Journal of Advanced Biomedical Sciences

en Phytochemical and Cytotoxic Properties of Gum and Leaf Extracts of Ferula assafoetida بيوشيمي بالینی Biochemistry پژوهشي Research <div style="text-align: justify;">Background & Objectives: Breast cancer, a significant threat to women's health, is associated with a high annual mortality rate. Oxidative stress, characterized by the accumulation of reactive oxygen species, plays a pivotal role in cancer development. Ferula assafoetida, a traditional medicinal plant, has been widely used in cancer treatment. This study aims to compare the cytotoxic effects, phytochemical profiles, and antioxidant activities of Ferula assafoetida gum (FAGE) and leaf (FALE) extracts. Materials & Methods: The herbal extracts from FAGE and FALE were prepared using the maceration method in 70% v/v ethanol. The phenolic content and radical scavenging activity of the extracts were assessed using the Folin-Ciocalteu and diphenylpicrylhydrazyl (DPPH) assays, respectively. The cytotoxic effects of the extracts were examined on the MDA-MB-231 and MCF-7 cell lines through the MTT assay. Additionally, the morphological changes of the cell lines were observed using an inverted microscope. Results: The phenolic content in FAGE (142.25± 19.35 µgGAE/mg) was significantly higher (P=0.01) than that in FALE (48.15± 3.01 µgGAE/mg). Additionally, the radical scavenging activity and cytotoxic effects of FAGE were also significantly stronger than those of FALE (P< 0.05). Regarding the cytotoxic effects of the extracts, FAGE exhibited IC50 values of 80.59± 10.98 and 59.39± 15.89 µg/mL for MCF-7 and MDA-MB-231 cells, respectively, after 72 hours of incubation. Conclusions: FAGE exhibited a greater cytotoxic effect on the MDA-MB-231 cell line than on the MCF-7, possibly following an estrogen-independent pattern. It appears that FAGE holds significant potential in refining oxidative stress, thereby inhibiting cell proliferation.</div>   Phenol, Flavonoid, Oxidative stress, Cytotoxicity, Cancer 329 339 http://jabs.fums.ac.ir/browse.php?a_code=A-10-1409-4&slc_lang=en&sid=1 Alireza Moulazadeh علیرضا مولازاده immunomoula@gmail.com 100319475328460028218 100319475328460028218 No Noncommunicable Disease Research Center, Fasa University of Medical Sciences, Fasa, Iran مرکز تحقیقات بیماری‌های غیرواگیر، دانشگاه علوم پزشکی فسا، فسا، ایران Razieh Ranjbar راضیه رنجبر ranjbarrazie@gmail.com 100319475328460028219 100319475328460028219 No Noncommunicable Disease Research Center, Fasa University of Medical Sciences, Fasa, Iran. مرکز تحقیقات بیماری‌های غیرواگیر، دانشگاه علوم پزشکی فسا، فسا، ایران Amin Dakhili Ardestani امین دخیلی اردستانی amindakhili114@gmail.com 100319475328460028220 100319475328460028220 No Student Research Committee, Fasa University of Medical Sciences, Fasa, Iran کمیته تحقیقات دانشجویی، دانشگاه علوم پزشکی فسا، فسا، ایران Kamran Ranjbar کامران رنجبر kamranranjbar40@gmail.com 100319475328460028221 100319475328460028221 No Student Research Committee, Fasa University of Medical Sciences, Fasa, Iran کمیته تحقیقات دانشجویی، دانشگاه علوم پزشکی فسا، فسا، ایران Seyed Amin Kouhpayeh سید امین کوهپایه kouhpayeha@gmail.com 100319475328460028222 100319475328460028222 No Noncommunicable Disease Research Center, Fasa University of Medical Sciences, Fasa, Iran مرکز تحقیقات بیماری‌های غیرواگیر، دانشگاه علوم پزشکی فسا، فسا، ایران Maryam Maghbool مریم مقبول maghbol.maryam@yahoo.com 100319475328460028223 100319475328460028223 No Noncommunicable Disease Research Center, Fasa University of Medical Sciences, Fasa, Iran مرکز تحقیقات بیماری‌های غیرواگیر، دانشگاه علوم پزشکی فسا، فسا، ایران Sohrab Najafipour سهراب نجفی پور resimmuno@gmail.com 100319475328460028224 100319475328460028224 Yes Noncommunicable Disease Research Center, Fasa University of Medical Sciences, Fasa, Iran مرکز تحقیقات بیماری‌های غیرواگیر، دانشگاه علوم پزشکی فسا، فسا، ایران Fatemeh Najafipour فاطمه نجفی پور 100319475328460028225 0009-0004-7193-9338 No Ghutbuddin Clinic, Shiraz University of Medical Sciences, Shiraz, Iran درمانگاه قطب الدین، دانشگاه علوم پزشکی شیراز، شیراز، ایران