Journal of Advanced Biomedical Sciences

en Larvicidal activity of Melissa officinalis and Rosmarinus officinalis extracts and its lethal impacts on detoxifying enzymes in larvae of Aedes aegypti L. عفوني Infectious disease پژوهشي Research <div style="text-align: justify;">Background & Objectives: Mosquito-borne diseases significantly impact global health, particularly in tropical regions. While synthetic insecticides are currently employed to control mosquito vectors, their detrimental effects on ecosystems and persistence necessitate alternative control methods. Botanicals, owing to their diverse phytocompounds, offer potential for controlling and preventing vector-borne diseases by targeting insect eggs and larvae. This study aimed to evaluate the toxicity of Melissa officinalis and Rosmarinus officinalis extracts (methanolic and aqueous) against Aedes aegypti larvae, a vector of arboviruses. Materials & Methods: Total protein content in control and plant extract-exposed larval homogenates was estimated using bovine serum albumin as a standard. Acetylcholinesterase (AChE) and carboxylesterase assays were performed to determine larvicidal effects. Larval mortality was assessed after 24 hours of exposure. Results: Our findings revealed that the methanolic leaf extract of M. officinalis exhibited superior larvicidal activity (100% at 1000 ppm) compared to the methanolic R. officinalis extract (84±2.45% at 1000 ppm). In contrast, the aqueous extracts of both plant species inferred no larvicidal activity. The LC50 and LC90 values for M. officinalis methanolic extract were 378.7 ppm and 795.8 ppm, respectively, whereas those for R. officinalis were 648.9 ppm and 1152.9 ppm, respectively. Furthermore, biochemical assays measuring total protein, acetylcholinesterase, α-carboxylesterase, and β-carboxylesterase activities were conducted for M. officinalis, corroborating and substantiating its larvicidal properties. Conclusion: This study demonstrates that the methanolic leaf extract of M. officinalis possesses significant larvicidal efficacy against A. aegypti. These findings suggest that this plant or its phytocompounds could serve as a bioinsecticide, offering a potential alternative to environmentally toxic and non-biodegradable synthetic insecticides.  </div> Aedes aegypti, Medicinal plants, Bioinsecticide, Acetylcholinesterase, carboxylesterase, Marker enzymes 190 199 http://jabs.fums.ac.ir/browse.php?a_code=A-10-1709-19&slc_lang=en&sid=1 Mohd Abass Sofi محمد عباس صوفی 100319475328460029251 100319475328460029251 No Department of Chemistry, Sathyabama Institute of Science and Technology, Rajiv Gandhi Salai, Chennai- 600119, India گروه شیمی، موسسه علوم و فناوری ساتیاباما، راجیو گاندی سالای، چنای- 600119، هند S. Sunitha S. Sunitha سانیتها سانیتها 100319475328460029252 100319475328460029252 No Department of Chemistry, Sathyabama Institute of Science and Technology, Rajiv Gandhi Salai, Chennai- 600119, India گروه شیمی، موسسه علوم و فناوری ساتیاباما، راجیو گاندی سالای، چنای- 600119، هند Mohmmad Ashaq Sofi محمدعشاق صوفی 100319475328460029253 100319475328460029253 No Department of Biomedical Engineering, Sathyabama Institute of Science and Technology, Rajiv Gandhi Salai, Chennai- 600119, India گروه مهندسی زیست پزشکی، موسسه علم و فناوری ساتیاباما، راجیو گاندی سالای، چنای- 600119، هند Anish Mariadhas آنیش ماریاهاس 100319475328460029254 0009-0002-5430-3966 No Department of Mechanical Engineering, Sathyabama Institute of Science and Technology, Rajiv Gandhi Salai, Chennai- 600119, India گروه مهندسی مکانیک، موسسه علم و فناوری ساتیاباما، راجیو گاندی سالای، چنای- 600119، هند Jayakumar Manickkam جایاکومار مانیکهام 100319475328460029255 100319475328460029255 No Unit of Applied Entomology, Department of Zoology, University of Madras, Guindy Campus, Chennai -600025, India واحد حشره شناسی کاربردی، گروه جانورشناسی، دانشگاه مدرس، پردیس گیندی، چنای -600025، هند Ezhumalai Parthiban اژومالای پارتیبان 100319475328460029256 100319475328460029256 No Department of Zoology, Unit of Aquatic Biodiversity, University of Madras, Guindy Campus, 600025, Chennai, India گروه جانورشناسی، واحد تنوع زیستی آبزیان، دانشگاه مدرس، پردیس گیندی، 600025، چنای، هند Ramachandran Maduraiveeran راماچاندران مادورایران 100319475328460029257 100319475328460029257 No Unit of Applied Entomology, Department of Zoology, University of Madras, Guindy Campus, Chennai -600025, India واحد حشره شناسی کاربردی، گروه جانورشناسی، دانشگاه مدرس، پردیس گیندی، چنای -600025، هند Abdolmajid Ghasemian عبدالمجید قاسمیان majidghasemian86@gmail.com 100319475328460029258 100319475328460029258 Yes Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran مرکز تحقیقات بیماری‌های غیرواگیر، دانشگاه علوم پزشکی فسا، فسا، ایران