@article{ author = {KarbalaieNiya, Mohammad Hadi and Tavakoli, Ahmad and Foroughi-Nia, Behrouz and SafarnezhadTameshkel, Fahimeh and Esghaei, Maryam}, title = {Roles of Integrin and Its Application for Anti-viral Drug Development}, abstract ={Integrins are a large family of adhesion molecules under cellular control that could act bilabially in different situations; on the other hand, they play a significant role in adsorption and entry of immune system cells or other helper cells. Furthermore, they could be good targets for entry, localization and replication of infectious viruses into cells. As viruses apply various strategies for entry and infiltration to cells, comparison of these ways (especially integrin mediated), elucidates effective mechanisms in the inception of viral infection and the host cells interactions. At this point, the present study reviewed the relationships between common viruses such as Adenovirus, Papillomavirus, Herpesvirus, Hantavirus, Rotavirus, Echovirus, foot-and-mouth disease virus, Coxsackievirus type 9, Parechovirus type 1 and Human immunodeficiency virus type 1 with integrins and their viable interactions for therapeutical issues and better recognition of the commencement process of the infection by these viruses.}, Keywords = {Integrin, Virus, Receptor, Anti-viral Drug}, volume = {8}, Number = {1}, pages = {582-593}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1237-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1237-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {MohamadRezaii, Hossein and Kalantari, Mohsen and Mahmazi, Sanaz}, title = {Association of UCP3 (uncoupling protein 3) gene -55C/T polymorphism and obesity in a juvenile population of Iran}, abstract ={Background & Objective: UCP3 is a member of uncoupling proteins which plays an important role in energy homeostasis and it might be important in obesity. C-55T (rs1800849) polymorphism in promoter of UCP3 can affect the expression level of UCP3. The aim of this study was to analyze the association of UCP3 C-55T polymorphism and obesity in a juvenile population of Iran. Material & Methods: peripheral blood sample from 120 obese and 120 normal university students were collected for DNA extraction. Genotyping of C-55T polymorphism in the UCP3 gene was performed by PCR-RFLP method. Chi-square test was applied to analyze the data at P<0.05 significance level. Results: The variant T allele was significantly associated with the obesity risk. The frequency of T allele was 72% in Obese group compared to 40% in normal subjects (P= 0.000, OR=3.8 CI 95% 2.1 – 6.9). Conclusion: The UCP3 C-55T polymorphism was associated with higher BMI and can be regarded as a risk factor for the development of obesity.    }, Keywords = {obesity, UCP3 gene, polymorphism, PCR-RFLP}, volume = {8}, Number = {1}, pages = {594-600}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1089-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1089-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Najmikargan, Behzad and KargariRezapour, Ali and Sharifi, Rasoul}, title = {The Effects of Levels of Oral Di-(2-ethylhexyl) Phthalate on Glycemic Response in Mice}, abstract ={Background & Objective: Humans are widely exposed to di-(2-ethylhexyl) phthalate that it can rise obesity and type 2 diabetes epidemics. The object of this study was to investigate the effects of oral di-(2-ethylhexyl) phthalate (DEHP) at minimum and maximum levels on glycemic response in mice. Material & Methods: An interventional study with 15 adult male mice was designed. Mice were randomly assigned to 3 equal groups: control, 1x phthalate (dose of 250 mg/kg/day), 2x phthalate (dose of 500 mg/kg/day) and treated for a period of 8 weeks. At the end of the seventh week study all mice were tested by oral glucose tolerance test and area under curve (AUC) was calculated for each sample. In the end, fasting blood samples were used to measure the variables (Glucose, Insulin, HOMA_IR, Quicki of serum and HbA1C of total blood). The data were analyzed by ANOVA and Duncan tests. The significant difference in the data was considered P >0.01. Results: Di-(2-ethylhexyl) phthalate at low dose (250 mg/kg) showed significant increase in serum glucose and insulin resistance levels and also quicki levels decreased significantly compared to other groups (P <0.01). This study showed no significant changes in insulin serum, glycated hemoglobin (HbA1c) of total blood and area under the curve in glucose tolerance test (P >0.01). Conclusion: In the present study, it showed di-(2-ethylhexyl) phthalate at low dose (250 mg/kg) impaired blood sugar control systems and this can lead in pre-diabetes.    }, Keywords = {Di-(2-ethylhexyl) phthalate, Glycemic response, Mice}, volume = {8}, Number = {1}, pages = {601-607}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1175-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1175-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Chekachak, Soodabeh and MolanouriShamsi, Mahdieh and Soudi, Sar}, title = {Investigating The Effect of Aerobic Interval Training with Selenium Nanoparticles on the Content of IL-6, TNF-α and IL-4 cytokines in spleen tissue of Mice with Breast Cancer}, abstract ={Background & Objective: Cytokines are molecules that are related to immunologic responses. Considering the antioxidant and immunologic effects of selenium nanoparticles and aerobic exercise training, the aim of this study was to examine the effects of aerobic exercise training and supplementation with selenium nanoparticles on protein content of Interleukin-4, Interleukin-6 and tumor necrosis factor Alpha in spleen tissue in mice with breast cancer. Material and Methods: 64 Balb/c mice were randomly divided into 8 groups including Control-Health, Control-Tumor, Training-Healthy, Training-Tumor, Selenium nanoparticles-Health, Selenium nanoparticles-Tumor, Selenium nanoparticles-Training-Health and Selenium nanoparticles–Training-Tumor. Animals in different groups passed 6 weeks aerobic interval training and supplementation with selenium nanoparticles before and after cancer induction. At the end of the sixth week, cytokines protein levels in spleen tissue were measured by ELISA method. Results: Based on the current study results exercise training and selenium nanoparticles caused significant decrease in tumor volume (P<0.05). Also, results showed that cancer induced a significant decrease in cytokines levels in tumor tissue (P<0.05). But concurrent using selenium nanoparticles and aerobic interval training induced increase in tumor necrosis factor Alpha levels in spleen tissue (P<0.05). Conclusion: It seems that aerobic exercise training and selenium nanoparticles supplementation could have an effective role in the activation of immune system and decreasing in tumor volume, with increasing in tumor necrosis factor Alpha as a T helper 1 cytokine.}, Keywords = {Aerobic training, Selenium nanoparticles, Cancer, Cytokine, Spleen}, volume = {8}, Number = {1}, pages = {608-617}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1315-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1315-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Ghaffari, Shima and Asadzadeh, Azizeh and SeyedhosseiniGhaheh, Horia and Sholehvar, Fatemeh}, title = {Docking Study on salicylaldehyde Derivatives as Anti-melanogenesis Agents}, abstract ={Background & Objective: Abnormal production of melanin pigment which causes melasma, freckles, ephelides, and age spots, are esthetic problems. Polyphenol oxidase (PPO), a copper-containing enzyme, is involved in melanin biosynthesis and the abnormal accumulation of melanin pigments. Thus, its inhibitors are of great importance in the medical and cosmetic fields. The aim of this study was to investigate some salicylaldehyde Analogues as Polyphenol oxidase inhibitors. Material & Methods: In the present study, thirty five derivatives of salicylaldehyde scaffold were subjected to molecular docking studies to investigate the mode of interaction of the compounds with tyrosinase active site. Docking study was performed by AutoDock 4.2 program and the resulting docking poses were analyzed in AutoDockTools, DS Visualizer 3.5 and Ligplot softwares. Results: Among the all studied compounds, Ligand 4-isopropylsalicylaldehyde displayed good docking results. In fact, this compound had the most negative ΔGbind (-4.01 Kcal/mol) that indicated favorable interactions with the key amino acid residues at active site of Polyphenol oxidase. Docking results for this compound are in accordance with the docking results of Co-crystallized ligand (tropolone). In this compound, the oxygen of a carbonyl group has an efficient metal-ligand interaction with the Cu2+ ion in the active site. Conclusion: The presence of non-polar moiety in salicylaldehyde Analogues increases the inhibitory property.  }, Keywords = {docking study, salicylaldehyde derivatives, Polyphenol oxidase, Anti-melanogenesis}, volume = {8}, Number = {1}, pages = {618-627}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1395-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1395-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Naeimi, Sirous}, title = {Investigating CpG islands methylation in P16/Ink4 gene promoter regions and the effect of Interleukin-17 gene polymorphism on this methylation in patients with breast cancer}, abstract ={Background & objective: Studies have shown that increased methylation of CpG islands is one of the important mechanisms in gene silencing. Protein P16 / Ink4 plays an important role in the negative regulator of cell cycle process. Inflammation, including factors that affect gene methylation and IL-17, as an inflammatory cytokine, can play a role in this case. This cytokine gene has several polymorphisms which are involved in the expression of it. According to the statement, the purpose of this study is to investigate IL-17 gene polymorphism on gene promoter methylation of P16 / Ink4 and its relation to breast cancer diseases.   Material & Methods: In this case - control study, a total of 40 Women with Breast cancer and 40 healthy women on September 2015 were examined. DNA was extracted and for gene promoter methylation, MSPCR method was used. Single nucleotide Polymorphisms of the IL-17 gene were analyzed by the PCR-RFLP method. Data were compared in both groups by using Pearson’s chi-square and Hardy-Weinberg equilibrium test. Results: Results confirm the fact that, there is a relationship between P16/Ink4 gene promoter methylation and breast cancer disease So that, the promoter of P16/Ink4 gene in healthy individuals was much more unmethylated than patients (p<0.05). On the other hand there is no significant difference between IL-17 gene polymorphisms and DAP-kinase gene methylation (P>0.05). Conclusion: It seems that increases of P16/Ink4 gene promoter unmethylation in control subjects is associated with the likelihood of being resistant to breast cancer.}, Keywords = {Polymorphism, Breast Cancer, IL-17, Methylation, P16/Ink4}, volume = {8}, Number = {1}, pages = {628-636}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1405-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1405-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Soleimanpour, Elaheh and Babaei, Esmaeil and Hosseinpourfeizi, Mohammadali and Montazeri, Vahi}, title = {Evaluating long non-coding RNA PRNCR1 in breast cancer}, abstract ={Background & Objective: Breast cancer is one of the most common malignancies leading to death in women especially in industrial countries. Recent studies revealed that noncoding RNAs play important roles in various cellular activities such as tumor initiation, progression, and resistance to therapy. PRNCR1 is a long noncoding RNA that upregulates in some cancers and through androgen receptor signaling causes carcinogenesis. The aim of this study was to evaluate the expression pattern of PRNCR1 in breast cancer patients. Material & methods: In the present study, 30 breast tumor specimens and paired adjacent nontumoral tissues were collected from breast cancer women from East Azarbaijan province during the period of 2014-2015 and the expression level of PRNCR1 was evaluated using qRT-PCR. Also, the statistical analysis (t-test) was performed to examine the association between PRNCR1 and clinic-pathologic characteristics of tumor samples. Results: The data revealed that PRNCR1 significantly upregulates in breast tumor tissues compared to the paired adjacent normal tissues. Moreover, overexpression of PRNCR1 in breast tumor tissues was significantly related to tumor size and lymph node metastasis (P<0.05). Conclusion: The results revealed that PRNCR1 significantly dysregulates in breast cancer. Considering its effect on downstream pathways of androgen receptor, suggesting that it might be used as a therapeutic agent, although further studies are required.