en گیاهان دارویی Pharmaceutical Plants پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Background & Objective:</span></span></i></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> One of the side effects of radiotherapy can be damage to spermatogonial stem cells that &lrm;may lead to spermatogenesis disorders and sterility. Protective effects of curcumin on normal cells &lrm;against radiotherapy side effects have already been shown. In the current study, the protective effects of curcumin &lrm;on the spermatogonial stem cells against gamma radiation were &lrm;evaluated.&lrm;</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Materials & Methods:</span></span></i></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> This study was done on 50 adult rats in 10 experimental groups. Four groups were &lrm;injected 0, 25, 50, or 100mg/kg of curcumin in 1ml olive oil for 15 days intraperitoneally, then exposed to radiation &lrm;at 2 Gy on the next day. Also, four groups were treated like above but without radiation; and two groups as control with and without radiation.&nbsp; The day after radiation, all of the rats were euthanatized, their testes were removed, and they underwent enzymatic digestion to co-culture spermatogonial stem cells. After 12 days, the colonization of spermatogonial stem cells was assessed. &lrm;</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></i></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> There was a significant decrease in the colonization of spermatogonial stem cell proliferation &lrm;in groups that had taken radiation but not curcumin. There was a significant increase &lrm; in the colonization of spermatogonial stem cells in the group which had taken radiation whit maximum curcumin compared with the other irradiated groups and &lrm;was similar to non-irradiated control animals. Colonization of spermatogonial stem cells in non-irradiated animals treated with curcumin had increased compared with control groups.&lrm;</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></i></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> Injection of curcumin can protect spermatogonial stem cells against &lrm;radiation. Thus, curcumin may prevent sterility in men who undergo radiotherapy. &lrm;</span></span></span></span></span><br> &nbsp;</div> Curcumin, Radiation therapy, Radiation protective agents, Spermatogonial stem cells, Sterility 194 202 http://jabs.fums.ac.ir/browse.php?a_code=A-10-2942-1&slc_lang=en&sid=1 Sareh Azarmi ساره آزرمی sareh.azarmi@yahoo.com 100319475328460026090 100319475328460026090 No Faculty of Veterinary Medicine, Tehran University, Tehran, Iran دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران ‎khosro Hoseini ‎pajooh خسرو حسینی پژوه pajooh@irost.ir 0043390560 100319475328460026091 Yes Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran گروه بیوتکنولوژی، سازمان تحقیقات علم و فناوری ایران (IROST)، تهران، ایران Parviz Tajik پرویز تاجیک ptajik@ut.ac.ir 100319475328460026092 100319475328460026092 No Department of Theriogenology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran گروه تریوژنولوژی، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران ‎Marziyeh Lashkari مرضیه لشکری m-‎lashkari@sina.tums.ac.ir 100319475328460026093 100319475328460026093 No Department of Radiotherapy, Tehran University of Medical Sciences, Tehran, Iran گروه رادیوتراپی، دانشگاه علوم پزشکی تهران، تهران، ایران