en نانوتکنولوژی پزشکی Nanomedicine پژوهشي Research <div style="text-align: justify;"><span style="font-size:12pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:10.0pt">Background & Objective:</span></i></b>&nbsp;<span style="font-size:10.0pt"> The green synthesis of the silver nanoparticles (AgNPs) is an eco-friendly and straightforward synthesis method. This study aimed to investigate the green synthesis of AgNPs using <i>Nasturtium</i> <i>officinale</i> leaf extract and analysis of their antibacterial activity against some nosocomial pathogens. </span></span></span></span><br> <span style="font-size:12pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:10.0pt">Materials & Methods:</span></i></b><span style="font-size:10.0pt"> The obtained AgNPs were characterized using UV-visible spectroscopy, X-ray diffraction (XRD), and scanning electron microscope (SEM). In addition, the antibacterial activity of synthesized AgNPs and <i>N.</i> <i>officinale</i> leaf extract<b> </b>were performed<b> </b>against five bacterial strains (<i>Staphylococcus</i></span></span></span></span><br> <span style="font-size:12pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><i><span style="font-size:10.0pt">&nbsp;epidermidis, Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa</span></i><span style="font-size:10.0pt">) by agar disk diffusion assay and minimum inhibitory concentration test. </span></span></span></span><br> <span style="font-size:12pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:10.0pt">Results</span></i></b><span style="font-size:10.0pt">: The green synthesized AgNPs had significant antimicrobial activity against all studied bacteria. In addition, greater inhibition activity was observed against <i>E. coli</i> and <i>P. aeruginosa</i> rather than other bacteria.</span></span></span></span><br> <span style="font-size:12pt"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:10.0pt">Conclusion</span></i></b><span style="font-size:10.0pt">: The synthesized AgNPs revealed a special antibacterial effect against selected bacteria, compared to leaf extract of <i>N.</i> <i>officinale</i>. Identifying new antibacterial agents with profound efficacy against antibiotic-resistant opportunistic pathogens is essential. These data provide an eco-friendly and rapid green approach for AgNPs synthesis and the potential of AgNPs<i> </i>for use in drug development against nosocomial infections.</span></span></span></span></div> <span style="font-size:12pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"></span></b></span></span><br> <br> &nbsp; Nosocomial pathogens, Silver nanoparticles, Nasturtium officinale 119 131 http://jabs.fums.ac.ir/browse.php?a_code=A-10-1424-2&slc_lang=en&sid=1 Mahsa Al-Sadat Shajareh مهسا سادات شجره A.mirzaie1364@gmail.com 100319475328460026108 100319475328460026108 No Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran گروه زیست شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران Arezoo Dastpak آرزو دست پاک arezoo.dastpak@iauctb.ac.ir 100319475328460026109 100319475328460026109 Yes Department of Biology, Islamic Azad University, Central Tehran Branch, Tehran, Iran گروه زیست شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران Mozhgan Emtyazjoo مژگان امتیازجو Emtyazjoo.m@yahoo.com 100319475328460026110 100319475328460026110 No Department of Biology, Tehran North Branch, Islamic Azad University, Tehran, Iran گروه زیست شناسی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران Amir Mirzaie امیر میرزائی Amir_mirzaie92@yahoo.com 100319475328460026111 100319475328460026111 No Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran گروه زیست شناسی، واحد پرند، دانشگاه آزاد اسلامی، پرند، ایران