@article{ author = {ahani, narges and Sangtarash, Mohammad Hossein and Houshmand, Massoud and alipoureskandani, maji}, title = {Types of glioma brain tumors and genetic alterations in signaling pathways in them}, abstract ={Types of glioma brain tumors and genetic alterations in signaling pathways in them Background & Objective: Glioma is a common type of primary brain tumor originating in the glial cells that surrounds and supports neurons in the brain. These tumors arise from three different types of cells that are normally found in the brain: astrocytes, oligodendrocytes, and ependymal cells, accordingly types of glioma include: Astrocytomas, Ependymomas, and Oligoastrocytomas. The signaling pathway has been described in systems biology terms as a complex biological network of three steps: (1) an input step in which membrane receptors and their ligands trigger the signal coming from outside the cell; (2) a core system processing step in which protein kinases transmit the signal to the nucleus; (3) an output step in which transcription factors regulate genes that affect various cellular functions. Conclusion: Glioma characteristics are derived from the activation of these pathways, including uncontrolled proliferation, invasion, and angiogenesis. Understanding the genetic alterations that has occurred in these three steps, leading to the formation and progression of glioma tumors, may help improve patient prognosis in order to identify novel treatment targets. In this review, we provide an overview of the most recent developments and current understanding of genetic alterations in these signaling pathways in glioma.    }, Keywords = { glioma, grade, signaling pathway.}, volume = {9}, Number = {1}, pages = {1177-1186}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1574-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1574-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {FoumanAjirloo, parisa and zaree, arezo and ghorbian, saei}, title = {The Roles of Long non-coding RNAs (lncRNA) in Prostate Cancer}, abstract ={Background & Objective: Prostate cancer is a compound condition in which gene expression has altered. Several surveys have revealed that genetic components have been involved in prostate cancer progression. Findings proposed that they can modify a noteworthy portion of disposing of elements, which is associated to the developing prostate cancer in protein coding sequences. The purpose of this research was to indicate that there are many long non-coding RNAs with over 200 nucleotides length found in the human genome, which showed a significant role in the pathogenesis of prostate cancer.  Conclusion: The role of LncRNAs as tumor suppressors or oncogenes has been demonstrated in several types of cancers, including prostate cancer. Recent investigations have disclosed that LncRNAs transcripts play a pivotal role in the tumorigenesis.    }, Keywords = { prostate cancer, long non-coding RNA, LncRNA}, volume = {9}, Number = {1}, pages = {1187-1199}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1805-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1805-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Mohammadi, Kiyanoush and Safaralizadeh, Rez}, title = {A review on miRNAs as new biomarkers for colorectal cancer}, abstract ={Background & Objective: Since colorectal cancer does not often have phenotypic symptoms in the early stages, the study of biomarkers for the diagnosis and prognosis of the tumor is very important. MicroRNAs are one of the most important biomarkers which attract the attention of many researchers due to a variety of reasons, including their non-invasive nature; these molecules are a group of non-coding small RNAs that suppress gene expression by interacting with mRNA at the post-transcriptional level. The objective of the present study is to investigate the potential of microRNAs as a biomarker for colorectal cancer and evaluating their changes at different pathophysiological and molecular levels of cancer. Methods: In this review study, we explored the PubMed, Google Scholar and Scopus databases to investigate the role of microRNAs in colorectal cancer. Conclusion: The discovery of the fact that microRNAs are able to act as oncogene or tumor suppressor has led to the initiation of extensive investigations on cancer, resulting in the identification of various microRNAs involved in carcinogenesis and its progression. Various studies have shown that microRNAs are dysregulated in tissue samples and body fluids of people with colorectal cancer. MicroRNAs in body fluids such as serum, plasma, and fecal specimens are chemically stable, and their diagnosis is easy, so by studying microRNAs in colorectal cancer, the initiation, stages, spread, or mechanisms of the tumor can be predicted and notified.    }, Keywords = { colorectal cancer, biomarker, miRNAs, cancer diagnosis, noncoding RNAs}, volume = {9}, Number = {1}, pages = {1200-1210}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1692-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1692-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {roeintan, abozar}, title = {Computational Design, Molecular Docking Study and Toxicity Prediction of Some Novel Pralidoxime Derivatives as reactivators of acetyl cholinesterase enzyme}, abstract ={Abstract Background & Objective: oximes as Acetylcholinesterase (AChE) reactivators were developed for the treatment of organophosphate compounds (OPCs) intoxication. Oximes also bind to the active site of AChE, simultaneously acting as reversible inhibitors. Organophosphorus compounds (OPCs) such as soman, sarin, or VX react with acetyl cholinesterase irreversibly. In this research, a group of Pralidoxime derivatives with acetyl cholinesterase enzyme reactivator activity was subjected to a docking study, followed by a Toxicity Risk Assessment. Materials & methods: This is a descriptive-analytic study. In order to investigate the mode of the pralidoxime derivatives coupling with enzyme active site, at first the chemical structures of all compounds were designed by ChemBioDraw Ultra14.0 software. Then so as to carry out energy minimization, it transferred into a Hyperchem software. Docking study was performed by Auto Dock Vina program. Then the results were analyzed utilizing Molegro Virtual Docking software. At the final stage, the toxicity risk assessment of compounds was performed by the OSIRIS program. Results: Docking results revealed the hydrogen bond and hydrophobic interactions were involved in the drug-receptor interactions. Among the all studied compounds, the best docking results were related to No. 9 (4-methylbenzyl group- pathic compound) displayed. In fact, this compound had the most negative ΔGbind (-11.14 Kcal/mol) that indicated favorable interactions with the key amino acid residues at active site of acetyl cholinesterase. Conclusion: In conclusion, According to the results of Docking studies and the evaluation of toxicity risk, it can be concluded that combination No. 9 (4-methylbenzyl group- phatic compound) in comparison with pralidoxime reference compound might be considered as a more reactivator of the acetyl cholinesterase enzyme.      }, Keywords = {Keywords: Molecular Docking, Toxicity Test, reactivator, pralidoxime, acetyl cholinesterase}, volume = {9}, Number = {1}, pages = {1211-1219}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1710-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1710-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Kaviyani-delshad, Ghasem and Vesali-akbarpoor, Leila and SamavatiSharif, Mohammad Ali}, title = {Omentin-1 and chemerin\'s response to two different exercise models in overweight men}, abstract ={ Background & Objectives: Omentin-1 and chemerin are adipocytes that are inversely associated with obesity. The purpose of the present study was to compare the effect of eight weeks of Submaximal and High Intensity Interval swimming practice on changes in serum levels of omentin-1 and chemerin in overweight young men. Materials & Methods: in this semi-experimental study among statistical population (110 students), 24 male students (22.5±1.67 years) purposefully with overweight (26.93±0.69)  were divided into two sub-maximal swimming groups (60-67% of maximum heart rate) and High-intensity training swings (80-90% of maximal heart rate).The training protocol included 8 weeks and 3 days per week and each session with 45-60 minutes. It turned out before and 24 hours after exercise, blood 5cc was taken from the subjects. T-test was used to examine the differences between the groups and for inter-group differences, independent T-test with a significance level of p<0.05 was used.  Results: Both submaximal swimming training (p=0.001) and high intensity interval (p=0.01) resulted in an increase in the levels of omentin-1 and the serum chemerin level in both submaximal swimming (p=0.005) and high intensity interval (p=0.02) increased significantly. These results were associated with weight loss, body mass index, waist-hip ratio, fat percentage and aerobic capacity increase in both groups (p<0.05). Conclusion: Therefore, it can be said that eight weeks of submaximal swimming and high intensity interval, lead to an increase in serum levels of omentin-1 and a decrease in chemerin and obesity-related factors.      }, Keywords = { Submaximal swimming, High intensity interval swimming, Omentin-1, Chemerin, Overweight men }, volume = {9}, Number = {1}, pages = {1220-1229}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1787-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1787-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {bahmani, javad and gholampoor, yousef}, title = {Determination of Radon Gas Concentration in Fasa Residential Houses and the effects of inhalation on the incidence of lung cancer}, abstract ={Background & Objective: The present research project is based on the common ground in nuclear and medicine science. The purpose is to determine the concentration of radon gas in residential homes in Fasa in 1395 for investigating the effects of its inhalation on the incidence of lung cancer in residents of the home. Material & Methods: Among the residential houses, 10 houses were selected in different parts of the city and 20 samples of radon gas infiltration system were installed in these houses in 1395. In this research, using electrochemical stew method, the detectors were scanned by optical scanners and then images of alpha tracks were prepared in the form of image grey scale. After that, with the help of image processing software and using the passive dosimeter method, the mean concentration of radon was determined during a given period of time. Results: The results of counting and measuring the concentration of radon gas in 20 samples collected in two first and second six-month periods of residential buildings were investigated and evaluated. The results showed that radon gas concentrations in Fasa during the first and second six months of 1395 fluctuated from 141 to 417 Becquerel per cubic meter. Conclusion: In both period north-west parts of the Fasa, concentrations above 150 Becquerel per cubic meter was found to be higher than standard concentrations in residential homes, and residents in these areas were at risk of lung cancer. At the end of the article, there are some strategies to reduce the concentration of radon in homes.  }, Keywords = { Radon, Lung Cancer,Alpha, Radiation}, volume = {9}, Number = {1}, pages = {1230-1239}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1599-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1599-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Dehghaniyan, Marzie and Rezaeian, Abbasali}, title = {The Study of LasI and AlgD genes frequency and Multidrug Resistance (MDR) in Pseudomonads isolated from infectious samples of Fasa medical centers}, abstract ={Background and Objective: Pseudomonas aeruginosa is one of the most important pathogenic bacteria causing infectious diseases that include resistance to many antibiotics. In addition, existence of LasIand AlgD gene contributes to pathogenicity of this bacterium. This study is aimed to investigate the frequency of mentioned genes, ‌antibiotic resistance and a significant relationship between them. Materials and Methods: This descriptive cross-sectional study was performed on 91 isolated Pseudomonas from Fasa medical centers. After completing biochemical and microbiological tests, all strains were evaluated by the PCR method in terms of LasIand AlgD genes existence, and antibiotic susceptibility test was performed by disk diffusion and MIC methods. Statistical analysis was performed using SPSS 20 software. Results: Among 91 isolated samples, (59.34%) 54 samples of Pseudomonas aeruginosa and (40.66%) 37 samples of other species were detected. The results of drug resistance pattern determination for both groups showed that highest resistance to Cefepime has been established. 40 isolated Pseudomonas aeruginosa and 30 isolated samples of other species showed a MIC test relative to Cefepime. All isolated samples had AlgD gene. Conclusion: The results of this study revealed high resistance to different antibiotics among Pseudomonas aeruginosa strains and other strains. Quorum sensing genes and alginate generator genes with antibiotic resistance genes can increase the severity of involvement in this group of bacteria.    }, Keywords = {Pseudomonas aeruginosa, AlgD, LasI}, volume = {9}, Number = {1}, pages = {1240-1247}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1670-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1670-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Pouraman, Mohammad and kianifard, davoud and akbari, ghasem}, title = {The Study of Histomorphometry and Histochemistry of Liver in Adult Male and Female Rats Following Short-term and Long-term Administration of Methylphenidate}, abstract ={Background & Objective: Methylphenidate is one of the most common medications used for treatment of attention-deficit/hyperactivity disorder (ADHD). The administration of methylphenidate, through blocking of dopamine receptors and increment of extracellular dopamine levels, leads to escalation in central nervous system activity. Numerous studies investigated the effect of methylphenidate on body organs however; there is no comparative and time dependent study about the effects of this agent on the liver tissue in male and female rats. Material & Methods: Adult male and female rats were treated with methylphenidate (10 mg/kg) for two and eight weeks. The blood level of hepatic enzymes (AST, ALT, ALP) was assessed and the liver tissue samples were prepared for morphometric studies. Microscopic studies were made by digital camera. Histochemical studies were done with Oil Red O staining method. Results: Blood levels of hepatic enzymes were increased (especially in females) in MPH treated groups compared to control rats. Histomorphometric results revealed that the evaluated indices were reduced by following the administration of methylphenidate in male and female rats compared to control group. These reductions were noticeable in long term administration and in female rats. In histochemical investigations, there were no significant differences observed between experimental groups. Conclusion: The results of this study showed that, long term administration of methylphenidate could induce some alterations in hepatic enzymes and microscopic structure of the liver of rats and in this way it seems that female rats were more susceptible in comparison to male rats which can be a reflection of the greater impact of females from the side effects of methylphenidate.  }, Keywords = {Rat, Methylphenidate, Liver}, volume = {9}, Number = {1}, pages = {1248-1256}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1625-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1625-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Sepehri, Saghi and Adhami, Vahe}, title = {Molecular docking study of anti-viral FDA-approved drugs as novel entry and replication Ebola viral inhibitors}, abstract ={Background & Objective: Because of the reported high ability of virulence and the lack of appropriate drug of Ebola virus during the last decades, many investigations have been accomplished regarding discovery and the introduction of anti-Ebola drugs. The aim of this research was the bioinformatical study of entry and replication of Ebola viral inhibition by drug repurposing. Materials & Methods: It is a descriptive-analytic study. In order to investigate the mode of interaction of the compounds with GP and VP40 binding sites, the chemical structures of all compounds were designed using ChemDraw program, then were transferred into Hyperchem software for energy minimization. Molecular docking simulation was accomplished using AutoDock 4.2 program. Results: Docking results revealed the hydrophobic, hydrogen bond, π-π and π-cation contacts were involved in the drug-protein interactions. Among all the studied drugs, the best docking results were related to Amodiaquine and Diphenoxylate drugs displayed. Actually, this compounds had the most negative ΔGbinding that indicated suitable modes and favorable interactions with the amino acid residues at the binding site of GP and VP40. The weakest docking results were exhibited for Dirithromycin and Erythromycin drugs due to the high hydrophilic character of them. In general, the presence of hydrophobic portions, tertiary amines, and optimal hydrogen bonds increases the strength of anti-Ebola medications. Conclusion: According to the results of the molecular docking, the entire FDA-approved drugs revealed a good inhibition effect on entry and replication Ebola viral  }, Keywords = { Docking, Ebola viral, Drug repositioning, VP40, GP}, volume = {9}, Number = {1}, pages = {1257-1266}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1687-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1687-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Mavaddatiyan, Laleh and Khezri, Shiva and AbtahiFroushani, Meysam}, title = {Curcumin ameliorates oxidative stress in animal models of multiple sclerosis}, abstract ={Background & Objectives: The role of oxidative stress in the pathogenesis of Multiple Sclerosis (MS) has been mentioned in previous studies. Curcumin is an active ingredient in Curcuma longa and has anti-inflammatory properties. The aim of this study was to investigate the effects of curcumin on reducing the oxidative stress in the animal model of MS. Materials & Methods: In this study, experimental autoimmune encephalomyelitis (EAE) as a MS model was induced by immunizing of Wistar rats (n=12) with homogenized spinal cord of guinea pig and Freund's complete adjuvant. Then, the immunized animals were divided in two equal groups. Treatment was curcumin (100 mg/kg daily) was started from the day 12 after the immunization when the rats showed the first symptoms of neurologic disability and continued until the day 24 when the rats were sacrificed. Simultaneously, the control group received the distilled water. Treatment was continued until 24 days after the immunization. Results: The consumption of curcumin in the EAE-treated rats caused a significant decrease in nitric oxide level and activity of serum myeloperoxidase enzyme activity (p<0. 