en تحلیل زیست‌سازگاری ECM مشتق از طحال موش بافت شناسي Histology پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><u><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Background & Objectives:</span></span></span></u></b> <span new="" roman="" style="font-family:" times="">This study aimed to construct a decellularized mouse spleen scaffold and evaluate its cellular compatibility <em>in vitro</em> using murine bone marrow-derived mesenchymal stem cells (BM-MSCs).</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><u><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Materials & Methods:</span></span></span></u></b> <span new="" roman="" style="font-family:" times="">A combination of physical, chemical, and enzymatic treatments was employed for mouse spleen decellularization. These included multiple freeze-thaw cycles, the ionic detergent sodium dodecyl sulfate (SDS), and enzymatic trypsin. Histological and scanning electron microscopy analyses were conducted up to 7 days post-culture to assess the impact of decellularization and cellular adaptation to the spleen scaffolds.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><u><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Results:</span></span></span></u></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Histological studies revealed the attachment and penetration of BM-MSCs into the scaffolds on days 5-7 following cell seeding. Furthermore, cell migration into the scaffold was observed 5 days after the seeding process.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><u><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Conclusion:</span></span></span></u></b> <span new="" roman="" style="font-family:" times="">The decellularization approach utilized in this study proved to be effective and biocompatible, supporting the preservation and proliferation of BM-MSCs. These findings indicate its potential for spleen tissue engineering applications.</span><b><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif""></span></span></b></span></span></span><br> &nbsp;</div> Decellularization, Scaffold, Spleen tissue, Extracellular matrix, Mesenchymal stem cells, Tissue engineering 210 221 http://jabs.fums.ac.ir/browse.php?a_code=A-10-3384-1&slc_lang=en&sid=1 Yasin Ghabool یاسین قبول yasinghabool@gmail.com 100319475328460029260 100319475328460029260 No Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran گروه زیست شناسی، دانشکده علوم، واحد مشهد، دانشگاه آزاد اسلامی، مشهد، ایران Mahshid Reghbati مهشید رغبتی mahshidreghbati23@gmail.com 100319475328460029261 100319475328460029261 No Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran گروه زیست شناسی، دانشکده علوم، واحد مشهد، دانشگاه آزاد اسلامی، مشهد، ایران Nasser Mahdavi Shahri ناصر مهدوی شهری mahdavin@um.ac.ir 100319475328460029262 100319475328460029262 No Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran گروه زیست شناسی، دانشکده علوم، دانشگاه فردوسی مشهد، مشهد، ایران Amin Tavassoli امین توسلی Tavassoli.am@gmail.com 100319475328460029263 100319475328460029263 Yes Department of Biotechnology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran گروه بیوتکنولوژی، دانشکده دامپزشکی، دانشگاه فردوسی مشهد، مشهد، ایران