fa بررسی اثرتنوع ژن‌های ایمنی IL-18 و IFN-γ برشدت بیماری مالاریا و کیفیت درمان اپيدميولوژي Epidemiology پژوهشي Research <p><span lang="FA" style="color: black font-family: "><!--stripped--><b><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA">زمینه و هدف</span></b><span dir="ltr"></span><span dir="ltr"></span><b><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>:</span></b><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"> </span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA">در مالاریای انسانی، ژنوتیپ سایتوکاین‌ها در پروسه دفاع سیستم ایمنی نقش دارند. در این مطالعه ارتباط بین ژنوتیپ</span><span dir="ltr"></span><span dir="ltr"></span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>-137G/C </span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA">در</span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr">IL-18</span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA">و</span><span dir="ltr"></span><span dir="ltr"></span><span lang="FA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr">+874A/T </span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA">در</span><span dir="ltr"></span><span dir="ltr"></span><span lang="FA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr">IFN-γ</span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"> </span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA">بر شدت بیماری مالاریا و نیز کیفیت درمان - به عنوان فاکتورهای موثر در ایجاد افراد بدون علامت در مناطق اندمیک</span><span dir="ltr"></span><span dir="ltr"></span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>- </span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA">بررسی گردید</span><span dir="ltr"></span><span dir="ltr"></span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>.</span><span dir="rtl"></span><span dir="rtl"></span><span style="color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman""><span dir="rtl"></span><span dir="rtl"></span> </span><!--stripped--></span></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt text-align: justify line-height: normal unicode-bidi: embed direction: rtl" dir="rtl"><b><span lang="FA" style="background: white color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA">مواد و روش‌ها</span></b><span dir="ltr"></span><span dir="ltr"></span><b><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>:</span></b><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"> </span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA">در این تحقیق 100 نفر بیمار و 102 نفر سالم با روش</span><span dir="ltr"></span><span dir="ltr"></span><span lang="FA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr">Nested –PCR</span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"> </span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA">و گسترش ضخیم مورد ارزیابی قرار گرفتند. ژنوتیپ افراد برای</span><span dir="ltr"></span><span dir="ltr"></span><span lang="FA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr">IL-18(G-137C)</span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"> </span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA">با روش</span><span dir="ltr"></span><span dir="ltr"></span><span lang="FA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr">Single Specific Primer-Polymerase Chain Reaction </span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA">و برای</span><span dir="ltr"></span><span dir="ltr"></span><span lang="FA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr">IFN-γ(A+874T)</span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"> </span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA">با</span><span dir="ltr"></span><span dir="ltr"></span><span lang="FA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr">ARMS-PCR</span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"> </span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA">تعیین گردید و نتایج مورد آنالیز آماری قرار گرفتند</span><span dir="ltr"></span><span dir="ltr"></span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>.</span></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt text-align: justify line-height: normal unicode-bidi: embed direction: rtl" dir="rtl"><b><span lang="FA" style="background: white color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA">نتایج</span></b><span dir="ltr"></span><span dir="ltr"></span><b><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>:</span></b><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"> </span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA">در این تحقیق، مشاهده گردید که آلل</span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr">T </span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA">در ژن</span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr">IFN-γ </span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA">و آلل</span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr">C </span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA">در ژن</span><span dir="ltr"></span><span dir="ltr"></span><span lang="FA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr">IL-18</span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"> </span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA">اثر محافظت کننده‌ای را القاء می‌نمایند. همچنین مشاهده گردید که آنمی ناشی از مالاریا، ملایم‌تر و تعداد پارازیت در میکرو لیتر خون به طور معنی داری کاهش یافته بود</span><span dir="ltr"></span><span dir="ltr"></span><span lang="FA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr">(p<0.0001)</span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr">. </span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA">این موضوع در پاسخ بیمار به درمان نیز دیده شد به طوری که پارازیت در خون دارندگان ژنوتیپ</span><span dir="ltr"></span><span dir="ltr"></span><span lang="FA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr">IFN-γTT</span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"> </span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA">و</span><span dir="ltr"></span><span dir="ltr"></span><span lang="FA" style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr">IL-18CC</span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"> </span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA">در پایان روز دوم درمان دیده نشد</span><span dir="ltr"></span><span dir="ltr"></span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>.