en گیاهان دارویی Pharmaceutical Plants پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span lang="EN-GB" style="font-size:10.0pt"><span style="background:#fafafa"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.2pt">Background & Objective</span></span></span></span></span></i></b><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">:</span></span></i></b><i> </i><span lang="EN-GB" style="font-size:10.0pt"><span style="background:white"><span new="" roman="" style="font-family:" times=""><span style="color:#1c1d1e">Plants are reservoirs of bioactive compounds, which are known to be chemically balanced, effective and least injurious as compared to synthetic medicines. The current resistance and the toxic effects of the available drugs have brought the trend to assess the antileishmanial effect of various plant extracts and their purified compound/s. </span></span></span></span><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Alliums</span></span></i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> are rich sources of steroidal saponins, flavonoids, and sulphuric compounds of which steroidal saponins have recently received more attention due to their important pharmacological activities. <i>Allium austroiranicum </i>is a common edible vegetable in western regions of Iran, especially in &quot;Chaharmahal and Bakhtiari&quot; province, where it is named &ldquo;Lopo&rdquo; and is considerably used as a raw vegetable, flavoring agent, and as a medicinal plant. </span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Materials & Methods:</span></span></span></i></b><i> </i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">The chloroform-methanolic extract was fractionated using MPLC, and the appropriate fractions were then subjected to isolation and purification of the constituents by HPLC. Structure elucidation was done using comprehensive spectroscopic methods including 1D and 2D NMR. Antileishmanial effects of the isolated compound against the promastigotes of <i>Leishmania</i> <i>major</i> were evaluated using MTT method.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Results</span></span></i></b><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">:</span></span></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> Phytochemical investigation of chloroform-methanol extract of the plant resulted in the isolation and identification of a <span style="color:black">Nicotianoside C </span>related steroidal saponin and its chemical structure was determined as (25S)-5&alpha;-Spirostan-1&beta;,3&beta;-diol-3-O-{&alpha;-L-rhamnopyranosyl-(1&rarr;2)-{&alpha;-L-rhamnopyranosyl-(1&rarr;4)}-[&beta;-D-glucopyranoside}. Investigation of <i>in vitro</i> antileishmanial activity of the isolated compound, in 10 and 50 and 100 &mu;g/mL concentrations, exhibited significant leishmanicidal effects against the promastigotes of <i>Leishmania major</i>. </span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></i></b><i> </i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">The results established a valuable basis for further studies about <i>A. austroiranicum</i> and anti-parasitic activity of steroidal saponins.</span></span></span></span></span></div> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:10.0pt"><span style="background:white"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></b></span></span></span><br> &nbsp; Allium austroiranicum, Antileishmania, Saponin, Structure elucidation 162 170 http://jabs.fums.ac.ir/browse.php?a_code=A-10-3014-1&slc_lang=en&sid=1 Zeinab Delazar زینب دل آذر delazar.z@pharm.mui.ac.ir 100319475328460026073 100319475328460026073 No Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Iran گروه فارماکوگنوزی، دانشکده داروسازی و علوم دارویی، دانشگاه علوم پزشکی اصفهان، ایران Mahrouz Ashrafi Rahaghi مهروز اشرفی mahrozashrafi@gmail.com 100319475328460026074 100319475328460026074 No Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Iran گروه فارماکوگنوزی، دانشکده داروسازی و علوم دارویی، دانشگاه علوم پزشکی اصفهان، ایران Masoud Sadeghi Dinani مسعود صادقی دینانی m_sadeghi@pharm.mui.ac.ir 100319475328460026075 100319475328460026075 Yes Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Iran گروه فارماکوگنوزی، دانشکده داروسازی و علوم دارویی، دانشگاه علوم پزشکی اصفهان، ایران