en اثر مقایسه‌ای بروملین و ویتامین E بر ناهنجاری‌های اسکلتی ناشی از بیس فنول A در جنین موش صحرایی جنين شناسي Embryology پژوهشي Research <div style="text-align: justify;"><strong><em>Background & Objective:</em></strong> The teratogenic and embryotoxic potential of Bisphenol A (BPA) has been identified in recent years. Bromelain is a natural compound of pineapple that contains different beneficial effects on the fetus. So, this study aimed to investigate the effect of bromelain against BPA-induced skeletal anomalies in the rat fetuses.<br> <strong><em>Materials & Methods:</em></strong> In this experimental study, 36 pregnant Wistar rats were divided into 6 groups including control, BPA (300 mg/kg), Bromelain (40 mg/kg), BPA + Bromelain (10 mg/kg), BPA + Bromelain (40 mg/kg) and BPA + Vitamin E (100 mg/kg). The treatment period was at the 6-15th days of gestation. Fetuses were collected at the 20th day of gestation and after clarification, the skeletal system was stained by Alizarin red and Alcian blue method. Then, skeletal anomalies were evaluated using a stereomicroscope.<br> <strong><em>Results</em></strong><em>:</em> The BPA increased anomalies percentage of cleft palate, spina bifida, non-ossification of the sternum, non-ossification of the last rib, delayed ossification of the forelimb, non-ossification of forepaw, delayed ossification of hindlimb, and non-ossification of the hind paw. Administration of bromelain, as same as vitamin E, reduced the percentage of these anomalies. However, the higher dose of bromelain had a better effect than its lower dose and vitamin E.<br> <strong><em>Conclusion</em></strong><em>:</em> Bromelain is dose-dependent and even better than vitamin E, can reduce skeletal anomalies induced by bisphenol A in the rat fetus.<br> &nbsp;<br> &nbsp;</div> Bisphenol A, Bromelain, Skeletal Anomalies, Fetusl anomalies induced by bisphenol A in the rat fetus 3877 3885 http://jabs.fums.ac.ir/browse.php?a_code=A-10-2813-1&slc_lang=en&sid=1 Kaveh Khazaeel کاوه خزائیل k.khazaeil@scu.ac.ir 100319475328460026369 100319475328460026369 Yes Department of Basic Sciences, Stem Cells and Transgenic Technology Research Center (STTRC), Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran گروه علوم پایه، مرکز تحقیقات فناوری تراریخته و سلول های بنیادی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران. دانشگاه شهید چمران اهواز، اهواز، ایران Mahmood Khaksary-Mahabady محمود خاکساری مهابادی mkhaksary@scu.ac.ir 100319475328460026370 100319475328460026370 No Anatomical Sciences Research Center, Faculty of Basic Sciences, Kashan University of Medical Sciences, Kashan, Iran مرکز تحقیقات علوم تشریحی، دانشکده علوم پایه، دانشگاه علوم پزشکی کاشان، کاشان، ایران Javad Jamshidian جواد جمشیدیان jjamshidian@scu.ac.ir 100319475328460026371 100319475328460026371 No Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran گروه علوم پایه، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران. Narges Zolfaghari نرگس ذوالفقاری na.zolfaghari71@gmail.com 100319475328460026372 100319475328460026372 No Department of Veterinary Medicine, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran گروه دامپزشکی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران