en آنالیز بیان FOXO2 و ASCL1 در موشهای تیمار شده با زعفران جنين شناسي Embryology پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Background & Objective:</span></span></span></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">The aim of this study was to investigate the effect of saffron extract on the embryo development of mice. </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Materials & Methods:</span></span></span></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Pregnant NMRI mice were randomized into control and treatment groups at 25, 50, and 100 mg/kg saffron doses. Saffron extract was administered to mice by gavage on days 7-12 of pregnancy. On the 17th day of pregnancy, the embryos were removed from the uterus and their weight and height measured. Moreover, their brain tissue has been evaluated histologically. Subsequently, the expression of the <i>Foxp2</i> and <i>Ascl1</i> genes in the brain tissue was assessed using the real-time PCR method. </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Results:</span></span></span></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">All embryos were aborted in mothers that received 100 mg/kg of saffron. At dose 50 mg/kg, only embryos of a mother reached the end of pregnancy. Embryos treated with 25 mg/kg saffron were significantly heavier than controls (P<0.05). Furthermore, the tail length was significantly shorter (P<0.05). Histological findings showed that there was no difference between the control and treated groups, and the brain tissue was well developed. <i>Foxp2</i> and <i>Ascl1</i> genes were significantly overexpressed in both the 25 and 50 mg/kg treatment groups compared to the control group (P<0.05). </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Conclusion: </span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">The results of this study showed that saffron extract can have significant effects in low concentrations (25 mg/kg) on the development of the mouse embryos as well as the expression of <i>Foxp2</i> and <i>Ascl1</i> genes. </span></span></span></span></span></span></div> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></b></span></span></span><br> &nbsp; saffron, embryo, mouse, Foxp2 gene, Ascl1 gene 175 184 http://jabs.fums.ac.ir/browse.php?a_code=A-10-1211-2&slc_lang=en&sid=1 Syavash Yazdian سیاوش یزدیان syavashyazdian@gmail.com 100319475328460027749 0009-0008-5983-4460 No Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran گروه زیست شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران Mina Ramezani مینا رمضانی mina.ramezani@gmail.com 100319475328460027750 100319475328460027750 Yes Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran گروه زیست شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران Nastaran Asgarimoghadam نسترن اصغری مقدم namoghadam@gmail.com 100319475328460027751 100319475328460027751 No Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran گروه زیست شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران