en بيولوژي سلولی-مولكولي Cellular-Molecular Biology پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Background & Objectives:</span></span></i></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> The expression of neurotransmitters during cancer progression is one of the factors to consider. This study aimed to evaluate the possible expression changes in dopamine receptors (DRD1 and DRD5), in breast tumor tissue compared to healthy adjacent samples, and the effect of quercetin on the expression of these receptors. </span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Materials & Methods:</span></span></i></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> Expression levels of DRD1 and DRD5 were evaluated in 20 breast tumors and healthy adjacent samples using qPCR. The RNA was extracted from all samples, followed by cDNA synthesis and real-time PCR. <i>In vitro</i> experiment was accomplished on breast cancer cell line, Hs578t. Cells were treated with quercetin and evaluated cell viability by MTT assay. DRD1 and DRD5 gene expression was performed in treatment cells compared to untreated control. Statistical analysis was performed to determine the significance. </span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></i></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> The results showed that the DRD1 and DRD5 were unregulated in breast cancer tissue (p<0.01). For cellular experiment, MTT assays revealed that the quercetin induced a significant decrease in cell viability and proliferation in dose and time-dependent but it was not seen in a normal cell line (HDF). Hs578t cells showed a significant reduction of DRD5 in response to quercetin. DRD1 gene downregulation was indicated significant in 72h treatment.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Conclusions:</span></span></i></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> The effect of quercetin on the expression of genes encoding DRD1 and DRD5 showed that this substance reduced the expression of these two genes in treated Hs578t cells compared to untreated cells in the same cell line.</span></span></span></span></span></div> Dopamine receptor, Breast cancer, Quercetin, Gene expression, Hs578t cell line, HDF cell line 177 186 http://jabs.fums.ac.ir/browse.php?a_code=A-10-2357-2&slc_lang=en&sid=1 Kowsar Kiani Rad خسرو کیانی راد skkianirad@gmail.com 100319475328460026080 100319475328460026080 No Department of Genetics, Faculty of Basic Science, Shahrekord University, Shahrekord, Iran گروه ژنتیک، دانشکده علوم پایه، دانشگاه شهرکرد، شهرکرد، ایران Garshasb Rigi گرشاسب ریگی garshasbbiotech@gmail.com 100319475328460026081 100319475328460026081 Yes Department of Genetics, Faculty of Basic Science, Shahrekord University, Shahrekord, Iran گروه ژنتیک، دانشکده علوم پایه، دانشگاه شهرکرد، شهرکرد، ایران Somayeh Reiisi سمیه رئیسی s.reiisi@sku.ac.ir 100319475328460026082 100319475328460026082 No Department of Genetics, Faculty of Basic Science, Shahrekord University, Shahrekord, Iran گروه ژنتیک، دانشکده علوم پایه، دانشگاه شهرکرد، شهرکرد، ایران