en اثرات هم‌افزایی نانوسلولز باکتریایی و محیط کشت حاوی HUVEC بر بهبود زخم سوختگی پاتولوژي Pathology پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#1f497d">Background & Objective:</span></span></span></b>&nbsp;<span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Burn injuries are inherently challenging to manage due to extensive tissue destruction and the substantial edema that ensues. This experimental animal study aimed to evaluate the synergistic effects of bacterial nanocellulose (BC) dressing combined with human umbilical vein endothelial cell-conditioned medium (HUVEC-CM) on the healing process of second-degree burn wounds in rats.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#1f497d">Materials & Methods:</span></span></span></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Fifty male Wistar rats were randomly allocated into five groups: healthy control (no wound), negative control (untreated wounds, sham), BC dressing treatment, HUVEC-CM treatment, and combined BC plus HUVEC-CM treatment (n = 10 per group). A standardized burn wound with a diameter of 10 mm was created on the dorsal surface of each rat. The healing process was assessed macroscopically, using wound contraction percentage, and histopathologically over a 14-day period.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#1f497d">Results:</span></span></span></b>&nbsp;<span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">The combination group demonstrated the smallest wound area by day 3 and exhibited significantly enhanced wound closure on days 7 and 14 (p < 0.05). On day 3, wound closure percentages were as follows: negative control (-1.8 &plusmn; 1.7%), BC group (5.6 &plusmn; 2.1%), HUVEC-CM group (4.9 &plusmn; 2.4%), and combination group (9.1 &plusmn; 6.3%). The greatest degree of wound closure was observed in the combination group on day 14 (71.3 &plusmn; 6.8%). Histological analysis revealed that the combination treatment reduced early necrosis and inflammation, promoted granulation tissue formation and angiogenesis by day 7, and resulted in complete re-epithelialization by day 14.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#1f497d">Conclusion:</span></span></span></b>&nbsp;<span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">The combined application of BC and HUVEC-CM synergistically enhances burn wound healing by mitigating early tissue damage and promoting subsequent regenerative processes. This approach represents a promising bioactive strategy for advanced burn wound management.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#1f497d"></span></span></span></b></span></span></span></div> <br> <span style="font-size:11pt"><span style="background:white"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&nbsp;</span></span><b><span style="font-size:16.0pt"><span style="font-family:"Times New Roman","serif""></span></span></b></span></span></span></span>  Burns, Wound, Healing, Rats, Tissue Engineering 167 178 http://jabs.fums.ac.ir/browse.php?a_code=A-10-3526-1&slc_lang=en&sid=1 Mohammad Valizadeh keshmeshtappeh محمد ولیزاده کشمش تپه dr.valizadeh.m@gmail.com 100319475328460030727 0009-0005-0860-5172 No Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران Rahim Hobbenaghi رحیم حب نقی r.hobbenaghi@urmia.ac.ir 100319475328460030728 100319475328460030728 Yes Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران Rasoul Shahrooz رسول شهروز rasoul_shahrooz@yahoo.com 100319475328460030729 100319475328460030729 No Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran گروه علوم پایه، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران Hassan Malekinejad حسن ملکی نژاد Hassanmalekinejad@yahoo.com 100319475328460030730 100319475328460030730 No Department of Pharmacology and Toxicology, School of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran گروه فارماکولوژی و سم‌شناسی، دانشکده داروسازی، دانشگاه علوم پزشکی ارومیه، ارومیه، ایران ali karimi علی کریمی Ali.karimi.af@gmail.com 100319475328460030731 100319475328460030731 No Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran گروه علوم پایه ، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران Hamed Valizadeh حامد ولیزاده h.valley78@yahoo.com 100319475328460030732 100319475328460030732 No Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran مرکز تحقیقات سل و بیماری‌های ریوی، دانشگاه علوم پزشکی تبریز، تبریز، ایران