Journal of Advanced Biomedical Sciences

en Myrtle Essential Oil-Loaded Alginate Nanoparticles: A Dual Therapeutic Approach for Cytotoxicity Against Skin Cancer Cells and Antibacterial Effects on Common Pathogens نانوتکنولوژی پزشکی Nanomedicine پژوهشي Research <div style="text-align: justify;">Background & Objectives: The skin, being the body's largest organ, is not only susceptible to one of the most prevalent forms of cancer but also vulnerable to a myriad of pathogens, including bacteria. Myrtle essential oil (EO) has been shown to possess both anticancer and antimicrobial properties. This study aimed to investigate the efficacy of alginate nanoparticles containing myrtle EO on melanoma (A375) and epidermoid carcinoma (A431) cell lines, as well as on some common bacteria, including Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. Materials & Methods: Initially, myrtle EO was analyzed using Gas Chromatography-Mass Spectrometry. Subsequently, alginate nanoparticles were prepared via the ionic-gelation method and characterized by dynamic light scattering, Zeta potential, and attenuated total reflectance-Fourier transform infrared spectroscopy. Encapsulation efficacy was then determined using UV-Vis spectrometry. Both cytotoxicity assessment (MTT assay) and evaluation of antibacterial effects (microdilution assays) were conducted using the 96-well plate format. Results: The major compounds identified in myrtle EO were α-pinene, 1,8-cineole, linalool, linalool acetate, and geranyl acetate. The alginate nanoparticles exhibited a size of 160 ± 9 nm, a SPAN of 0.96, and a Zeta potential of -26 ± 2 mV. The encapsulation efficacy was determined to be 78.4%. It was found that the nanoparticles demonstrated cytotoxicity against A375 and A431 cells with IC50 values of 211 µg/mL and 308 µg/mL, respectively. The most potent antibacterial effect was observed against S. aureus (IC50: 266 µg/mL). Conclusion: The alginate nanoparticles containing myrtle EO, which exhibited notable cytotoxicity against melanoma and epidermoid carcinoma cells as well as potent antibacterial effects, show promise for further investigation in vivo studies.  </div> Nanotechnology, Complementary Medicine, Cytotoxicity, Melanoma 177 189 http://jabs.fums.ac.ir/browse.php?a_code=A-10-2124-2&slc_lang=en&sid=1 Hamid Moradi حمید مرادی moradyhamid7@gmail.com 100319475328460029246 0009-0002-1437-5578 No Student Research Committee, Fasa University of Medical Sciences, Fasa, Iran کمیته تحقیقات دانشجویی، دانشگاه علوم پزشکی فسا، فسا، ایران Mojdeh Safari مژده صفری mojdeh_safari64@yahoo.com 100319475328460029247 100319475328460029247 No Finetech in Medicine Research Center, School of Medicine, Iran University of Medical Sciences, Tehran, Iran مرکز تحقیقات ریزفناوری در پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی ایران، تهران، ایران Razieh Ranjbar راضیه رنجبر ranjbarrazie@gmail.com 100319475328460029248 100319475328460029248 No Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran گروه بیوتکنولوژی پزشکی، دانشکده فناوری‌های پیشرفته پزشکی، دانشگاه علوم پزشکی فسا، فسا، ایران Karrar S. Zayed کرار زاید karrars.alshebly@uokufa.edu.iq 100319475328460029249 100319475328460029249 No Department of Laboratory Investigations, Faculty of Science, University of Kufa, Najaf, Iraq گروه تحقیقات آزمایشگاهی، دانشکده علوم، دانشگاه کوفه، نجف، عراق Mahmoud Osanloo محمود اوصانلو osanloo_mahmood@yahoo.com 100319475328460029250 100319475328460029250 Yes Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran گروه نانوتکنولوژی پزشکی، دانشکده فناوری‌های پیشرفته پزشکی، دانشگاه علوم پزشکی فسا، فسا، ایران