fa اثرات ترکیب آلوم و ملاتونین بر پاسخ‌های ایمنی در موش سفید آزمایشگاهی ایمن شده با سالمونلا تیفی موریوم کشته‌شده ايمونولوژي Immunology پژوهشي Research <div style="text-align: justify;"><strong><em>Background & Objectives:</em></strong> Melatonin is one of the immuno-modulator compounds. The purpose of this study was to determine the feasibility of improving immune responses following the use of melatonin in a vaccine made from the killed form of <em>Salmonella typhimurium</em> and alum.<br> <strong><em>Materials & Methods:</em></strong> This experimental study consists of 50 male NMRI mice that were allocated in 5 equal groups, randomly. Each mouse in different groups was subcutaneously treated with killed preparations of bacteria (10⁶ CFU), killed preparations of bacteria with alum, killed preparation bacteria with melatonin (100mg/kg), combined melatonin and alum with killed preparation bacteria, and finally PBS at two-week intervals. One week after the last immunization, one-half of the mice were euthanized and used for immunological evaluations. Other mice in each group were challenged intraperitoneally with the 10⁷CFU live preparation of <em>S.typhimurium</em> in order to evaluate the protective efficacy of vaccine protocols<span dir="RTL">.</span><br> <strong><em>Results:</em></strong> Combined vaccine caused a more favorable survival curve concurrent with a significant increase in the delayed-type hypersensitivity immune response and lymphocyte proliferation after challenging with bacterial antigen compared to other groups (P<0.05). The antibody titers against O and H antigens in the Widal test showed an increase in the alum group more than the melatonin group<span dir="RTL">.</span> The antibody titers showed an increase in the combined group. Nevertheless, this change did not show any significant effect in comparison with the alum group<span dir="RTL">.</span><br> <strong><em>Conclusion:</em></strong> Combined melatonin and alum caused a significant increase in immunogenicity and protective effects against the killed preparation of <em>S. typhimurium.</em><br> &nbsp;</div> Salmonella typhimurium, Vaccine, adjuvant, Alum, Melatonin 2815 2825 http://jabs.fums.ac.ir/browse.php?a_code=A-10-490-4&slc_lang=fa&sid=1 Zahra Jvadi زهرا جوادی 100319475328460023964 100319475328460023964 No Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran گروه میکروبیولوژی، دانشکده دامپزشکی، دانشگاه ارومیه، ایران Seyyed Meysam Abtahi Froushani سید میثم ابطحی فروشانی meysamabtahi@hotmail.com 100319475328460023965 100319475328460023965 Yes Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran گروه میکروبیولوژی، دانشکده دامپزشکی، دانشگاه ارومیه، ایران Melahat Ahmadi ملاحت احمدی 100319475328460023966 100319475328460023966 No Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran گروه میکروبیولوژی، دانشکده دامپزشکی، دانشگاه ارومیه، ایران