsp;</font></p> <p class="MsoNormal" style="margin: 0cm 0cm 0pt text-align: justify line-height: normal unicode-bidi: embed direction: ltr text-autospace: text-justify: kashida text-kashida: 0% mso-layout-grid-align: none"><b><i><span 10pt="" font-size:="" mso-fareast-font-family:="" new="" serif="" style="color: black font-family: " times="">Background <span dir="rtl" lang="FA"><font face="Times New Roman" size="2">&</font></span> Objectives:</span></i></b><b><i><span font-size:="" new="" serif="" style="color: black font-family: " times="">&nbsp;</span></i></b><span font-size:="" new="" serif="" style="color: black font-family: " times="">Non enzymatic glycation is a reaction that occurs between reducing sugars and amino groups of proteins. Advanced Glycation End-products (AGE) have been accounted for principal biological processes like aging and pathogenesis of some diseases.</span><span 10pt="" font-size:="" mso-fareast-font-family:="" new="" serif="" style="color: black font-family: " times=""> Accumulation of </span><span font-size:="" new="" serif="" style="color: black font-family: " times="">AGE during hyperglycemia can cause structural and functional changes of long-lived proteins.</span><span 10pt="" font-size:="" mso-fareast-font-family:="" new="" serif="" style="color: black font-family: " times=""> Therefore, it will be effective to inhibit protein glycation formation in order to reduce or to improve diabetes complications</span><span font-size:="" new="" serif="" style="color: black font-family: " times="">. The aim of this study is to determine the effects of hydroalcoholic extract of Allium Hirtifolium on the formation of glycated hemoglobin.<span dir="rtl" lang="FA"><!--stripped--><!--stripped--></span></span></p> <p><font face="Times New Roman" size="3">&nbsp;</font><font face="Times New Roman" size="3">&nbsp;</font></p> <p class="MsoNormal" style="margin: 0cm 0cm 0pt text-align: justify line-height: normal unicode-bidi: embed direction: ltr text-autospace: text-justify: kashida text-kashida: 0% mso-layout-grid-align: none"><b><i><span 10pt="" font-size:="" mso-fareast-font-family:="" new="" serif="" style="color: black font-family: " times="">Materials <span dir="rtl" lang="FA"><font face="Times New Roman" size="2">&</font></span> Methods:</span></i></b><span 10pt="" font-size:="" mso-fareast-font-family:="" new="" serif="" style="color: black font-family: " times=""> In this experimental study</span><span font-size:="" new="" serif="" style="color: black font-family: " times="">, the effects of the various concentrations of 0.1, 0.3, and 0.5 gr/dl of hydroalcoholic extract of Allium Hirtifolium</span><span 10pt="" font-size:="" mso-fareast-font-family:="" new="" serif="" style="color: black font-family: " times=""> on the inhibition of hemoglobin glycation were examined. Hemoglobin A1c </span><span font-size:="" new="" serif="" style="color: black font-family: " times="">formation was assayed by ion exchange chromatography. The P-value < 0.05 was considered as the significant level.<span dir="rtl" lang="FA"><!--stripped--><!--stripped--></span></span></p> <p><font face="Times New Roman" size="3">&nbsp;</font><font face="Times New Roman" size="3">&nbsp;</font></p> <p class="MsoNormal" style="margin: 0cm 0cm 0pt text-align: justify line-height: normal unicode-bidi: embed direction: ltr text-autospace: text-justify: kashida text-kashida: 0% mso-layout-grid-align: none"><b><i><span 10pt="" font-size:="" mso-fareast-font-family:="" new="" serif="" style="color: black font-family: " times="">Results:</span></i></b><span 10pt="" font-size:="" mso-fareast-font-family:="" new="" serif="" style="color: black font-family: " times="">&nbsp;</span><span font-size:="" new="" serif="" style="color: black font-family: " times="">10 and 14 days after incubation, at the concentration of 40 millimollar glucose, hemoglobin A1c formation decreased significantly by using 0.5 gr/dl of hydroalcoholic extract of Allium Hirtifolium.<span dir="rtl" lang="FA"><!--stripped--><!