en بررسی اثرات بهبود دهندگی کوئرستین بر سمیت قلبی ناشی از کلوتیانیدین در رت سم شناسي Toxicology پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><u><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Background & Objectives:</span></span></span></u></b> <span new="" roman="" style="font-family:" times="">Clothianidin, a widely used neonicotinoid insecticide, has raised environmental and health concerns due to its potential toxicity. Quercetin, a flavonoid abundant in various dietary sources,<b> </b>exhibits potent antioxidant properties and <strong>e</strong>ffectively scavenges reactive oxygen species (ROS). The present study aimed to evaluate the <strong>potential protective effects of quercetin</strong><b> </b>against clothianidin-induced cardiotoxicity in a rat model.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><u><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Materials & Methods:</span></span></span></u></b> <span new="" roman="" style="font-family:" times="">Forty-two rats were randomly assigned to six groups: (1) Normal control group: rats received 0.9% NaCl intraperitoneally (IP); (2) Vehicle control group: rats were administered 5% dimethyl sulfoxide (DMSO) IP daily; (3) Clothianidin group: rats received clothianidin (20 mg/kg/day) every three days for 21 days (seven injections); (4&ndash;6) Treatment groups: rats received clothianidin as in group 3, concurrently with quercetin at doses of 2.5, 5, or 10 mg/kg IP daily for 35 days. Serum enzyme activities of creatine kinase-MB (CK-MB) and lactate dehydrogenase (LDH) were measured. In cardiac tissue, total antioxidant capacity (TAC), malondialdehyde (MDA), and nitrate/nitrite levels were assessed. Histopathological evaluations of cardiac tissue were also conducted.<span style="font-weight:normal"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><u><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Results:</span></span></span></u></b> <span new="" roman="" style="font-family:" times="">Clothianidin administration induced significant cardiac injury, evidenced by decreased TAC and increased levels of MDA, nitrate/nitrite, CK-MB, and LDH. Quercetin at a dose of 10 mg/kg significantly mitigated the cardiotoxic effects of clothianidin, as indicated by reduced CK-MB and LDH levels. Furthermore, quercetin enhanced TAC and reduced MDA and nitrate/nitrite concentrations in cardiac tissue compared to the clothianidin-only group.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><u><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Conclusion:</span></span></span></u></b> <span new="" roman="" style="font-family:" times="">Histopathological and biochemical analyses suggest that quercetin at 10 mg/kg exerts cardioprotective effects against clothianidin-induced toxicity, likely through its antioxidant mechanisms.</span><b><u><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496"></span></span></span></u></b></span></span></span><br> &nbsp;</div> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></span></span> Cardiotoxicity, Clothianidin, Oxidative stress, Quercetin, Rat  158 167 http://jabs.fums.ac.ir/browse.php?a_code=A-10-3459-1&slc_lang=en&sid=1 Sajad Damavandi سجاد دماوندی sajaddamavandy@gmail.com 100319475328460029649 0009-0009-4898-3863 No Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran گروه داخلی و آسیب شناسی بالینی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران Vahid Mohammadi وحید محمدی v.mohammadi@urmia.ac.ir 3673579002 100319475328460029650 Yes Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran گروه داخلی و آسیب شناسی بالینی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران Elham Zadeh-Hashem الهام زاده هاشم e.zadehhashem@urmia.ac.ir 100319475328460029651 100319475328460029651 No Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran گروه علوم پایه، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران