en آنالیز تاثیر ترکیبات عصاره اتانولی و متانولی یونجه بر رده سلولی K562 داروسازي و فارماکولوژِی Pharmacology پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><u><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Background </span></span></span></u></b><b><u><span dir="RTL" lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">&</span></span></span></u></b><b><u><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496"> Objectives</span></span></span></u></b><b><u><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">:</span></span></span></u></b> <span new="" roman="" style="font-family:" times="">For centuries, the alfalfa (<i>Medicago sativa</i> L.) plant has been recognized for its versatile and active role in treating various diseases. Not only has it been utilized as a therapeutic agent, but it has also been served as a dietary component for both animals and humans. Given the distinctive attributes of this plant in ethnopharmacology, this study aimed to investigate the effects of ethanolic and methanolic extracts of <i>M. sativa</i> L. on the K562 myeloid cell line under <i>in vitro</i> conditions.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><u><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Materials & Methods:</span></span></span></u></b> <span new="" roman="" style="font-family:" times="">The phytochemical composition of <i>M. sativa</i> L. was determined through Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Cell viability was assessed using the MTT assay, wherein K562 cells were subjected to varying concentrations (50&ndash;100 &mu;g/mL) of methanolic and ethanolic extracts over 24, 48, and 72-hour intervals to determine the IC50. Subsequently, the most promising IC50 result was employed in flow cytometry (Flow Jo Software) analysis.</span><span lang="EN-GB" style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><u><span lang="EN-GB" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Results:</span></span></span></u></b> <span new="" roman="" style="font-family:" times="">Active constituents identified included phytol, phenol, linolenic acid, and glycine. Statistical analysis revealed a time-dependent but not dose-dependent effect. It was noteworthy that the IC50 for the methanolic extract after 72 hours was 9.45 &micro;g/mL, whereas it was 19.3 &micro;g/mL for the ethanolic extract. Flow cytometry analysis indicated that the methanolic extract caused 49.16% and the ethanolic extract caused 15.42% of cell death.</span><span lang="EN-GB" style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><u><span lang="EN-GB" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Conclusion</span></span></span></u></b><b><u><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">:</span></span></span></u></b> <span new="" roman="" style="font-family:" times="">The results demonstrated that the ethanolic extract of alfalfa is more effective than the methanolic extract on the K562 cell line. Therefore, <i>M. sativa</i> L. potential application in myeloid cancer therapy can be investigated in more details.</span></span></span></span></div> Medicago sativa L. Chemical compound, Cancer cell line, K562, Herbal medicine 232 243 http://jabs.fums.ac.ir/browse.php?a_code=A-10-1076-5&slc_lang=en&sid=1 Mahdieh Raeeszadeh مهدیه رئیس زاده vet_mr@yahoo.com 100319475328460029268 100319475328460029268 Yes Department of Basic Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran گروه علوم پایه، واحد سنندج، دانشگاه آزاد اسلامی، سنندج، ایران Afsoon Afshari افسون افشاری afssonafshar@gmail.com 100319475328460029269 100319475328460029269 No Department of Molecular Genetics, Shiraz Nephro-Urology Research Center, Shiraz University, Shiraz, Iran گروه ژنتیک مولکولی، مرکز تحقیقات نفرو-اورولوژی شیراز، دانشگاه شیراز، شیراز، ایران Samaneh Kazemiafshar سمانه کاظمی افشار samanehkazemi2@yahoo.com 100319475328460029270 0009-0004-3028-7599 No Faculty of Veterinary Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran دانشکده علوم دامپزشکی، واحد سنندج، دانشگاه آزاد اسلامی، سنندج، ایران Hajar Kazemiafshar هاجر کاظمی افشار hkazemiafshar@gmail.com 100319475328460029271 0009-0003-6330-117X No Faculty of Veterinary Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran دانشکده علوم دامپزشکی، واحد سنندج، دانشگاه آزاد اسلامی، سنندج، ایران Ali Akbar Amiri علی اکبر امیری a.amiri@yahoo.com 100319475328460029272 0009-0009-4709- No Department of Basic Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran گروه علوم پایه، واحد سنندج، دانشگاه آزاد اسلامی، سنندج، ایران