05). At the same time, the malondialdehyde level in the brain tissue decreased (p<0. 05). However, no significant change in the total antioxidant capacity of the serum (p<0.05) was observed. Moreover, the serum uric acid level increased in rats after curcumin treatment and reached the level of healthy rats (p<0. 05). Conclusion: Using a 100 mg/kg curcumin in the rats with EAE cause to reduce the harmful effects of oxidative damage and can be effective in MS treatment.    }, Keywords = { Multiple Sclerosis, Experimental Autoimmune Encephalomyelitis, Curcumin, Oxidative stress}, volume = {9}, Number = {1}, pages = {1267-1276}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1614-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1614-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Akbarzade, Zahra and Karimi, Roya and Moameri, Hossei}, title = {Investigating The Relationship between the Consumption of Maternal Nutrition Supplements during Pregnancy and Anthropometric Indices in the Birth of a Newborn in Southern Iran (Case study: Bandar Abbas)}, abstract ={Background & Objectives: inappropriate diet and the lack of necessary nutrition receiving during pregnancy, effects on maternal and neonatal health. This study carried out to investigate the effect of maternal supplements during pregnancy on growth indices at birth. Material & Methods: This cohort study was carried out on part of Bandar Abbas Cohort study data. The main exposure in this study was the consumption of dietary supplements during pregnancy and the dependent variable was the growth indices of newborns. The adjusted relative risk index using a Modified Poisson Regression model was applied. All analysis were performed using the STATA software. Results: 196 of participants remained in the study. The age range of mothers was from 16 to 42 years old and the mean of that was 27.28 (± 5.62). 81, 84 and 149 of mothers had taken an iron supplement, multivitamins, and vitamin D irregularly respectively. 12.76%, 8.68%,18.88% of the newborns, had abnormal weight, height, and head circumference at birth respectively. The relative risk of low birth weight and consumption of multivitamin 2.65 was achieved. Also, the relative risks between low birth height and iron and multivitamin supplements were 3.54 and 4.56 respectively. Conclusion: There was a relationship between irregular consumption of iron and multivitamins during pregnancy with weight and height at birth, but there was no relationship between the consumption of nutritional supplements during pregnancy and the head circumference. Mothers' nutritional supplementation during pregnancy requires more attention.    }, Keywords = {Anthropometric Indices, Birth Weight, Length at birth, Head Circumference, Dietary Supplements.}, volume = {9}, Number = {1}, pages = {1277-1288}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1802-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1802-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Khodadadi, iraj and Shafiee, gholamrez}, title = {The Evaluation of the effects of cytotoxic, anti-proliferative and anti-migrative effects of the thymoquinone on PC3 prostate cancer cell line}, abstract ={Background & Objective: Herbal medicine and their composition are widely used for different therapeutic purposes. Therefore, this study was designed to evaluate of the anti-proliferation and anti-migration effects of Thymoquinone (TQ), an active part of Nigella sativa, on PC3 prostate cancer cells. Materials & Methods: In this experimental study, PC3 prostate cancer cells were treated with 0, 10, 30, 50, 70 and 90 µM concentrations of thymoquinone for 12, 24 and 48 hours and cell viability was determined by MTT assay. IC50 concentration of thymoquinone was obtained as 40 µM concentration of thymoquinone and 24h incubation time. PC3 cell migration was studied using the cell migration assay. Colony formation assay was used to determine cell proliferation and Gimsa staining was performed to observe morphological changes of the cells. Results: The IC50 of Thymoquinone was found in concentration of 40 µM at 24h treatment. Thymoquinone significantly (p<0.05) inhibited cell growth and proliferation as well as cell migration. Treatment of PC3 cells with 40 µM thymoquinone showed significant changes in nucleus and cytoplasm of the cells which are representative of apoptosis and cell death. Conclusion: Thymoquinone has significant anti-proliferative and anti-migrative effects on prostate PC3 cells and therefore may be used as a complementary agent for prevention of prostate cancer.    }, Keywords = { Prostatic neoplasms, Thymoquinone, Migration, Colony formation}, volume = {9}, Number = {1}, pages = {1289-1300}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1630-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1630-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {moradikelardeh, parisa and taheri, mehrdad and zakeri, habib}, title = {The Relationship between chronic low back pain and sleep pattern (using data derived from the Persian cohort; Fasa(}, abstract ={Background & objective: Chronic low back pain is one of the most common disorders associated with chronic pain that is correlated with a wide range of psychological issue such as problems in sleep pattern. The purpose of this study was to investigate the relationship between sleep pattern and chronic low back pain. Materials & Methods: The research method was cross-sectional- descriptive–analytic and the statistical populations were residents of Sheshdeh of Fars province. Among them 1,366 people participated in the study by convenience sampling method. The instrument included a questionnaire of Persian cohort, Oswestry inventory and McGill Questionnaire. Data were analyzed using SPSS . V-24 software. Results: 90.9% of the subjects were male and 39.09% were female. The results showed that there is a reverse relationship between the LBP، pattern and the quality of sleep, and with the increase in pain, the amount of sleep decreases.  Conclusion: results of this study indicate that the quality of sleep should be considered in treatment of patients with chronic pain, especially those with chronic LBP.  }, Keywords = {chronic low back pain, sleep pattern, Persian cohort}, volume = {9}, Number = {1}, pages = {1301-1305}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1856-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1856-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {asadi, sara and sayadi, mehran and Khalighain, sahar and Hashemi, Sima and Allahverdi, Ghader}, title = {The study of the frequency of dyes used in food products delivered at Fasa restaurants using Thin Layer Chromatography and spectrophotometric methods in 1397}, abstract ={Background & Objective: Today, in order to maintain or improve the appearance, composition, flavor and nutritional value for food, compounds called additive are added, that they lead to complications in the human body, which the colors are only one of these substances. The aim of this study was to determine the frequency of consuming colors in saffron foods in Fasa in 1397 by thin layer chromatography and spectrophotometry methods. Materials & Methods: This is a type of Experimental study that was conducted by a cross-sectional study in Fasa. A total of 64 samples of food were collected from restaurants and after color extraction with Hydrochloric acid and purification, the color analysis was performed using thin-layer chromatography and spectrophotometry. The Data were analyzed using Excel software. Results: Out of 64 samples, 36 samples (56.2%) of natural color and 28 samples (43.8%) of artificial color were used. Of this amount, artificial color (57.1%) was non‐permitted artificial color (16 samples) and 42.9% of permitted artificial color (12 samples). Also, 75% of the natural colors used were saffron type. Conclusion: Considering the high level of artificial colors found in the samples of this study, preventive measures are essential because the high consumption of non-edible and edible color in foods and their adverse effects on health, raising the level of awareness of manufacturers and consumers about the complications, the amount of consumption or lack of use of these substances is necessary.    }, Keywords = { Food color, Artificial color, Saffron, Thin Layer Chromatography, Spectrophotometry}, volume = {9}, Number = {1}, pages = {1306-1313}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1778-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1778-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {HasanzadehKhanmiri, Hamid Reza and Shahrooz, Rasoul and Hasanzadeh, Shapour and Najafi, Golam Rez}, title = {Evaluation of the Protective Effect of Crocin on the Development of Embryos from In Vitro Fertilization (IVF) in Busulphan-treated Mice}, abstract ={Background & Objective: Busulfan (BSF) besides the therapeutic effects, performs oxidative stress and decreasing fertilizing capacity. Therefore, the present study was aimed to evaluate the protective effects of Crocin, on in vitro fertilization (IVF) process in busolfan treated mice. Materials & Methods: In this study eighteen mature female mice (25g), were divided into 6 groups and treated for 21 days. The control group received alone resolvent of busulfan (0.1 ml) intraperitoneally (IP) single dose, and sham control group received BSF alone (10mg/kg, IP/single dose) and experimental groups 1, 2, 3 received BSF(10mg/kg/single dose) with Crocin (100, 200, 400mg/kg/day, IP). Positive control group received alone Crocin (400mg/kg, IP/day). At the end of the treatment period, animals were euthanized and after performing the IVF, early embryo development was evaluated. The obtained data were compared between all groups and analyzed using one-way ANOVA, followed by Bonferroni post-hoc test. A P-value <0.05 was considered significant. Results: Results showed that, the administration of crocin along with busulfan increased significantly oocyte quality, fertilization rate, pre-implantation embryonic development and quality of embryo in comparison to group that received busulfan (P<0.05). In addition, in the group that received crocin any toxicity has not been observed. Conclusion: The present study indicated that crocin can protect female fertility potential against busulfan induced damages.    }, Keywords = { Busulfan, Ovary, Crocin, Mice, In Vitro Fertilization}, volume = {9}, Number = {1}, pages = {1314-1323}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1740-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1740-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {arjomandilari, mostafa and parhoodeh, saeed and rohanisarvestani, ali and alahverdi, ghader}, title = {Fabrication of High Frequency Electromagnetic Waves Absorber Sheets by Use of Iron Oxide Nanostructures}, abstract ={ Background & Objective: The necessity of using wireless communications and the use of electromagnetic waves in today's world is obvious to everyone. Using of these equipment and development of them, although have many benefits but causes some anxiety on the way in which they influence on environment. One of the most effective ways of decreasing the unwanted effects of these waves is using of the absorber coatings of these waves. Materials & Methods: First, iron chloride powder was added to the aqueous solution of poly vinyl alcohol. Then, aqueous solution of sodium hydroxide was added to it. Subsequently, the iron oxide nanostructures that appeared in the bottom of the dish as sediment were collected by using filter paper and then washed several times with deionised water and ethanol. From the synthesized materials, sheets of thickness of 0.5 and 1 mm were made. Results: X-ray diffraction results proved the synthesis of iron oxide (Fe3O4). The analysis also showed that the synthesized nanostructures have a cubic structure and the average size of crystallites is 12 nm. The electron microscopy images showed that the nanostructures have a sheet-like shapes and their thickness are about 30 nm. The results of the absorption of electromagnetic waves in the range of 8 to 18 GHz showed that iron oxide nanostructures can absorb microwave waves in this range, which absorption increases in greater thicknesses. Conclusion: Coatings containing iron oxide nanostructures can act as microwave absorber and they could have an effective role in eliminating or reducing the unwanted effects of electromagnetic waves.  }, Keywords = {Waves, Microwave, Absorber, Iron Oxide, Nanostructures, Nanotechnology, Biological effects}, volume = {9}, Number = {1}, pages = {1324-1334}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1994-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1994-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Hoseini, Zahra and Behpour, Nasser and Hoseini, Rastegar}, title = {The Effect of Aerobic Training and Vitamin D Supplementation on Blood Pressure in Elderly Women with Nonalcoholic Fatty Liver and Vitamin D Deficiency}, abstract ={Background & Objective: The aim of this study was to investigate the effects of aerobic training (AT) and vitamin D (Vit D) supplementation on cardiovascular variables in elderly women with nonalcoholic fatty liver disease (NAFLD) and vitamin D deficiency. Materials & Methods: A total of forty women (60-65 years) with NAFLD (second or third grade) and vitamin D deficiency were recruited for the study. Participants were randomly assigned to four AT+Vit D, AT, Vit D, and control (C) groups and were allocated to receive either vitamin D (∼50000 IU • week for eight weeks) or placebo. The aerobic training protocol consisted of 40-60 minutes of aerobic training at 60% - 75% of HRmax, 3 times a week for eight weeks. Anthropometric indices along with systolic blood pressure (SBP), diastolic blood pressure (DBP), and heart rate (HR) were measured and mean of arterial pressure (MAP) was calculated. Results: AT+Vit D, Vit D and AT groups demonstrated a significant decrease in SBP, DBP, and HR, (P < 0.05) while, C group showed a significant increase in the mentioned variables (P < 0.05). Also after 8 weeks, AT + Vit D and AT groups showed significant decrease in the mean of MAP (P = 0.001; P = 0.018). Compared to the control group, significant differences in the SBP, DBP, and HR were observed in all groups. The mean SBP was significantly lower in AT+Vit D group comparing with AT, Vit D, and C groups. Also, no significant differences were observed between AT and Vit D groups in all variables. Conclusion: AT developed significant improvement in cardiovascular variables indicating SBP, DBP, MAP, and HR in elderly women with NAFLD and vitamin D deficiency. Meanwhile, AT combined with Vit D decreased SBP more significantly that might be incorporated in the management programs of the patients suffering from NAFLD in order to augment improvement in their blood pressure.    }, Keywords = {Exercise, Vitamin D, Blood Pressure, NAFLD}, volume = {9}, Number = {2}, pages = {1335-1345}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-2025-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-2025-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Parhoodeh, Saeed and Allahverdi, Ghader}, title = {A Review of Microfluidic Chips Applications in Biological Diagnosis}, abstract ={Recent advances in science and technology caused the Revolution in many different fields of science and Industry. The term lab on a chip or performing difficult analyses only in a short time and in a small space is an expression which becomes very common in recent years. Today, the things which were wishes in the past, are becoming real and inserted in the real life of humankind. In this article, it is tried to study and discuss a special kind of lab on a chip technology, which is called microfluidic (or microchannel). Although this is a vast technology, and it was inserted in many branches of Science and Industry, but here, we have discussed only the applications of this technology in medical and biological diagnostic fields. In this article, we have especially surveyed the devices which were made based on this technology for DNA analysis, devices for detection based on separation, devices for cell sorting and handling and devices for protein-based applications.  }, Keywords = {Lab on a chip, Microfluidic, Microchannel, Nanotechnology, Biology}, volume = {9}, Number = {2}, pages = {1346-1356}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1558-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1558-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {tavalaee, marzieh and ghorbani, razieh and Nasr-Esfahani, mohammad hossei}, title = {The Role of Sperm Protamine in Pathogenesis of Male Infertility}, abstract ={Background & Objective: Approximately 40-50% of infertility is due to male factor infertility that abnormal sperm chromatin structure as a major cause of infertility has been suggested. Protamines are the major components of sperm chromatin and play a central role in the correct chromatin packaging. Several studies have shown that the protamine deficiency in sperm is associated with low sperm quality and infertility. Considering the importance of protamine infertility, the purpose of this review article was to study the protamine content and it’s biological significance in male fertility. Materials & Methods: Published papers by researchers in the Google Scholar, Science Direct and PubMed databases were collected from 1874 to 2018, and the best and most efficient papers were considered for this study. Results: Several studies have shown that there is a significant reverse relationship between protamine deficiency in sperm with fertilization and pregnancy rate following assisted reproduction techniques and central cause of protamine deficiency in sperm is due to the increase of oxidative stress which consequently causes to DNA damage. Conclusion: Treatment with antioxidants can reduce oxidative stress in infertile men, and improve sperm parameters, sperm protamine content, and DNA integrity.    }, Keywords = { Protamine, Male Infertility, spermatogenesis}, volume = {9}, Number = {2}, pages = {1357-1367}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1578-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1578-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Hosseinpouri, Arghavan and Mohammadi, Mahdi and Obeidi, Narges}, title = {Fucoidan, Multifunctional Polysaccharide}, abstract ={Background & Objective: Marine algae contain bioactive compounds that produce a variety of metabolites. These metabolites have diverse biological activities. Fucoidan is a polysaccharide of sulfates produced by algae, and its biological activity has been studied extensively. Fucoidan is a sulfate-rich poly-saccharide containing a variety of compounds including, galactose, zylose, glucuronic acid and fucose. Materials & Methods: Fucoidan has therapeutic properties that increase their healing properties with their degree of sulfation. Anticancer, antiviral, antibacterial, antifungal, anti-inflammatory and adjuvant properties in many species of algae have been proven to have an effect on cell proliferation and cellular regimens and the regulation of various metabolic pathways. Results: This polysaccharide has beneficial effects on human health and therefore is used in cosmetics, food and pharmaceutical industries. Non Toxicity is an important feature of Fucoidan, which is used as a confident structural compound that is a product of safety for consumption. Conclusion: Today, a variety of Fucoidan compositions have been made; a variety of beverage, tablets, and capsules are now available on the market. It is hoped that more complete studies and clinical trials will be carried out for better utilization of this combination in the prevention, diagnosis, and treatment.    }, Keywords = {Fucoidan, Brown Algae, Cancer, Polysaccharide, seaweed}, volume = {9}, Number = {2}, pages = {1368-1383}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1773-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1773-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {RajabiPour, Mahdi and Fardid, Rez}, title = {In Vivo Mechanisms of Radioadaptive Response}, abstract ={Background & Objectives: Radioadaptive response (RAR) describes a phenomenon in which small priming doses of ionizing radiation (IR) reduce detrimental effects of subsequent higher doses. Since IR-induced carcinogenesis is a main concern in the low-dose radiation risk assessmen, the aim of this study was to investigate the RAR with the end points of carcinogenesis and the related genomic damages and evaluation of the effective in-vivo mechanisms in this phenomenon. Materials & Methods: The present review article was performed by using the research and review articles indexed in Pubmed, Google scholar, Science direct. In this review article, some resent studies related to RAR with end points of carcinogenesis in different species of mice and human lymphocytes has been investigated. Additionally, in the present review article, the role of important in vivo mechanisms involved in adaptive response, namely DNA repair, bystander effect and endocrine system hormones such as glucocorticoids has been investigated. Results: These studies, often revealed efficient induction of RAR by chronic or repeated low-dose priming irradiation. Conclusion: Current radiation protection regulations do not include RAR because of the large variability in expression among individuals and uncertainties of the mechanism. However, in the future, RAR should be regarded as an indispensable factor for estimation and control of individual IR sensitivity.  }, Keywords = {adaptive response, Low-dose Radiation, Ionizing Radiation, DNA Repair, Bystander effect, Cancer}, volume = {9}, Number = {2}, pages = {1384-1398}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1717-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1717-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Hosseini, Mahshid and Keshavarz, Mojtaba and Vessal, Mahmoo}, title = {The Contribution of S100B Suppression by Arundic Acid to the Inhibition of the Glio-Toxicity Induced by Beta-Amyloid in an Astrocytes Culture}, abstract ={Background & Objective: It has been shown that glial activation has important role in the pathophysiology of Alzheimer’s disease. S100B is an astrocyte specific factor with deleterious effects on the neuronal and non-neuronal cells in the central nervous system. Arundic acid is an agent that inhibits the secretion and production of S100B in astrocytes. Therefore, we aimed to evaluate the contribution of S100B in the cyto-protective effects of Arundic acid against beta-amyloid in 1321N1 astrocyte cell culture. Materials & Methods: Human astrocyte cells (1321N1) were treated with beta-amyloid (200 μM) and / or Arundic acid (50 μM) for 24 hours. Cell viability was measured using the MTT (3, 4, 5-dimethylthiazole-2, 5-diphenyl tetrazolium bromide) method. The S100B protein level was measured by the ELISA method. Results: Beta-amyloid treatment reduced cell survival compared to the control-treated groups. In contrast, the addition of Arundic acid to beta-amyloid suppressed the beta-amyloid-induced cell death. Beta-amyloid also increased the S100B protein level. However, Arundic acid prevented the rise of S100B protein level induced by beta-amyloid. Conclusion: The reduction of S100B protein secretion may be involved in the protective effects of Arundic acid against the beta-amyloid induced Glio-toxicity in the astrocyte culture.    }, Keywords = { Alzheimer Disease, Amyloid beta-Peptides, S-100 Calcium Binding Protein beta Subunit, Arundic acid, Astrocytes}, volume = {9}, Number = {2}, pages = {1399-1407}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1760-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1760-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Azizi, Mohammad and hosseini, Rastegar and MohammadZamani, toraj and Samadikhah, ami}, title = {The Effect of l-Arginine Supplementation on Indices of Delayed-Onset Muscle Soreness after One-Session Exhaustive Aerobic Training in Inactive Men}, abstract ={Background & Objective: Nowadays, due to the epidemic of sports supplements, the effects of these supplements on total antioxidant capacity require analysis and evaluation. Therefore, the purpose of the present study is the effect of L-Arginine supplementation on indices of delayed-onset muscle soreness after one-session exhaustive aerobic training in inactive men. Materials & Methods: The population of this quasi-experimental are 20 male that were selected on a voluntary basis. The samples were randomly divided into two groups of L-arginine (n = 10) and placebo (n = 10). The subjects participated in three phases of aerobic activity. Blood samples were collected during the first phase before and after aerobic test, but in the second and third phases after receiving three days supplementation and placebo immediately after aerobic running. For extraction of results the ANOVA with repeated measures and paired t-test were applied and the significant level set as P<0.05. Results: The results indicate that after the pre and post Stage one (before receiving of supplementation and placebo), a significant difference was found in serum creative kinase (CK), lactate dehydrogenase (LDH) and neutrophil counts in both groups. The results between groups indicated that there was a significant difference between two groups in the return of the primary state in blood lactate and heart rate. Also the results shows that CK, LDH and neutrophil counts in L-Arginine supplementation group was significantly lower than Placebo group after the resistance exercise session. Conclusion: The findings of this study indicate that l-Arginine supplementation can cause a significant decrease in indices of delayed-onset muscle soreness (DOMS).  }, Keywords = { Aerobic Training, Muscle Soreness, L- Arginine supplementation }, volume = {9}, Number = {2}, pages = {1408-1417}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1705-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1705-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Izadpanah, Payman and abdollahi, abbas}, title = {The Relation between Prevalence of Positive Anti-chlamydia Pneumonia Antibody Titers and Atherosclerotic Diseases}, abstract ={Background & Objectives: One of the most important of heart problems is related to the occurrence of atherosclerotic plaques. One of the underlying factors in this regard is bacterial infections. Many studies indicate the association between Chlamydia pneumoniae and the creation of plaques; it is one of the most important organisms in the development of coronary heart disease. The aim of this study was to investigate the association between the antibodies against Chlamydia pneumoniae and those relation with coronary atherosclerosis. Materials & Methods: In this descriptive case-control study, serum samples were collected from two groups of patients (108 patients) and healthy subjects (108 persons). Anti-Chlamydia pneumonia antibody titers were evaluated; patients under study were diagnosed with acute myocardial infarction. Both groups were matched in terms of the age and sex to reduce possible interactions. Results: In the evaluation of IgG antibody titers by ELISA and comparison between the two groups, the positive titers were 88.2% in the case group and 60.5% in the healthy subjects; this percentage was 74.6% in all subjects. The results of the two groups were statistically significant (P ≤ 0.005). Conclusion: Regarding the significance of the results in comparison between the patient and the control group, it can be argued that the presence of Chlamydia pneumonia can have a significant relationship with the development of atherosclerotic plaques. Despite the controversial findings in this regard, precise molecular studies can reveal possible mechanisms for the association of bacterial infections and the development of atherosclerosis.    }, Keywords = { Chlamydia pneumonia, Atherosclerosis, Antibody titers, ELISA}, volume = {9}, Number = {2}, pages = {1418-1424}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1819-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1819-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Raoufi, atefeh and sirous, farsi and Hoseini, Ali}, title = {The Effect of Eight Weeks of Curcumin Supplementation on the Expression of Some Regulatory Genes of Atrophic Processes in the Heart Tissue of Fatty Adult Fatty Rats}, abstract ={Background & Objective: Obesity causes heart muscle atrophy. The purpose of this study was to investigate the effect of curcumin supplement on expression of PGC1α, FoxO3a, MuRF1, MAFbx as regulators of cardiac muscle atrophy of rats. Material and Methods: 12 male Sprague Dawley rats were divided into two groups: obese (n = 6) and curcumin supplement (n = 6) after eight weeks of using high fat diet. The rats received curcumin for eight weeks and 150 mg / kg daily. The expression of genes was performed using Real Time-PCR technique. The expression of genes was calculated using the 2-∆∆CTmethod. One-way ANOVA was used to determine the significance of the variables among the study groups. Results: The administration of curcumin resulted in a significant reduction in the expression of genes of FoxO3a (P = 0.008), MAFbx (P = 0.02), MuRF1 (P = 0.001), and a significant increase in PGC1α gene expression (P = 0.004). Conclusion: Probably, administration of curcumin can be used to suppress heart muscle atrophy due to obesity by controlling the muscle atrophy pathway.    }, Keywords = { Obesity, Curcumin, Heart Diseases, Polyphenols}, volume = {9}, Number = {2}, pages = {1425-1432}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1896-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1896-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Torabi, Msoud and Moradi, Mandana and Izadpanah, Fatemeh}, title = {Knowledge and Practice Evaluation of Antibiotic Prescription by Dentists for Dental Infection in Tehran (2014)}, abstract ={ Background & Objective: During recent decades antimicrobial resistance has become a worldwide problem to public health. The prescribing pattern of antibiotics by health care professionals is one of the main factors which affecting antimicrobial resistance. Evidences show the inappropriate use of antibiotics by dentists and could result to the antimicrobial resistance Materials & Methods: The aim of this study was to determine GDPs’ knowledge and practice of antibiotic prescribing by Dentists in Tehran and find the main cuases of irrational prescription and then try to improve policies and education. A questionnaire was sent to 450 participants and receive 270 acceptable cases. The Data were analyzed using T test, ANOVA, by SPSS version 10. Results: Based on prescription of antibiotics for clinical sign data, the mean knowledge score was 4.2, the majority of dentists would prescribe antibiotics for acute pulpitis(78.9%), Acute periapical infection (75.2%), cellulitis (70.1%) and acute ulcerative gingivitis (67.4%) and 21%,19.7%,11.8% of respondents always prescribed antibiotics after prolonged root canal therapy, retreatment and after all root canal therapy. 3.8% of respondents answered all the questions of prophylactic antibiotic coverage correctly. The drug of choice for these cases was 2g Amoxicillin (75.2%). Conclusion: we found the knowledge of General dental practitioner is inadequate about antibiotic therapy and is recommended a reform of their curriculum for further effective education and holding update continuing education programs.    }, Keywords = {Keywords: antibiotic therapy, dental infection, prescription, general dental practitioner}, volume = {9}, Number = {2}, pages = {1433-1442}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1833-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1833-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Sadeghpour, Hossein and Sabet, Elaheh and Dehshahri, Ali}, title = {Preparation of Hydrocortisone Conjugated Polyethylenimine Nanocarriers and its Characterizations for Potential Application in Targeted Gene Delivery}, abstract ={Background & objective: nowadays, gene therapy has been considered as a novel therapeutic approach. Particularly, following the introduction of immunotherapy and gene editing using CRISPR/Cas9 platform great attention has been directed to gene therapy. However, finding an efficient carrier with low toxicity is still one of the major challenges for researchers. Among non-viral gene carriers, polyethyleneimine (PEI) could be considered as the most studied polycationic compound for gene delivery. However, toxicity, lack of targeting and non-specific interactions with serum components limit its wide application. Therefore, PEI structure must be modified through chemical conjugations. In this study, conjugation of hydrocortisone on the polymer structure was carried out in order to direct the carriers into the cell nucleus. Materials & Methods: hydrocortisone was conjugated onto polyethylenimine followed by the grafting of the targeted PEI domain onto another unmodified polymer via succinic acid linker. The conjugate w::as char::acterized in terms of buffering capacity, zeta potential, particle size, protection of DNA against enzymes and condensation ability. Results: The results demonstrated that the conjugates could condense plasmid DNA successfully and form nanoparticles with the size of around 300 nm. Meanwhile, the conjugates showed higher buffering capacity compared with the unmodified polymer. Also, the protection ability of the conjugates was significant rather than the parent polymer. Conclusion: According to the results, design and synthesis of the PEI derivative with two separated domains responsible for targeting and plasmid condensation could be considered as an efficient strategy to create nano gene delivery systems.    }, Keywords = { polyethylenimine, hydrocortisone, targeted gene delivery, polymeric nanoparticle }, volume = {9}, Number = {2}, pages = {1443-1456}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1894-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1894-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Miladpour, Behnoosh and Shafaghat, Marzieh and Owji, Mojdeh and Zal, Fatemeh and Khajeh, Fatemeh}, title = {Evaluation of Potential Effect of Retinoic Acid on the Differentiation of Rat Bone Marrow Mesenchymal Stem Cells into the Beta Cells and Insulin}, abstract ={Background & Objective: Mesanchymal stem cells (MSCs) derived from a variety of human adult tissues and potentiate to self-replicate and differentiate into different cell types. Retinoic acid (RA) is important in embryonic development of pancreas. The effect of RA on transdifferentiation of rat bone marrow mesanchymal stem cells (BMMSCs) into the beta cells was studied. Materials & methods: Rat BMMSCs were prepared by flashing method and confirmed by evaluating of BMMSCs surface markers. BMMSCs were treated in four groups by: 1) rat pancreatic extract (RPE) (250µg/ml) 2) RPE (250µg/ml) + RA (10µM), 3) no treated group as control and 4) ethanol 10% as vehicle and RA(10µM) as control. Insulin secretion was evaluated by ELISA assay. Insulin expression (RT-PCR) were determined. Results: RT-PCR results showed that insulin expression in RPE and RPE + RA groups. Insulin releasing in group RPE + RA was significantly more than group 1, (p-value < 0.05), Wilcoxon test.. Conclusion: Our study showed that RA can promote differentiation of the BMMSCs into the insulin producing cells invitro.    }, Keywords = { retinoic acid, differentiation, beta cells, BMMSCs}, volume = {9}, Number = {2}, pages = {1457-1463}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1824-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1824-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Zeynali, Zahra and AbtahiFroushani, Seyyed Meysam}, title = {The Effects of Nicotine and Caffeine Treatment on the Vitality and Regenerative Potentials of Mesenchymal Stem Cells}, abstract ={Background & Objective: Previous studies indicated that Mesenchymal stem cells (MSCs) express some of the nicotinic receptor subunits and adenosine receptors. Therefore, MSCs function may be controlled by two very consuming environmental substance like nicotine (nicotinic receptor-agonist) and caffeine (adenosine antagonist). The purpose of the present study is to determine the role of nicotine or caffeine on the some of the function of MSCs. Materials & methods: The mesenchymal stem cells were isolated from the bone marrow of NMRI. The cells were incubated with caffeine (0.1mM) and or with nicotine (0.1µM) for 48 hours. In a set of experiments, the cells were trypsinized and the vitality, the biological activity of cell membranes and the potentials mitochondrial redaction of them were assumed. In another experiments, the cutler plates were scratched with tip of the sampler, and the regeneration velocity of scar was evaluated by an optical camera. Results: Treatment of MSCs with nicotine caused a significant increase in the absolute number of MSCS, concurrent with an increase in mitochondrial function. While caffeine decreased the vitality of cells, it also promoted a significant reduction in mitochondrial activity of remnant live cells. Nicotine induced a significant reduction in membrane activity of cells, while caffeine treatment exhibited a marked raise in neutral red uptake by MSCs compared to neutral red uptake by control cells. Regenerative assay showed that nicotine markedly promoted the regenerative potential of MSCs, while caffeine markedly reversed the regenerative potential of MSCs. Conclusion: It seems that nicotine at used dose promoted the regenerative potential of MSCs, while caffeine had a revers effects.    }, Keywords = {Mesenchymal Stem cell, Caffeine, nicotine}, volume = {9}, Number = {2}, pages = {1464-1473}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1758-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1758-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {yousefisaggezi, saman and azamianJazi, akbar and Hemmati, Roohullah and jivad, nahi}, title = {Effects of combined exercise (aerobic + resistance) on the expression of miR-301a and some indicators of motor function in women with multiple sclerosis (MS)}, abstract ={Background & Objective: Several evidence has indicated the effective role of exercise on Multiple sclerosis (MS) patients. An aberrant expression of some Micro-RNAs such as increasing miR-301a in MS, with an impact on inflammatory pathways leading to the creation of inflammation in these patients. The purpose of this study was to investigate the effect of a combined eight-week (aerobic and resistance) exercise on expression of miR -301a and some indicators of motor function in women with multiple sclerosis. Materials & Methods: A total of 22 women with MS, who were randomly divided into experimental and control groups, participated in this study. The experimental group performed 8 weeks (4 days per week) of combined training. Expression of miR -301a was measured before and after the training period using Real-Time PCR method. Pre- and post-intervention scores of tests (TUG), (6MWT), (10–MWT) and status scale (EDSS) were independent t-test recorded and analyzed. Results: The results of this study showed, the expression of miR -301a was significantly different between the two groups (P = 0.001). Tests TUG (P = 0.001), 6MWT (P = 0.002) and degree of disability EDSS (P = 0.015), were significantly different between the two groups. There was no significant difference between the 10MWT test between the two group (P = 0.678). Conclusion: The combined exercise for women with MS have been beneficial and improve motor function and reduce the expression of miR-301a. So, do it, plays an important role in reducing inflammation and prevent the progression of the disease Ms.    }, Keywords = {MS, combined exercise, miR -301a, Motor function, EDSS}, volume = {9}, Number = {2}, pages = {1474-1486}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1926-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1926-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {heydari, esfandiar and hosseini, ali and azarbayjani, mohammad ali}, title = {The Effect of Eight Weeks Resistance Training with Two Doses of Crocin Consumption on Catalase and Glutathione Peroxidase in Kidney Tissue of Nandrolone Poisoned Rats}, abstract ={  Background & Objective: The use of anabolic steroids causes damage to various tissues, including the kidneys. Aim of present study was to investigate the effect of resistance training with consumption of crocin on catalase and glutathione peroxidase in kidney tissues of nandrolone poisoned rats. Material & Method: Thirty rats selected and assigned to 6 groups 1) nandrolone, 2) nandrolone + training, 3) nandrolone + 12.5 mg/kg crocin, 4) nandrolone + 25 mg/kg crocin, 5) nandrolone + training + 12.5 mg/kg crocin, and 6) nandrolone + training + 25 mg/kg crocin. Groups 2, 5 and 6 performed resistance trainings for eight weeks and three sessions per week and groups of 3 to 6 received daily doses of crocin. To analyze the findings of the study, one-way ANOVA and Bonferron's post hoc tests were used (p≤0.05). Results: 12.5 mg/kg crocin, 25 mg/kg crocin, training + 12.5 mg/kg crocin and training + 25 mg/kg crocin have significant effect on reduction of catalase (p=0.001), training, training + 25 mg/kg crocin have significant effect on increase of glutathione peroxidase (p=0.001) also training + 25 mg/kg crocin rather than training + 12.5 mg/kg crocin has more effect on increase of glutathione peroxidase (p=0.001). Conclusion: Although resistances trainings with consumption of 25 mg/kg crocin have an interactive effect on increase of glutathione peroxidase; however, resistance training, crocin consumption and the simultaneous use of crocin and resistance training reduce catalase in the kidney tissue of nondronean poisoned rats.}, Keywords = { Nandrolone, Resistance Training, Crocin, Catalase, Glutathione Peroxidase}, volume = {9}, Number = {2}, pages = {1487-1497}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1891-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1891-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Banafsheh, Hamidreza and Gilasi, Hamidreza and Ghaderi, Amir}, title = {Survey on Patients\' Satisfaction with the Services Provided at Pharmacies in the City of Kashan}, abstract ={Background & Objective: Patients’ satisfaction is a key factor in the evaluation of health system’s quality in developed countries. The purpose of the presnt study was to investigate Patients’ satisfaction with services provided at pharmacies in the city of Kashan and related elements. Materials & Methods: In a cross- sectional study, 440 patients who referred to pharmacies in the city of Kashan were surveyed randomly. The questionnaire was composed of 44 items and focused on four dimensions: patients’ expectations from the pharmasict, pharmacist’s communication skills, consultations offered by the pharmacist, and the location and physical environment of the pharmacy. In order to analyze the data of this study, SPSS software version 20, and parametric and ANOVA tests were used. Results: Patients’ highest expectations were courteous behavior from the pharmacist and the personnel (92/7%), low wating time (92%), explanation for medication indications (85%), and explanation on how to use the medications (82/5%). Patients’ satisfaction elements with the highest score were cordial and pleasant attitude of the pharmacist (78.9%), labeling the medications by the pharmacist (78%) and the illumination inside the drugstores facility (93%). Conclusion: The city of Kashan has variable levels of satisfaction with the quality of pharmacy services. To increase levels of satisfaction, it is necessary to provide appropriate training in pharmacies and quality of services promted in all domains.    }, Keywords = { Patient satisfaction, Pharmacy services, Patient experience, Pharmacist–patient communication}, volume = {9}, Number = {2}, pages = {1498-1506}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1813-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1813-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Asadmanesh, enayatollah and KoushkieJahromi, Maryam and Daryanoosh, Farhad and Neamati, Javad and Mojtahedi, Zahr}, title = {The Effect of Six Week Resistance Training on Muscle Regeneration in Tumor- Bearing BALB-C Mice}, abstract ={Background & Objective: Many cancer patients suffer from cachexia or cancer-induced muscle atrophy. Cachexia can have various causes one of which is the reduction of muscle regeneration. Resistance training has been suggested as one of the proper stimulator of increasing muscular regeneration. The present study aimed at evaluating the effect of resistance training on two factors of regeneration including PAX7 and eMHC, tumor-free weight and tumor weight of mice.  Materials & Methods: This study was a kind of experimental intervention. Subjects of the study included 10 BALB-C mice (age: 6 weeks) which CT-26  tumor was transplanted to them. Mice were divided into two groups of resistance training (n=5) and control (n=5) randomly. Training group performed six-week progressive resistance training and control groups were kept in cages without any exercise intervention. At the end of the experiment, gastrocnemius muscle was taken for evaluating related factors. Data were analyzed using the independent t-test. Results: There was no significant difference in PAX7 between two groups of training and control, but eMHC reduced significantly in training compared to the control group (P=0.038). Tumor-free bodyweight of training group increased significantly compared to the control group (P=0.0004) and there was no significant difference in tumor weight between two groups of training and control. Conclusion: Although resistance training does not increase tumor growth but probably reduce some muscle regeneration factors in cancer-bearing mice. So, for improving muscular regeneration in cachexia bearing patients, probably resistance training is not a good choice. However, more future researches are required.}, Keywords = {Cachexia, muscular regeneration, resistance training, PAX7, eMHC}, volume = {9}, Number = {3}, pages = {1507-1514}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1732-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1732-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Dargahi, Hossein and Rahbar, Ahmad and zolfagharinia, Alireza and gharlipour, zabihollah and Ramezani, tahereh and kazzazlou, Zohreh}, title = {Relationship Between Rate of Access to Resources and Guidelines of Health Information and Quality of Life in Women Referring to Hospitals in Qom in 2016}, abstract ={Background & Objectives: In Iran, few studies have been conducted on health information sources. The aim of this study was to determine the relationship between rate of access to resources and guidelines of health information and quality of life in women referring to hospitals in Qom city in 2016. Materials & Methods: This cross-sectional descriptive-analytical study was performed on 254 women referring to hospitals in the city of Qom in 2016. The subjects were selected via multi-stage sampling method. Data were collected by a researcher-made questionnaire on sources and guidelines of health information and the valid Quality of Life Questionnaire. The collected data were entered into SPSS V.20 software and were analyzed using Chi-square, ANOVA, and Pearson correlation coefficient tests. Results: There was a significant negative correlation between the quality of life score and the use of the resources and guidelines of health information (r=-0.14, p=0.02), as with increasing the use of the resources and guidelines of health information, the score of quality of life of the subjects reduced. In addition, there was a significant relationship between educational level and type of the resources and guidelines of health information (p<0.02) so that, the people with university education were more likely to use more self-resources and mass media to obtain health information. Conclusion: Given the significant relationship between quality of life and the use of the resources and guidelines of health information, people should be properly informed about and familiarized with the reliable sources and guidelines of health information that are suitable for their conditions and levels of literacy.  }, Keywords = {کیفیت زندگی, میزان دسترسی, اطلاعات سلامت, راهنماها}, volume = {9}, Number = {3}, pages = {1515-1524}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-2059-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-2059-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Rostami, Dariush and abdollahi, elahe and taghizadeh, hassan and gheibihayat, sayed mohammad and taghizadeh, eskandar}, title = {Angiogenesis and Metastasis with a Therapeutic Approach: A Review}, abstract ={Angiogenesis is a process in which new blood vessels produced from old vessels and this process plays an important role in the natural processes in body. Also, angiogenesis in tumor cells leads to further growth of these cells and leads to their metastasis. This process involves migration, proliferation and differentiation of endothelial cells. One of the new ideas that attracted the attention of scientists is to inhibit the angiogenesis process in cancer patients. Scientists are hoping that this method leading to a tumor cells therapy. The purpose of this study was to review the role of angiogenesis in metastasis of tumor cells with a therapeutic approach.}, Keywords = {Angiogenesis, Tumor, Metastasis}, volume = {9}, Number = {3}, pages = {1525-1533}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1594-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1594-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Sanaei, Masumeh and Kavoosi, Fraidoo}, title = {The role of histone deacetylases in human gastrointestinal and associated glands cancer}, abstract ={Background & Objective: In the eukaryotic cells, the DNA wraps around the histone proteins (H2A, H2B, H3, and H4) and constitutes the nucleosome. Chromatin organization plays a major role in the control of gene expression. Epigenetic modifications can induce a reversible change in the chromatin structure as being open and accessible DNA to the transcriptional factors resulting in gene expression. Histone modification, as an epigenetic factor, is necessary for gene expression. These modifications are concluded in a balance between histone acetyltransferase and histone deacetylase activity. Histone deacetylation compacts chromatin structure resulting in gene silencing. Tumor suppressor genes play an important role in the cancer prevention. Deacetylation of these genes resulting in genes silencing and carcinogenesis. In this review, we will evaluate the effects of histone deacetylases on the gastroinetestinal tract and associated glands cancer. Methods: For this review article, we search different online sources by various researcher motors including Scopus, PubMed, and ISI resulted in finding articles correlated with the effect of histone deacetylase on the gastroinetestinal tract and associated glands cancer. Conclusions: In the current study, we concluded that histone deacetylases can induce cancer by histone deacetylation of tumor suppressor genes in the gastrointestinal tract and associated glands.  }, Keywords = {Histone deacetylases, Tumor suppressor genes, cancer}, volume = {9}, Number = {3}, pages = {1534-1541}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1910-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1910-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {karimian, maryam and Ghanbarzadeh, Mohsen and Habibi, Abdolhamid and nikbakht, masoo}, title = {The Effect of Two Methods of Aerobic Exercise on IFN-γ in the Animal Model of Experimental Autoimmune Encephalomyelitis}, abstract ={Background & Objective: Multiple Sclerosis (MS) is a chronic demyelination of the central nervous system which has caused a lot of complications for affected patients and leads disability. Today, along with other sciences, the field of research of sports science is trying to find ways to improve disorders caused by diseases and sport injuries and to improve the health of people in the community. Regarding the importance of complementary therapies, this study aimed to investigate the effect of two types of aerobic training on inflammatory cytokines of IFN-γ in animal samples with MS. Materials & Methodes: The sample included 96 C57bl6 mice. After preparation and induction in the form of EAE model, swimming training was conducted in 8 groups of 6, including healthy groups (control, swimming) and MS (control, swimming, interferon, swimming + interferon, control, injection and control). Moreover, in the case of wheel running training, they were divided into eight groups of 10 including healthy groups (control, wheel running) and MS (control, wheel running, interferon, wheel running + interferon, injection control, wheel running control and injection). The animal models performed the exercise protocol after one week of environmental compatibility. After blood sampling, IFN-γ concentration was measured with specific kits in the laboratory by separating serum. At the end of the experiments, the severity of EAE in rats were compared among the study groups. The present study was done experimentally and all data and raw data obtained from the samples were analyzed by SPSS (software version 24) using one-way ANOVA. The minimum level of significance was considered as p≤0.05. Results: The study showed that the mean concentration of IFN-γ in the EAE model, which was performed by the swimming, had a significant decrease both in the non-injected (p=0.047) and interferon-treated group (p=0.013) compared to the non-active models. Also, the mean concentration of IFN-γ in wheel running training only in EAE group and wheel running was significant.(p=0.025) p≤0.05. Conclusion: According to the protocol of the present study, it can be concluded about the response of inflammatory markers to aerobic training courses that the physical activity of swimming and wheel running has been able to reduce the level of inflammatory markers with MS.    }, Keywords = {Experimental autoimmune encephalomyelitis, IFN-γ, swimming, wheel running}, volume = {9}, Number = {3}, pages = {1542-1551}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1829-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1829-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Bagheri, Aboutaleb and Shakeri, Nader and NikBakht, Hojatollah}, title = {The Effect of 8-week Aerobic Physical Activity on Hs-CRP and Blood Pressure of the Shift Workers of Sarir Plast Industrial Group in Dezful}, abstract ={ Background &Objective: Shift work can be a factor in inflammation, hypertension and cardiovascular diseases. The purpose of the present study was to evaluate the effect of 8-week aerobic physical activity on Hs-CRP and blood pressure of the shift workers of Sarir Plast Industrial Group in Dezful. Materials & methods: The present research is a semi-experimental research that was carried out at the shift workers of the five industrial group of Surirplast. 