en کلونیزاسیون و مقاومت آنتی بیوتیکی استافیلوکوکوس اورئوس بینی در کارکنان بهداشتی و درمانی جنوب غرب ایران: رخداد OS-MRSA ميكروبیولوژی Microbiology پژوهشي Research <span style="font-size:12pt"><span style="line-height:normal"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt">Background & Objectives:</span></b><span style="font-size:10.0pt"> <i>Staphylococcus</i> spp. is a resident flora of the skin and mucosa of humans that can colonize the anterior nares of individuals. This cross-sectional study was conducted to determine the rate and antibiotic resistance pattern of nasal <i>Staphylococcus aureus</i> (<i>S. aureus</i>) carriers among the staff of Fasa hospital, southern Iran.</span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:normal"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt">Materials & Methods:</span></b><span style="font-size:10.0pt"> Nasal swab samples were collected from 117 hospital staff working in 12 wards. Microbiological culture method was applied for <i>S. aureus</i> identification. The isolates were confirmed by <i>tuf</i> gene identification using PCR assay. Five isolates were randomly sequenced and phylogenetically analysed </span>&nbsp;<span style="font-size:10.0pt">using MEGA software. The antimicrobial resistance pattern of the isolates was evaluated using the disc diffusion assay and the amplification of the methicillin resistance (<i>mecA</i>) gene. </span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:normal"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt">Results:</span></b><span style="font-size:10.0pt"> The prevalence of <i>S. aureus</i> nasal carriers included 10.26% (n=12). The nasal carriers were identified in the wards of surgery ICU, gynecologic surgery, NICU, pediatric, internal surgery, and emergency. Among them, gynecologic surgery staff had the highest rate of nasal colonization (33.33%). Phylogenetic analysis showed that of five isolates, four had high similarities with each other. Also, the highest rate of resistance was related to penicillin (83.3%), followed by cefazolin (75%), and cephalexin (75%). However, the highest level of susceptibility (100%) was found for vancomycin, cefoxitin, and oxacillin. Furthermore, the methicillin resistance gene (<i>mecA</i>) was highly detected (75%) from the isolates, elucidating oxacillin-susceptible or cefoxitin-susceptible <i>mecA</i>-positive <i>S. aureus</i> (OS-MRSA). </span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:normal"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt">Conclusions:</span></b><span style="font-size:10.0pt"> T</span><span style="font-size:10.0pt">he high rates of OS-MRSA can lead to antibiotic resistance among health care workers tremendously. Moreover, the high similarity probability in phylogenetic analysis shows the possibility of cross-infection between these health care workers, warning to exert effective strategies to control infection spread, especially in the surgery ward. </span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:normal"><span new="" roman="" style="font-family:" times=""><span style="font-size:10.0pt"></span></span></span></span><br> <br> <div></div> Staphylococcus aureus, OS-MRSA, MecA gene, Healthcare workers,Colonization 388 396 http://jabs.fums.ac.ir/browse.php?a_code=A-10-1670-2&slc_lang=en&sid=1 Abbas Abdollahi عباس عبداللهی a.abdollahi360@yahoo.com 100319475328460026850 100319475328460026850 No Department of Medical Microbiology, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran گروه میکروبیولوژی پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی فسا، فسا، ایران Zahra Montaseri زهرا منتصری montaserizahra90@gmail.com 100319475328460026851 100319475328460026851 Yes Department of Infectious Diseases, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran گروه بیماری‌های عفونی، دانشکده پزشکی، دانشگاه علوم پزشکی فسا، فسا، ایران Seyed Saeid Yazdanparast سید سعید یزدان پرست saeidyp72@gmail.com 100319475328460026852 100319475328460026852 No School of Medicine, Fasa University of Medical Sciences, Fasa, Iran دانشکده پزشکی، دانشگاه علوم پزشکی فسا، فسا، ایران Maryam Montaseri مریم منتصری m.pourmontaseri@shirazu.ac.ir 100319475328460026853 100319475328460026853 No Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran گروه بهداشت و سلامت مواد غذایی، دانشکده دامپزشکی، دانشگاه شیراز، شیراز، ایران