fa بررسی اثر پروبیوتیک‌های اسپوردار بر بیان ژن‌های بیماری‌زای hly، plc و inlA باکتری لیستریا مونوسیتوژنز ميكروبیولوژی Microbiology پژوهشي Research <div style="text-align: justify;"><em><strong>Background & </strong></em><strong>Objectives</strong>: &nbsp;<em>Listeria monocytogenes</em> is known as a foodborne pathogen that causes disease in humans. Also, due to the emergence of drug resistance and the formation of biofilms in the environment, there is a need to use new methods such as probiotics to combat this bacterium. This study aimed to investigate the effect of spore-forming probiotics on <em>hly</em>, <em>plc</em>, <em>inl</em>A virulence genes of <em>Listeria monocytogenes</em><strong>.</strong><br> <strong><em>Materials & Methods</em></strong><em>:</em> In this study, <em>L. monocytogenes</em> serotype 4b strain isolated from human abortion was obtained from Microbial Bank of Iran University of Medical Sciences. <em>Bacillus laterosporus</em> (PTCC 1486) and <em>Bacillus megaterium</em> PTCC1656) were used as probiotic strains. Spore-forming probiotics were co-cultured with <em>L. monocytogenes</em> at different time points. The expression level of virulence genes of <em>L. monocytogenes</em> was assessed by real-time PCR.<span dir="RTL"></span><br> <strong><em>Results:</em></strong> The greatest decrease in expression under the influence of <em>Bacillus latrosporus</em> was related to <em>plc</em>A gene after 24 hours of treatment with 6 folds. <em>Bacillus megatrium</em> had the greatest effect on <em>hly</em> gene, so that at 4, 8 and 24 hours after treatment, its expression decreased by 4, 7 and 5 fold, respectively.<br> <strong><em>Conclusion:</em></strong> The results of the present study showed that both probiotics had an inhibitory effect on the expression of <em>Listeria monocytogenes</em> virulence genes and the inhibitory and therapeutic properties of these probiotics should be considered.<br> &nbsp;</div> Listeria monocytogenes, Spore-forming probiotic, hly, plc,inlA genes 2916 2925 http://jabs.fums.ac.ir/browse.php?a_code=A-10-2736-1&slc_lang=fa&sid=1 Sajad Rajabi سجاد رجبی sajjadrajabi23@yahoo.com 100319475328460024716 100319475328460024716 No Department of Microbiology, Tehran North Branch, Islamic Azad University, Tehran, Iran گروه میکروب‌شناسی، دانشگاه آزاد اسلامی واحد تهران شمال، تهران، ایران Davood Darban داوود دربان davood_darban@yahoo.com 100319475328460024717 100319475328460024717 No Department of Microbiology, Faculty of Medicine, Iran University of Medical Science, Tehran, Iran گروه میکروب‌شناسی، دانشکده پزشکی، دانشگاه علوم پزشکی ایران، تهران، ایران Robabeh Rafie Tabatabaei ربابه رفیعی طباطبائی rrtabatabaei@yahoo.com 100319475328460024718 100319475328460024718 No Department of Microbiology, Tehran North Branch, Islamic Azad University, Tehran, Iran گروه میکروب شناسی، دانشگاه آزاد اسلامی واحد تهران شمال، تهران، ایران Farzaneh Hosseini فرزانه حسینی Farzaneh953@yahoo.com 100319475328460024719 100319475328460024719 Yes Department of Microbiology, Tehran North Branch, Islamic Azad University, Tehran, Iran گروه میکروب شناسی، دانشگاه آزاد اسلامی واحد تهران شمال، تهران، ایران