}, Keywords = {Breast cancer, long noncoding RNA, androgen receptor, PRNCR1}, volume = {8}, Number = {1}, pages = {637-646}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1439-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1439-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Sonbolestan, Elham and Sazgar, Hossein and Zia-Jahromi, Noosha and MohammadiFarsani, Farzaneh}, title = {Investigating the relationship between C677T polymorphism of the Methylenetetrahydrofolate Reductase Gene and the levels of homocysteine in primary and secondary infertile women in Isfahan}, abstract ={Background & Objectives: Methylene Tetrahydrofolate Reductase (MTHFR) gene, as an important genetic factor affecting infertility, encodes Methyl Dihydrofolate Reductase enzyme which helps to regulate the levels of homocysteine in the body. Mutations in this gene can reduce the activity of the enzyme, which leads to increase levels of blood homocysteine and may cause infertility. The purpose of this research was to investigate the relationship between C677T polymorphism of MTHFR gene and infertility. Material & Methods: In this study, 100 samples from infertile and fertile women with a history of abortion and 100 healthy pregnant were selected. After extracting the DNA from peripheral blood, the genotype of the samples was analyzed by PCR-RFLP method. SPSS software was also used to investigate the relationship between C677T polymorphism and infertility (using t. test, Chi-square test and logistic regression). Results: C677T polymorphism of MTHFR gene, resulted in a significant increase in the level of homocysteine and decrease levels of folate and vitamin B12 in homozygous women. However, there was not any relationship between the genotype of heterozygous patients with the level of homocysteine, folate and vitamin B12. Conclusion: C677T polymorphism of MTHFR gene may be associated with the increased levels of homocysteine and decreased levels of folic acid and vitamin B12 in the body, which can cause fetus recurrent miscarriage syndrome.  }, Keywords = {Methylene Tetrahydrofolate Reductase (MTHFR) gene, Infertility, Polymorphisms, Homocysteine, Restriction Fragment Length Polymorphism (RFLP)}, volume = {8}, Number = {1}, pages = {647-656}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1424-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1424-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Malekifard, Farin and Delirezh, Nowruz and Hobbnaghi, Rahim and Malekinejad, Has}, title = {Investigating the Effects of All-trans Retinoic Acid on Histopathology of Pancreas of streptozotocin -Induced Diabetes in C57BL/6 mice}, abstract ={Backgrounds & Objectives: Type 1 diabetes is an auto-immune disease and caused by insufficient insulin production by the body. All-trans Retinoic Acid (ATRA) is an antioxidant, anti-cancer and anti-inflammatory agent. The objective of the present study was to investigate the effects of ATRA on histopathology of pancreas in diabetic mice. Material & Methods: Diabetes was induced by multiple low-dose of streptozotocin injection (40 mg/kg/day for 5 consecutive days) in male C57BL/6 mice. After induction of diabetes, mice were treated with ATRA (20 mg/kg/day i.p.) for 21 days. On the last day, pancreases were isolated and stained with hematoxylin &eosin (H&E) and Gomeri aldehyde fuchsin (GAF) for histological analyses (the number of islets and β cells, diameter of islets) of pancreas. Results: ATRA treatment in streptozotocin-induced diabetic mice was increased the mean diameter of islets and the number of islets and beta cells compared to the diabetic group. (p<0/05). Conclusion: The administration of ATRA improved pancreas tissue during destruction of the pancreatic beta-cells in STZ-induced type 1 diabetes in mice.  }, Keywords = {Type 1 diabetes, All-trans Retinoic Acid, Pancrease, Streptozotocin}, volume = {8}, Number = {1}, pages = {657-665}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1430-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1430-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Fadaei, Sahar and ValipourChahardahcharic, Saeid and Sazgar, Hosei}, title = {Investigating the effect of intraperitoneal administration of zinc oxide nanoparticles on the model of postpartum depression in adult female mice}, abstract ={Background & Objective: Postpartum depression appears to be a harmful condition affecting mothers and their babies negatively. Regarding their side effects, the tendency to use antidepressants has especially fallen in nursing mothers. Considering the effect of zinc on mood, this study was conducted to determine the efficacy of zinc oxide nanoparticles on postpartum depression in female mice. Material & Methods: In this experimental study, the adult female mice of NMARI breed (25-30gr) were divided into six groups respectively (n=8): 1) Control 2) Depressed group 3, 4, 5) Depressed groups treated with a dose of zinc oxide nanoparticles including (5, 10 and 20 mg/kg) 6) Depressed groups treated for 8 days with a dose of 5 mg/kg zinc oxide nanoparticles. First, the studied animals were depressed by intraperitoneal injection of 5mg/kg progesterone for 5 days. The control group received no drug or solvent. The assessment of depression rate on the eighth day after the start of administration of progesterone was conducted by forced swim test. Results: The zinc oxide nanoparticles at doses 10 mg/kg (P< 0.01) and 20 mg/kg (P< 0.05) and also at 5 mg/kg dose for 8 days (P< 0.01) led to a significant decrease in immobilization time in depressed mice. Conclusion: Administration of progesterone induces depression, and thus, increases the period of immobilization of mice. The zinc oxide nanoparticles reduced the symptom of postpartum depression in forced swim test. The findings can be used to show the effects of zinc oxide nanoparticles in the reduction of postpartum depression.  }, Keywords = {Postpartum depression, Zinc oxide nanoparticles, Progesterone, Mice}, volume = {8}, Number = {1}, pages = {666-673}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1463-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1463-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Maljaee, Pariya and Zolfagharian, Hossein and Babaie, Mahdi and MohammadpourDounighi, Naser}, title = {The comparison of nanoparticle adjuvant with the montanide adjuvant for hyper-immunization to produce anti-snakebite serum}, abstract ={Background & Objective: Currently selection and use of nanoparticle adjuvant is very important. Due to the lack of effective anti-snakebite serums to treat it and high cost of purchasing external anti-snakebite serums, preparation of suitable internal nanoparticles to produce hyper-immunization plasma has become very important. In order to minimize the disadvantages associated with traditionally used adjuvants in ovines and to produce potent antivenum, a comparison was made between various adjuvants for their immune-potentiation capacity and safety. Material & methods: The present study was conducted in 15 sheep, divided into three groups and hyper-immunized using crude venom of cobra snake (Naja naja oxiana) along with three adjuvants, Montanide, aluminum phosphate and poly methyl methacrylate through subcutaneous route at intervals of a week. Periodic standard safety assessments were done. Results: The neutralization activity (LD50) of pooled sera samples by 9th week, obtained with aluminum phosphate, Montanide and poly methyl methacrylate groups were 1.5, 2.2 and 2 LD50/ml respectively. The sheep of poly methyl methacrylate group showed minimum local reactions at injection site, while sheep from other two groups exhibited moderate reactions. However, these were transient and reabsorbed or healed subsequently. Conclusion: Poly methyl methacrylate nanoparticle adjuvant could be a possible alternative to the emulsion adjuvants for primary phase of immunization in antivenomous preparation considering its acceptable immunopotentiation capacity and safety in donor animals.  }, Keywords = {Nanoparticle adjuvant, Naja naja oxiana, Poly methyl methacrylate, aluminum phosphate, Montanide}, volume = {8}, Number = {1}, pages = {674-682}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1470-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1470-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Shaki, Maasomeh and Baay, Naser and Mazraeh, Ahm}, title = {Investigating The Effect of progressive Resistance Training on serum homocysteine levels, Insulin resistance index and lipid risk factors in ovariectomized rat}, abstract ={Background & Objectives: Menopause is associated with increased cardiovascular risk factors. This investigation examined the effects of resistance training on cardiovascular risk factors in ovariectomized rats. Material & methods: In this experimental study, 16 adult ovariectomized female Wistar rats were randomly allocated into control (n=8) and resistance training (n=8) groups. The progressive resistance training protocol was administered for 10 weeks (5 sessions per weeks). Training protocol consisted of 8 series of climbs on the ladder with progressive overload attached to the tail and each series contained an average of 8 to 12 climbing movements (repetitions). Forty eight hours after the last training session, animals were anesthetized, blood was taken directly from the heart. Then serum homocysteine levels and lipid profile were measured and insulin resistance index was calculated. All variables were compared by unpaired t-test) p< 0.05(. Results: serum homocysteine levels (p = 0.013) and insulin resistance index (p = 0.019) were significantly decreased in resistance training group compared with the control group, but lipid profile did not differ among rat groups (p˃0.05).  Conclusion: It seems that ten weeks of progressive resistance training reduced serum homocysteine levels and insulin resistance index in ovariectomized rats, but did not effect on the serum lipid profile.    }, Keywords = {progressive resistance training, homocysteine, Insulin resistance index, ovariectomized rat}, volume = {8}, Number = {1}, pages = {683-691}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1487-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1487-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Soleymanian, Marzeyeh and Tajbakhsh, Elahe and Bamzadeh, Zahr}, title = {Investigating Antibiotic Resistance Pattern and Prevalence of Class I, II, III Integron Genes in K. Pneumoniae Isolated from Clinical Samples in Sahrekord, Iran}, abstract ={Background & Objective: Acquiring integron is an important factor in multidrug resistance in the intestinal gram negative microorganisms. The most common integron cassette contains the genes associated with resistance to a wide range of antimicrobial agents. The aim of this study was to determine the prevalence of class 1, 2 and 3 integron genes in Klebsiella pneumoniae strains which was isolated from clinical samples in Shahrekord. Material & Methods: In this study antibiotic resistance pattern of 64 strains of Klebsiella pneumonia isolated from clinical samples in medical diagnostic laboratories in Shahrekord were tested by disk diffusion. In order to determine the prevalence of integrons class 1, 2 and 3 were used as specific primers. Results: After performing antibiogram tests, the most resistant was observed to be ampicillin (90.6%) and the lowest resistance one was imipenem (9.3%). Class 1, 2 and 3 integron were observed in 8 isolates (12/5%), 6 isolates (9/4%) and 10 isolates (15/62%). In 40 isolates were not observed Integron genes. In the statistical analysis by Fisher exact test between class 1 integron and resistance to the ampicillin significant association was observed. Conclusion: Resistance genes are located on the integrons and can be transmitted from one strain to another and disseminate resistance in the hospital or other environments, it is important to identify these types of antibiotic resistance genes twofold has done.}, Keywords = {Antibiotic resistance, Integron, Klebsiella pneumonia}, volume = {8}, Number = {1}, pages = {692-700}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1148-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1148-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Moradi, Zahra and Moradi, Parvin and Sharafi, Mehdi and Akbarzadeh, Zahr}, title = {Evaluation of wound complications after cesarean section and its related factors in women referring to Vali-e-Asr hospital FASA 1394}, abstract ={Background & Objectives: Endometritis is the most common complication of cesarean section and can cause complications such as prolonged hospitalization, additional costs, establish long-term use of antibiotics, adhesions and infertility. The prevalence of cesarean section infection has been estimated 3 to 15 percent. The incidence of infection depends on socio-economic factors, length of labor, parity, the duration of the rupture of membranes (ROM) or amniorrhexis, and the mode of delivery. The perioperative application of antimicrobial drugs has changed it radically. Material & Methods: This is a cross-sectional study that required information was collected from 300 women who had cesarean in Vali-e-Asr hospital. A questionnaire was filled out at mothers’ bed, in women's ward. Data were analyzed by chi square test through SPSS software. (P<0.05) Results: Among the 300 cases, 15 patients (5%) had postpartum infection. There was a significant difference between post section infection and cesarean section (elective or emergency), maternal education level, maternal age and parity, prenatal care, type of anesthesia. There was no significant relationship between infection after cesarean section and average age of the women, PROM, gestational diabetes, BMI. Conclusion: Education level, parity and prenatal care, type of cesarean section, type of anesthesia, are the risk factors of post cesarean infection. We hope to control the incidence of preventable dangerous complications of post section infection and reduce health consequences for the mother as well as the health system.  }, Keywords = {pregnancy, cesarean section, Surgical Wound Infection}, volume = {8}, Number = {1}, pages = {701-707}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1294-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1294-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Aminshahidi, Maneli and Firoozian, Nasrin and Rafaatpour, Nouredin and Fani, Fereshteh}, title = {Investigating vegetables from different areas of Shiraz and water used for their irrigation in terms of contamination with bacteria causing foodborne and waterborne diseases in summer 95}, abstract ={Background & objectives: since consumption of raw vegetables is part of our daily regimen, their microbial contamination is important for gastrointestinal diseases. The aim of this study is to evaluate not only the contamination of vegetables in different parts of Shiraz, but also the water used to irrigate those same regions in terms of enteropathogenic bacteria. Materials & Methods: three types of raw vegetables – leeks, purslane, and basil – as well as water samples used for the irrigation of those vegetables were collected from the vegetable farms of nine different regions in Shiraz. Bacterial culture and biochemical tests were used for the identification of Coliforms and enteropathogens. The identification of different pathotypes of Escherichia Coli (E. coli) was done using molecular tests. Antibiotic susceptibility testing was performed by disc diffusion method. Results: in these 9 sampling farms, 60, 50, 50, 28.5, 33.5, 33.5, 60, 40, and 16.5 per cent of isolated bacteria in genus level were similar between the vegetables from the 9 sampling farms, and the water samples of the same regions. We isolated two enter hemorrhagic E. coli from purslane and one Aeromonas hydrophila from the water sample. All three isolates were susceptible to broad spectrum beta-lactams. Conclusions: The microbial contamination of the water used to irrigate the raw vegetables has affected their health and this could cause gastrointestinal diseases. This indicates that the quality control of these resources in terms of microbial contamination is valuable not only for maintaining public health, but also for preventing gastrointestinal outbreaks.    }, Keywords = {Vegetables, Escherichia Coli, Water, Bacterial contamination}, volume = {8}, Number = {1}, pages = {708-717}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1565-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1565-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Musavi, Negar and Tafvizi, Farzaneh and Mansoori, Yaser}, title = {The association between polymorphism XRCC1 (rs25487) and the susceptibility of chemical industry workers to benzene}, abstract ={Background & Objective: Benzene, as a carcinogenic compound, can damage DNA by producing free oxygen radicals. Benzene effects have been reported in the blood system. It seems that XRCC1 gene, as a gene involved in the repair of damaged bases, plays a role in the sensitivity of individuals to benzene. The purpose of this study was to investigate the association between rs25487 polymorphism in XRCC1 gene and the susceptibility of chemical industry workers against benzene. Material & Methods: In this case-control study, 60 cases and 60 controls who were exposed to benzene for 2 consecutive years were examined. People who did not have any changes in blood parameters were selected as the control group and those who have shown lymphocytes outside the normal range were considered as a case group. Blood samples were collected from chemical workers. Gene polymorphism was determined by RFLP-PCR using MSP1enzym. Results: There was no significant difference between allelic frequencies A and G (P >0.05). No significant association was found between XRCC1 polymorphism and benzene susceptibility and lymphocytic abnormalities (OR: 1.43, 95% CI (0.47 - 4.31), P = 0.52). Conclusion: It seems that rs25487 polymorphism in the XRCC1 gene does not play a role in the sensitivity of individuals to benzene. Of course, due to the role of XRCC1 gene in response to DNA damage, other polymorphisms of this gene and polymorphism that are targeted in this study are evaluated at a wider level.}, Keywords = {Polymorphism, XRCC1, Benzene, RFLP-PCR}, volume = {8}, Number = {2}, pages = {718-726}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1621-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1621-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Shokrollahi, Shekoofeh and Heshmati, GholamAli and YosefZadeh, Hame}, title = {Study the Phenolic Compounds and Antioxidant Activity of the Extract Lily (Lilium ledebourii (Baker) Boiss)}, abstract ={Background & Objective: Lilium ledebourii (Baker) Boiss, is the only national and natural heritage of Iran, which grows in mountainous regions of Hyrcanian forests with limited distribution that many ecological, ornamental and medicinal aspects of this plant has not been studied yet. Material & Methods: Ethanolic extract was prepared by maceration method. Then, total phenol and total flavonoids of ethanolic extracts were respectively performed by the Folin- Ciocalteu and AlCl3 method. Antioxidant activity was measured by scavenging free radicals of DPPH. Statistical analysis of the data was performed by ANOVA (Analysis of Variance) and Duncan Test to compare the means. Results: The total phenols of Lily shoots was between 59/6 ± 65/43 and 21/9 ± 14/66 and its antioxidant activity was between 51 to 90 percent at four habitats, The highest and lowest amount of total phenols and antioxidant activity of Lily shoots obtained from Kelardasht (maximum altitude) and Rostam Abad (minimum altitude). The total flavonoid were reported between 91/7 ± 41/33 mg GAE g-1 and 21/1 ± 72/43 mg QUE g-1. The highest and lowest content of Total flavonoid were respectively observed in Asalem and Namin. Conclusion: The results showed that the antioxidant capacity of Lily is relatively high compared to other medicinal plants, also antioxidant activity of the Lily extract is variable in different sites and significantly affected by habitat altitude.}, Keywords = {Lily, antioxidant activity, flavonoid, extraction, habitat}, volume = {8}, Number = {2}, pages = {727-734}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1363-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1363-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Forouzesh, Flora and Talebi, Saei}, title = {Anticancer effects of sodium butyrate on human colon cancer Caco2 cell line}, abstract ={Background & Objective: Sodium butyrate (NaBu), a histone deacetylase inhibitor, is an anticancer drug for multiple cancers. The objective of this research is to study the anticancer effects of sodium butyrate on colon cancer Caco2 cell lines. Material & Methods: Caco-2 cell lines were cultured and then treated with various concentrations of NaBu (from 3.125mM to 200mM). After 24h, 48h and 72h incubation, the cytotoxicity was measured by XTT assay. The statistical significance of difference analyzed by one-way ANOVA/Tukey test. Also, in this research, the morphologic changes are considered by invert microscopy. Results: The results showed that NaBu inhibited the growth in a time- and dose-dependent manner. XTT assays showed that treatment with NaBu at high concen­trations significantly inhibited the growth of Caco2 cells. After 24, 48 and 72h, IC50 for Caco2 cells were 105, 100 and 43 mM respectively. Results confirmed the anti-proliferative effect of Sodium butyrate on this cell lines. The results of morphological studies indicated that sodium butyrate has a growth inhibitory effect on IC50 concentration and it can arrest the cell cycle. Conclusion: These results demonstrate that butyrate inhibited the growth of colorectal cancer cells. It caused probably cell death via the induction of apoptosis. These findings may open interesting perspectives in human colorectal cancer treatment strategy.  }, Keywords = {colorectal cancer, Apoptosis, sodium butyrate, histone deacetylase inhibitor, Caco2 cell line}, volume = {8}, Number = {2}, pages = {735-745}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1501-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1501-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Mirzaei, Nasim and Kaka, Gholamreza and Azarnia, Mahnaz}, title = {The effects of Elaeagnus Angustifolia fruit aqueous extract on histomorphometric changes of hippocampus in Balb/c mouse embryo}, abstract ={Background & Objective: The use of herbal medicine rapidly increased because in comparison with chemical drugs they have less harmful health effects. Since some pregnant women due to have joint and muscle pains may be taken administration of Elaeagnus angustifolia by their own or physicians and the use of Elaeagnus angustifolia may have adverse effects on their fetuses development. This study investigated the effect of aqueous extract of Elaeagnus angustifolia on histomorphometric changes of the hippocampus of mouse fetuses. Material & Methods: : Twenty-one pregnant mice were randomly divided into three groups. The control group received sufficient amount of drinking water, the sham group received 20 ml of water (solvent of Elaeagnus angustifolia) daily and the experimental group received aqueous Elaeagnus angustifolia extract at dose of 500 mg / kg daily from 0 to 20  days of gestation. Pregnant mice were killed on the 20th day of pregnancy and the fetuses were removed and examined for external congenital malfomations. Fetal body weights and crown-to-rump lengths were measured. The head of fetuses were fixed and processed  and were then embedded and their heads were coronally sectioned and were then stained with hematoxylin-eosin. Finally the hippocampus of fetal brain were examined using a light microscope and the Motic software. Finally, the thickness and cell number of radial, stratum  oriens, molecular and ventricular hippocampus were evaluated using ligth microscopy and Motic software. Results: Mean fetal weight and CRL of fetuses and mean thickness of ventricular layer in CA1 and CA2 significantly increased in experimental group compared to control and sham groups. The mean thickness of radiatum layer of CA3 significantly decreased in experimental group compared to control and sham groups. Additionally, mean number of cells in ventricular layer of CA1 significantly increased in experimental group compared to control and sham groups. Conclusion: Administration of aqueous extract of Elaeagnus angustifolia to pregnant mice can cause changes in fetal development and cause some changes in the hippocampal formation CA1 and CA3 neuronal layer in the mouse embryo.  }, Keywords = {Fetus, Elaeagnus angustifolia, Hippocampus, Mice}, volume = {8}, Number = {2}, pages = {746-753}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1451-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1451-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Tahmasebifar, Zahr}, title = {Investigating the relationship between Body Mass Index (BMI) and C11377G polymorphism of adiponectin gene with breast cancer}, abstract ={Background & Objective: Adiponectin hormone is thought to be a component of obesity hormones and is a contributing factor in breast cancer, which increases the genetic variation of this effect. The aim of this study was to investigate the relationship between C11377G polymorphism of adiponectin gene and BMI with the risk of breast cancer. Material & Methods: For this study, 128 patients with breast cancer and 128 healthy subjects were blood sampled. After the extraction of DNA by salting out method, the specimens were amplified with specific primers. After the PCR products were evaluated, some parts were utilized for determining the genotype of individuals by using the PCR-RFLP technique. At the end of the study, the results were analyzed by SPSS-23 software and Chi-square statistical analysis. Results: The G allele frequency was 0.28 in the cancer group and 0.27 in the control group. There was no statistically significant association between the two groups for this polymorphism. By studying the relationship between this gene variation and other variables, it was found that C11377G polymorphism had a significant correlation with body mass index (p value=0.038, OR: 1.766, CI95%: 1.030-3.028) and smoking (p value=0.029. OR: 1.979, CI95%: 0.893-4.388). Conclusion: The C11377G polymorphism of Adiponectin did not show a significant relationship and risk of breast cancer. However, body mass index showed a significant relationship with breast cancer. Also, people who carry this genetic change and high body mass index or smokers were prone to breast cancer.      }, Keywords = {Breast Cancer, C11377G Polymorphism, Adiponectin, Body Mass Index, RFLP-PCR}, volume = {8}, Number = {2}, pages = {754-759}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1485-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1485-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Bigdeli, Reihane and Rezaie, Omi}, title = {Comparison between anticipatory effect of Alexithymia and Emotion regulation Difficulties (disorder) on language impairment in Schizophrenia patients.}, abstract ={Background & Objective: Schizophrenia is one of the most fundamental challenges related to mental health. Linguistic disorganization and dysregulation are considered the main symptoms of schizophrenia diagnosis. The aim of the present study is to evaluate the anticipatory effect of alexithymia and emotional-dysregulation disorder on language disorders in schizophrenic patients. Material & Methods: This is a descriptive and analytic study in cross-sectional correlation method. Sample groups are from 81 patients who were selected using purposive sampling method. They are all psychotic patients that were hospitalized at the Razi psychiatric hospital of Tehran, and they are educated as high as high-school diploma in 2016. Participants completed the following questionnaires: Toronto Alexithymia-Scale (TAS-20), the Difficulties in Emotion Regulation-Scale (DERS) and the Farsi Aphasia-Test (Nilipoor). The collected data were analyzed using inferential statistics of regression analyzing data, multivariate analysis of variance and Pearson correlation coefficient through SPSS-22. Results: Results showed that the variable of alexithymia had a stronger anticipatory role in language impairments than the variable of emotional regulation among schizophrenic patients (P<0/01) was observed between these two variables and language impairments. The Results of regression analysis of these two on the subscales of language impairments showed that these variables (respectively) had the highest impact on improvised-conversation and listening-comprehension and the lowest impact on oral-expression. Also it could be concluded that about 19% of language impairments’ variance could be predicted by two variables of alexithymia and emotional dysregulation. Conclusion: Results of this study showed that alexithymia and emotional dysregulation disorder could be important psychological factors for predicting schizophrenia.  }, Keywords = {Emotional Dysregulation Disorder, Language impairment, Schizophrenia, Alexithymia}, volume = {8}, Number = {2}, pages = {760-769}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1505-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1505-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Nejat, Nahid and Aleyasin, Seyed Ahmad and Angaji, Seyed Abdolhamid and Ayerian, Saie}, title = {Investigating Methylation changes of Vimentin gene in Breast Cancer}, abstract ={Background & Objective: Epigenetic changes are one of the most common changes in development of cancers. These changes alter the expression of certain genes that play important roles in the etiology of cancer. Increasing the expression of Vimentin gene due to epigenetic changes in Epithelial-Mesenchymal Transition(EMT) in breast carcinoma is well known. The relation between EMT and the malignancy invasion process has also been confirmed. Vimentin is the principal EMT marker that performs this task during metastasis. The purpose of this study is to investigate the methylation of the vitamin gene in a specific position. Material & methods: In this case-control study, the methylation pattern of the promoter of the Vimentin gene was evaluated in a specific site. Blood samples were taken from 30 breast cancer patients and 30 healthy individuals. DNA samples were digested with the methylation sensitive restriction enzyme. Control and treated DNA was amplified by PCR for qualitative investigation and real time PCR for quantitative analysis of methylation. Results: Using the Ct value and the%Me=100(e-0.7(ΔCt)) formula, the correlation between Vimentin hypomethylation and expression of Estrogen Receptor(ER), Progesterone Receptor(PR) and Human Epidermal growth factor Receptor2(HER2) status was checked (p-value <0.05). Conclusion: The results indicate that hypomethylated promoter of the Vim gene in the examined site correlates with ER, but does not have any correlation with PR and Her2, and hence the hypomethylation of the Vimentin gene in this position can be proposed as a molecular biomarker}, Keywords = {Breast cancer, Methylation, Vimentin, Restriction Enzyme PCR, Real Time PCR}, volume = {8}, Number = {2}, pages = {770-777}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1507-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1507-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Jamshidi, Hasan and Baziarsarani, Saman and Khoramisarvestani, Soheil and Saadat, Pouria and Sabet, Hamid rez}, title = {The Assessment of Changes in Cesarean Indications Before and After the Implementation of Health Sector Evolution Plan in Valiasr Hospital, Fasa, Fars Province in 2013-2015}, abstract ={Background & Objective: Considering the importance of cesarean section and its effect on maternal and neonatal health, we studied the effect of health system on cesarean section indications before and after the Implementation of Health Sector Evolution plan in Hazrat-e-Valiasr Hospital of Fasa. Material & Methods: The present study is a descriptive cross-sectional study of retrospective type. Cesarean indications are based on the checklists of the treatment vice-chancellor which includes: previous cesarean sections, fetal abnormal presentation, fetal distress, cephalopelvic disproportion, failure to progress, twin pregnancy, postdate pregnancy, placenta previa, placenta abruption and other causes. Sample volume comprised all pregnant women referring to Hazrat-e-Valiasr Hospital of Fasa. This study investigated cases of pregnant mothers within one year before and one year after the Implementation of Health Sector Evolution Plan (May 5, 2014). Results: According to the findings of this study, the Implementation of Health Sector Evolution Plan caused a significant reduction in the rate of cesarean section (p value = 0.024). Some of indications such as previous cesarean section, Failure to progress and Placenta Abruption increased (p <0.05). And there was a decrease in some cases such as: Postdate pregnancy and other causes (p <0.05). There was no significant alternation in other indications. Conclusion: Based on the findings of this study, it can be stated that the Implementation of Health Sector Evolution plan caused a comparative reduction in the rate of cesarean section and an increase in normal delivery.  }, Keywords = {Health Sector Evolution Plan, Vaginal Delivery, Cesarean, Indication}, volume = {8}, Number = {2}, pages = {778-784}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1523-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1523-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Mousavi, Golchin and Hasanzadeh, Shapour and Malekinejad, Hasan and Najafi, Gholamrez}, title = {Protective effects of Spirulina (Arthrospira Platensis) on In Vitro Fertilization (IVF) and embryo development in female mice treated with cyclophosphamide}, abstract ={Background & objective: Cyclophosphamide is an extensively used chemotherapeutic agent against wide varieties of neoplastic ailments, which has been known to cause ovarian damages and infertility in mammals. The purpose of this study is to investigate the protective effects of Spirulina platensis on in vitro fertilization (IVF) and embryo development in adult mice exposed to Cyclophosphamide. Material & methods: 40 adult female mice were divided into 8 groups each comprised 5 animals. The first group was considered as control. The groups 2, 3 and 4 were exposed to Spirulina at rates of 200, 400 and 800 mg/kg respectively through oral route, daily for 28 days. The 5th group receive cyclophosphamide (150 mg/kg, ip) in a single-dose. Groups 6, 7 and 8 received cyclophosphamide and Spirulina together with aforementioned doses. In vitro fertilization and embryo development were assessed in all groups at the end of experiment. Results: The groups which received Spirulina at rates of 200, 400 mg/kg beside cyclophosphamide in comparison to group which received merely cyclophosphamide, the number of oocytes, percentages of fertilization, two-cell embryos, blastocysts and hatched embryos were reduced significantly (p<0.05). In fertilization, two-cell embryo, but at dose of 800 mg/kg was not able to reduce damages brought by cyclophosphamide. Conclusion: According to the results of this study, spirulina encourages protective effects on cyclophosphamide induced reprotoxicity on In Vitro Fertilization approach.  }, Keywords = {Spirulina, cyclophosphamide, in vitro fertilization, mouse}, volume = {8}, Number = {2}, pages = {785-794}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1511-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1511-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Karami, Azam and Khaksar, Zabihollah}, title = {The effect of Panax ginseng extract on histomorphometric changes on brachial plexus region of the spinal cord in 20 days fetuses of diabetic rats}, abstract ={Background & Objective: Diabetes mellitus is a group of metabolic disorders characterized by increased levels of glucose. Ginseng has been used to control the diabetes for many years ago. This study investigated the effect of Panax ginseng extract on histomorphometric changes on brachial plexus region of spinal cord in 20 days fetuses of diabetic rats. Material & Methods: 16 rats were divided into four groups: non-diabetic control, non-diabetic recipient of the extract, diabetic control and diabetic recipient of the extract. Diabetes was induced by streptozotocin in diabetic groups and all groups became pregnant. During pregnancy, recipient of the extract groups received ginseng extract every day. On the 20th day of pregnancy, the mothers were anesthetized, and the fetuses were obtained. Brachial region of the spinal cord with vertebral column separated after using the techniques of histology, some histological factors were measured. Data were analyzed by using SPSS software and ANOVA and Duncan statistical tests (P≤0.05). Results: A significant difference was observed in the transverse and vertical diameter of central canal of the spinal cord in brachial plexus region of fetuses in diabetic control group than non-diabetic groups (P≤0.05). A significant reduction was observed in height of ependymal cells and cell count of gray matter of spinal cord in diabetic control group than other groups (P≤0.05). Conclusion: Extract of Panax ginseng can control hyperglycemia and manage diabetes on spinal cord in fetuses of mothers with gestational diabetes by increasing β-cells stimulation and increasing insulin production.  }, Keywords = {Diabetes mellitus, brachial plexus region of spinal cord, Panax ginseng extract, Streptozotocin, Rat}, volume = {8}, Number = {2}, pages = {795-804}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1513-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1513-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Abdollahi, Solmaz and Pourahmad, Afshin and Asadpour, Leil}, title = {Synthesis and characterization of Graphene - ZnO NPs nanocomposite and its application for antibacterial activities}, abstract ={Background & Objective: The investigation of the antibacterial activity of nanomaterials has regained importance due to increasing bacterial resistance to antibiotics. Inorganic antibacterial materials have several advantages over traditionally used organic agents; like chemical stability, thermal resistance, safety to the user, long lasting action period, etc. In this research, antibacterial properties of graphene - zinc oxide nanoparticles nanocomposite was evaluated for gram-positive (S. aureus) and gram-negative (E. coli and Pseudomonas aeruginosa) bacteria. Material & Methods: The current study demonstrates homogenous decorating of zinc oxide nanoparticles (NPs) onto graphene oxide (GO) surface via simple chemical method. Disk diffusion method and MIC were used to evaluate the antibacterial activity of ZnO nanoparticles on S. aureus, E. coli and Pseudomonas aeruginosa compared to standard commercial antibiotic disks. Results: XRD results revealed diffraction peaks for each of the two compounds in the nanocomposite. The XRD studies showed that the synthesized ZnO NPs have hexagonal wurtzite structure. It was found that GO addition induces a decrease in crystallite size. Average size of the ZnO NPs was estimated by transmission electron microscopy around 20 nm. ZnO nanoparticles showed bactericidal effects on Gram-positive and Gram-negative bacteria. The improved antibacterial activity of ZnO nanoparticles compared to its microparticles was related to the surface area enhancement in the nanoparticles. Conclusions: The novel nanocomposite exhibits excellent antibacterial activity against gram-positive (S. aureus) and gram-negative (E. coli and Pseudomonas aeruginosa) bacteria.      }, Keywords = {Graphene oxide, ZnO nanoparticles, Nanocomposite, Antibacterial activity}, volume = {8}, Number = {2}, pages = {805-814}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1524-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1524-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Yadegari, Mehdi and Riahy, Simin and Mirdar, Shadmehr and Hamidiyan, Gholamreza and Mosadegh, Parinaz and Yahyavi, Farib}, title = {Evaluating the effects of hypoxia and taper on expression of Pulmonary Bax and Bcl2 apoptotic proteins}, abstract ={Background & Objective: Due to The limitation of research literature, related to the effect of exercise training with different intensity, Along with Hypoxia Stress on Pulmonary Apoptosis, The purpose of this study was to investigate the effects of hypoxia and taper on expression of Bax and Bcl2 apoptotic proteins of lung.   Materials & Methods: In this experimental study Twenty-four rats (4-week old, 72±8 gr) were divided accidentally to control and training groups. The samples after 6 weeks anaerobic exercise training, were exposed to Environment hypoxia and lived three weeks over there. Another group Concurrent with exposure to hypoxic environment, implementation Taper (30% reduction in exercise intensity) technique. In lung tissue, the expression of Bax and Bcl2 was studied by the immunohistochemical methods. One-way Anova was performed to analyze data. P≤0.05 was considered statistically significant. Results:Expression of Bcl2 protein in trained rats and exposed to hypoxic stress was increased And reduced after a 3-week use of the taper(P>0.05). Expression of Bax protein in trained rats and exposed to hypoxic stress was increased (P≤0.05), and reduced after a 3-week use of the taper (P>0.05).   Conclusion: It seems that, Medium hypoxia is able to apply Apoptotic effects in lung of rats that underwent intensive exercise and likely the use of intensity taper reduces these effects.    }, Keywords = {Apoptosis, Bax, Bcl2, Hypoxia, Taper}, volume = {8}, Number = {2}, pages = {815-824}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1526-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1526-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Bamdad, Kourosh and Khorramnejad, Raziyeh}, title = {Assessing the consequences of treatment with 131I on calcium, vitamin D3, parathormone, and cortisol in patients with thyroid abnormalities}, abstract ={Background & Objective: Consequences of radioisotope Iodine-131 therapy on tissues, organs, and blood parameters is a major concern in patients suffering from thyroid abnormalities that were treated with this classical treatment. Thus the aim of the present study is to assess the variations in the content of calcium, vitamin D3, parathyroid hormone, and cortisol in patients treated with radioiodine. Material & Methods: In two different stages, the first step before and then 48 hours after oral administration with radioactive iodine, venous blood from 132 cancerous thyroid female patients was analyzed. There was at least a two-month time gap between their thyroid surgery and the initiation of radioiodine therapy and it was the first time that patients were admitted for treatment with radioactive iodine. In this 48-hour period variations in calcium, vitamin D3, parathyroid hormone, and cortisol of the blood serum was measured. Each patient was compared to herself; however, the comparison of the entire community was also taken into considerations using statistical methods. Results: The results showed a significant decrease in levels of vitamin D3; after treatment with Iodine-131. Parathyroid hormone was also significantly reduced after 131I uptake; however, calcium levels remained almost unchanged. On the other hand, the cortisol level increased significantly. Conclusion: Increasing of cortisol levels; lowering the content of vitamin D3, and decreasing the amount of parathyroid hormone are consequences of treatment with 150 mCi Idine-131 in the understudied population.}, Keywords = {Calcium, Vitamin D3, Parathyroid hormone, Cortisol, Iodine-13}, volume = {8}, Number = {2}, pages = {825-833}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1489-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1489-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Mostaghimi, Arezo and TorabzadehKhorasani, Parvin and Ramezani, Mi}, title = {The effect of retinoic acid on uterine tissue and estradiol, progesterone hormones in female adult BALB/c mice}, abstract ={Background & Objectives: Retinoic acid is recognized as a regulator of the growth and cell differentiation and is necessary for normal reproductive in male and female. The present study was done to investigate the effect of retinoic acid on the uterine tissue and estradiol and progesterone hormones in female adult BALB/c mice. Material & Methods: In this study after determining the appropriate dose of retinoic acid, it was injected intraperitoneally (IP) for 30 days On 75 adult BALB/c mice with doses of 15 (Group I), 25 (Group II), and 35mg/kg.b.w (Group III), control group (non- injection) and sham (distilled water injection). ANOVA analysis and Duncan test were applied to compare the means with significance level (P˂0.05) and (P˂0.001). Results: Uterine tissue studies indicated an increase in overall thickness of the uterus (endometrium, myometrium, perimeterum) (P<0.001), (P<0.05) increase number of closed glands, and decrease in open glands (P<0.001). The level of estradiol increased (P<0.05) and progesterone secretion decreased (P<0.05). Conclusion: It can be concluded that retinoic acid has damaging effect on the uterus tissue and sexual hormones, and its use especially in female should be by awareness and under physician supervision.}, Keywords = {Retinoic acid, Uterus, Estradiol, Progesterone, Mice}, volume = {8}, Number = {2}, pages = {834-840}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1492-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1492-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {}, title = {A review on the most valuable biomarkers in epilepsy}, abstract ={Background & Objective: Epilepsy is a common neurological disorder with the prevalence of 50 million in the world. Despite of many researches and introducing of different anti-epileptic drugs, 30% of patients suffer from refractory seizures. Biomarkers are the measurable indicators to detect the physiological and pathological condition. Biomarkers in epilepsy can be utilized for prediction, diagnosis, severity and effectiveness of drugs. Conclusion: The efficacy of biomarkers in the prognosis and evaluation of drugs effectiveness is notable. Owing to the diversity of clinical symptoms of epilepsy, various biomarkers have been presented. Among wide-ranging biomarkers, some structural biomarkers such as imaging and electrophysiology including electroencephalogram are the most usable biomarkers. These evidences suggest more investigations to introduce biomarkers that are more practical.