30 workers, who had an average of 5 years of shift work history, were selected using judgmental sampling technique and were then randomly assigned to the two groups of aerobic physical activity (n=15) and control group (n=15). The experimental group performed 8 weeks, 3 sessions per week with 50-70% THR intensity, according to the schedule. Before and after the plan, the activity, height, BW, BMI, WHR, BFP, VF, Hs-CRP, SBP, DBP and HR were measured. For data analysis, Kolmogorov-Smirnov test, Leven test, Mbox-test and ANCOVA were used (p<0.05). Results: The BW, BMI, WHR, BFP, and VF decreased significantly in the post test for the training group, while the value of Vo2max increased (p<0.05). Also, the results of the study indicated that APA had a significant effect on BW, BMI, WHR, BFP, VF, Hs-CRP, SBP, DBP, and HR. Conclusion: 8-week Aerobic physical activity was effective in reducing the risk factors of CVD in shift workers; perhaps aerobic physical activity could be used as a non-pharmacological approach to prevent CVD in shift workers.}, Keywords = {Shift Work, Hs-CRP, Blood Pressure, APA}, volume = {9}, Number = {3}, pages = {1552-1563}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1908-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1908-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Mirzaei, Mansooreh and Shahrooz, Rasoul and ShalizarJalali, Ali and Sarrafzadeh-Rezaei, Farshid and Mosaffa, Narim}, title = {The Effects of Fibroblast and Macrophage Separated and Co-Culture Conditioned Medium on Sperm Parameters Disorders Induced by Testicular Torsion-Detorsion in Adult Rats}, abstract ={ Background & Objective: Ischemia and Reperfusion (I/R) lead to the production of excessive reactive oxygen species (ROS). In the present study the protective effects of the cell culture conditioned medium (CM) on sperm quality disorder induced by torsion-detorsion (T-D) in adult rat’s testis was evaluated. Materials & methods: In this study 42 adult rats were divided into seven groups. 1- Normal control group treated without surgery. 2- Laparotomy group, surgery operated without T-D. 3- T-D group, surgery operated along with T-D. 4- T-D+DMEM group, after T-D Dulbecco's Modified Eagle's Medium (DMEM) has been injected. 5-7 groups included T-D+F, T-D+M, and T-D+FM, along with T-D injected conditioned medium of fibroblast, macrophage, and fibroblast-macrophage co-culture respectively. All injections were made at a rate of 10μL once after T-D in the rete testis. After 35 days, sperm quality parameters were evaluated and obtained data were compared between groups by one-way ANOVA statistic method and Tukey test post hoc in P<0.05 level. Results: The experimental groups that received CM of the fibroblast and macrophage compared to groups T-D and T-D+DMEM there were showed ameliorative effects in comparison to T-D and T-D+DMEM groups. There was not observed any ameliorative effects in the T-D+FM group. Conclusion: The results of this study showed the high ability of supernatant of the separate fibroblast and macrophage culture and in improving sperm parameters after testicular T-D.  }, Keywords = {Testicular torsion-detorsion, Fibroblast, Macrophage, Sperm quality, Rat}, volume = {9}, Number = {3}, pages = {1564-1576}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1880-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1880-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {RoshaniAsl, Parisa and Rashidi, Niloufar and Shokoohizadeh, Leili and Tajbakhsh, Elahe}, title = {Survey of Antibiotic Resistance and Relationship Between Eesterase (estA) Gene with Biofilm Formation in Pseudomonas Aeruginosa Strains Isolated from Burn Patients}, abstract ={Background & Objective: Pseudomonas aeruginosa is known as a major cause of hospital-acquired infections due to its high antibiotic resistance. Biofilm formation is an important virulence factor in P. aeruginosa infections. This pathogen produces extracellular hydrolases such as esterase estA during biofilm formation which can influence the formation and construction of biofilm. The purpose of this study was to detect the antibiotic resistance and distribution of estA gene among biofilm-producing P. aeruginosa strains isolated from burn patients. Materials & Methods: A total of 37 strains of P. aeruginosa were isolated from burn patients in Taleghani hospital in Ahvaz city and identified using standard bacteriological procedures. Antibiotic susceptibility test was performed by disk diffusion method according to the CLSI 2015. Biofilm formation was measured by micro titer plate. Existence of estA gene was detected by PCR. Results: The estA gene existed in 97.3% of isolates and 78.3% of P. aeruginosa isolates produced biofilm. Based on the results of the antibiogram test, highest rate of resistance was observed to piperacillin/ tazobactam (92%) and least resistance was to colistin (8%). Conclusion: According to the results, there was no significant correlations between presence of estA gene and biofilm formation. High level of resistance to antibiotics in P. aeruginosa is considerable.    }, Keywords = {Pseudomonas aeruginosa, estA, Biofilm, Antibiotic Resistance}, volume = {9}, Number = {3}, pages = {1577-1584}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1790-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1790-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {MirBalochi, Rahmatolah and Salesi, Mohsen and ChardahCheric, Majid and KoushkiJahromi, Maryam and Sadeghipour, Hamid Rez}, title = {The Effect of One Session Exhaustive Exercise on Hepcidin, Iron, Ferritin and Hemoglobin of Female Athletes}, abstract ={Background & Objective: Females athletes are at increased risk of depleting their iron stores and iron deficiency. It has been suggested recently that hepcidin may be an important regulator of whole-body iron homeostasis. Then the purpose of the present study was to investigate the effects of exhaustive exercises on hepcidin hormone, iron, ferritin and hemoglobin of female athletes. Materials & Methods: 30 female athletes were selected for the study and were randomly assigned to experimental and control groups. The participants' fasting blood samples was taken 2 h before exercise (including 3*6 repetition), just, 3 and 24 hours after exhaustive exercise. Data analysis was carried out using repeated measure and ANOVA. Results: The results showed that hepcidin increased significantly after the exercise in the experimental group (P= 0.001) and the highest increase was 3 hours after exercise (P= 0.004). Also Iron (P= 0.001), ferritin (P= 0.001) and hemoglobin (P= 0.001) increased significantly just after exercise. However, the hemoglobin value during 3 hours post exercise decreased Compared to pre exercise (P= 0.01). Conclusion: It is concluded that a session of acute exercise can increase the hepcidin and so that help to absorb and maintain of iron.}, Keywords = {Hepcidin, Exhaustive Exercise, Female Athlete}, volume = {9}, Number = {3}, pages = {1585-1595}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1933-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1933-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {zargar, mohsen and javadi, ali and morovvati, abbas}, title = {Detection of Clostridium difficile associated diarrhea in disease based on Polymerase chain reaction and bacterial culture And toxin A, B frequency}, abstract ={Detection of Clostridium Difficile Associated Diarrhea in Disease Based on Polymerase Chain Reaction and Bacterial Culture and Toxin A, B Frequency Detection of Clostridium Difficile Associated Diarrhea in Disease Background & Objective: Clostridium difficile is an obligate anaerobic, gram positive bacillus. The purpose of this study was to comparison between PCR technique and bacterial culture for assessing the prevalence of Clostridium difficile infection in the samples of watery diarrhea. Materials & methods: This cross-sectional qualitative study was performed in 68 samples of watery diarrhea in Qom province hospitals. All samples were cultured in the specialized CCFA Agar medium. Specific primers were applied for the PCR Assay based on cdd3 gene. Based on this primer PCR product for this gene must be 622bp.toxin A & B diagnosis based on specific primers and product must be 473 and 272 bp Results: In this study, the optimized PCR technique was determined to be bacterium in 16 samples. But bacteria diagnosis in 11 samples by culture method. 6 samples have toxin A and B, 4samples has toxin A and 3 samples has toxin B. Conclusion: Cytotoxicity method is gold standard for clostridium difficile. Diagnosis of these bacteria is very important and PCR was found as an applicable, sensitive, and quick technique for detection of Clostridium difficile in compromise culture medium method.  }, Keywords = {diarrhea, PCR, ccd3 gene, Clostridium difficile, toxin A, B}, volume = {9}, Number = {3}, pages = {1596-1604}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1889-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1889-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Rahdar, Abbas and Ghasemi, Behzad and Hashemi, Seyed Hasan and Moghaddammanesh, Mohammad rez}, title = {Antibacterial Effect of New Compounds Against Lactococcus garvieae}, abstract ={Background & Objective: Lactococcus garvieae is one of the most important zoonotic pathogens that threats the aquaculture industry. The recognition and use of new antibacterial compounds is one the best ways to control of this pathogen.In this study, inhibitory effects of Zn nanoparticles, thioethers derivatives, poly-L-lysine, nisin, glycine and hydroalcoholic extracts of Rosemary and Prosopisfarctaleaves were assessed against L. garvieae. Materials & methods: Antibacterial effect of all compounds were evaluated via the disk diffusion and broth microdilution methods. The results were reported as inhibition zone diameter (IZD), the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) values. Results: Any significant inhibitory effect was observedon L. garvieae with poly-L-lysine, nisin, glycine and hydroalcoholic extracts of Rosemary. Antibacterial effectof twothioethers derivatives, Zn nanoparticles and hydroalcoholic extracts of Prosopis farcta leaves was observed with IZD = 9.48-18.35 mm, MIC = 32-4096 µg/ml and MBC = 64-2048 µg/ml.The best inhibitory effect was belonged tothioethers derivative 4e. Conclusion: Antibacterial effect of thioethers derivatives, Zn nanoparticles and hydroalcoholic extracts of Prosopis farcta leaves were proven on L. garvieae invitro. New ligands and complexes can be designed according tested thioethers especially derivative 4e in future researches to improve antimicrobial properties.    }, Keywords = {Antibacterial Effect, New Compounds, L.garvieae}, volume = {9}, Number = {3}, pages = {1605-1612}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1946-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1946-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Salehi, Fateme and Moghaddam, Ali-Asghar and Rahimi-Feyli, Peym}, title = {Effect of Vitamin E on Induction of Colonization of Spermatogonial Stem Cells in Vitro}, abstract ={Background & Objective: Spermatogonial stem cells (SSCs) are undifferentiated germ cells that maintain spermatogenesis during lifetime by balancing between self-renewal and differentiation. The aim of this study was to investigate the effect of different concentrations of vitamin E on spermatogonial stem cells colony formation. Material & Methods: SSCs were isolated from testes of prepubertal lamb by two step enzymatic digestions then purified by differential pllating. The cells were cultured for 10 days in 4 groups. Control group: Basic media (DMEM environment which contains 1% antibiotic and 5% FBS( and treatment groups were treated with 20, 40 and 80 µmol/ml vitamin E added to basic media respectively. Changing of culture media was performed every 72h. Colony number and diameter assay was done by inverted microscope. Identification of SSCs was performed by immunocytochemistry assay against PGP9.5. Results: there were no significant difference in colony number and surface area between treatment groups and between treatment and control groups in days 4, 7and 10. Colony surface area was significantly increased at day 10 compared to day 4 (P<0.05). Conclusion: The results of this study showed vitamin E as an antioxidant doesn’t have significant effect on colony induction of SSCs in short term culture in vitro.}, Keywords = {Spermatogonial Stem cell, Vitamin E, In-Vitro}, volume = {9}, Number = {3}, pages = {1613-1620}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1706-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1706-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {asgarirad, hossein and farmoudeh, ali and rezaeiroshan, anahita and rajaei, mohammadjav}, title = {Preparation and Characterization of Paracetamol Solid Dispersions Using Opened Ring PVP and PEG 4000}, abstract ={Background & Objective: Solid dispersions (SDs) have been traditionally used as an effective method for improving the dissolution properties and bioavailability of poorly water-soluble drugs. The aim of this study was to improve the solubility and dissolution rate of paracetamol by SD technique. Materials & methods: The prepared SDs were evaluated by saturation solubility test, In-vitro drug release test, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). Results: The prepared SDs exhibited a statistically significant increase in the solubility of paracetamol compared to that of the free drug (p < 0.05). After 15 min SD tablets had an enhanced cumulative drug release compared to tablets of the free drug (p < 0.05). FTIR study revealed that paracetamol was stable in polymeric dispersions. DSC and SEM microscopy showed that the drugs crystallinity was decreased during the preparation process (amorphous crystal formation). Conclusion: The FTIR spectroscopic test revealed the presence of intermolecular hydrogen bonding between paracetamol and the polymers in the SDs, which could increase the aqueous solubility of the drug. The DSC analysis indicated that the drug was in the amorphous state when dispersed in the polymers. Dissolution studies indicated that the dissolution rates were markedly increased in the SDs compared with those of paracetamol alone, and Better results were obtained with PVP K25.    }, Keywords = {Solid dispersion, PEG 4000, PVP K25, Paracetamol}, volume = {9}, Number = {3}, pages = {1621-1631}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1944-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1944-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Jirofti, Nafiseh and Mohebbi-Kalhori, Davod and Hadjizadeh, Afra and Kazemzadeh, Gholam Hosein and Taheri, Rez}, title = {Experimental Study and Evaluation of Mechanical Properties of Nano-Scale Single and Composite Structures (PCL/PU) Fabricated by Co-Electrospinning and Blend-Electrospinning Methods.}, abstract ={Background & Objective: Extracellular matrix (ECM) as a three-dimensional structure consisting of protein nanofibers and polysaccharides with a diameter of 50-500 nm is a place to store cells. Electrospinning is a method that allows nanofibers to be produced in this range and even beyond. Materials & Methods: Polycaprolactone, polyurethane, and required solvents chloroform, ethanol, Tetrahydrofuran, dimethylformamid and 1,1,1,3,3,3-Hexafluoro-2-propanol were used without any purification. Single and composite scaffolds were fabricated of these polymers using Co-electrospinning and Blend-electrospinning methods. The morphological and mechanical properties of the neat and composite structures were evaluated through SEM, FTIR, and Tensile tese. Result: Results showed that the average fiber diameter and Young’s modulus changes’ range in composite and single structures obtained 228 ± 89 to 470 ± 95 nm and 1.2 ± 0.39 to 8.03 ± 0.54 MPa. Composite structures fabricated using Co-electrospinning method have smaller mean fiber diameter 228 ± 89 nm and composite structures fabricated using blend electrospinning have highest Young’s modulus 8.03 ± 0.54 MPa comparison single structures.  Conclusion: The results showed that the produced composite structure meets the required mechanical properties to be used in tissue engineering and, because of its structural and morphological features, can be also applied as alternative scaffolds in the body. Composite structures produced by blend electrospinning method show a higher degree of strength compared to similar composite structures that produced by co-electrospinning method due to the free movement of the polyurethane nanofiber and the intrinsic contraction in the composite structure.  }, Keywords = {Electrospinning, Composite Structures, Polycaprolactone, Mechanical properties, Polyurethane}, volume = {9}, Number = {3}, pages = {1632-1645}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1939-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1939-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {etemaddar, narges and mahvishirazi, majid and esmaeili, masomeh and gholampoor, usef}, title = {The Relatinship Between Personality Traits and Coping Strategies in the Alexithymia Prediction of Chronic Obstructive Pulmonary Patients Referred to Valiasr Hospital in Fasa in 2016}, abstract ={Background & Objective: The purpose of this study was to investigate the relationship between personality traits and coping strategies in the alexithymia prediction of chronic obstructive pulmonary patients referred to Valiasr Hospital in Fasa, Iran. Materials & Methods: The statistical population of this study included all patients referring to the specialized clinic of Vali Asr Hospital in Fasa, which were selected by random sampling method of 180 people based on Cochran sample size formula. In this study, three Alexey Times Toronto Questionnaires (FTAS-20), Neo-Personality Traits Questionnaire (NEO-IP-R), and Lazarus and Folkman Coping Strategies were used, Data were analyzed using SPSS software and correlation method and stepwise regression analysis were used. Results: The results showed that there was a significant correlation between neuroticism, extroversion and agreeableness personality traits and emotional coping strategies(P =0.000). However, no significant relationship was found between personality trait openness and conscientiousness and coping strategies. Also, the excitement style (P = 0.745; Beta = 0.745), extroversion (P = 0.331, Beta = 0.300), neuroticism (P =.0000 Beta =.288) and agreement P = 0.098 Beta =.098) can predict Alexithymia in patients with Chronic Obstructive Pulmonary Disease (COPD). But age, gender, and education were not good predictors of the Alexei time in Copd patients. Conclusion: The results of this study showed that the personality factors of neuroticism, extraversion and consensus-seeking can predict the changes in Alexis time in both positive and negative directions, and the anti-emotional coping style is also negatively predicted by Alexei Time.