Volume 14, Issue 3 (5-2024)
J Adv Biomed Sci. 2024, 14(3): 210-221 |
Back to browse issues page
1- Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran
2- Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
3- Department of Biotechnology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran ,Tavassoli.am@gmail.com
2- Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
3- Department of Biotechnology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran ,
Abstract: (1967 Views)
Background & Objectives: This study aimed to construct a decellularized mouse spleen scaffold and evaluate its cellular compatibility in vitro using murine bone marrow-derived mesenchymal stem cells (BM-MSCs).
Materials & Methods: A combination of physical, chemical, and enzymatic treatments was employed for mouse spleen decellularization. These included multiple freeze-thaw cycles, the ionic detergent sodium dodecyl sulfate (SDS), and enzymatic trypsin. Histological and scanning electron microscopy analyses were conducted up to 7 days post-culture to assess the impact of decellularization and cellular adaptation to the spleen scaffolds.
Results: Histological studies revealed the attachment and penetration of BM-MSCs into the scaffolds on days 5-7 following cell seeding. Furthermore, cell migration into the scaffold was observed 5 days after the seeding process.
Conclusion: The decellularization approach utilized in this study proved to be effective and biocompatible, supporting the preservation and proliferation of BM-MSCs. These findings indicate its potential for spleen tissue engineering applications.
Materials & Methods: A combination of physical, chemical, and enzymatic treatments was employed for mouse spleen decellularization. These included multiple freeze-thaw cycles, the ionic detergent sodium dodecyl sulfate (SDS), and enzymatic trypsin. Histological and scanning electron microscopy analyses were conducted up to 7 days post-culture to assess the impact of decellularization and cellular adaptation to the spleen scaffolds.
Results: Histological studies revealed the attachment and penetration of BM-MSCs into the scaffolds on days 5-7 following cell seeding. Furthermore, cell migration into the scaffold was observed 5 days after the seeding process.
Conclusion: The decellularization approach utilized in this study proved to be effective and biocompatible, supporting the preservation and proliferation of BM-MSCs. These findings indicate its potential for spleen tissue engineering applications.
Keywords: Decellularization, Scaffold, Spleen tissue, Extracellular matrix, Mesenchymal stem cells, Tissue engineering
Type of Study: Research |
Subject:
Histology
Received: 2024/05/16 | Revised: 2024/12/10 | Accepted: 2024/08/18 | Published: 2024/11/2
Received: 2024/05/16 | Revised: 2024/12/10 | Accepted: 2024/08/18 | Published: 2024/11/2
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |