Journal of Advanced Biomedical Sciences
مجله علوم زیست پزشکی پیشرفته
JABS
Medical Sciences
http://jabs.fums.ac.ir
1
admin
2228-5105
2783-1523
8
7
14
8888
13
en
jalali
1401
8
1
gregorian
2022
11
1
12
4
online
1
fulltext
en
The Association of cagA, vacA, babA2, babB and oipA of Helicobacter pylori with Risk of Gastric Carcinoma Development
انكولوژي
Oncology
پژوهشي
Research
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Background & Objective:</span></span></i></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> Helicobacter pylori (H. pylori), carried by more than half of the world population, is a major cause of chronic duodenal and gastric ulcers, gastritis and carcinoma. Colonization and toxin production include major virulence traits of H. pylori. The aim of this study was to assess the existence of H. pylori and virulence factors among patients with risk of gastrointestinal carcinoma (GC) in an Iraqi population. </span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Materials & Methods: </span></span></i></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">During May 2016- October 2020 in Babylon, Iraq, a total of 500 biopsy samples were obtained from gastric tissue of patients with GC, gastritis, duodenitis, duodenal ulcer and gastric ulcer and cultured onto the Brucella agar. H. pylori isolates were identified using conventional biochemical and molecular tests. Molecular identification was conducted by amplification of glmM gene using the polymerase chain reaction (PCR) technique. The adhesin (babA2, babB and oipA) and toxin (cagA and vacA) genes were also amplified using PCR technique. </span></span></span></span></span></span></div>
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Results: </span></span></i></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Among 500 biopsy samples, 269 (110 from males and 159 from female patients) H. pylori isolates were identified. The age range of patients was 14-69 years (mean age=47.34±7.23). The babA2 and babB genes were detected in 59.47% and 59.10% of isolates, respectively. Notably, babA2 was observed in 89% of GC and 64% of DN strains being significantly more associated with GC and DN (<0.0001 and 0.028, respectively). Furthermore, babB-positive strains were significantly (0.042) more associated with PG. The rate of cagA and vacA was 44.60% and 48.32%, respectively. The cagA was detected in 64.73% of GC, and 100% of PG and DN strains with a significant association. We detected the oipA in 58.36% of strains which was significantly associated with GC (74%, P=0.0001), PG (88%, p<0.0001) and DN (84%, p<0.0001) as compared to oipA-negative strains.</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Conclusion: </span></span></i></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">The existence of H. pylori babA2, cagA and oipA virulence genes was associated with GC, DN and PG. As these genes play a crucial role in the development of gastric carcinoma, accurate control measure toward hindering the colonization of pathogenic strains is essential. </span></span></span></span></span></span></div>
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:red"></span></span></span></i></b></span></span></span></span></div>
Helicobacter pylori, colonization, virulence, gastric carcinoma, duodenal cancer
406
411
http://jabs.fums.ac.ir/browse.php?a_code=A-10-1709-5&slc_lang=en&sid=1
Zainab
H. Alsaadi
زینب
السعدی
alsaadi.z@gmail.com
100319475328460026863
100319475328460026863
No
Department of Biology, College of Medicine, University of Babylon, Iraq
گروه زیست شناسی، دانشکده پزشکی، دانشگاه بابل، عراق
Nada
Kadlim Hindi
ندا
خزعل
nada.kh@gmail.com
100319475328460026864
100319475328460026864
No
Department of Biology, College of Medicine, University of Babylon, Iraq
گروه زیست شناسی، دانشکده پزشکی، دانشگاه بابل، عراق
Ali H.
Al Marzoqi
علی
المرزوقی
almarzoqiali80@gmail.com
100319475328460026865
100319475328460026865
No
Department of Biology, College of Science for Women, University of Babylon, Iraq
گروه زیست شناسی، دانشکده علوم زنان، دانشگاه بابل، عراق
Mojtaba
Memariani
مجتبی
معماریانی
100319475328460026866
100319475328460026866
No
Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
مرکز تحقیقات بیوتکنولوژی، انستیتو پاستور ایران، تهران، ایران
Maryam
Kohansal
مریم
کهنسال
100319475328460026867
100319475328460026867
No
Noncommunicable diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran
مرکز تحقیقات بیماریهای غیرواگیر، دانشگاه علوم پزشکی فسا، فسا، ایران
Abdolmajid
Ghasemian
عبدالمجید
قاسمیان
majidghasemian86@gmail.com
100319475328460026868
100319475328460026868
Yes
Noncommunicable diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran
مرکز تحقیقات بیماریهای غیرواگیر، دانشگاه علوم پزشکی فسا، فسا، ایران