}, Keywords = {Epilepsy, Biomarkers, brain}, volume = {8}, Number = {3}, pages = {841-853}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1510-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1510-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {karimi, elham and sadri, minoo and hosseini, motahare and heidari, Ali and kargar-ghalesefi, Samane and Krimimoghadam, Zeinab}, title = {Synthesis of Zataria Multiflora extraction loaded chitosan/PEO nanofibers via electrospinning method for potential biomedical applications}, abstract ={Background & Objective: The nanofibers are referred to as fibers with a diameter of less than 1000 nm. There are various ways to make nanofibers, one of which is electrospinning. In this method, nanofibers are formed on aluminum sheets. The conversion of chitosan to derivatives with greater solubility, its mixing with other polymers and the use of special solvents are methods for its ease of use. In this study, antimicrobial thyme extracts were prepared for the synthesis of chitosan / polyethylenoxide and thyme nanofibers by electroporation. Materials & methods: Using electrospinning, chitosan and polyethylenoxide were prepared Ratio of 90 to 10 and 80% tween in a 0.5 molar acetic acid solution. Thyme extract was added and after the synthesis of nanofibers, images of SEM were taken, then the diameter of the nanofibers with clemex vision software was measured. Results: According to the scanning electron microscopy (SEM) images and the nanofibers diameter obtained from the solution with chitosan / polyethylenoxide / thyme formulation with Ratio of 90 to 10, the mean diameter of nanofibers was in the range of 100-60 nm (best fiber with the lowest node). Conclusion: Prepared nanofibers are widely used in the medical industry, especially in the field of tissue engineering and wound scaffolds and also are used as antibacterial candidate. In addition to being effective in wound healing, they also reduce the cost of treatment.}, Keywords = {Chitosan, Polyethylenoxide, Nanofiber, Electrospinning, SEM}, volume = {8}, Number = {3}, pages = {854-861}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1618-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1618-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Modaresi, Mehrdad and Amoorizi, Masoume}, title = {Effect of Hydro-alcoholic Extract of Rosemary in Comparison with Alprazolam on Learning under Stress Conditions in Rats}, abstract ={Background & Objective: Many anti stress drugs have a lot of side effects on learning. The aim of this study was to compare the effect of Rosemary plant and Alprazolam on learning under stress conditions in laboratory mice. Materials & Methods: In this experimental study, 60 adult female rats were used. In order to investigate the learning capacity under stress conditions, the rats were divided into 6 groups of control, stress, alprazolam and the three experimental groups that received the hydro-alcoholic extract of rosemary plants at doses of 50, 100 and 200. In all of the samples (except the control group) 30 minutes after injection, the mice were placed in a dark box for 50 minutes for stress. Subsequently, observational behaviors of the rats were recorded by using mouse maze. The results were analyzed using SPSS software. Results: The results showed that rosemary hydro alcoholic extract at 100 and 200 mg/kg compared to the stress and alprazolam group significantly decreased the time to reach the target box in the Maze form, which indicates a reduction on effect of stress on the learning, while the number of errors in selecting the target box (reaching the target) at a dose of 200 mg/kg shows a significant decrease with the stress and drug group. Conclusion: According to the results, effective compounds in rosemary extract in two doses of 100 and 200 mg/kg can increase the learning in these conditions with the effect of reducing stress.    }, Keywords = {Rosemary, Learning, Stress, Alprazolam, T-shaped maze, Mice}, volume = {8}, Number = {3}, pages = {862-869}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1475-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1475-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Ghasemi, Fatemeh and Zaravar, Foroozandeh and Badiee, Paris}, title = {Determining of Fungal Contamination and Survey the Respective Effective Factors in Public Indoor Swimming Pools, Shiraz, Southern Iran}, abstract ={Background & Objective: Public swimming pools are susceptible places for the spread and transmission of pathogenic fungi. This study aims to determine fungal contamination in the environment and water of swimming pools and its relationship with the water chlorine level, temperature, pH and turbidity, to promote the public health system. Materials & Methods: Water samples were collected from 13 indoor public swimming pools in Shiraz and filtered. Temperature, chlorine level, pH and turbidity of the pools were measured on site. Samples from different parts of the pools were collected using 4×4 sterile carpet and cultured on Sabouraud dextrose agar. Fungal identifications were performed by the macroscopic and microscopic characteristics, and complementary tests. The identification of dermatophyte species was done by PCR-RFLP method. Results: The average pH, chlorine and temperature of the water in all studied pools were 7.6, 1.5 mg/L and 31.2 °C, respectively. Turbidity was not observed in any water samples. Totally, 29 fungal species were identified from water and the environment of the pools. Chlorine level was found to be associated with the fungi isolated. Different species of filamentous hyaline hyphomycete (Aspergillus, Penicillium), pheohyphomycete (Alternaria, Epicoccum and Cladosporium) and Yeast (Candida, Rhodotorolla) were isolated. The changing rooms and shoe racks were most contaminated parts of the pools. Conclusion: Given the isolated different fungal species from water and environment of the swimming pools, cleaning the environment of the pools, especially changing rooms and shoe racks, and control of water chlorine level, play an important role in the reduction of fungal contamination and transmission.  }, Keywords = { Swimming Pools, Saprophytic fungi, Dermatophytes, Chlorine, Cladosporium}, volume = {8}, Number = {3}, pages = {870-880}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1533-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1533-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {aghaeiborashan, fatemeh and Shahrooz, rasoul and Ahmadi, abbas and MazaheriKhamene, ramin and Imani, mehdi}, title = {Histomorphometrical study of the effect of platelet-rich plasma therapy on the damage caused by the Torsion-Detorsion testicular in mice}, abstract ={Background & Objectives: Testicular Torsion is one of the emergency cases which is caused by twisting of the spermatic cord; Detorsion leads to the recirculation of blood and ultimately, more damage occurs in testis. Platelet-Rich plasma (PRP) contains several growth factors and cytokines that can restore tissue and rebuild cells, so in this study, PRP was used to evaluate its effects. Materials & Methods: Twenty-eight adult male mice were randomly divided into three equal groups (n = 6). The first group, is the healthy control, without testicular twisting. Group II: Torsion Detorsion (TD) + PBS (phosphate-buffer-solution) group, Group III: The TD + PRP group. In these two groups spermatic cord were twisted for one hour, and after detorsion, PBS and PRP were injected into the rete testis, respectively. 35 days after surgery, left testis was sampled for histomorphometrical examinations. Obtained data were statistically compared by ANOVA and post hoc-Tukey test (P <0.05). Results: Obtained data from the mean thickness of germinal epithelium, diameter of seminiferous tubules (P<0.01), and, the spermatogenesis indices (P<0.001) were decreased significantly in TD+ PBS group. The thickness of the testis capsule and the lumen of seminiferous tubules showed a significant increase (P<0.01). While, PRP significantly improved these parameters (P<0.01). Conclusion: Testicular TD causes many impacts and disorganization in the male reproductive system, and resulting infertility. It seems that, PRP due to possession ample of growth factors, able to relatively improve the undesirable effects of TD.  }, Keywords = {Testicular Torsion-Detorsion, Platelet Rich Plasma, Histomorphometry, Testis, Mice}, volume = {8}, Number = {3}, pages = {881-889}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1550-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1550-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {golbahari, khadije and AbtahiFroushani, Meysam}, title = {Investigating the effects of Thymol on animal model of Rheumatoid Arthritis}, abstract ={BBackground & Objectives: Nowadays, attention to natural products with therapeutic potential has been increased. This study was carried out to investigate the effect of Thymol on the animal model of Rheumatoid arthritis (RA). Materials & Methods: RA was induced by injection of complete Freund's adjuvant into the footpad of Wistar rats. Then, rats were allocated in 3 groups: treated with Thymol (100 mg/kg-orally), treated with Prednisolone (100 mg/kg-orally) and un-treated group. All treatments were initiated at the 5th day after induction. The change in the dorso-plantar diameter of hands and legs of each rat were recorded every other day until 23 days after induction. Results: The edema and swelling of the soles of the feet of RA rats that received Thymol or prednisolone, were significantly decreased in a similar manner compared to RA rat without treatment. The serum levels of nitric oxide and myeloperoxidase correlated with the proliferation of spleen lymphocytes and the levels of IL-1 and IL-6 production were significantly decreased in treatment groups compared to control group rats. The level of decrease in the level of serum nitric oxide was higher in the thymol group compared to the prednisolone-induced group. Conversely, prednisone caused more decrease in the levels of IL-1 and IL-6 cytokines compared to RA rats that received thymol. Conclusion: Thymol may be considered as a useful strategy to control RA disease.}, Keywords = {Thymol, Rheumatoid Arthritis, Immunity Responses}, volume = {8}, Number = {3}, pages = {890-900}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1557-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1557-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Seyed, Asiye and Farsi, Sirous and Hosseini, Seyed Ali and Kaka, Gholamrez}, title = {Antioxidant Effects of Swimming Training and Curcumin in Withdrawal Period of Alcohol Overdose in Rats}, abstract ={Background & Objective: Alcohol overdose can induce increment in free radicals. The present study aimed to  investigate the effect of swimming training and Curcumin in withdrawal period of alcohol overdose on superoxide dismutase (SOD), glutathione peroxidase (GPX), total antioxidant capacity (TAC), malone dealdeid (MDA) and protein carbonyl (PC) of rats. Materials & Methods: 40 rats were selected and administered alcohol every 8 hours for 4 days. Then they were subjected to the withdrawal of alcohol for six days and in seventh day divided in 5 groups of 8 rats (1) control (2) Curcumin (3) swimming training (4) Curcumin and swimming training (5) sham. Groups 3 and 4 swam 5 sessions per week for 2 weeks and groups 2 and 4 used Curcumin 5 times per week for 2 weeks peritoneally. For statistical analysis of data two-way ANOVA was used (p≤0.05). Results: Swimming training had significant effect on increase of SOD (p=0.001), GPX (p=0.001) and TAC (p=0.009) and reduction of MDA (p=0.001) and PC (p=0.001) of rats. Curcumin had significant effect on increase of SOD (p=0.001) and GPX (p=0.001) and reduction of MDA (p=0.001) and PC (p=0.001) of rats but had no significant effect on TAC (p=0.34) also Curcumin and swimming training had interactive effects on increase of SOD (p=0.001) and GPX (p=0.001) and reduction of PC (p=0.009) of rats but had no interactional effect on TAC (p=0.48) and MDA (p=0.13). Conclusion: Probably, swimming training and curcumin can be applied simultaneously in withdrawal period of alcohol overdose to increase antioxidant factors.