en شناسایی کوکسیلا بورنتی براساس ژن com1 با استفاده از تکنیک Real-time loop mediated isothermal amplification ميكروبیولوژی Microbiology پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#1f497d">Background & Objectives:</span></span></span></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">The present study designed a loop mediated isothermal amplification (LAMP) method to rapidly detect <i>Coxiella burnetii</i><b> </b>(<i>C. burnetii</i>)<b> </b>and develop a sensitive Real-time quantitative LAMP (Q-LAMP) assay to quantify Q fever. </span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#1f497d">Materials & Methods:</span></span></span></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Primers were specifically designed for use in targeting the <i>com1</i> conserved gene of <i>C. burnetii </i>to carry out LAMP detection of the agent causing Q fever. After obtaining the LAMP reaction, the sensitivity of the method was assessed by preparing a serial ten-fold dilution of a plasmid carrying <i>com1</i> gene. </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#1f497d">Results:</span></span></span></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">The assay&rsquo;s sensitivity for the visual detection of changes in turbidity or turbidimeter and electrophoresis of agarose gel were 0.4 fg and 0.04 fg, respectively. Hence, the lower limit of detection was 120 and 12 copies of the gene detected in 60 min. The results of this study were indicative of the simplicity, rapidness, sensitivity, and specificity of the LAMP assay for <i>C. burnetii</i> detection and a probable improvement of the diagnostic procedure used in clinical laboratories. </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#1f497d">Conclusion:</span></span></span></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">The assay specificity was assessed using <i>Coxiella</i> genomic DNA and a panel of Gram-negative and Gram-positive bacteria. The LAMP assay was shown to be highly specific for detection of <i>Coxiella</i> without any observable amplification products from non-<i>Coxiella</i> organisms.</span></span></span></span></span></span></span></div> <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#1f497d"></span></span></span></b></span></span></span></span></span><br> <br> <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><span style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span></span> Coxiella burnetii, Q fever, Loop mediated isothermal amplification (LAMP) 12 20 http://jabs.fums.ac.ir/browse.php?a_code=A-10-2134-7&slc_lang=en&sid=1 Razieh Zabihi راضیه ذبیحی 100319475328460028439 100319475328460028439 No Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran گروه میکروبیولوژی، واحد قم، دانشگاه آزاد اسلامی، قم، ایران Keivan Majidzadeh-A کیوان مجیدزاده 100319475328460028440 100319475328460028440 No Academic Center for Education, Culture & Research (ACECR), the Breast Cancer Research Center (BCRC), Tehran, Iran مرکز آموزشی، فرهنگی و تحقیقاتی دانشگاهی (ACECR)، مرکز تحقیقات سرطان پستان (BCRC)، تهران، ایران Abbas Morovvati عباس مروتی 100319475328460028441 100319475328460028441 No Department of Microbiology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran گروه میکروبیولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی آجا، تهران، ایران Mohammad Soleimni محمد سلیمانی 100319475328460028442 100319475328460028442 Yes Infectious Diseases Research Center, AJA University of Medical Sciences, Tehran, Iran مرکز تحقیقات بیماری‌های عفونی، دانشگاه علوم پزشکی آجا، تهران، ایران