fa بررسی اثرات کورکومین بر بافت کبد در موش‌های صحرایی نر بالغ تحت درمان با سیکلوفسفامید پاتولوژي Pathology پژوهشي Research <p style="direction: rtl"><font face="Times New Roman" size="3"> </font><b><span lang="FA" style="color: black line-height: 200% font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA mso-ansi-font-size: 8.0pt">زمینه و هدف</span></b><span dir="ltr"></span><span dir="ltr"></span><b><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> : </span></b><span lang="FA" style="color: black line-height: 200% font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA mso-ansi-font-size: 8.0pt">سیکلوفسفامید یک داروی آنتی نئوپلاستیک است که علارغم کاربردهای کلینیکی فراوان در درمان سرطان، دارای اثرات سمی از طریق تولید رادیکال‌های آزاد بر سلول‌های بدن می‌باشد، بنابراین هدف از این مطالعه بررسی اثرات کورکومین به عنوان یک ماده آنتی اکسیدان بر بافت کبد می‌باشد</span><span dir="ltr"></span><span dir="ltr"></span><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>.</span><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><o:p /></span></p><p style="direction: rtl"><font face="Times New Roman" size="3"> </font><b><span lang="FA" style="color: black line-height: 200% font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA mso-ansi-font-size: 8.0pt">مواد و روش‌ها</span></b><span dir="ltr"></span><span dir="ltr"></span><b><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>: </span></b><span lang="FA" style="color: black line-height: 200% font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA mso-ansi-font-size: 8.0pt">تعداد 50 سر موش صحرایی نر بالغ از نژاد ویستار به طور تصادفی در 5 گروه (کنترل، شاهد (روغن زیتون+نرمال سالین)، گروه دریافت کننده</span><span dir="ltr"></span><span dir="ltr"></span><span lang="FA" style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA" style="color: black line-height: 200% font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">سیکلوفسفامید</span><span dir="ltr"></span><span dir="ltr"></span><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> (</span><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr">mg/kg</span><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"> 5 </span><span lang="AR-SA" style="color: black line-height: 200% font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">به صورت درون صفاقی)، گروه دریافت کننده کورکومین</span><span dir="ltr"></span><span dir="ltr"></span><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> (</span><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr">mg/kg</span><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"> 50)+ </span><span lang="AR-SA" style="color: black line-height: 200% font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">سیکلوفسفامید</span><span dir="ltr"></span><span dir="ltr"></span><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> (</span><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr">mg/kg</span><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"> 5) </span><span lang="AR-SA" style="color: black line-height: 200% font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">و گروه دریافت کننده کورکومین</span><span dir="ltr"></span><span dir="ltr"></span><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> (</span><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr">mg/kg</span><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"> 50</span><span lang="AR-SA" style="color: black line-height: 200% font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-ansi-font-size: 8.0pt">به صورت گاواژ</span><span dir="ltr"></span><span dir="ltr"></span><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>)</span><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr">) </span><span lang="FA" style="color: black line-height: 200% font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA mso-ansi-font-size: 8.0pt">قرار گرفتند. تیمار موش‌های صحرایی به مدت 30 روز ادامه داشت. در پایان پس از تشریح بافت کبد، مورد بررسی هیستوپاتولوژیک قرارگرفت</span><span dir="ltr"></span><span dir="ltr"></span><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>. </span></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt text-align: justify line-height: 200% unicode-bidi: embed direction: rtl" dir="rtl"><b><span lang="FA" style="color: black line-height: 200% font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA mso-ansi-font-size: 8.0pt">نتایج</span></b><span dir="ltr"></span><span dir="ltr"></span><b><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>: </span></b><span lang="FA" style="color: black line-height: 200% font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA mso-ansi-font-size: 8.0pt">آپوپتوز شدید، نکروز هپاتوسیت‌ها، پرخونی، التهاب موضعی و اندوتلیوزیس در گروه دریافت کننده سیکلوفسفامید مشاهده شد.اما در گروه تحت تیمار با سیکلوفسفامید+ کورکومین میزان التهاب موضعی و نکروز هپاتوسیت‌ها نسبت به گروه دریافت کننده سیکلوفسفامید کاهش چشمگیری داشته و پرخونی و اندوتلیوزیس جزئی و اندک بود و در گروه دریافت کننده کورکومین هیچ کدام از موارد فوق مشاهده نشد</span><span dir="ltr"></span><span dir="ltr"></span><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>.