}, Keywords = {Swimming training, Curcumin, Antioxidant, Alcohol}, volume = {8}, Number = {3}, pages = {901-910}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1555-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1555-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Javanmard, Rahele and mahdavi, Sam}, title = {Investigating Antibacterial Effect of Thyme (Thymus persicus) and Pennyroyal (Mentha longifolia) Alcoholic and Aqueous Extracts against Isolated Bacteria from Domestic Cheeses}, abstract ={Background & Objective: Food poisonings caused by consumption of domestic dairy products such as cheese due to Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus) are one of the hygienic problems in foodstuffs. Nowadays, using natural preservatives instead of synthetic preservatives in foods has been regarded increasingly and among these natural compounds, medicinal plants comprise these materials that finding their antimicrobial effects have high priority. The aim of this study was to investigate antibacterial effect of Thyme (Thymus persicus (and Pennyroyal (Mentha longifolia) alcoholic (ethanolic) and aqueous extracts against isolated bacteria from domestic cheeses. Material & Methods: After preparing Thyme and Pennyroyal, alcoholic (ethanolic) and aqueous, the extract of these plants were gained and their antimicrobial effects were investigated in concentrative levels of 0.39- 100 percent against E. coli and S. aureus isolated from domestic cheese by microdilution method. E. coli PTCC 1270 and S. aureus PTCC 1112 were used as control. Results: Alcoholic (ethanolic) extract of Thyme and Pennyroyal showed more antibacterial effect against Staphylococcus aureus in comparison with Escherichia coli (P<0.05). Thyme ethanolic extract showed more antibacterial effect than Pennyroyal ethanolic extract (P<0.05). Aqueous extract of Thyme and Pennyroyal did not show any significant antibacterial effect in tested concentration (P>0.05). Conclusion: According to current study results, alcoholic (ethanolic) extract of Thyme and Pennyroyal have high antibacterial potency and they can be used as alternative instead of synthetic preservatives for protection against food borne bacterial poisoning and infection.}, Keywords = {Extract, Thymus persicus, Mentha longifolia, Antibacterial, Microdilution}, volume = {8}, Number = {3}, pages = {911-917}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1563-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1563-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {mousavi, mohammad and milajerdi, alireza and HassanzadehKeshteli, Ammar and Esmaillzadeh, Ahmad and afshar, hamid and adibi, peym}, title = {Investigating the relationship between Dietary total antioxidant capacity and depression and anxiety in Iranian adults}, abstract ={Background & Objective: Although several studies are available linking individual dietary antioxidants to the risk of psychological disorders, data about the association of dietary total antioxidant capacity (TAC) with depression and anxiety are scarce. This study was conducted to investigate the association between dietary TAC and odds of depression and anxiety among Iranian adults. Material & Methods: In this cross-sectional study, dietary intakes of 3297 apparently healthy adults in Isfahan province, Iran, were assessed using a validated detailed food frequency questionnaire. Dietary TAC was estimated using the ferric-reducing antioxidant power (FRAP) international databases. Depression and anxiety were assessed using a validated Persian translated version of Hospital Anxiety and Depression Scale (HADS) questionnaire. Having the scores 0-7 in either subscales was considered as “normal” and the scores of 8 or more as the presence of depression or anxiety. Results: Overall, 28.6% (n= 943) of study participants were depressed or at the borderline level, and 13.6% (n= 448) had anxiety or were at the borderline level. After controlling for potential confounders, including dietary intakes of omega-3 fatty acids, we found that those in the top quintile of dietary TAC were 43% less likely to be depressed than those in the bottom category (OR: 0.57; 95% CI: 0.40-0.82, Ptrend= 0.001). Regarding anxiety, there was a significant inverse association between dietary TAC and anxiety comparing extreme quintiles (OR: 0.62; 95% CI: 0.39-0.99, Ptrend= 0.06). Conclusion: A significant inverse association between dietary TAC and odds of depression and anxiety was found among adults. Further prospective studies are required to confirm these findings.}, Keywords = {Anxiety, Depression, Dietary total antioxidant capacity, Iran, Mood }, volume = {8}, Number = {3}, pages = {918-928}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1564-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1564-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {AbtahiFroushani, Seyyed Meysam and Mohammadi, Vahi and Tehrani, Ali Asghar and EsmaeiliGouvarchinGhaleh, Hadi and MansoriMotlagh, Bahm}, title = {Therapeutic effects of hydro-alcoholic extract of Cynodon dactylon in hypercholesteremic rabbits}, abstract ={Background & Objective: This study was carried out to investigate the anti-atherosclerotic properties of hydro-alcoholic extract of C. dactylon in hypercholesterolemic rabbits. Materials & Methods: The standard regime was consisted of 5 normal rabbits that they were fed for 75 constitutive days with a standard regimen. Hypercholesterolemic group was consisted of 20 rabbits that were fed for 75 constitutive days with a high cholesterol (1% of food weight). These rabbits were allocated in 4 equal groups at the day after initiating the research, including animals with the hyper-cholesterol regime and treatment groups that daily received 100,200 or 400 mg/kg of the hydro-alcoholic extract of C. dactylon, respectively. Results: On the 45th and 75th day after beginning, other than blood sugar, the levels of triglyceride, total cholesterol, HDL and LDL, atherogenic index (LDL/HDL ratio), CRP, fibrinogen, serum levels of nitric oxide and malondialdehyde were significantly increased in rabbits with hypercholesteremic regime and without treatment compared to normal rabbits. The oral administration of the extract of C. dactylon at all doses for 30 constitutive days after the establishment of hypercholesterolemia, could significantly reduce the levels of cholesterol, triglyceride, LDL, and malondialdehyde. Moreover, 200 and 400 mg/kg of extract could significantly decrease the levels of the atherogenic index, CRP, fibrinogen, and malondialdehyde, and simultaneously increase the levels of HDL compared to rabbits with hypercholesterolemia. Only 400 mg/kg of extract could significantly decrease the serum levels of nitric oxide. Conclusion: Hydro-alcoholic extract of C. dactylon showed beneficial effects in reducing of hypercholesterolemia in the animal model.}, Keywords = {Cynodon dactylon, Atherosclerosis, hypercholesteremic, Rabbit}, volume = {8}, Number = {3}, pages = {929-937}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1566-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1566-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Jebelli, Asiyeh and Khalaj-Kondori, Mohammad and Bonyadi, Mortaza and HosseinpourFeizi, Mohammad Ali and Rahmati-Yamchi, Mohamm}, title = {Investigating Quantitative analysis of the gene expression of calcium/calmodulin-dependent protein kinase IV by the effect of Olibanum alcoholic extract in PC12 cell line}, abstract ={Background & Objective: Long-term memory depends on protein synthesis. The product of Camkiv gene promotes memory via activating its proteins. The treatment of laboratory animals by Olibanum leads to memory improvement and the recovery of Alzheimer. Therefore, the aim of this study is the evaluation of Olibanum ethanolic extract on the Camkiv expression in PC12 cells. Materials & Methods: Olibanum toxicity on the cell viability was investigated by MTT test. Cells were treated with concentrations 10,25,40,55,70 and 85 μg/ml of extract in time intervals 12,24,48 and 72 hours and their absorption rate was measured. Then, cells were treated by concentrations 2 and 20 μg/ml of extract in mentioned times. Extracted RNA was converted into cDNA and real-time PCR performed. Results: Cell death was raised by increasing time and concentration of extract treatment. IC50 values were obtained as 71.01, 52.95, 21.05 and 13.85 μg/ml in 12, 24, 48 and 72 hours of treatment, respectively. Besides, concentrations 2 and 20 μg/ml significantly increased Camkiv expression following 24 hour treatment. The maximum expression of Camkiv was observed in 48 hour treatment. The effect of Olibanum on gene upregulation was stable until 72 hours. Conclusion: The Olibanum ethanolic extract can remarkably upregulate Camkiv expression for a long time. These results are consistent with the previous studies indicating the effect of Olibanum on upregulation of Bdnf, Camkiv‌-downstream gene. However, regarding the existence of the two-direction pathway in the expression regulation of Bdnf and Camkiv‌, comprehensive studies are required to determine exact mechanism of Olibanum function in the brain.  }, Keywords = {Olibanum ethanolic extract, Camkiv gene, PC12 cells}, volume = {8}, Number = {3}, pages = {938-948}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1571-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1571-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {roeintan, abozar and FadaeiNobandegani, Fatemeh}, title = {Design, Molecular Docking Studies and Toxicity Prediction of Some Novel 1, 2, 3-Triazole Derivatives Containing Piperazine Moiety as Antifungal Agents and CYP-51 Inhibitors}, abstract ={Background & Objective: In this study, a number of new triazole derivatives, containing a 1, 2, 3-triazole ring attached to the piperazine moiety as antifungal agents and lanosterol 14 alpha-demethylase, (CYP51) inhibitors were docking studies conducted. In the following, the toxicity risks of the designed compounds, were predicted by existing software. Materials & methods: Initially, the chemical structures of all azole were designed using ChemBioDraw Ultra14.0 program, then transferred into Hyperchem software for energy minimization. After preparing, all of these chemical compounds were docked with the target enzyme in order to select the best inhibitor of the drug using the Auto Dock-Vina-1-1-2-win32.msi software. The results were analyzed using the Molegro Virtual Docking software. At the final stage, the toxicity risk prediction of compounds was performed by the OSIRIS program. Results: After checking the computation, 10 compounds of ligands that were the results of Docking, were selected according to the Gibbs free energy (least ΔG). Docking results revealed the azole-heme coordination, hydrogen bond, hydrophobic interactions were involved in the drug-receptor interactions. Among the all studied compounds, the best docking results were related to No. 5 displayed. In fact, this compound had the most negative ΔGbind (-10.85 Kcal/mol) that indicated favorable interactions with the key amino acid residues at active site of CYP51. Conclusion: In conclusion, according to the results of docking studies, biological evaluation and Toxicity Risk Prediction of designed Compounds, it can be concluded