en آناتومي Anatomy پژوهشي Research <h3 style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><u><span lang="EN" style="font-size:12.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:#2f5496">Background & Objectives:</span></span></span></u></b> <span style="font-size:12.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif">This study aimed to evaluate the therapeutic potential of <em>Bunium persicum</em> (black cumin) essential oil for preventing ischemia/reperfusion (I/R)-induced pancreatic injury in a rat model.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><u><span lang="EN" style="font-size:12.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:#2f5496">Materials & Methods:</span></span></span></u></b> <span style="font-size:12.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif">The total phenolic and flavonoid contents of the essential oil were determined using the Folin-Ciocalteu and aluminum chloride colorimetric assays, respectively. Antioxidant capacity was assessed using the DPPH radical-scavenging assay, and the chemical composition was analyzed by GC-MS. Pancreatic injury was induced by ischemia followed by reperfusion. Rats received pretreatment once daily for 7 days, administered by oral gavage or intraperitoneal injection, and post-treatment 1 day after the surgical procedure. <em>Bunium persicum</em> essential oil was administered at a dose of 0.1 mL/kg. Sham-operated animals underwent laparotomy and ischemia without treatment. The protective effects were evaluated through histological, biochemical, and molecular assessments.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><u><span lang="EN" style="font-size:12.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:#2f5496">Results:</span></span></span></u></b> <span style="font-size:12.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif">A nonsignificant decrease in the expression of the anti-apoptotic gene <em>Bcl-2</em> was observed in rats with I/R-induced pancreatic injury, a finding that correlated with the observed pathological and biochemical alterations. Our data indicate that 7-day oral pretreatment with <em>Bunium persicum</em> attenuated I/R-induced pancreatic damage. This protective effect may be associated with the antioxidant properties of the essential oil, as indicated by a nonsignificant change in malondialdehyde (MDA) levels and a significant increase in superoxide dismutase (SOD) activity relative to the positive control.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><u><span lang="EN" style="font-size:12.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:#2f5496">Conclusion:</span></span></span></u></b> <span style="font-size:12.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif">Oxidative stress plays a key role in the pathophysiology of I/R-induced pancreatic injury. The underlying protective mechanisms of <em>Bunium persicum</em> appear to be multifactorial. These findings suggest that the antioxidant constituents of <em>Bunium persicum</em> essential oil may help mitigate pancreatic tissue damage, warranting further investigation of its potential therapeutic applications.</span></span><span lang="EN" style="font-size:12.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black"></span></span></span></span></span></span></h3> black cumin, ischemia/ reperfusion injury, pancreatitis, malondialdehyde. 0 0 http://jabs.fums.ac.ir/browse.php?a_code=A-10-249-2&slc_lang=en&sid=1 Mohammad Ebrahim Astaneh محمد ابراهیم آستانه meastaneh@yahoo.com 0000-0001-6956-3467 No Department of Anatomical sciences, School of Medicine, Fasa university of Medical Sciences, Fasa, Iran گروه علوم تشریحی، دانشکده پزشکی، دانشگاه علوم پزشکی فسا، فسا، ایران Majid Naghdi مجید نقدی majidnaghdi@yahoo.com 0000-0002-1785-9458 No 1. Department of Anatomical sciences, School of Medicine, Fasa university of Medical Sciences, Fasa, Iran 2. Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran ۱. گروه علوم تشریحی، دانشکده پزشکی، دانشگاه علوم پزشکی فسا، فسا، ایران ۲. گروه آناتومی، دانشکده پزشکی، دانشگاه علوم پزشکی شیراز، شیراز، ایران Alireza Moulazadeh علیرضا مولازاده immunomoula@gmail.com 0000-0002-6206-5067 No Noncommunicable Disease Research Center, Fasa University of Medical Sciences, Fasa, Iran مرکز تحقیقات بیماری‌های غیرواگیر، دانشگاه علوم پزشکی فسا، فسا، ایران Alireza Tavassoli علیرضا توسلی tavaspath790@yahoo.com 0000-0003-3466-8001 No Department of Pathology, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran گروه آسیب‌شناسی، دانشکده پزشکی، دانشگاه علوم پزشکی فسا، فسا، ایران Zohreh Makoolati زهره ماکولاتی zohreh1438@yahoo.com 0000-0002-5895-0792 Yes Department of Anatomical sciences, School of Medicine, Fasa university of Medical Sciences, Fasa, Iran گروه علوم تشریحی، دانشکده پزشکی، دانشگاه علوم پزشکی فسا، فسا، ایران