</span></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt text-align: justify line-height: 200% unicode-bidi: embed direction: rtl text-justify: kashida text-kashida: 0%" dir="rtl"><b><span lang="FA" style="color: black line-height: 200% font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA mso-ansi-font-size: 8.0pt">نتیجه‌گیری</span></b><span dir="ltr"></span><span dir="ltr"></span><b><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>:</span></b><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr"> </span><span lang="FA" style="color: black line-height: 200% font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA mso-ansi-font-size: 8.0pt">نتایج این تحقیق نشان می‌دهد</span><span dir="ltr"></span><span dir="ltr"></span><b><span lang="FA" style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span></b><span lang="FA" style="color: black line-height: 200% font-family: "B Nazanin" font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-ascii-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA mso-ansi-font-size: 8.0pt">مصرف همزمان سیکلوفسفامید و کورکومین می‌تواند اثرات ناشی از رادیکال‌های آزاد سیکلوفسفامید بر بافت کبد را کاهش دهد</span><span dir="ltr"></span><span dir="ltr"></span><b><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-family: "B Nazanin" mso-bidi-language: FA mso-bidi-font-size: 10.0pt" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>. </span></b></p><p><font face="Times New Roman" size="3"> </font></p> <font face="Times New Roman" size="3"> </font><p class="MsoNormal" style="margin: 0cm 0cm 0pt 13.5pt text-align: justify line-height: 200%"><b><i><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 10pt mso-fareast-font-family: "Times New Roman"">Background & Objective:</span></i></b><b><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 10pt mso-fareast-font-family: "Times New Roman""> </span></b><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 10pt mso-fareast-font-family: "Times New Roman"">Cyclophosphamide is an antineoplastic drug that has many clinical uses in cancer treatment, but it has toxic effects due to creation of free radicals. In this study, the effects of curcumin" as an antioxidant drug” on liver tissue was investigated.<o:p /></span></p><font face="Times New Roman" size="3"> </font><p class="MsoNormal" style="margin: 0cm 0cm 0pt 13.5pt text-align: justify line-height: 200%"><b><i><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 10pt mso-fareast-font-family: "Times New Roman"">Materials & methods:</span></i></b><b><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 10pt mso-fareast-font-family: "Times New Roman""> </span></b><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 10pt mso-fareast-font-family: "Times New Roman"">50 wistar adult male rats were selected randomly and were divided in to five groups including control, sham (receiving normal saline and olive oil), cyclophosphamide (5 mg/kg intraperitoneal), cyclophosphamide (5 mg/kg) + curcumin (50 mg/kg), and curcumin (50 mg/kg by gavage). </span><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA">This study continued for 30 days.</span><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 10pt mso-fareast-font-family: "Times New Roman""> The liver histopathological changes were investigated </span><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA">after the last administration.</span><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 10pt mso-fareast-font-family: "Times New Roman""><o:p /></span></p><font face="Times New Roman" size="3"> </font><p class="MsoNormal" style="margin: 0cm 0cm 0pt 13.5pt text-align: justify line-height: 200%"><b><i><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 10pt mso-fareast-font-family: "Times New Roman"">Results:</span></i></b><b><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 10pt mso-fareast-font-family: "Times New Roman""> </span></b><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 10pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA">Severe apoptosis, hepatocytes necrosis, congestion, focal inflammation, and endotheliosis were revealed in cyclophosphamide receiving group. However, focal inflammation and hepatocyte necrosis were decreased considerably in cyclophosphamide + curcumin group compared to cyclophosphamide group. Congestion and endotheliosis were very small. None of the above mentioned complications was observed in curcumin receiving group.</span><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 10pt mso-fareast-font-family: "Times New Roman""><o:p /></span></p><font face="Times New Roman" size="3"> </font><p class="MsoNormal" style="margin: 0cm 0cm 0pt 13.5pt line-height: 200%"><b><i><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 10pt mso-fareast-font-family: "Times New Roman"">Conclusion:</span></i></b><b><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 10pt mso-fareast-font-family: "Times New Roman""> </span></b><span style="color: black line-height: 200% font-family: "Times New Roman",serif font-size: 10pt mso-fareast-font-family: "Times New Roman"">The results of this study show the concurrent consumption of cyclophosphamide and curcumin can reduce the cyclophosphamide free radicals effects on liver tissue. <o:p /></span></p><font face="Times New Roman" size="3"> </font><p class="MsoNormal" style="margin: 0cm 0cm 8pt"><span style="line-height: 107% font-family: "Times New Roman",serif font-size: 10pt"><!--stripped--><font size="2"> </font><!--stripped--></span></p><font face="Times New Roman" size="3"> </font> کورکومین, کبد, سیکلوفسفامید, موش صحرایی, اثرات پاتولوژیک Curcumin, Liver, cyclophosphamide, Rat, Pathologic effects 344 352 http://jabs.fums.ac.ir/browse.php?a_code=A-10-673-1&slc_lang=fa&sid=1 Zahra khodaparast زهرا خداپرست Zahra.khodaparast91@yahoo.com 2372460511 10031947532846005662 Yes Department of Biology, Arsanjan Branch, Islamic Azad University, Arsanjan, Iran. گروه زیست شناسی، دانشگاه آزاد اسلامی واحد ارسنجان، ارسنجان، ایران. ali reza yousofi علی رضا یوسفی pathosvet@yahoo.com 10031947532846005663 10031947532846005663 No Department of Pathological, School of Veterinary Medicine, kazerun branch, Islamic Azad University, kazerun, Iran. گروه پاتولوژی، دانشکده دامپزشکی، دانشگاه آزاد اسلامی، واحد کازرون، کازرون، ایران. ameneh khoshvagti آمنه خوشوقتی khoshvaghti2004@yahoo.com 10031947532846005664 10031947532846005664 No Department of Clinical Sciences, Faculty of Veterinary Medicine, Kazerun branch, Islamic Azad University, Kazerun, Iran. گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه آزاد اسلامی، واحد کازرون، کازرون، ایران.