</span></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt text-align: justify line-height: normal unicode-bidi: embed direction: rtl" dir="rtl"><b><span lang="FA" style="background: white color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA">نتیجه‌گیر</span></b><b><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA">ی</span></b><span dir="ltr"></span><span dir="ltr"></span><b><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>:</span></b><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"> </span><span lang="FA" style="color: black font-family: "B Nazanin" font-size: 10pt mso-ansi-font-size: 8.0pt mso-ascii-font-family: "Times New Roman" mso-fareast-font-family: "Times New Roman" mso-hansi-font-family: "Times New Roman" mso-bidi-language: FA">یافته‌های فوق، تئوری تاثیر ژنوتیپ سیستم ایمنی بر کیفیت درمان و امکان ایجاد افراد بدون علامت و ذخیره انگلی به عنوان فاکتور موثر در اندمیک شدن مالاریا را مطرح می‌کند. بنابراین، پیشنهاد می‌گردد که ایمنوژنوتیپ افراد یک منطقه نیز می‌تواند به عنوان عامل مهمی در استراتژی‌هایی که به منظور کنترل بیماری مالاریا در مناطق اندمیک لحاظ می‌گردد، در نظر گرفته شود</span><span dir="ltr"></span><span dir="ltr"></span><span style="color: black font-family: "Times New Roman",serif font-size: 8pt mso-fareast-font-family: "Times New Roman" mso-bidi-language: FA mso-bidi-font-size: 10.0pt mso-bidi-font-family: "B Nazanin"" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>.</span></p><font face="Times New Roman" size="3"></font><!--stripped--> <p class="MsoNormal" style="margin: 0cm 0cm 10pt text-align: justify line-height: normal unicode-bidi: embed direction: ltr text-justify: kashida text-kashida: 0%"><b><i><span style="color: black font-family: ">Background & Objective:</span></i></b><span style="color: black font-family: "> In human malaria, genotype of cytokines affect immune system. The impact of genotype -137G/C in IL-18 and +874A/T in IFN-γ on the severity of malaria and the quality of treatment – as factors to form asymptomatic persons in endemic regions- has been investigated.</span></p><p class="MsoNormal" style="margin: 0cm 0cm 10pt text-align: justify line-height: normal unicode-bidi: embed direction: ltr text-justify: kashida text-kashida: 0%"><b><i><span style="color: black font-family: ">Materials & Methods:</span></i></b><span style="color: black font-family: "> 100 patients and 102 healthy persons were evaluated by Nested-PCR and thick blood film. IL-18(G-137C) and IFN- γ (A+874T) were determined by Single Specific Primer-Polymerase Chain Reaction and ARMS-PCR methods, respectively. The results were analyzed statistically.<o:p /></span></p><font face="Times New Roman" size="3"></font><p class="MsoNormal" style="margin: 0cm 0cm 10pt text-align: justify line-height: normal unicode-bidi: embed direction: ltr text-justify: kashida text-kashida: 0%"><b><i><span style="color: black font-family: ">Results:</span></i></b><span style="color: black font-family: "> In this research, it has been observed that allele-T in IFN- γ gene and allele-C in IL-8 gene have <font size="2"><font face="Times New Roman"><span style="mso-spacerun: yes"> </span>protective effects against severity of Malaria. In addition, it has clearly been found that development and growth rate of plasmodium in </font></font></span><span style="color: black font-family: ">µ</span><span style="color: black font-family: ">l of blood as well as anemia induced malaria significantly reduced in carriers of these alleles (p<0.0001).<font size="2"><font face="Times New Roman"><span style="mso-spacerun: yes"> </span>This effect has also been observed in the quality of the treatment in such a way that no parasite remained at the end of second day of treatment in carriers of alleles IFN- γTT and IL-18CC.<span style="mso-spacerun: yes"> </span><o:p /></font></font></span></p><font face="Times New Roman" size="3"></font><p class="MsoNormal" style="margin: 0cm 0cm 10pt text-align: justify line-height: normal unicode-bidi: embed direction: ltr text-justify: kashida text-kashida: 0%"><b><i><span style="color: black font-family: ">Conclusion:</span></i></b><span style="color: black font-family: "> These findings issue the impact of immunogenetypes of patients on treatment quality and asymptomatic patients and parasite reservoir as effective factors in endemic malaria. Immunogenetypes of people may play as a remarkable factor to control malaria in endemic areas.</span></p><font face="Times New Roman" size="3"></font> مالاریا, IL-18, IFN-γ, ژنوتیپ و ذخیره انگلی Malaria, IL-18, IFN-γ˛ Genotype, Parasite Reservoir 284 294 http://jabs.fums.ac.ir/browse.php?a_code=A-10-320-1&slc_lang=fa&sid=1 Gholam-Reza Ameri غلامرضا عامری dameri@rocketmail.com 3390270302 10031947532846005627 No Molecular Medicine Research Centre, Hormozgan University of Medical Science, Bandar Abbas, Iran. مرکز تحقیقات پزشکی مولکولی، دانشگاه علوم پزشکی هرمزگان، بندرعباس، ایران. Kianoosh Malekzadeh کیانوش ملک زاده keyanoosh@gmail.com 0059991135 10031947532846005628 Yes Molecular Medicine Research Centre, Hormozgan University of Medical Science, Bandar Abbas, Iran. دانشکده علوم پایه، واحد علوم و تحقیقات دانشگاه آزاد اسلامی، تهران، ایران. Zahra Noor-Mohammadi زهرا نورمحمدی 10031947532846005629 10031947532846005629 No Department of Biology-Genetics, Faculty of Basic sciences, Science Research Branch, Islamic Azad University, Tehran, Iran. دانشکده علوم پایه – واحد علوم و تحقیقات دانشگاه آزاد اسلامی Mohammad Shekari محمد شکاری 10031947532846005630 10031947532846005630 No Molecular Medicine Research Centre, Hormozgan University of Medical Science, Bandar Abbas, Iran. دانشکده علوم پایه، واحد علوم و تحقیقات دانشگاه آزاد اسلامی، تهران، ایران. Habibollah Turki حبیب الله ترکی 10031947532846005631 10031947532846005631 No Molecular Medicine Research Centre, Hormozgan University of Medical Science, Bandar Abbas, Iran. دانشکده علوم پایه، واحد علوم و تحقیقات دانشگاه آزاد اسلامی، تهران، ایران. Hanieh Solaimani هانیه سلیمانی 10031947532846005632 10031947532846005632 No Molecular Medicine Research Centre, Hormozgan University of Medical Science, Bandar Abbas, Iran. دانشکده علوم پایه، واحد علوم و تحقیقات دانشگاه آزاد اسلامی، تهران، ایران.