fa تأثیر عصاره هیدروالکلی میوه گیاه بامیه بر هورمون‌های محور هیپوفیز-بیضه و بیان ژن آرژیناز 2 در موش‌های صحرایی بالغ دیابتی شده با استرپتوزوتوسین گیاهان دارویی Pharmaceutical Plants پژوهشي Research <div style="text-align: justify;"><br> <strong><em><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">Background & Objectives:</span></span></span></em></strong><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;"> Diabetes is the most common metabolic disorder that disrupts the reproductive system. This study aimed to investigate the effect of <em>Abelmoschus esculentus</em> fruit hydroalcoholic extract on pituitary-testicular hormones and the expression of <em>Arginase</em> II gene in streptozotocin-induced diabetic adult rats.</span></span></span><br> <strong><em><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">Material & Methods:</span></span></span></em></strong> <span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">In this experimental study, 36 adult Wistar rats were randomly divided into six groups including the control (untreated), positive control (60 mg/kg streptozotocin), negative control (400 mg/kg <em>Abelmoschus esculentus</em> hydroalcoholic extract), experimental 1, 2 and 3 (first 60 mg/kg streptozotocin and then 100, 200 and 400 mg/kg of <em>Abelmoschus esculentus</em> hydroalcoholic extract, respectively). 28 days later, luteinizing hormone (LH), follicle-stimulating hormone (FSH) and testosterone hormones were measured by ELISA and the Arginase II gene expression was measured by Real-Time PCR. The results were analyzed by one-way analysis of variance and Tukey&rsquo;s <em>post hoc</em> tests.</span></span></span><br> <strong><em><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">Results:</span></span></span></em></strong> <span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">Compared to the control group, the levels of FSH and LH hormones increased significantly and the testosterone level decreased significantly in the positive control group. However, there were no significant changes in hormone levels in the negative control group. In experimental groups, the improvement in FSH, LH and testosterone levels was observed as dose-dependent compared to the control group. The expression level of <em>Arginase</em> II gene in the positive control and experimental 1 groups significantly reduced compared to the control group, but no significant changes were observed in the other groups.</span></span></span><br> <strong><em><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">Conclusion:</span></span></span></em></strong> <span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">The <em>Abelmoschus esculentus</em> fruit hydroalcoholic extract in maximum dose has protective effects on the level of pituitary-testicular hormones and the expression of <em>Arginase</em> II gene in diabetic rats.</span></span></span></div> &nbsp; Diabetes, Abelmoschus esculentus, Testis, Rat, Testosterone 2737 2746 http://jabs.fums.ac.ir/browse.php?a_code=A-10-2651-1&slc_lang=fa&sid=1 Mahya Hosseinpur محیا حسین پور mahya.h.hpr@gmail.com 100319475328460023896 100319475328460023896 No Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran گروه زیست‌شناسی، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران Mehrdad Shariati مهرداد شریعتی mehrdadshariati@kau.ac.ir 100319475328460023897 100319475328460023897 Yes Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran گروه زیست‌شناسی، واحد کازرون، دانشگاه آزاد اسلامی، کازرون، ایران Ebrahim Hosseini ابراهیم حسینی ebrahim.hossini@yahoo.com 100319475328460023898 100319475328460023898 No Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran گروه زیست‌شناسی، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران