Journal of Advanced Biomedical Sciences

en Evaluation of LncRNA uc.173 and Occludin in Iranian Patients with Inflammatory Bowel Disease Compared with Healthy Individuals بيولوژي سلولی-مولكولي Cellular-Molecular Biology پژوهشي Research <div style="text-align: justify;">Background & Objective: Inflammatory bowel disease (IBD) is characterized by the chronic gastrointestinal inflammation. The two common forms of IBD are ulcerative colitis (UC) and Crohn’s disease (CD) that are distinguished by their location and depth of involvement in the diffuse inflammation of the colonic mucosa and affects the rectum (proctitis). A novel class of LncRNAs transcribed from ultra-conserved regions (UCRs) is a recently identified ultra-conserved region (T-UCR) transcript that is involved in the cellular function in a variety of pathways. However, the regulation of LncRNA uc.173 in IBD remains to be fully elucidated. In this study, we aimed to examine the expression of LncRNA uc.173 and Occludin genes in an Iranian population with inflammatory bowel disease. Materials & Methods: This case-control study was performed on 33 inflammatory bowel disease patients including 13 Crohn's disease, 20 ulcerative colitis and 20 healthy controls. The mRNA levels of LncRNA uc.173 and Occludin genes were assessed using the quantitative Real-time polymerase chain reaction. The B2M was used as an internal control. The 2 -ΔΔCq method was used to determine the expression fold changes.</div> <div style="text-align: justify;">Results: Statistically, the level of the LncRNA uc.173 gene expression between the UC and normal tissues increased significantly(P=0.0024). Also, the expression analysis revealed no significant difference between the samples of CD patients compared to the controls (P>0.05). In order to further evaluate the role of LncRNA uc.173 in IBD, the associations between the transcript levels of the LncRNA uc.173 and Occludin mRNA demonstrated significant difference in the CD tissue (R=0.59; P=0.002). In our study, the mRNA expression of Occludin gene did not show any changes in the IBD patients compared to the healthy controls. Conclusion: The increased expression of LncRNA uc.173 in the tissues of UC patients may be considered as a diagnostic or prognostic biomarker. Also, there was no correlation found between Occludin and LncRNA uc.173 expressions in the IBD patients' tissues.  </div> Inflammatory bowel disease, Ulcerative colitis, Crohn's disease, LncRNA uc.173, Occludin 215 222 http://jabs.fums.ac.ir/browse.php?a_code=A-10-3056-1&slc_lang=en&sid=1 Vahid Chaleshi وحید چالشی chaleshi@gmail.com 100319475328460026099 100319475328460026099 No Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran مرکز تحقیقات اپیدمیولوژی پایه و مولکولی اختلالات گوارشی، پژوهشکده بیماری‌های گوارش و کبد، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران Saeideh Oloumi Kalantar سعیده علومی کلانتر s.oloumi1373@gmail.com 100319475328460026100 100319475328460026100 No Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران Iman Salahshourifar ایمان سلحشوری فر isalahshouri@gmail.com 100319475328460026101 100319475328460026101 No Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران Shabnam Shahrokh شبنم شاهرخ shabnamshahrokh@gmail.com 100319475328460026102 100319475328460026102 No Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran مرکز تحقیقات اپیدمیولوژی پایه و مولکولی اختلالات گوارشی، پژوهشکده بیماری‌های گوارش و کبد، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران Hamid Asadzadeh Aghdaei حمید اسدزاده hamid.assadzadeh@gmail.com 100319475328460026103 100319475328460026103 Yes Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran مرکز تحقیقات اپیدمیولوژی پایه و مولکولی اختلالات گوارشی، پژوهشکده بیماری‌های گوارش و کبد، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران Mohammad Reza Zali محمد رضا زالی nnzali@hotmail.com 100319475328460026104 100319475328460026104 No Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran مرکز تحقیقات بیماری‌های گوارش و کبد، پژوهشکده بیماری‌های گوارش و کبد، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران