Background & Objective: The Salmonella origin diseases is wide which could be from gastroenteritis, enteric fever, bacteraemia and focal infection. The infection of salmonella usually comes from the consumption of the contaminated food or water with animal or human excrement. This study aimed to design a Quantitative-LAMP method to real-time detection of Salmonella enterica serovar Typhi (S. Typhi), the causative agent of Typhoid fever.
Materials & Methods: A new LAMP primer set specifically was designed based on ViaB gene and used for detection of S. Typhi. To evaluate the analytical specificity, the genome of some Salmonella-related and non-related bacteria were subjected to the S. Typhi LAMP assay. Also, the analytical sensitivity or limit of detection of the assay was evaluated. Furthermore, in the experiment, turbidity in tubes was assessed by a turbidimeter and then a standard curve was depicted by plotting time threshold values against the log of ViaB gene copy number. With this standard curve, we could use the method to make a quantitative method