en سودوموناس آئروژینوزا: برآوردی از وقوع ژن های بیماری زا، OprL و ToxA در نمونه های بالینی انسانی و دامپزشکی ميكروبیولوژی Microbiology پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><i><span lang="EN-GB" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Background & Objective:</span></span></span></i></b><b> </b><span lang="EN-GB" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">An opportunistic pathogen <i>Pseudomonas aeruginosa</i> can cause frequent hospital-acquired infections as well as one microorganism in the food spoilage. Also, the emergence of multidrug-resistant <i>P. aeruginosa</i> has become a serious threat to public health.This pathogen has many virulence factors which aid in bacterial invasion as well as toxicity, during infections. Out of different virulence genes in <i>Pseudomonas</i> <i>aeruginosa</i>, <i>opr</i>L (</span></span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">E</span></span></span><span lang="EN-GB" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">ncoding membrane lipoprotein L) and <i>tox</i>A (encoding exotoxin A i.e. ETA), are predominantly involved in, <i>P. aeruginosa</i>-related infections.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><i><span lang="EN-GB" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Materials & Methods:</span></span></span></i></b><b> </b><span lang="EN-GB" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">A total of 120 specimens of the bacteria <i>Pseudomonas aeruginosa</i> were collected from Veterinary microbiology and various hospital laboratories. The isolates were initially identified by culturing on MacConkey agar and Eosin Methylene blue (EMB) agar and were further characterized by morphological and biochemical tests. An antibiotic sensitivity test was carried out on 13 antibiotics using the disc diffusion method. Genotypic detection of <i>opr</i>L and <i>tox</i>A genes was performed using a specific PCR test. </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><i><span lang="EN-GB" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Results:</span></span></span></i></b><b> </b><span lang="EN-GB" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">The results revealed that the <i>tox</i>A gene was detected by 84.62% in isolates belonging to human samples and 75% in the isolates of animal samples, whereas the <i>opr</i>L gene was detected by 80.77% and only 16.67 % in the isolates were derived from human and animal samples, respectively. </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><i><span lang="EN-GB" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Conclusion:</span></span></span></i></b><b> </b><span lang="EN-GB" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">The PCR analysis can help in the fast and specific detection of <i>opr</i>L and <i>tox</i>A genes in <i>P. aeruginosa</i>. Monitoring of these pathogenic genes could prevent the risk of transmission of multi-drug resistant <i>P. aeruginosa</i>, from animals to humans. </span></span></span></span></span></span><br> &nbsp;</div> Pseudomonas aeruginosa, antibiotics, multi-drug resistance, lipoprotein, exotoxin, PCR 412 421 http://jabs.fums.ac.ir/browse.php?a_code=A-10-3151-1&slc_lang=en&sid=1 Ciamak Ghazaei سیامک قضایی ciamakghazaei@yahoo.com 100319475328460026869 100319475328460026869 Yes Department of Microbiology, University of Mohaghegh Ardabili, Ardabil, Iran گروه میکروبیولوژی، دانشگاه محقق اردبیلی، اردبیل، ایران