en عملکرد نانولوله کربنی بارگیری شده با تاموکسیفن و پتانسیل ضد سرطانی آن در برابر سلول های سرطانی پستان انسان بيولوژي سلولی-مولكولي Cellular-Molecular Biology پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Background & Objectives:</span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> Carbon nanotubes (CNTs) serve as molecular carriers for in vivo and in vitro delivery. Initial studies have suggested that nanotubes in drug delivery can enhance the therapeutic response to anti-cancer drugs. The present study intended to investigate the effect of CNTs carrying tamoxifen (TAM-CNTs) on the induction of apoptosis in the MDA-MB-231 cell line.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Materials & Methods:</span></span></span></b><b> </b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">The cells were treated with various concentrations of TAM and TAM-CNTs. The IC50 for these compounds was determined using a MTT assay. The cells were then treated with a lower concentration of IC50. The <i>BAX</i> and <i>BCL-2 </i>genes expression were evaluated by Real-Time PCR and Western blot. Flow cytometry was employed for evaluating apoptosis induction by TAM and TAM-CNTs.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Results:</span></span></span></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">The IC50 value of TAM and TAM-CNTs in a 48-hour period was 66.19 mg/mL and 36.59 mg/mL, respectively. The results demonstrated that BAX in the cells treated with TAM and TAM-CNTs was upregulated 3.64 and 7.88 times, respectively (P <0.05). Conversely, BCL-2 was downregulated 3.98 and 5.31 times (P <0.05). Furthermore, Western blot experiments confirmed the expression of BAX and BCL-2 proteins based on their gene expression. Flow cytometry results indicated that the viability of MDA-MB-231 cells in the control group, TAM-treated, and TAM-CNTs-treated cells was 95.3%, 64.9%, and 13.75%, respectively. This suggests that TAM-CNTs significantly diminishes cell viability compared to TAM (P <0.001).</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Conclusion:</span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> The findings revealed that TAM accompanied by CNTs exhibits a greater cytotoxic and apoptotic effect on MDA-MB-231 cells.</span></span></span></span></span><br> &nbsp;</div> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-family:"Times New Roman","serif""></span></b></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><span style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span> Tamoxifen, Carbon Nanotubes, Nano-delivery system, Apoptosis 1 11 http://jabs.fums.ac.ir/browse.php?a_code=A-10-1211-3&slc_lang=en&sid=1 Syavash Yazdian Anari سیاوش یزدیان اناری syavashyazdian@gmail.com 100319475328460028403 100319475328460028403 No Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran گروه زیست شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران Nafiseh Saminasab نفیسه سامی نسب nafisesami@gmail.com 100319475328460028404 100319475328460028404 No Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran گروه زیست شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران Mina Ramezani مینا رمضانی mina.ramezani@gmail.com 100319475328460028405 100319475328460028405 Yes Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran گروه زیست شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران Azadeh Mohammadgholi آزاده محمدقلی a.mohammadgholi@yaho.com 100319475328460028406 100319475328460028406 No Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran گروه زیست شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران