fa تمایز سلول‌های بنیادی مزانشیمی ژله‌ی وارتون بند ناف انسان به سلول‌های تولید کننده‌ی انسولین در محیط آزمایشگاهی بيولوژي سلولی-مولكولي Cellular-Molecular Biology پژوهشي Research <p><font face="Times New Roman" size="3">&nbsp;</font></p> <p class="MsoNormal" dir="rtl" style="margin: 0cm 11.85pt 10pt 0cm text-align: justify line-height: normal"><b><span 10pt="" 8.0pt="" b="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-hansi-font-family:="" new="" span="" style="font-family: " times="">دیابت یکی از بیماری‌های مزمن عمده‌ی متابولیک می‌باشد. پیوند جزایر لانگرهانس روشی برای درمان دیابت است ولی به علت مشکلاتی مانند رد سیستم ایمنی و کمبود جزایر اهداء کننده محدود می‌شود. سلول‌های بنیادی مزانشیمی قابلیت تمایز به سلول‌های تولید کننده انسولین را دارا هستند.</span><span 10pt="" 8.0pt="" b="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-fareast-font-family:="" mso-hansi-font-family:="" new="" span="" style="font-family: " times="">تمایز </span><span 10.0pt="" 10pt="" 8.0pt="" 8pt="" b="" dir="rtl" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-fareast-font-family:="" mso-hansi-font-family:="" new="" serif="" span="" style="font-family: " times="">) به سلول‌های بتا، را بررسی می‌کنیم. <!--stripped--><!--stripped--></span></b></p> <p><b><font face="Times New Roman" size="3">&nbsp;</font></b></p> <p class="MsoNormal" dir="rtl" style="margin: 0cm 11.85pt 10pt 0cm text-align: justify line-height: normal"><b><b><span 10pt="" 8.0pt="" b="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-hansi-font-family:="" new="" span="" style="font-family: " times="">نمونه بند ناف انسان بعد از اتمام زایمان (سزارین) از بیمارستان حافظ جمع آوری شد. </span><span 10.0pt="" 8pt="" b="" dir="ltr" font-size:="" mso-bidi-font-family:="" mso-bidi-font-size:="" new="" serif="" style="font-family: " times="">HUMSC_S</span><span 10.0pt="" 10pt="" 20="" 8.0pt="" 8pt="" b="" dir="rtl" dmem-="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-hansi-font-family:="" new="" serif="" span="" style="font-family: " times="">، رتینوئیک اسید، فاکتور رشد اپیدرمی</span><span 10.0pt="" 8pt="" b="" dir="ltr" font-size:="" mso-bidi-font-family:="" mso-bidi-font-size:="" new="" serif="" style="font-family: " times="">(EGF) </span><span 10.0pt="" 10pt="" 8.0pt="" 8pt="" b="" dir="rtl" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-hansi-font-family:="" new="" serif="" span="" style="font-family: " times=""> و آنتی بیوتیک به سلول‌های تولید کننده انسولین (</span><span 10.0pt="" 8pt="" b="" dir="ltr" font-size:="" mso-bidi-font-family:="" mso-bidi-font-size:="" new="" serif="" style="font-family: " times="">Islets</span><span .="" 10.0pt="" 10pt="" 8.0pt="" 8pt="" b="" dir="rtl" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-hansi-font-family:="" new="" serif="" span="" style="font-family: " times=""> برای حضور انسولین و از رونویسی معکوس واکنش زنجیره‌ای پلی مراز </span><span 10.0pt="" 8pt="" b="" dir="ltr" font-size:="" mso-bidi-font-family:="" mso-bidi-font-size:="" new="" serif="" style="font-family: " times="">(RT-PCR)</span><span 10.0pt="" 10pt="" 8.0pt="" 8pt="" b="" dir="rtl" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-hansi-font-family:="" new="" serif="" span="" style="font-family: " times=""> و </span><span 10.0pt="" 8pt="" b="" dir="ltr" font-size:="" mso-bidi-font-family:="" mso-bidi-font-size:="" new="" serif="" style="font-family: " times="">NGN₃</span><span .="" 10pt="" 8.0pt="" b="" class="MsoNormal" dir="rtl" face="Times New Roman" font="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-hansi-font-family:="" new="" p="" size="3" span="" style="font-family: " times="">نتایج: </span></b><span 10.0pt="" 8pt="" b="" dir="ltr" font-size:="" mso-bidi-font-family:="" mso-bidi-font-size:="" new="" serif="" style="font-family: " times="">HUMSC_S</span><span 10.0pt="" 10pt="" 8.0pt="" 8pt="" b="" dir="rtl" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-hansi-font-family:="" new="" serif="" span="" style="font-family: " times=""> تغییر شکل دادند. آزمایشات </span><span 10.0pt="" 8pt="" b="" dir="ltr" font-size:="" mso-bidi-font-family:="" mso-bidi-font-size:="" new="" serif="" style="font-family: " times="">RT-PCR</span><span 10.0pt="" 10pt="" 8.0pt="" 8pt="" b="" dir="rtl" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-hansi-font-family:="" new="" serif="" span="" style="font-family: " times=""> و </span><span 10.0pt="" 8pt="" b="" dir="ltr" font-size:="" mso-bidi-font-family:="" mso-bidi-font-size:="" new="" serif="" style="font-family: " times="">NGN₃</span><span 10.0pt="" 10pt="" 8.0pt="" 8pt="" b="" dir="rtl" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-hansi-font-family:="" new="" serif="" span="" style="font-family: " times=""> <span lang="FA"><font face="B Nazanin" size="2">سلول‌های </font></span></span><span 10.0pt="" 8pt="" b="" dir="ltr" font-size:="" mso-bidi-font-family:="" mso-bidi-font-size:="" new="" serif="" style="font-family: " times="">Islet-like cell</span><span 10pt="" 8.0pt="" b="" class="MsoNormal" dir="rtl" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-hansi-font-family:="" new="" p="" span="" style="font-family: " times="">نتیجه‌گیری: </span></b><span 10.0pt="" 8pt="" b="" dir="ltr" font-size:="" mso-bidi-font-family:="" mso-bidi-font-size:="" new="" serif="" style="font-family: " times="">HUMSC_S</span><span 10.0pt="" 10pt="" 8.0pt="" 8pt="" b="" dir="rtl" font-size:="" islet-like="" lang="FA" mso-ansi-font-size:="" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-hansi-font-family:="" new="" serif="" span="" style="font-family: " times=""> <span lang="FA"><font size="2"><font face="B Nazanin">در محیط آزمایشگاهی است و شاید یک پتانسیل جدیدی برای سلول درمانی در دیابت باشد. <!--stripped--><!--stripped--></font></font></span></span></p> <p><font face="Times New Roman" size="3">&nbsp;</font><b><span 10pt="" 8.0pt="" b="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" minor-latin="" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-fareast-theme-font:="" mso-hansi-font-family:="" new="" style="color: red line-height: 115% font-family: " times="">&nbsp;</span></b></p> <p><font face="Times New Roman" size="3">&nbsp;</font></p> <p class="MsoNormal" style="margin: 0cm 0cm 10pt 11.85pt text-align: justify line-height: normal unicode-bidi: embed direction: ltr"><b><i><span font-size:="" new="" serif="" style="color: black font-family: " times="">Background <span dir="rtl" lang="FA"><font face="Times New Roman" size="2">&</font></span> Objective:</span></i></b><b><span font-size:="" new="" serif="" style="color: black font-family: " times="">&nbsp;</span></b><span font-size:="" new="" serif="" style="color: black font-family: " times="">Diabetes is a major chronic metabolic disease in the world. Islet transplantation is a way to treat diabetes. Unfortunately, this method is restricted due to graft rejection and lack of<font size="2"><font face="Times New Roman"><span style="mso-spacerun: yes">&nbsp;</span>donor islets. Mesenchymal Stem Cells (MSC</font></font>_S) have the ability to differentiate into Insulin-Producing Cells (IPCs). In this study, Human Umbilical Mesenchymal Stem Cells (HUMSC_S) were induced to differentiate into pancreatic </span><span 10pt="" font-size:="" mso-fareast-font-family:="" new="" serif="" style="color: black font-family: " times="">&beta;</span><span font-size:="" new="" serif="" style="color: black font-family: " times="">-like cells.<!--stripped--><!--stripped--></span></p> <p><font face="Times New Roman" size="3">&nbsp;</font></p> <p class="MsoNormal" style="margin: 0cm 0cm 10pt 11.85pt text-align: justify line-height: normal unicode-bidi: embed direction: ltr"><b><i><span font-size:="" new="" serif="" style="color: black font-family: " times="">Materials <span dir="rtl" lang="FA"><font face="Times New Roman" size="2">&</font></span> Methods:</span></i></b><b><span font-size:="" new="" serif="" style="color: black font-family: " times="">&nbsp;</span></b><span font-size:="" new="" serif="" style="color: black font-family: " times="">The samples were collected after cesarean section delivery at Hafez hospital. HUMSC_S were cultured in sterile condition, in three steps for 20 days in DMEM-F₁₂, Retinoic Acid (RA), Epidermal Growth Factor (EGF), exendin-4, Fetal Bovine Serum (FBS), and antibiotic. Then, they were differentiated into IPC. DTZ staining employed for determining the presence of insulin and<span dir="rtl"><font face="Times New Roman" size="2">&nbsp;</font></span>Reveres Transcription- Polymerase Chain Reaction (RT-PCR) was done for identifying of gene expression including insulin, PDX1, and NGN3. The Insulin concentration was also evaluated by Immunoradiometric assay.<span dir="rtl" lang="FA"><!--stripped--><!--stripped--></span></span></p> <p><font face="Times New Roman" size="3">&nbsp;</font></p> <p class="MsoNormal" style="margin: 0cm 0cm 10pt 11.85pt text-align: justify line-height: normal unicode-bidi: embed direction: ltr"><b><i><span font-size:="" new="" serif="" style="color: black font-family: " times="">Results:</span></i></b><b><span font-size:="" new="" serif="" style="color: black font-family: " times="">&nbsp;</span></b><span font-size:="" new="" serif="" style="color: black font-family: " times="">HUMSC_S gradually changed from fibroblast-shaped cells to epithelial-like cells and eventually to IPC under special conditions. RT-PCR experiments revealed that these cells expressed insulin, PDX1, and NGN3 genes.<span dir="rtl"><font face="Times New Roman" size="2">&nbsp;</font></span>The cells became red color when stained with DTZ and the insulin secretion was confirmed.<span dir="rtl" lang="FA"><!--stripped--><!--stripped--></span></span></p> <p><font face="Times New Roman" size="3">&nbsp;</font></p> <p class="MsoNormal" style="margin: 0cm 0cm 10pt 11.85pt text-align: justify line-height: normal unicode-bidi: embed direction: ltr"><b><i><span font-size:="" new="" serif="" style="color: black font-family: " times="">Conclusion:</span></i></b><b><span font-size:="" new="" serif="" style="color: black font-family: " times="">&nbsp;</span></b><span font-size:="" new="" serif="" style="color: black font-family: " times="">HUMSC_Shave the ability to differentiate into islet-like cells in vitro and may be a new potential source for cell transplantation in diabetes treatment.<!--stripped--><!--stripped--></span></p> <p><font face="Times New Roman" size="3">&nbsp;</font><font face="Times New Roman" size="3">&nbsp;</font></p> سلول‌های بنیادی مزانشیمی, ژله‌ی وارتون, تمایز سلول‌ها, انسولین Wharton Jelly, Mesenchymal Stromal Cells, Cell Differentiation, Insulin 69 82 http://jabs.fums.ac.ir/browse.php?a_code=A-10-120-1&slc_lang=fa&sid=1 Masoomeh Nekohi Madoei سیده معصومه نکوهی مدویی masoomnekoei@yahoo.com 100319475328460011472 100319475328460011472 Yes Department of Biology, Islamic Azad University, Arsanjan Branch, Fars, Iran. گروه زیست شناسی، دانشگاه آزاد اسلامی واحد ارسنجان، فارس، ایران. Negar Azarpira نگار آذر پیرا negarazarpira@yahoo.com 100319475328460011473 100319475328460011473 No Organ transplant research center, Namazi hospital, Shiraz University of Medical Sciences, Fars, Iran. مرکز تحقیقات پیوند اعضاء بیمارستان نمازی، دانشکده علوم پزشکی شیراز، فارس، ایران. Ladan Sadeghi لادن صادقی ladansadeghi@yahoo.com 100319475328460011474 100319475328460011474 No Organ transplant research center, Namazi hospital, Shiraz University of Medical Sciences, Fars, Iran. مرکز تحقیقات پیوند اعضاء بیمارستان نمازی، دانشکده علوم پزشکی شیراز، فارس، ایران. Solmaz Kamali Far سولماز کمالی فر 100319475328460011475 100319475328460011475 No Department of Biology, Islamic Azad University, Arsanjan Branch, Fars, Iran. گروه زیست شناسی، دانشگاه آزاد اسلامی واحد ارسنجان، فارس، ایران.