en ارتباط بین تشکیل بیوفیلم با حضور ژن های متالوبتالاکتامازی و کارباپنمازی در جدایه های بالینی کلبسیلا پنومونیه بیمارستان های شهر قم ميكروبیولوژی Microbiology پژوهشي Research <div style="text-align: justify;"><strong><em>Background & Objectives:</em></strong> <em>Klebsiella pneumoniae</em> is a significant cause of nosocomial infections. This bacterium survives in difficult conditions by forming biofilms in hospital equipment and causes severe infections. On the other hand, the emergence and spread of carbapenem resistance among bacteria and biofilm production is a current health concern. There are controversial findings about the relevance of this issue. This study aimed to evaluate the relationship between biofilm formation and carbapenem resistance among clinical isolates<strong>.<span dir="RTL"></span></strong><br> <strong><em>Materials & Methods</em></strong><strong>:</strong> A total of 160 isolates of <em>Klebsiella pneumoniae</em> were collected. Molecular methods were used to detect resistance genes. Subsequently, the ability to produce biofilms in isolates with resistance genes was assessed. Finally, the correlation of biofilm formation among resistant isolates was calculated using &chi;2 test<strong><span dir="RTL">.</span></strong><br> <strong><em>Results:</em></strong> 79 imipenem-resistant isolates were obtained. 46 isolates (66.66%) containing VIM gene, 36 isolates (52.17%) containing OXA-48 gene,&nbsp; five isolates (7.24%) containing NDM gene,&nbsp; Six isolates (8.69%) containing gene IMP and&nbsp; five isolates (7.24%) also&nbsp; had KPC gene. The results showed a significant correlation between the ability to form biofilms and the presence of carbapenem-resistant genes<strong>.</strong><br> <strong><em>Conclusion:</em></strong> Increased carbapenem resistance in <em>Klebsiella pneumoniae</em> isolates and its association with biofilm formation is severe warning for basic measures to combat this phenomenon.<span dir="RTL"> </span><br> &nbsp;</div> Klebsiella pneumoniae, biofilm, drug resistance, carbapenemase gene, metallobetalactamase gene 3951 3957 http://jabs.fums.ac.ir/browse.php?a_code=A-10-2919-2&slc_lang=en&sid=1 Seyyed Soheil Aghaei سید سهیل آقایی soheilaghaee@yahoo.com 0056868381 100319475328460026402 Yes Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran گروه میکروبیولوژی، واحد قم، دانشگاه آزاد اسلامی، قم، ایران Ali Javadi علی جوادی 0381418138 100319475328460026403 No Department of Medical Science, Qom Branch, Islamic Azad University, Qom, Iran گروه علوم پزشکی، واحد قم، دانشگاه آزاد اسلامی، قم، ایران