en بيولوژي سلولی-مولكولي Cellular-Molecular Biology مروری Review <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">CRISPR is an extraordinarily powerful technique regulating any target gene across the genome with promising therapy intentions. CRISPR-Cas9 is a convenient tool for gene manipulation. Notwithstanding this, the broad consequence of human gene editing, particularly germinal genes, cannot be predicted. Firstly, once edited, the genes would be part of the human population for successive generations and may be impossible to remove from humanity; secondly, success is not guaranteed; thirdly, the fidelity of editing, as it could affect unrelated genes or unspecified segments of DNA; and last but not least, its influence on gene interaction, network, and signaling pathways could be difficult to be predicted. CRISPR-Cas9 mostly includes precise genome editing, rapidity and cost-effectiveness, creation of disease models, study of gene function, applications in gene therapy and translation research and wide diversity for species. The technique also ignited the moral and ethical concerns of scientific community.&nbsp; Ethics and safety approval for gene modification in human cells is required by the National Institutes of Health (NIH). The NIH does not currently fund studies of CRISPR in human embryos and opposes the CRISPR utilization in germline cells because these alterations would be permanent and heritable. The technology has promised with the most profound implications for cancer therapy. Recent advances in CRISPR-based technology is redefining how cancer is studied and potentially improves anti-cancer therapies. One way to improve the technology is to use machine-learning approaches to comprehending CRISPR errors and predicting more specific edits and repairing outcomes. </span></span></span></span></span><br> &nbsp;</div> CRISPR-Cas9, genome editing, animal modeling, advantages, disadvantages   252 264 http://jabs.fums.ac.ir/browse.php?a_code=A-10-1709-10&slc_lang=en&sid=1 Abdolmajid Ghasemian عبدالمجید قاسمیان majidghasemian86@gmail.com 100319475328460028200 100319475328460028200 Yes Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran مرکز تحقیقات بیماری‌های غیرواگیر، دانشگاه علوم پزشکی فسا، فسا، ایران Ensieh Nournia انسیه نورنیا 100319475328460028201 100319475328460028201 No Cardiologist at Be'sat Hospital, Hamedan University of Medical Sciences, Hamedan, Iran متخصص قلب و عروق، بیمارستان بعثت، دانشگاه علوم پزشکی همدان، همدان، ایران Ghasem Nikfar قاسم نیکفر 100319475328460028202 100319475328460028202 No Clinical Research Development Unit, Valiasr Hospital, Fasa University of Medical Sciences, Fasa, Iran واحد توسعه تحقیقات بالینی، بیمارستان ولیعصر، دانشگاه علوم پزشکی فسا، فسا، ایران Maryam Kohansal مریم کهنسال 100319475328460028203 100319475328460028203 No Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran مرکز تحقیقات بیماری‌های غیرواگیر، دانشگاه علوم پزشکی فسا، فسا، ایران Mohammad Ghorbani محمد قربانی 100319475328460028204 100319475328460028204 No Department of Pathology, School of Allied Medicine Sciences, Fasa University of Medical Sciences, Fasa, Iran گروه آسیب شناسی، دانشکده علوم پزشکی وابسته، دانشگاه علوم پزشکی فسا، فسا، ایران Afsaneh Salahi افسانه صالحی 100319475328460028205 100319475328460028205 No Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran مرکز تحقیقات بیماری‌های غیرواگیر، دانشگاه علوم پزشکی فسا، فسا، ایران Mojtaba Memariani مجتبی معماریان 100319475328460028206 100319475328460028206 No Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran مرکز تحقیقات بیوتکنولوژی، انستیتو پاستور ایران، تهران، ایران