--stripped--></span></span></p> <p><font face="Times New Roman" size="3">&nbsp;</font><font face="Times New Roman" size="3">&nbsp;</font></p> <p class="MsoNormal" style="margin: 0cm 0cm 0pt text-align: justify line-height: normal unicode-bidi: embed direction: ltr text-autospace: text-justify: kashida text-kashida: 0% mso-layout-grid-align: none"><b><i><span 10pt="" font-size:="" mso-fareast-font-family:="" new="" serif="" style="color: black font-family: " times="">Conclusion</span></i></b><b><i><span font-size:="" new="" serif="" style="color: black font-family: " times="">:</span></i></b><span font-size:="" new="" serif="" style="color: black font-family: " times=""> The results revealed that</span><span 10pt="" font-size:="" mso-fareast-font-family:="" new="" serif="" style="color: black font-family: " times="">&nbsp;</span><span font-size:="" new="" serif="" style="color: black font-family: " times="">Allium Hirtifolium </span><span 10pt="" font-size:="" mso-fareast-font-family:="" new="" serif="" style="color: black font-family: " times="">can inhibit hemoglobin </span><span font-size:="" new="" serif="" style="color: black font-family: " times="">A1c</span><span 10pt="" font-size:="" mso-fareast-font-family:="" new="" serif="" style="color: black font-family: " times=""> formation in </span><span font-size:="" new="" serif="" style="color: black font-family: " times="">hyperglycemic condition</span><span 10pt="" font-size:="" mso-fareast-font-family:="" new="" serif="" style="color: black font-family: " times="">. Therefore, it is suggested that </span><span font-size:="" new="" serif="" style="color: black font-family: " times="">Allium Hirtifolium</span><span 10pt="" font-size:="" mso-fareast-font-family:="" new="" serif="" style="color: black font-family: " times=""> can be useful for preventing the complications of diabetes.<span dir="rtl" lang="FA"><!--stripped--><!--stripped--></span></span></p> <p><font face="Times New Roman" size="3">&nbsp;</font></p> <p class="MsoNormal" style="margin: 0cm 0cm 0pt text-align: justify line-height: normal unicode-bidi: embed direction: ltr text-autospace: text-justify: kashida text-kashida: 0% mso-layout-grid-align: none"></p> <p><font face="Times New Roman" size="3">&nbsp;</font></p> دیابت ملیتوس, موسیر ایرانی, هموگلوبین A1c, هموگلوبین گلایکه شده Diabetes mellitus, Allium Hirtifolium, Hemoglobin A1c, Glycated hemoglobin 92 101 http://jabs.fums.ac.ir/browse.php?a_code=A-10-776-1&slc_lang=fa&sid=1 Shirin Fattahpour شیرین فتاح پور shirin_fattahpour@yahoo.com 1289209871 100319475328460011481 No Department of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. گروه بیوشیمی بالینی، دانشکده پزشکی، دانشگاه علوم پزشکی رفسنجان، رفسنجان، ایران. Farzaneh Hosseini فرزانه حسینی fhosseini.bio91@yahoo.com 100319475328460011482 100319475328460011482 No Department of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. گروه بیوشیمی بالینی، دانشکده پزشکی، دانشگاه علوم پزشکی رفسنجان، رفسنجان، ایران. Mohammad Reza Hajizadeh محمد رضا حاجی زاده hajizadehus@yahoo.com 100319475328460011483 100319475328460011483 No Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. مرکز تحقیقات پزشکی مولکولی، دانشگاه علوم پزشکی رفسنجان، رفسنجان، ایران. Mohammad Asadpour محمد اسد پور asadpoor1@yahoo.com 100319475328460011484 100319475328460011484 No Department of Social Medicine, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. گروه پزشکی اجتماعی، دانشکده پزشکی، دانشگاه علوم پزشکی رفسنجان، رفسنجان، ایران. مژگان موگویی ghassanshahi@gmail.com 100319475328460011485 100319475328460011485 No Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. مرکز تحقیقات پزشکی مولکولی، دانشگاه علوم پزشکی رفسنجان، رفسنجان، ایران. gholam hossein hassanshahi غلامحسین حسن شاهی mirzaeemr@gmail.com 100319475328460011486 100319475328460011486 No Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. مرکز تحقیقات پزشکی مولکولی، دانشگاه علوم پزشکی رفسنجان، رفسنجان، ایران. mohammad reza mirzaii محمد رضا میرزایی 100319475328460011487 100319475328460011487 No Department of Biophysics and Genetics, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. گروه بیوفیزیک و ژنتیک، دانشکده پزشکی، دانشگاه علوم پزشکی رفسنجان، رفسنجان، ایران. Mehdi Mahmoodi مهدی محمودی mahmoodies@yahoo.com 100319475328460011488 100319475328460011488 Yes Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. Department of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. گروه بیوشیمی بالینی، دانشکده پزشکی، دانشگاه علوم پزشکی رفسنجان، رفسنجان، ایران.