en بررسی تغییرات بیان miR-93-3p و ژنهای NFATc1و NFATc2 در سلولهای تک هسته ای خون محیطی بیماران مبتلا به سرطان پستان بيولوژي سلولی-مولكولي Cellular-Molecular Biology پژوهشي Research <div style="text-align: justify;"><span style="line-height:normal"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Background & Objectives</span></span></span></b><b><span lang="EN-AU" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">:</span></span></span></b> <span lang="EN-AU" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Breast cancer is the most lethal malignancy in women. miRNAs function as epigenetic regulators and contribute to the pathogenesis of breast cancer. Nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1) and 3 (NFATc3), are targeted by microRNA-93 (miR-93). This study aims to evaluate the expression of these genes in peripheral blood mononuclear cells (PBMCs) of healthy women and women with breast cancer.</span></span></span><br> <span style="line-height:normal"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Materials & Methods</span></span></span></b><b><span lang="EN-AU" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">: </span></span></span></b><span lang="EN-AU" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">In this cross-sectional study, blood samples were collected from 20 healthy women and 20 women with early-stage breast cancer. After isolating peripheral blood mononuclear cells (PBMCs), RNA extraction and cDNA synthesis were performed. The expression of the desired genes was then examined by Real-Time Polymerase Chain Reaction (RT-PCR). Statistical analysis was conducted. A p-value less than 0.05 was considered statistically significant, and Student&#39;s t-test was used to evaluate the relative changes in gene expression.</span></span></span><br> <span style="line-height:normal"><b><span lang="EN-AU" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Results:</span></span></span></b> <span lang="EN-AU" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">The results demonstrated that the expression of NFATc1 and NFATc3 genes in peripheral blood mononuclear cells (PBMCs) of breast cancer patients was significantly reduced compared to their expression in healthy individuals. Conversely, the expression of the miR-93-3p gene was significantly lower in healthy women than in breast cancer patients (p < 0.05).</span></span></span><br> <span style="line-height:normal"><b><span lang="EN-AU" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Conclusions:</span></span></span></b><b> </b><span lang="EN-AU" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">This study investigated the expression of miR-93-3p and its downstream targets, the NFATc1 and NFATc3 genes, for the first time in peripheral blood mononuclear cells. The expression levels were shown to be significantly different in patients with breast cancer compared to healthy women.</span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span lang="EN-AU" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Keywords:</span></span></span></b><span lang="EN-AU" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> </span></span></span></span></span></div> Breast Neoplasms, Real-Time Polymerase Chain Reaction, MicroRNAs 56 66 http://jabs.fums.ac.ir/browse.php?a_code=A-10-2357-3&slc_lang=en&sid=1 Mansoreh Dastgir منصوره دستگیر Mansorehdastgir@yahoo.com 100319475328460028427 0009-0008-2040-2732 No Department of Genetics, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran گروه ژنتیک، دانشکده علوم پایه، دانشگاه شهرکرد، شهرکرد، ایران Garshasb Rigi گرشاسب ریگی garshasbbiotech@gmail.com 100319475328460028428 100319475328460028428 Yes Department of Genetics, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran گروه ژنتیک، دانشکده علوم پایه، دانشگاه شهرکرد، شهرکرد، ایران Samira Ghaedmohammadi سمیرا گل محمدی samiraghaed@gmail.com 100319475328460028429 100319475328460028429 No Department of Cellular and Molecular Biology, Estahban Higher Education Center, Shiraz University, Estahban, Iran گروه بیولوژی سلولی و مولکولی، مرکز آموزش عالی استهبان، دانشگاه شیراز، استهبان، ایران Sedigheh Tahmasebi صدیقه طهماسبی Sedighehtahmasebi@yahoo.com 100319475328460028430 100319475328460028430 No Breast Disease Research Center, Shiraz University of Medical Sciences, Shiraz, Iran مرکز تحقیقات بیماری‌های پستان، دانشگاه علوم پزشکی شیراز، شیراز، ایران