en ميكروبیولوژی Microbiology پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span lang="TR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Background & Objectives:</span></span></span></i></b><span lang="TR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> Treating infections due to antibiotic-resistant bacteria is a challenge and researchers are looking for new antimicrobial compounds. Galls are&nbsp;abnormal plant growths caused by biological agents and have active compounds. The present study was designed to examine the antibacterial effects of different extracts of wild rose gall </span></span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">(</span></span></span><span lang="EN-GB" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Rosa canina L.)</span></span></span><span lang="TR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> against some pathogenic Gram-negative bacilli. </span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span lang="TR" new="" roman="" style="font-family:" times=""><span style="color:black">Materials & Methods:</span></span></i></b><span lang="TR" new="" roman="" style="font-family:" times=""><span style="color:black"> Methanol, acetone, and aqueous extracts from galls were prepared using Soxhlet apparatus. <span style="background:white">The antibacterial activity of the extracts was determined by<span style="border:none windowtext 1.0pt; padding:0in"> agar well diffusion method, and the m</span>inimum inhibitory concentration and <span style="border:none windowtext 1.0pt; padding:0in">the m</span>inimum bactericidal concentration were assessed by&nbsp;<span style="border:none windowtext 1.0pt; padding:0in"> the microdilution method</span></span>. <span style="border:none windowtext 1.0pt; background:white; padding:0in">The phytochemical composition </span>of galls was investigated by Gas Chromatography-Mass Spectrometry (GC-MS) method. </span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span lang="TR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Results:</span></span></span></i></b><span lang="TR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> The inhibition zones of 500 mg/ml methanol extract of wild rose gall against Escherichia coli, Pseudomonas aeruginosa, Enterobacter aerogenes, Klebsiella pneumoniae, Acinetobacter bummani, Shigella sonei, and Salmonella typhi were 26.7, 25.0, 25.7, 25.7, 26.7, 23.7 and 18.3, respectively. The antimicrobial activity of the extracts was directly related to their concentration. The extracts were active against all the pathogenic bacteria with average MICs ranging from 15.6 to 31.3&nbsp;mg/ml.&nbsp; The methanol extract of wild rose gall showed the highest bactericidal effect on P. aeruginosa and A. bummani at 62.5 mg/ml, respectively. Moreover, oleic acid, palmitic acid, and octadecenoic acid were composed 36.66%, 14.40%, and 13.21% of total active compounds in wild rose gall. </span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span lang="TR" new="" roman="" style="font-family:" times=""><span style="color:black">Conclusion:</span></span></i></b><span lang="TR" new="" roman="" style="font-family:" times=""><span style="color:black"> All of the wild rose gall extracts showed significant antibacterial activities against Gram-negative bacilli. The antibacterial effects may be related to the high amounts of organic acids in wild rose gall extracts</span></span></span></span></span><br> &nbsp;</div> Anti-bacterial compound, Phytochemicals, Plant extracts, Rosa 13 22 http://jabs.fums.ac.ir/browse.php?a_code=A-10-3212-1&slc_lang=en&sid=1 Atefeh Haghparasti عاطفه حق پرستی atihagh@gmail.com 100319475328460027232 100319475328460027232 No Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran گروه میکروبیولوژی، واحد فلاورجان، دانشگاه آزاد اسلامی، اصفهان، ایران Maryam Mohammadi Sichani مریم محمدی سیچانی Ma.Mohammadi1347@iau.ac.ir 100319475328460027233 100319475328460027233 Yes Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran گروه میکروبیولوژی، واحد فلاورجان، دانشگاه آزاد اسلامی، اصفهان، ایران Majid Tavakoli مجید توکلی majid.tavakoli43mail.com@g 100319475328460027234 100319475328460027234 No Agricultural Research, Education and Extension Organization (AREEO), Lorestan Agricultural & Natural Resources Research Center, Khorramabad, Iran سازمان تحقیقات، آموزش و ترویج کشاورزی (AREEO)، مرکز تحقیقات کشاورزی و منابع طبیعی لرستان، خرم آباد، ایران