fa بررسی ارتباط ژن های ویرولانس flu و csgA با توانایی تشکیل بیوفیلم در جدایه های بالینی باکتری اشرشیاکلی یوروپاتوژن ميكروبیولوژی Microbiology پژوهشي Research <h1 style="text-align: justify;"></h1> <div><strong><em>Background & Objective:</em></strong> There are a variety of virulence factors that allow Uropathogenic <em>E. coli</em> (UPEC) to cause infection in the urinary tract among which biofilm production ability plays the most important role. This study aimed to investigate the possibility of a <em>link between biofilm</em> formation and the <em>flu</em> and <em>csgA</em> virulence genes in this bacterium.<br> <strong><em>Materials & Methods:</em></strong> The 45 UPEC isolates were collected from patients with the urinary tract infection in Rasht and following the biochemical and microbiological standard tests, the presence of <em>flu</em> and <em>csgA</em> virulence genes in confirmed isolates was investigated by polymerase chain reaction (PCR). Antibiotic sensitivity of <em>the isolates w</em>as <em>studied</em> with <em>antibiogram testing</em><em> to determine </em><em>its relationship with biofilm formation ability.</em><br> <strong><em>Result:</em></strong> The highest antibiotic resistance profile was seen against chloramphenicol and tetracycline. Based on PCR test results, 60% and 82% of isolates have <em>flu</em> and <em>csgA</em> virulence genes, respectively and both genes were present in 55.5% of isolates. Four isolates with low, 10 isolates with strong and 11 isolates with moderate biofilm formation abilities were found to have both <em>flu</em> and <em>csgA</em> genes.<span dir="RTL"></span><br> <strong><em>Conclusion:</em></strong> The results showed a significant association between the presence of <em>flu</em> and <em>csgA</em> genes and biofilm production abilities in UPEC strains. Therefore, these genes can be further investigated as a suitable target for therapeutic interventions. In addition, due to the high resistance of these isolates to chloramphenicol and tetracycline antibiotics, these antibiotics are not recommended for the treatment of patients with urinary tract infections.<br> &nbsp;</div> Uropathogenic Escherichia coli, Biofilm, Urinary tract infection, flu, csgA  2775 2785 http://jabs.fums.ac.ir/browse.php?a_code=A-10-2390-1&slc_lang=fa&sid=1 Faezeh Moghadas Kasani فائزه مقدس کاسانی Faezeh.moghadass1993@gmail.com 100319475328460023976 100319475328460023976 No Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran گروه زیست‌شناسی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران Ali Salehzadeh علی صالح زاده salehzadehmb@yahoo.com 100319475328460023977 100319475328460023977 Yes Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran گروه زیست‌شناسی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران Amir Jalali امیر جلالی a-jalali@araku.ac.ir 0069338981 100319475328460023978 No Department of Biology, Faculty of Sciences, Arak University, Arak, Iran گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه اراک، اراک، ایران