}, Keywords = {personality traits, coping strategies, alexithymia, COPD}, volume = {9}, Number = {3}, pages = {1646-1656}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1620-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1620-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Faramarzi, Mohammad and Roosta, Sareh and Behzadi, Amineh}, title = {Comparison of Surgical Techniques as a Prognostic Factor on Success Rate in Chronic Otitis Media}, abstract ={Background & Objective: Review of literature show that there are contradictory opinions, regarding the surgical technique act as a prognostic factor in graft success rate. Therefore, the researchers decided to consider the topic by reviewing the literatures and gathering their ten years experiences information regarding this issue. Materials & Methods: This retrospective study was carried out on ears undergoing chronic otitis media surgeries. Five hundred and five ears underwent chronic otitis media surgeries were evaluated. We analyzed 225 tympanoplasty (44.6%), 93 canal walls up mastoidectomy (18.4%) and 187 canal wall down mastoidectomy (37%). The primary outcome was anatomic results (graft success rate in each surgical technique) and the secondary outcome was hearing outcomes. Results: Total graft success rate was found in 460 ears (91.1%). Also graft success rate in tympanoplasty, canal wall up mastoidectomy and canal wall down mastoidectomy groups were observed in 201 ears (89.3%), 81 ears (87.1%) and 178 ears (95.2%), respectively. But there was not significant statistical difference between different methods (P=0.14). The mean post-operation air-bone gap gains in these three surgical techniques were 7.8, 11.1 and 4.3 dB, consecutively Conclusion: However, our results showed that the overall graft success rate was in an acceptable range and graft success rate in canal wall down mastoidectomy was more than tympanoplasty and canal wall up mastoidectomy, but the surgical technique was not a prognostic factor in graft success rate.    }, Keywords = {Otitis Media, Tympanoplasty, Mastoidectomy}, volume = {9}, Number = {3}, pages = {1657-1664}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1825-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1825-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {SadatShirazi, Seiedeh Razieh and Hesampour, Ardeshir}, title = {Quantitative Comparison of CK19 Gene Expression Between Normal Iranian Women and Ovarian Cancer Blood Sample by Real Time PCR}, abstract ={Background & Objectives: Ovarian cancer is the fifth leading cause of cancer deaths in Iran and the United States women population. Late detection of illness could lead to death of the patient and has a lot of cost expenses to economy, so early diagnosis of pain and costs imposed for patients will be reduced.CK19 has the potential for early diagnosis of ovarian cancer. In this study, the potential for gene expression of CK19 as a molecular biomarker for the diagnosis of ovarian cancer in the circulatory system was investigated using Real Time PCR technique, so that it could be further investigated evaluated about the mechanism and effect of this gene, the title is a good target for treating ovarian cancer. Material & Methods: In this case-control study, 50 blood samples from women with ovarian cancer and 50 normal control blood samples from patients referred to Imam Khomeini Hospital were reviewed. After collecting samples, extraction Total RNA from frozen ovarian epithelial blood samples, cDNA was performed from the cDNA. Finally, the expression of the CK19 gene in 50 patient blood samples and 50 normal blood samples was investigated using qRT-PCR technique. Results: We determined that CK19 was significantly up-regulated in ovarian cancer, compared to normal blood sample. Further investigations revealed that this overexpression is elevated by increasing the malignancy stage. Conclusion: This test showed a significant increase CK19 in patients, expression of CK19 was quantitatively measured and expression of CK19 was reported in the serum of positive patients. Also according to grade the patient was different in the serum of patients with CK19 and the higher the patient progressed the higher the expression.  }, Keywords = {Ovarian cancer, CK19 Marker, Real Time PCR}, volume = {9}, Number = {3}, pages = {1665-1674}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1612-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1612-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Nasiri, Davoud and Razavilar, dadoud and Motalebi, Abbasali}, title = {Investigation of campylobacter jejuni caused by Guillain-Barré syndrome in poultry meat and edible offal’s}, abstract ={ Background & Objectives: Campylobacter infections as a common disease are a major contributor to human infectious gastroenteritis and in addition to watery and bloody diarrhea, it causes secondary illness. Although there are many studies of the prevalence of poultry meat to Campylobacter spp but little information was available on the contamination of poultry meat products with the aim of studying the contamination of these products  Material & Methods:   A total of 552 samples of meat (138 samples), liver (138 samples), gizzard (138 samples) and chicken heart (138 samples) were randomly collected from poultry industry slaughterhouses in West Azarbaijan Province. The aim of this study was to determine the presence of Campylobacter spp. in phenotypic and genotypic experiments. Results: The highest prevalence of Campylobacter spp was in the liver (49.2%), followed by gizzard (42.8%), heart (33.3%) and chicken (25.4%). The most common species of Campylobacter spp was Campylobacter jejuni (78.4%) and the remaining Campylobacter spp (21.6%) Conclusions: 208 isolates of Campylobacter spp.., Differentiated by Campylobacter jejuni and Campylobacter coli, were confirmed by polymerase chain reaction (m.PCR). The results of this study show the importance of poultry food sources as a potential source of Campylobacter infection.    }, Keywords = {Campylobacter, Poultry Meat, Offal’s Edible}, volume = {9}, Number = {3}, pages = {1675-1681}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1703-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1703-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {saffar, Mahsa and Alipanah, Hiva and Ataollahi, Mohammad Rez}, title = {The Role of Biomarkers in Diagnosis, Prognosis, Treatment, Determining Disease Activity in Rheumatoid Arthritis}, abstract ={Rheumatoid arthritis (RA) is a chronic inflammatory and complex autoimmune disease. It affects mainly small joints (of the hands and feet) and has many systemic manifestations. The study of biomarkers in rheumatology is important to understand the mechanism involved in some rheumatic diseases. Discovering new biomarkers with key roles in various stages of the disease remains as an important issue in RA patients. Biomarkers are important for diagnosis and prognosis, target therapy, and guiding the clinical and response treatment of all phases of RA. Biomarkers improve diagnosis by closing the serological gap, providing prognostic information that allows disease activity and progression to be monitored. Biomarkers can be correlated with a risk of developing RA and can predict bone erosions and disease progression. Therefore, there is a need for a sensitive biomarker for early diagnosis of the disease. Some biomarkers are not specific (Rheumatoid Factor IgM) and some are not widely used due to technical problems (Antiprenuclear factor). On the other hand, anti-cyclic citrullinated peptide (anti-CCP) in the serum of patients are more specific for these patients.This move from traditional approaches to use more specific biomarkers for patient stratification and targeted treatment should greatly improve patient care and reduce medical costs.      }, Keywords = {Rheumatoid arthritis, Biomarker, Diagnosis, Prognosis, disease activity}, volume = {9}, Number = {4}, pages = {1682-1692}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-2141-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-2141-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Malekpour, Zahra and Kafilzadeh, Farshi}, title = {Association of IRGM (rs1000113 C/T) Genetic Polymorphism with the Incidence of Acute Rejection in Iranian Liver Transplanted Patients}, abstract ={Background & Objective: Autophagy has been shown to be involved in organ transplantation. IRGM (human immunity-related GTPase) has a crucial role in autophagy complex activation and ROS and microorganism elimination during graft rejection. We examined the association between rs1000113 C/T genetic polymorphism of IRGM and the risk of liver rejection in liver transplanted patients. Materials & Methods: The present study included 100 healthy people and 100 patients with liver disease that led to liver transplantation. Fifty patients were diagnosed with histologically proven acute liver rejection and the other 50 without any rejection. Both groups were matched for sex and age. To determine variants of rs1000113 C/T genetic polymorphism of IRGM, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used. Results: A significant association was observed between liver rejection and rs1000113 C/T genetic polymorphism of IRGM (TC: p-value=0.0098, OR=2.93 CI=1.2-7.22) and (CC: p-value=0.0098, OR=0.34 CI=0.138 -0.83). Also, a significant association was observed between this polymorphism and allelic frequency in liver rejection patients. (T: p-value=0.027, OR=2.14 CI=1.027-4.57) and (C: p-value= 0.027, OR=0.46 CI=0.218 -0.97). No significant difference was found in rs1000113 C/T genetic polymorphism of IRGM, sex, blood group, and underlying disease among the healthy groups and liver transplanted patients. Conclusions: The data suggest that the rs1000113 C/T genetic polymorphism of IRGM, an autophagy-related polymorphic locus, influences liver rejection in liver transplanted patients, with the possible involvement of autophagy in transplantation. Recipients with TC genotype for IRGM are more likely to develop liver rejection compared to those with CC genotype.  }, Keywords = {IRGM, Liver transplantation, Autophagy, Acute rejection}, volume = {9}, Number = {4}, pages = {1693-1702}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-2034-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-2034-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {armand, raham and SadeghiHashjin, Goodarz and Rassouli, ali and Asghari, Mozhg}, title = {The Effects of Nitric Oxide on Blood Coagulation Process}, abstract ={Background & Objective: Nitric oxide (NO) in body can be made of amino acid L-arginine nitric oxide synthase (NOS). Donor drugs that release NO in the body are exogenous sources for this free radical. According to available sources, NO and its precursor (L-Arginine) can affect blood coagulation process. This work investigates the effect of NO on blood coagulation process in vitro using bovine blood as a model. Materials & Methods: Blood samples were taken from 5 apparently healthy adult Holstein cows and were separately exposed to ethylene diamine tetra acetic acid (EDTA) anticoagulation materials and sodium citrate. Then, the blood containing anticoagulation substance was incubated for 30 min at temperature of 37°C with 10, 100 and 1000 µM of donors of NO, including sodium nitroprusside (SNP), nitroglycerine (GTN), isosorbide dinitrate (ISDN). In addition, 1, 10 and 100 mM of substances affecting NOS (either L-Arginine, L-NAME or L-Arginine + L-NAME) were applied in relevant groups. After incubation, following general tests of coagulation were adopted: prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen (FIB), hemoglobin (Hb), platelet count (PLT), red blood cell (RBC) count, mean corpuscular hemoglobin (MCH), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), hematocrit (HCT), white blood cells (WBC) count and methemoglobin (MetHb).  Results: Obtained results indicate that among all relevant works, combination of L-Arginine and L-NAME in applied concentrations can cause significant reduction of red blood cells and hemoglobin concentration (p<0.05). Despite some changes in other parameters, they did not reach the level of significance. Conclusion: In general, it could be mentioned that endogenous or exogenous NO in the model applied in this study may not have a decisive impact on blood coagulation process. However, the non-specific effects observed in the L-Arginine + L-NAME group on the number of red blood cells and hemoglobin concentration need further studies.   }, Keywords = {Nitric oxide, Complete Blood Count (CBC), In Vitro}, volume = {9}, Number = {4}, pages = {1703-1709}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-2096-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-2096-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Pashayi, Pooneh and AbbassiDaloii, Asieh and Barari, Ali Reza and MirJavadi, Syed Rez}, title = {The Effect of Endurance Training and L-Carnitine Supplementation on Gene Expression of Hepatic Enzymes (AST,ALT,ALP) in Wistar Male Rats Toxicated by Boldenone}, abstract ={Background & Objective: The aim of this study was to investigate the effect of endurance training and L-carnitine supplementation on gene expression of hepatic enzymes (AST, ALT, ALP) in Wistar male rats toxicated by Boldenone. Materials & Methods: In this experimental study, 30 male Wistar rats aged 12 weeks (weight 195±7.94g) were randomly divided into five groups: control, no-treatment, boldenone (5mg/kg), L-carnitine and aerobic training- L-carnitine. The endurance moderate intensity training program (50-55% of maximal oxygen consumption) was performed for 6 weeks and 5 times a week. Boldenone injection once a week, on an appointed day, and in the hamstring was conducted in depth. After anesthesia, autopsy was performed and the liver was isolated. The hepatic enzymes gene expression in the samples were measured by Real Time PCR. Data were analyzed by t-test, One-way ANOVA and post hoc Scheffe at the significant level P<0.05. Results: The results showed that there was a significant difference between the mean expression of liver enzymes (AST, ALT, ALP) in male Wistar rats in different groups (P<0.001). The changes in liver enzymes gene expression (AST, ALT, ALP) in L-carnitine and Training-L-carnitine groups were significantly lower than the boldenone group (P<0.001). Conclusion: According to the findings of this study, supplementation of L-carnitine with regular aerobic training reduces liver damage induced by anabolic androgenic steroids.   Keywords: }, Keywords = {Aerobic training, Boldenone, L-carnitine, Hepatic enzymes, Wistar rats}, volume = {9}, Number = {4}, pages = {1710-1718}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-2121-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-2121-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Bijeh, nahid and Farahati, Samaneh and Sarlak, Zahra and Shad, Roghaieh and hooshmandmoghadam, Babak}, title = {The Effect of Eight-Week Aerobic Training on Serum Angiotensin Converting Enzyme and Lipid Profile in Inactive Overweight Women}, abstract ={Background & Objective: At present, the prevalence of hypertension is growing and one of the contributing factors in the occurrence and development of hypertension and atherosclerosis is impaired renin–angiotensin system which in this system endothelium angiotensin converting enzyme is the main enzyme. The aim of this study was to investigate the effect of eight weeks aerobic training on serum angiotensin converting enzyme and lipid profile in inactive overweight women. Materials & Methods: In this semi-experimental study, twenty healthy women (20-30 years old) with BMI>25 kg/m 2 were randomly divided into training group (n = 10) and control group (n = 10). The training group performed aerobic exercise for eight weeks, three sessions per week with 55-75% of maximum heart rate. The duration of each training session was increased from 40 to 60 minutes gradually. 48 hours before and after aerobic training program, anthropometric measurements, VO2max, lipid profile, angiotensin-converting enzyme (ACE) of all subjects were measured. Data were analyzed with paired t-independent test and t-student test at a significance level of P<0.05. Results: Results showed that eight weeks of aerobic training had a significant effect on weight (p=0/01) and BMI (p=0/01) in training group; While changes in angiotensin converting enzyme (p=0/543) and LDL(p=0/927), HDL(p=0/120), Triglyceride (p=0/788) and Cholesterol (p=0/324) during pre-test and post-test was not significant in between groups. Conclusion: Eight weeks of training program have improved angiotensin converting enzyme, LDL, HDL, Triglyceride and Cholesterol in women, approximately. And it can be said that regular exercise may probably have a preventive effect on the prohibition of cardiovascular disease in overweight women.    }, Keywords = {Angiotensin converting enzyme, Lipid profiles, Aerobic training, Inactive overweight women}, volume = {9}, Number = {4}, pages = {1719-1726}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-2077-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-2077-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Dabestan, Masoumeh and Jafarnezhad, Aboubakr and Kashfi, Mansour and Rakhshani, Tayebeh and Gorgi, zeinab and Dehghan, Azizallah}, title = {Emotional Intelligence and Its Relationship with Job Performance in Health Care Providers and Health Workers at Larestan University of Medical Sciences in southern Iran, 2018}, abstract ={Background & Objectives: The present study aimed to determine the level of emotional intelligence and its relationship with job performance in health care providers and health workers of Larestan School of Medical Sciences in 2018. Materials & Methods: This was a cross-sectional study. The Shring questionnaire was used to determine the level of emotional intelligence and its relationship with job performance in health care providers and health workers. Data were analyzed by using SPSS 22 and by Pearson correlation coefficient, one-way ANOVA, Independent Sample T-Test. The significance level was considered 0.05 in all tests. Results: There was a significant relationship between all dimensions of emotional intelligence and performance. Only the self-awareness component directly explained the performance of managers. Conclusions: It is recommended that specific educational programs be implemented in any institute and organization to increase the emotional intelligence of the staff.    }, Keywords = {Emotional, Intelligence, Job performance, health worker, Self-motivation}, volume = {9}, Number = {4}, pages = {1727-1735}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-2150-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-2150-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {morovvati, hassan and armand, nezam}, title = {Assessment of Changes in Serum Concentrations of Liver Function (ALT, ALP, AST, LDH, GGT) After the Intake of Narcissus Bulbs}, abstract ={Background & Objective: Narcissus bulb is an antiphlogistic drug, effective for asthma treatment, shortness of breath and skin burns. The aim of this study was to investigate the effect of Narcissus tazetta bulb extract on serum concentrations of liver function test enzymes (ALT, ALP, AST, LDH, GGT) in male rats. Materials & Methode: In this study, 20 male rats were divided into 4 groups of 5 series. They were divided into control groups and experimental groups and received the alcoholic extract 50, 100 and 150 mg/kg of  Narcissus bulb. The extract was fed for 6 days and blood samples were taken from the animals on the seventh day and serum levels of liver enzymes were measured. Statistical analysis was performed with SPSS software (version 21.0) using t-test. Results: The activity of Aspartate Aminotransferase (AST) and Alanine Aminotransferase (ALT) enzymes in 150 mg/kg extract of Narcissus bulb significantly increased (P<0.01). Also, at the same dose, the enzymes of Alkaline phosphatase (ALP), Lactate Dehydrogenase (LDH) and Gamma Glutamyl Transferase (GGT) showed a significant increase compared to the control group (P<0.05). ALT at 100 - mg/kg dose significantly increased, but AST at 50 mg/kg dose showed a significant decrease compared to the control group (P<0.05).  Conclusion: Considering the significant increase in liver enzymes at high doses of extract, it is necessary to study the histopathological effects of the extracts of this plant on liver.  }, Keywords = {Narcissus, Rat, liver enzyme}, volume = {9}, Number = {4}, pages = {1736-1742}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-2169-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-2169-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {rastgoo, fatemeh and rostaee, zahra and mosleh, fatemeh and Hasannezhad, Arash and GhorbaaniBarnaaji, Hooshmand and abolghazi, ahm}, title = {Study of Soil Contamination by Toxocara Spp. Eggs in Fasa, South of Iran from April to December 2018}, abstract ={Background & Objective: Toxocariasis is a parasitic disease caused by dog ​​ascaris and cat ascaris. Raising pets, especially dogs or cats, has been part of modern life and this raising is the cause of soil contamination by Toxocara spp. eggs. Contacted with contaminated soil is considered the main reservoir of transmission of Toxocara infection to humans. This survey was carried out to clarify the current status of soil contamination by Toxocara spp. eggs.  Materials & Methods: Soil contamination by Toxocara spp. eggs was surveyed in Fasa from April to December 2018. In this study, 56 soil samples were collected from 10 public parks in Fasa. The soil samples were provided by the flotation method and examined by a microscope. Results: The results of this study showed that six (60%) of 10 parks were contaminated by Toxocara eggs. Out of 56 samples collected, 54 Toxocara spp eggs were found. Conclusion: High prevalence of Toxocara spp. eggs in soil samples of this study can be associated with the rising population of stray dogs or cats and pets in public parks, the carriers of adult worms and the active source of soil contamination.    }, Keywords = {Toxocara, Fasa, Soil, Contamination}, volume = {9}, Number = {4}, pages = {1743-1748}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-2055-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-2055-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {AlipoorBirgani, Anahita and Sartipnia, Nasrin and Hamdi, Seyed Mohammad Mahdi and Naghzadeh, Mohammad and Arasteh, Jav}, title = {Antimicrobial Activity of Scabiosa Olivieri Extract and Its Effect on TNF-α and IL-1 Expression in Human Peripheral Blood Cells (PBMCs)}, abstract ={Background & Objectives: Infectious diseases caused by bacteria and fungi have affected billions of people worldwide. Throughout human history, infectious diseases have been the leading cause of death and disability. Infectious diseases today account for one-third of all deaths in the world. The general objective of this study was to investigate the antimicrobial activity of Scabiosa Olivieri on gram-positive, gram-negative bacteria and fungi, as well as to study its anti-inflammatory properties by investigating the factors of human IL-1 and TNF-α, which ultimately led to the introduction of an antimicrobial agent and new anti-inflammatory drugs with a natural and inexpensive source. Materials & Methods: First, the extract of the plant was prepared by maceration. Then, the antimicrobial properties of this extract on E. coli, Staphylococcus aureus, and Candida albicans were investigated by MIC. Also, the effect of this extract on the expression of IL-1 and TNFα in human peripheral blood mononuclear cells was evaluated by ELISA. Results: Scabiosa Olivieri's extract significantly showed anti-inflammatory properties and has antimicrobial activity against Escherichia coli and a mild antimicrobial activity against Staphylococcus aureus. But the antifungal property was not observed. Conclusion: It seems that Scabiosa Olivieri's extract can be used as an anti-inflammatory and antimicrobial agent. However, the effects of its use in the in vivo environment and the chemical analysis of its constituent compounds require further research.}, Keywords = {Scabiosa Olivieri, PBMC, anti-inflammatory effect, antimicrobial activity, MIC, IL-1, TNF-α}, volume = {9}, Number = {4}, pages = {1749-1757}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-2171-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-2171-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {ZareiTaher, Tahereh and Kowsari, Ali and Sheykhhasan, Mohsen and Manoochehri, Hamed and Kalhor, Naser and Naserpour, Leil}, title = {Introduce Characteristic, Types and Functional Mechanism of Adjuvants as a Biologic Complement}, abstract ={The widespread use of effective vaccines against infectious diseases has been one of the most important public health progresses in the 21st century. Early vaccines containing weakened or inactivated pathogens or toxins may elicit robust, protective immune responses, but this approach cannot always be used because it is impractical and inefficient to culture large numbers of organisms, and lack of safety. In such cases subunits (e.g., microbial proteins or carbohydrate) are being considered as vaccine antigens. Since the subtypes of antigens often have weak immunogenic properties, they cannot stimulate the innate immune system. This property is probably the cause of the reduced efficacy of the vaccine. Adjuvants are molecules, compounds, or supramolecular complexes that boost the potency and longevity of specific immune response to antigens, but can cause minimal toxicity or long-lasting immune effects on human. Adjuvants can be used to enhance immunogenicity, modulate the type of immune response, reduce the amount of antigen or the number of immunizations required for efficacy, and/or improve the efficacy of vaccines in specific populations (e.g., newborns or elderly). The present paper reviews the different types of adjuvants and examines the characteristics and mechanisms of their performance.  }, Keywords = {adjuvants, characteristics, mechanism, function}, volume = {9}, Number = {4}, pages = {1758-1773}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1772-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1772-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Rahimian, Shohreh and AminYazdi, Seyyed Amir and Bigdeli, Imanollah and Edalatmanesh, Mohammad Ami}, title = {Intense World Theory: New Perspective in Brain\'s Function and the Role of Amygdala in Autism Spectrum Disorder}, abstract ={Background & Objective: In Autism Spectrum Disorder, the role of functional deficit like hypo-functionality in Frontal lobe, Hippocampus, Amygdala with top-down control hypothesis or reduced functional connectivity has been considered by theories like theory of mind or executive function theory. But new findings in the fields of neurobiology and neuroscience have challenged these theories and these lead to a new theory that was introduced by Hennry Markram in 2007. This theory is presented as "Intense World Theory". Markram believes that hyper-functionality, hyper-reactivity and Hyper-plasticity in brain's microcircuits with a down-up pathway is the basis of Autism. Conclusion: In this study various aspects of intense world theory, empirical evidence, and challenges and criticisms of this theory are discussed. In addition to creating a new research scope and paying attention to the underlying mechanisms of the brain, this review may lead to early diagnosis of disorder and effective therapeutic procedure in the medical, psychological and other fields.  }, Keywords = {Autism Spectrum Disorder, Intense World Theory, Hyper-Functionality, Amygdala Inhibition Deficit, VPA Animal Model}, volume = {9}, Number = {4}, pages = {1774-1783}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-2035-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-2035-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {behjatimoghaddam, mahsa and neamati, ali and ardalan, pour}, title = {Evaluation of the ZnO Nanoparticles Biosynthesized by Amaranthus Ruentus on the Expression of Apoptotic Genes (Bax and Bcl-2) in Breast Cancer Cells (MDA-MB-231)}, abstract ={ Background & Objective: Cancer is a kind of genetic disease caused by DNA mutation which makes disorder in normal pattern of division and differentiation of cells and eventually formation of a neoplasm. Breast cancer is the most common malignancy and the second leading cause of death in women aged 35-55 years. Surgery, radiotherapy and chemotherapy are the common methods of controlling cancer, but the side effects and lack of positive results especially in metastatic tumors has led us discover new treatments. Nowadays, nanotechnology has helped us to find and develop new therapies. The purpose of this study was to characterize the antioxidant and apoptotic properties of zinc oxide nanoparticles biosynthesized by Amaranthus cruentus plant on breast cancer cells (MDA-MB-231). Materials & Methods: The Zinc oxide nanoparticles were green-synthesized by the extract of Amaranthus cruentus leaves. Size and morphological characteristics of ZnO NPs determined by DLS tests, FESEM and TEM. To investigate the effect of ZnO nanoparticles on induction apoptosis, cancer cells were seeded in T25 flasks and treated with different concentrations of nanoparticles (15, 30 and 60 μg/ml). After 48 hours, gene expression changes of Bax and Bcl-2 was investigated by Real time PCR technique. SPSS software and one-way ANOVA test were used to analyze the data. At the end, comparison of means did by least significant differences (LSD) method. Results: The DLS test showed the average size of the synthesized ZnO NPs is about 30 to 38 nm. The results of gene expression by Real time PCR technique showed that ZnO nanoparticles reduced anti-apoptosis gene expression Bcl-2 and increases pro-apoptotic gene expression in MDA cell line. Conclusion: In general, the results obtained from this study can claim that ZnO nanoparticles have anti-cancer properties and can be introduced after further studies as candidates for cancer treatment in the field of medicine and pharmacy.  }, Keywords = {Zinc oxide, Nanoparticle, Gene expression, Apoptosis, Breast Cancer}, volume = {9}, Number = {4}, pages = {1784-1793}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1913-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1913-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {hooshmand, elaheh and nejatzadegan, zohre and ebrahimipour, hossein and esmaily, habibollah and vafaeenajar, ali}, title = {The Challenges of the Family Physician Program in the North East of Iran from the Perspective of Managers and Practitioners Working on the Plan}, abstract ={Background & Objective: In Iran, a new program called the Family Doctor was started in 1384. The present study aims to determine the challenges of the family physician system in Khorasan Razavi province. Materials & Methods: The present study is a quantitative-qualitative study. In this study, a sample-based sampling method was conducted with 11 managers and 9 family physicians working in the project in Khorasan Razavi. Then, based on the results of the interviews, a questionnaire was designed and confirmed by its validity and reliability in a descriptive-analytic study in 1395. The findings of the quantitative study were analyzed using descriptive statistics and Mann-Whitney, T-test and ANOVA tests at a significant level of 0.05 Results: In the analysis of qualitative content 9 basic concepts (comprehensive planning, health insurance system, compensation services, performance appraisal, facilities and welfare facilities, recruitment and maintenance of human resources, information system, culture and financing) were identified. The biggest challenge is in the area of compensation (4.55 ± 0.73) and the smallest in the area of facilities and facilities (3.67 ± 0.83). The greatest difference is between the viewpoints of managers and physicians in the field of facilities and amenities (0/61 ± 0/20) and the least in the field of culture (0.04 ± 0/17). Conclusion: The most common challenges faced by family physicians in the study are cultural, economic, environmental and working conditions. It is suggested that, in order to properly implement the plan of the family physician, it should be considered in coordination with the objectives of the plan at international level and the experiences of the countries that implemented the program.  }, Keywords = {Rural family Physician, Primary health care, First level of service provision}, volume = {9}, Number = {4}, pages = {1794-1808}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1530-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1530-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Ghorbani, Parisa and Beyramabadi, S. Ali and Homayouni-Tabrizi, Masoud and Yaghmaei, Parichehreh}, title = {The Study of Antiangiogenic Effects of Oxovanadium(IV) Complex of N,N′-Dipyridoxyl(1,2-cyclopropanediamine) Schiff Base on the Chorioallantoic Membrane of Chick Embryos Cells}, abstract ={Background & Objective: Schiff bases are considered as an important group of compounds in the development of new drugs. The current approach to finding more effective treatment for cancer is to set up different stages of the angiogenesis process using angiogenesis suppressors, such as metal complexes synthesized from the Schiff bases. Therefore, the aim of this research was to investigate the antiangiogenic effects of oxovanadium(IV) complex of N,N′-dipyridoxyl(1,2-cyclopropanediamine) Schiff base on the chorioallantoic membrane of chick embryos cells. Materials & methods: Anti-angiogenic activity of oxovanadium(IV) complex of N,N′-dipyridoxyl(1,2-cyclopropanediamine) Schiff base was evaluated using Real-time PCR technique to evaluate the expression changes in angiogenesis-related genes in MCF-7 breast cancer cells and CAM test. Results: The study of alterations in the expression of genes showed that oxovanadium(IV) complex of N,N′-dipyridoxyl(1,2-cyclopropanediamine) Schiff base had a significant inhibitory effect on the expression of both genes involved in angiogenesis. The findings of the CAM test showed that there was a significant decrease in the number and length of the vessels treated with different doses of the complex (100, 200, 400 and 800 μg/mL) (P <0.01 and P <0.001). Also, there was a significant decrease in weight and height of embryos treated with complex compared to control group at P <0.05, P <0.01 and P <0.001. Conclusion: In this study, the oxovanadium(IV) complex of N,N′-dipyridoxyl(1,2-cyclopropanediamine) Schiff base has antiangiogenesis effects that make the use of this complex possible for cancer treatment.}, Keywords = {Oxovanadium(IV) complex, N,N′-dipyridoxyl Schiff base, Antiangiogenesis, Chorioallantoic membrane}, volume = {9}, Number = {4}, pages = {1809-1818}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1934-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1934-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {movahed, majid and majidi, maryam}, title = {Active Aging Index and Related Factors Among Aged People in Shiraz in 1397}, abstract ={Background & Objective: Aging is one of the important stage in human life. Nowadays world society regards that matter as an important problem. This study aimed at measuring Active Aging Index in Shiraz and its related and affected factors. Materials & Methods: This survey has been done by quantitative method among a sample of Shirazi elders involving 600 cases. Sampling method was hierarchal cluster sampling. Data were collected by questionnaire and interview. Variables were context information, living arrangement, active ageing, social support, mental health, spiritual health and self-efficacy. Results: Mean of AAI was 51.01± 15.2 (from 100). Social support was the most effective factor on AAI and then education, spiritual health, self-efficacy, mental health, age and income be effected respectively. Consequently, those factors explained over 70% of Active Aging variation.  Conclusion: Elders in Shiraz have median activity number. This number of activity is not acceptable for development. Iran population is increasing rapidly. We have to work on this problem more and more. Attention to social support, education and awareness can be very effective in this situation.    }, Keywords = {Active Aging, Spiritual Health, Mental Health, Social Support, Self-efficacy}, volume = {9}, Number = {4}, pages = {1819-1828}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1895-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1895-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Asghari, Narjes and Zarrinnpour, Vajiheh and Daraei, Abdolreza and Hajebrahimi, Zahra and Moslemi, Dariush}, title = {The Evaluation of VE-Cadherin Gene Expression in Serum of Iranian Women With Breast Cancer}, abstract ={Background & Objective: Breast cancer is one of the most common cancers and the second leading cause of cancer death in women worldwide, in both developed and developing countries.  Vascular endothelial (VE)-cadherin is endothelial specific adhesion molecule located at junctions between endothelial cells and its association with various cancers has been proven. The aim of this study was to evaluate the VE-cadherin gene expression in serum of Iranian women with breast cancer in order to identify a biomarker for its prediction. Materials & Methods: After serum isolation from blood samples of the patients and controls, extraction of RNA and synthesis of cDNA were done. Gene expression was measured using real-time PCR technique. Results: Our results showed that the expression of VE-cadherin was increased significantly in patients compared to control samples. However, there was no relationship between its expression and grade of the tumors. Conclusion: Based on the results of this study, expression of this factor can be used as a marker for study and follow-up of breast cancer patients. Furthermore, considering the role of this factor in angiogenesis and metastasis, it can be used as a target for intervention therapy.  }, Keywords = {breast cancer, evaluation of gene expression, VE-cadherin gene, serum biomarker}, volume = {9}, Number = {4}, pages = {1829-1836}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-2010-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-2010-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {nameni, farah and nuranipilehrud, maasumeh and mohsenzadeh, mahsa and aghaii, farib}, title = {The Effect of Short Term Supplementation with Curcumin on Superoxide Dismutase, Glutathione Redoctase, Glutathione Peroxidase and Catalase Serum in Basketball Players}, abstract ={Background & Objective: Curcumin has antioxidants effects and strong anti-inflammatory properties and has a protective and therapeutic roll. This combination can be effective in reducing inflammation and increases reactive oxygen species (ROS) in the skeletal muscle and blood. This study aimed at investigating the effect of supplementation with curcumin on antioxidants enzymes after a basketball intensive training session in girls. Materials & Methods: For this purpose 30 female basketball players are divided into 2 homogeneous supplement and placebo groups. Oral curcumin was used for a period of 14 days,3 time a day in the amount of 1200 mg by the supplement and placebo groups. After 14 days of supplementation, all subjects participated in the basketball acute training protocol. Blood samples were collected and used for determination of antioxidant enzymes activity on base, after supplementation and after exercise training. Differences between the placebo and curcumin groups were analyzed by analysis of variance (ANOVA) repeated measures test and Bonferroni test was used for post hoc multiple comparisons among means. Results: Mean± SD showed all antioxidant enzymes increased but ANOVA repeated measures results showed that GPX and SOD activity (U/mg pro) in supplemental group was increased significantly (P=0.05); Whereas, CAT and GR activity (U/mg pro) were not significantly different. Conclusion: These findings showed that curcumin consumption before one bout acute training may affect antioxidant enzymes concentration and promotes antioxidant capacity of adolescent athletes during heavy competitions. This strategy can accelerate recovery from repeated stress. Curcumin increased antioxidant activities.    }, Keywords = {Curcumin, anti-oxidant enzymes, basketball players, supplementation}, volume = {9}, Number = {4}, pages = {1837-1847}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1606-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1606-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Honarvar, Behnam and Qaidipour, Nezam and Maghsoudi, Fatemeh and Rostami, Sara and Salehi, Forough}, title = {Observance of Hospital Rules and Nurses Rights by Patients\' Companions After Health System Reform: Results of Hospital Based Study in Shiraz, Iran}, abstract ={Background & Objective: Health system reform confronts hospitals with a lot of patients and their companions. This study aimed to determine the knowledge, attitude and practice of patients’ companions toward hospitals rules and nurses’ rights. Material & Methods: This descriptive-analytic cross-sectional study was conducted through a multistage cluster random sampling of patients’ first degree relatives who had accompanied their patients in main public hospitals of Shiraz, Iran. Valid and reliable questionnaire was filled for each interviewee by a face to face interview. Data were analyzed using SPSS. Results: Mean age of 423 interviewees was 36.7±11.7 years with female to male ratio of 1.29. 292(67.4%) were educated until the end of high school, while 264(61.1%) had a kind of job. Knowledge, attitude and practice toward hospitals’ rules and nurses’ rights were inappropriate in 61(14.1%), 84(19.4%) and 221(51.2%) respectively. Moreover, Sum score of these variables was inappropriate in 102(23.6%). Correlation between knowledge and practice was 0.44(p<0.001). Total score of knowledge, attitude and practice had a significant association with the kind of hospital, gender, level of education, marital status, being bread-winner of family, having a job, patients’ finance providing, cohabitation with patient in the same place, admission ward of patient, last time of interviewee’s admission in hospital and amount and source of information about hospitals rules and nurses’ rights. Conclusion: Inappropriate practice of patients’ companions toward hospitals rules is associated with violence against nurses, their burnout and decreasing quality of hospital services. Therefore, following health system reform in Iran and increasing trend of referrals to hospitals, efficient interventions to improve this index is recommended.  }, Keywords = {Rule, Hospital, Right, Nurse, Health System}, volume = {9}, Number = {4}, pages = {1848-1857}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1897-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1897-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {Rezaii, Somaye and Ghassemi, Farangis}, title = {The Effect of Aqueous Fruit Extract Berberis Vulgaris on The Liver and kidney Tissues of Rat’s Embryo}, abstract ={Background & Objective: Considering the undesirable effects of eating some foods during pregnancy, this study investigated the effect of aqueous fruit extract of Berberis vulgarison on the development of liver and kidney. Materials & Methods: 56 female rats (188 ± 20 gram) and 8 male rats (188 ± 20 gram) were selected and fertilized. The pregnant rats were divided into the control group (without treatment), sham (received physiological serum) and 2 treatment groups and received 0.2cc of aqueous extract of Berberis vulgaris at doses of 20 and 50 mg/kg/bw ) by intraperitoneal injection daily from the 6th - 10th day of gestation. Half of the rats were dissected on 14thand 20thof pregnancy. The kidneys and livers of embryos were removed and fixed in 10% formalin. The sections (5 micron) and slides were prepared and stained. The morphometric study was conducted, and tissue changes in the micrographs were studied. The data were analyzed by ANOVA and Chi-squared test. Results: The significant decrease in height (P<0.01), and significant increase in weight of treatment fetus (20-day-old) were observed. The liver tissue of treatment 20-day-old fetus showed sinusoid hyperemia, vascular endothelium degeneration, reduction in the number of hepatocytes and their vacuolation. The moderate changes were observed in kidney tissue as degradation of the duct epithelium, in the 20-day-old embryos. Conclusion: According to obtained finding, Barberry has negative effects in a dose- and time-dependent manner on the liver, and somewhat on kidney of fetus. Therefore, its utilization should be limited during pregnancy.    }, Keywords = {Embryo, barberry, hepatocyte, urinary tract}, volume = {9}, Number = {4}, pages = {1858-1869}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1549-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1549-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {sadeghi, sahar and sadeghisedeh, bahman and mirzaee, hoshang and rasafiani, mehdi and pishyareh, ebrahim}, title = {Translating and Standardizing the Persian Version Measure of Process of Care for Service Providers (MPOC-SP) in Down Syndrome}, abstract ={Background & Objective: Down syndrome is the most common type of chromosomal abnormality that causes mental disability. In recent years, the life expectancy of these children has increased, so their special needs will also increase. Family-centered interventions are of particular importance for the provision of services to children and their families. There are several tools for measuring family-centered services from the perspective of parents and service providers. But the Persian version of validity and reliability is not found in information resources. Therefore, the aim of this study was to translate and standardize the Persian version of the questionnaire for measure of process of care for service providers (MPOC-SP) in children with Down Syndrome in Isfahan. Materials & Methods: The original version of the Measure of process of care for service providers (MPOC-SP) in a cross-sectional study was translated in three stages. A final version of Persian was provided. Experts' opinions and Kappa agreement coefficient were used to examine the face and content validity. To determine the reliability of the Persian version, by random sampling, 83 occupational therapists, general practitioners and psychiatrists were selected and test-retest was administered. Finally, the data were analyzed using SPSS 19 software, Cronbach's alpha and intra-cluster correlation coefficient, and multi factors analysis. Results: The internal consistency of the four domains of the questionnaire was obtained using Cronbach's alpha between 0.778 and 0.881. The reliability values ​​were within the cluster correlation index between 0.75 and 0.83. The correlation between the four domains was favorable on the basis of Spearman correlation coefficient. The correlation of each of the questions with the four fields of the questionnaire in multi factors analysis (0.72-0.87) was also appropriate Conclusion: The Persian version of measure of process of care for service providers in children with Down Syndrome with 27 items and in four different areas has a good validity and reliability as a tool for evaluating the provision of services in all centers for providing services to children with Down Syndrome.  }, Keywords = {Down Syndrome. Measure of process of care for service providers (MPOC-SP) Questionnaire. Translation and validation}, volume = {9}, Number = {4}, pages = {1870-1878}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1793-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1793-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {TahmasebiFard, zahr}, title = {The Effect of the Polymorphism on the 5\' Region of the Leptin gene (G2548A) on the Possibility of Breast Cancer in two Studies Groups}, abstract ={ Background & Objective: Leptin a hormone secreted from adipose tissue plays a role in regulating energy homeostasis. Polymorphism in the gene structure can alter serum levels of hormone and affect cell function. In this study, the relationship between G2548A polymorphism of the leptin gene with the risk of breast cancer in patients compared with healthy subjects was investigated. Materials and Methods: The blood sample of 158 women with breast cancer and 158 healthy women of the same age was collected and after extracting DNA and amplifying specimens with specific primers, some part of products was digested with HhaI restriction enzyme to determine the genotypes of individuals based on the number of bands formed on the gel. The results were analyzed by statistical analysis including chi-square, and logistic regression. Results: The mean age of the patients was 56.00±0.62 years old and the control groups was 55.45±0.72 years old (p-value =0.037). There was a significant relationship between the smoking level of the two groups (p-value =0.026). The history of relatives of breast cancer patients was positive in 43 (27.22%) patients. Analysis of the results of genotypes showed that the mutant homozygote genotype AA had a significant relationship in two groups and increased by 1.686 the risk of breast cancer (P-Value=0.036, OR:1.686, CI95%:1.033-2.753). Conclusion: The frequency of mutant A allele in the patient group was more than that of the control groups and increased the risk of breast cancer by 1.763 times in carriers. Overall, it can be said that this polymorphism is probably related to breast cancer.    }, Keywords = {Breast Cancer (BC), Leptin, single nucleotide polymorphism, Restriction Fragment Length Polymorphism (RFLP)}, volume = {9}, Number = {4}, pages = {1879-1885}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-2006-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-2006-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {alimoradi, mahjoobeh and didehdar, soosan and zolfaghari, hamid and mostafapour, vahid and farrokhi, hossei}, title = {The Effectiveness of Mindfulness-Based Cognitive Therapy Group in Decreasing Depression and Increasing Quality of Life in Women with Addicted Husbands}, abstract ={Background & Objective: Addiction as a disease is a disadvantage that affects the quality of life of families and couples and may cause depression among other members of the family. The purpose of this study was to investigate the effectiveness of Mindfulness-Based Cognitive Therapy Group in decreasing depression and increasing the quality of life in women with addicted husbands. Material & Methods: The semi-experimental design was pre-test-posttest with control group. The statistical population included all women with addicted husbands referring to the comprehensive health center of Fasa into the experimental and control groups (15 women in each group). The experimental group received 8 sessions of mindfulness-based cognitive-group therapy, and no intervention was provided to the control group members. The research instrument questionnaire was Beck and Quality of Life. Results: The results showed that mindfulness-based cognitive-group therapy was effective in depression and quality of life of women with addicted husbands (P<0.001) and treatment reduced depression and increased the quality of life of women in post-test of the experimental group. Conclusion: The results show that Mindfulness-Based Cognitive Therapy Group is effective in decreasing depression and, consequently, increasing the quality of life of addicted spouse.  }, Keywords = {Mindfulness, Depression, Quality of Life, Women with Addicted Husband}, volume = {9}, Number = {4}, pages = {1886-1895}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1861-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1861-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {shahraki, Iman and Jamshidian, abbas and Hajinezhad, Mohammad Reza and Akbari, mohammad Ebrahim and davari, ai}, title = {Effects of Averrhoa Carambola Hydro-Alcoholic Extract on Liver Histopathological Changes in Rats with a High-Fat Diet}, abstract ={ Background & Objective: In the present study the effects of Averrhoa carambola fruit hydro-alcoholic extract on hepatic tissue, liver enzymes, and serum lipids were investigated in rats submitted to a high-fat diet. Materials & Methods: In this experimental study, 30 male Wistar rats were randomly divided to 3 equal groups as follows: (1) control, (2) rats fed a high-fat diet and (3) rats receiving a high fat diet and hydro-alcoholic extract of Averrhoa carambola (A. carambola) at a dose of (200 mg/kg bw). Rats received a high-fat diet for one month. Then, two rats were selected from each diet group and hyperlipidemia was confirmed by measurement of serum triglyceride and serum cholesterol levels. After confirming hyperlipidemia, rats of group 3 were orally treated by Averrhoa carambola (200 mg/kg bw) for 21 days. At the end of the experiment, serum liver enzymes, serum cholesterol and triglyceride levels were assayed. Afterward, for histopathological investigation, hepatic tissue samples were prepared and routine tissue processing protocol was performed. Results: The administration of Averrhoa carambola fruit extract significantly reduced serum triglyceride and cholesterol when compared with the hyperlipidemic group (P < 0.05 and P < 0.001 respectively). Serum ALT and AST in rats that received Averrhoa carambola fruit extract significantly decreased compared to hyperlipidemic group (P< 0.05). In the histopathological examination, degeneration, vacuolation and intra-cytoplasmic accumulations in the treated group were improved in comparison with the hyperlipidemic group. Conclusion: These results showed that the Averrhoa carambola fruit extract can protect against hyperlipidemia- induced liver damage in rats.  }, Keywords = {Hyperlipidemia, Liver, Averrhoa carambola, Rats}, volume = {9}, Number = {4}, pages = {1896-1902}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1955-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1955-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} } @article{ author = {adyani, pooran and Abbassi-Daloii, Asieh and shadmehri, saeedeh}, title = {The Effect of Endurance Training and L-Carnitine Consumption on TNF-a and IL-1β Gene Expression of Heart Tissue in Wistar Male Rats following Anabolic Steroid Consumption (Boldenone)}, abstract ={ Background & Objective: The aim of this study was to investigate the effect of endurance training and L-carnitine consumption on TNF-a and IL-1β gene expression of heart tissue in Wistar male rats following anabolic steroid consumption (Boldenone). Materials & Methods: In this experimental study, 30 male Wistar rats aged 12 weeks (weight 195±7.94g) were randomly divided into five groups including control, no-treatment, boldenone (5mg/kg), L-carnitine and aerobic training- L-carnitine. The endurance moderate intensity training program (55-50% of maximal oxygen consumption) was performed for 6 weeks and 5 times a week. Injection was conducted once a week, on an appointed day, and in the quadriceps and hamstring it was conducted in depth. After anesthesia, autopsy was performed and the heart was isolated. The TNF-α and IL-1β gene expression in the samples was measured by Real Time PCR. Data were analyzed using t-test, One-way ANOVA and post hoc Scheffe at the significant level of P<0.05. Results: The results showed that there was a significant difference between the mean TNF-α and IL-1β gene expression of heart tissue in male Wistar rats in different groups (P=0.001). The changes in TNF-α and IL-1β gene expression of heart tissue in L-carnitine and Training-L-carnitine groups were significantly lower than those of the no-treatment and boldenon groups (P=0.001). Conclusion: It seems that supplementation of L-carnitine with regular aerobic training reduces heart tissue damage induced by anabolic androgenic steroids.  }, Keywords = {Aerobic training, Boldenone, L-carnitine, TNF-α, IL-1β}, volume = {9}, Number = {4}, pages = {1903-1912}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1911-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1911-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2019} }