en بيولوژي سلولی-مولكولي Cellular-Molecular Biology پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Background & Objective</span></span></i></b><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">: </span></span></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Breast cancer is a phenotypically complex and diverse genetic disease caused by changes in the structure and expression of specific genes. Immune system factors are also involved in the etiology of this neoplasm. This study aimed to evaluate the effect of genetic changes (rs2243250 & rs2227284) on the interleukin 4 gene and body mass index on breast cancer risk in Iranian women.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Material & Methods:</span></span></i></b><b> </b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">From women referring to Shohada-Tajrish Hospital, 100 women with breast cancer and 100 healthy women were selected. After blood sampling and DNA extraction, the women&rsquo;s genotypes were determined using the RFLP-PCR technique. The results were evaluated by SPSS software version 21 and chi-square and logistic regression tests.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></i></b><b> </b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Analysis of the results with different genetic models showed the effect of rs2243250 on breast cancer </span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">(p<0.05)</span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">, but rs2227284 was not associated with breast cancer (p>0.05). People with the CC / TT genotype (polymorphism) were more likely to get breast cancer. Also, the increase in body mass index was significantly associated with both polymorphisms studied. Also, carriers of the TT genotype of rs2243250 polymorphism were more likely to develop breast cancer with aging.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></i></b><b> </b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Genetic alterations in the IL-4 gene and obesity probably contribute to breast cancer, and carriers of both genetic modifications (CC / TT) are more likely to develop breast cancer.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:10.0pt"><span style="font-family:"Times New Roman","serif""></span></span></b></span></span></span><br> &nbsp;</div> Interleukin 4, rs2243250, rs2227284, Breast Cancer, Polymorphisms 140 151 http://jabs.fums.ac.ir/browse.php?a_code=A-10-116-4&slc_lang=en&sid=1 Samanehsadat Shahidi سمانه سادات شهیدی samanehshahidi93@gmail.com 100319475328460026068 100319475328460026068 No Department of Medical Genomics Research Center, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran گروه مرکز تحقیقات ژنومیک پزشکی، علوم پزشکی تهران، دانشگاه آزاد اسلامی، تهران، ایران Zahra Tahmasebi Fard زهرا طهماسبی فرد ztahmasebifard@yahoo.com 100319475328460026069 100319475328460026069 Yes Department of Biology, Roudehen Branch, Islamic Azad University, Roudehen, Iran گروه زیست شناسی، واحد رودهن، دانشگاه آزاد اسلامی، رودهن، ایران