en ژنوتایپینگ جدایه های انسانی بروسلا ملی تنسیس جدا شده از غرب ایران به روش MLVA. عفوني Infectious disease پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:10.0pt"><span style="background:whitesmoke"><span new="" roman="" style="font-family:" times=""><span style="color:black">Background & Objective:</span></span></span></span></i></b> <span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Brucellosis is an endemic disease with a high prevalence in Iran whose highest frequency is in the western region of the country. Genetic diversity investigation is an important method to determine the epidemiological relationship of <i>Brucella</i> isolates in different geographical areas. Therefore, the present study aimed to investigate the genetic diversity of human <i>Brucella melitensis</i> (<i>B. melitensis</i>) strains using the <span style="background:whitesmoke"><span style="color:black">Multiple Locus Variable-Number Tandem Repeat Analysis</span></span> (MLVA) Typing method in the west of the country.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:10.0pt"><span style="background:whitesmoke"><span new="" roman="" style="font-family:" times=""><span style="color:black">Materials & Methods</span></span></span></span></i></b><b><span style="font-size:10.0pt"><span style="background:whitesmoke"><span new="" roman="" style="font-family:" times=""><span style="color:black">:</span></span></span></span></b><span style="font-size:10.0pt"><span style="background:whitesmoke"><span new="" roman="" style="font-family:" times=""><span style="color:black"> In this study, 20 strains of isolated <i>B. melitensis</i> were collected from the human serum samples of suspected Brucellosis in the west of the country and were analyzed by MLVA-16 method.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:10.0pt"><span style="background:whitesmoke"><span new="" roman="" style="font-family:" times=""><span style="color:black">Results:</span></span></span></span></i></b><span style="font-size:10.0pt"><span style="background:whitesmoke"><span new="" roman="" style="font-family:" times=""> The results showed that 3 genotype numbers 42, 43 and 47 were identified using MLVA-8 method and using MLVA-11 method genotypes 125, 138 and 111 were recognized. Also, 16 different genotypes were detected from the analysis of the isolates by MLVA-16 method which shows a high degree of polymorphism among the isolates due to the high genetic diversity of the isolates in Panel 2B loci.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:10.0pt"><span style="background:whitesmoke"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></span></i></b><span style="font-size:10.0pt"><span style="background:whitesmoke"><span new="" roman="" style="font-family:" times=""> The results showed the high genetic diversity of <i><span style="color:black">B. melitensis</span></i> isolates in the west of the country and their genetic relationship with the known strains in the neighboring countries of the Eastern Mediterranean area, as well as the importance of the MLVA method in identifying the source of infection.</span></span></span></span></span></span></div> Brucellosis, Brucella melitensis, Genotype, MLVA, Human, Iran 431 438 http://jabs.fums.ac.ir/browse.php?a_code=A-10-3203-1&slc_lang=en&sid=1 Heidar Rahimi حیدر رحیمی heidarrahimi@yahoo.com 4209694721 100319475328460026886 No Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khoramabad, Iran گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه لرستان، خرم آباد، ایران Ehsean Rashidian احسان رشیدیان rashidian.e@lu.ac.ir 100319475328460026887 100319475328460026887 Yes Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khoramabad, Iran گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه لرستان، خرم آباد، ایران Nemat Shams نعمت شمس nematshams1386@yahoo.com 100319475328460026888 100319475328460026888 No Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khoramabad, Iran گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه لرستان، خرم آباد، ایران Amin Jaydari امین جایدری jaydari.a@lu.ac.ir 100319475328460026889 100319475328460026889 No Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khoramabad, Iran گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه لرستان، خرم آباد، ایران