that Compound No. 5 can be considered as an effective antifungal agent and an inhibitor of the CYP51 enzyme   }, Keywords = {Molecular Docking, Toxicity Risk Prediction, CYP51 Inhibitor, 1, 2, 3-triazole, Antifungal}, volume = {8}, Number = {3}, pages = {949-958}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1572-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1572-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Hedayat, Behnam and Bahmanyar, Maryam and Amini, Shahrz}, title = {Investigating Characteristics Data of Diabetes Mellitus in Children under 18 Years old, in Vali-Asr Hospital, Fasa}, abstract ={ Background & Objective: Despite evidence of an increase in the incidence of diabetes in youth, there are few characteristic data in diabetic patients in the Islamic Republic of Iran. The present study sought to investigate the Characteristic data of childhood diabetes in a University Hospital in Iran. Materials & Methods: Hospital admission data were obtained from discharge database of Vali-Asr tertiary care teaching hospital in the capital city of Fasa, Iran. Accordingly, 45 patients younger than 18 years old diagnosed with diabetes during the period of March 2014 to June 2016, were selected to participate in the study. World Health Organization (WHO) registry criteria and SPSS software version 13 were used to study and analyze the data. Results: The results indicated that nearly half of the children presented with ketoacidosis in which most of them were in the mild or moderate group. Positive family history of diabetes was found in 22.2% of the patients. Conclusion: Theoretically, it seems the frequency of diabetic ketoacidosis (DKA) in type 1 diabetic children was significant in this region of Iran. In addition, it was observed that polydipsia, polyuria, weakness, and fatigue were the most frequent clinical symptoms reported during the diagnosis.}, Keywords = {Diabetes type 1, Diabetes type 2, Diabetic ketoacidosis}, volume = {8}, Number = {3}, pages = {959-966}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1675-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1675-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {MajidiPoya, Mousa and Khodavandi, Alirez}, title = {Comparison of the Antifungal Activity of Honey and Fluconazole against Candida albicans in vitro and in an Enteric Candidiasis Mouse Model}, abstract ={Background & Objective: Candidiasis has gained importance due to its increasing prevalence in immunocompromised patients. Antifungal drugs such as fluconazole and amphotericin B are used for the treatment of candidiasis. One of the biggest problems faced in clinical practice is resistance for most of these drugs. The antifungal drugs derived from natural products have helped to overcome this problem. This study evaluated the effects of Kohgiluyeh and Boyer-Ahmad natural honey and fluconazole on C. albicans in an enteric candidiasis mouse model. Materials & Methods: The in vitro antifungal activity of honey samples from different sources were evaluated for their ability to inhibit the growth of C. albicans. Time kill test was carried out to evaluate the anticandidal activities of the honey tested. Eventually, the efficacy of the best honey tested compared with fluconazole against C. albicans was evaluated in vivo through an enteric candidiasis mouse model. Results: The results revealed that the honey tested was able to inhibit C. albicans. Our data indicated that oak honey largely represented antifungal activity among the honey tested (p≤ 0.001). Treatment of BALB/c mice infected with C. albicans showed that treatment with honey was slightly less efficacious than fluconazole treatment in terms of the fungal load reduction, it was still effective against C. albicans. Conclusions: These results demonstrate the efficacy of anticandidal effects of honey extracts both in vitro and in an animal model of candidiasis and affirm the potential of honey to be used as an adjuvant therapy in the management of Candida infections.}, Keywords = {Candida albicans, enteric candidiasis, honey}, volume = {8}, Number = {3}, pages = {967-978}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1637-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1637-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {}, title = {Assessing the trend of mortality and its causes in patients admitted to intensive care unit of Shahid Sadoughi hospital in Yazd between 2008 and 2013}, abstract ={Background & objectives: This study aimed to assess the causes of hospitalization and mortality trend among hospitalized patients in intensive care unit (ICU). Materials & Methods: In a cross-sectional study, all patients admitted to ICU of the hospital between 2008 and 2013 were investigated in terms of demographic characteristics, reasons for hospitalization and the cause of mortality. The data were collected from patient’s medical records and were analyzed using SPSS 21 software. Results: 54.8 % were male and 45.2 % female out of the 1520 patients. The patients’ mean age was 56.8 ±26.9 years. The most common causes of hospitalization were stroke (12.8%), cancer (11.8 %) and septicemia (7.5 %) respectively. Most patients died from circulatory system diseases (41.1%) and infections (26.8%). The mortality risk was statistically correlated with older age. Conclusion: Based on the results, mortality has been raising and directly correlated with age.}, Keywords = {Intensive Care Unit, Mortality, Trend}, volume = {8}, Number = {3}, pages = {979-985}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1528-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1528-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {RefaeeSaeedi, nasrin and aghamohammadiansharbaf, hamidreza and AsghariEbrahimAbad, Mohammad Javad and Kareshki, Hossei}, title = {Psychological Consequences of Breast Cancer in Iran: A Systematic review}, abstract ={Background & Objective: Diagnosis of breast cancer and related therapies are emotional experiences for women. The present study aimed to identify the psychological consequences of breast cancer in previous studies based on systematic review. Materials & Methods: The present study is a descriptive survey and, according to the method of implementation, is a systematic review study. A literature search was conducted by using following electronic databases including scientific information databases (SID), Magiran, Medlib, Sciencedirect, PubMed, Google Scholar, Web Of Science, Scopus, cinahl and medline from 1991 through 2017 regarding the psychological consequences associated with breast cancer in iran Using keywords cancer, mood disorder, anxiety disorder, mental disorder, life quality, sexual dysfunction, sleep disorder, mental hygiene, insomnia, anxiety, body image, mental disorder, behavioral symptom, psychological distress, coping behaviors, traumatic stress disorder and breast cancer. The content of all articles, after extraction from the bases, was evaluated by the prisma checklist and the Content analysis was used to examine data Results: After the search and evaluation of the studies, the final analysis was carried out on 73 articles. The findings of this study showed that the psychological consequences of breast cancer in 8 categories are quality of life, anxiety, stress and stress, depression, sexual function, body image, coping strategies, fatigue and quality of sleep. Conclusions: Based on the results, drawing on the areas identified in the field of psychological outcomes of breast cancer can be used as a conceptual map for more coherent studies and the design of extensive interventions.    }, Keywords = {Psychological Consequences, Breast Neoplasms, Systematic Review}, volume = {8}, Number = {4}, pages = {986-999}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1727-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1727-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Golestani, Ali and AbdollahipourHaghighi, Raziyeh and Takhshid, Mohammad Ali and Arabsolghar, Rit}, title = {New techniques and methods in the study of the invasion, cell migration and MMPs activity in vitro and in animal models}, abstract ={Background & Objective: Cancer metastasis is the primary cause of cancer morbidity and mortality, it accounts for about 90% of cancer deaths. Cancer treatment has improved significantly, due to early detection and inhibition of cancer growth. The ability to invade and migrate is important in malignant tumor cells. The study of cell migration is valuable in cancer diagnosis, prognosis, drug development and treatment. New methods are available to investigate the invasion, migration and the activity of enzymes involved in the invasion process in the laboratory. The effectiveness and efficacy of various anti-cancer drugs and compounds can be studied using these methods in laboratory and animal models. Conclusion: In this paper, we offer a summary of in-vitro migration assays, including the transwell migration assay, scratch wound assay, microfluidic chamber assay, exclusion zone assay, fence assay, micro carrier bead assay, spheroid migration assay and in-vivo approach, Chick chorioallantoic membrane (CAM) assay. This review also provides an overview of methods, In situ Zymography, ELISA, and FRET based measurement of MMP activity and Substrate Zymography for measuring the level of metalloproteinase enzyme as the major enzyme in the degradation of extracellular matrix.  }, Keywords = {malignant tumor, matrix metalloproteinase enzyme, cell migration assays}, volume = {8}, Number = {4}, pages = {1000-1011}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-791-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-791-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Ahanjan, masoumeh and AbtahiFroushani, meysam and AfzalAhangaran, N.A}, title = {Investigating the effects of RAW264.7 macrophages treated with melatonin on the erythroleukemia cell line K562}, abstract ={Background & Objectives: Macrophages have a variety of functions and different phenotypes that are affected by the microenvironment. Due to the multiple functions, Melatonin can promote the cancer progression or cancer regression in relation to other factors. However, there is no information about the role of melatonin on the formation of macrophages in the tumor environment. The purpose of the present study is to evaluate the effects of melatonin treated RAW264.7 macrophages on the growth rate of erythroleukemia cell line K562.  Materials & Methods: RAW264.7 cells were cultured in the 24-well plates at a density of 6 × 104 cells per well. After culturing for 24 h, the cells were treated for 24 h with melatonin at concentrations 0, 50, 75, 100, 150, 200 μmol/L. After removal of the supernatant, macrophages and K562 were co-cultured in 1:10 ratio. K562 vitality was then determined by MTT and NR assay. The rate of apoptosis in the cell population was evaluated by staining with acridine orange and ethidium bromide colors. Also, NO and MPO were measured in the supernatant of macrophages RAW264.7. Data were statistically analyzed using Kruskal-Wallis and Mann–Whitney U test (P<0.05). Results:  The growth rate and vitality of K562 cells co-cultured with melatonin-treated macrophages was increased at concentrations of 50 and 75 μmol/L. Nevertheless, at higher concentrations of melatonin, this process was reversed. The levels of MPO and NO were decreased by increasing concentrations of melatonin. Conclusion: These results indicated that treatment of macrophage with melatonin, especially at lower doses (50 and 75μmol/L) could remarkably increase the K562 viability and vitality.}, Keywords = {Cytotoxicity, Melatonin, RAW264.7 Macrophage, K562}, volume = {8}, Number = {4}, pages = {1012-1024}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1695-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1695-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Khoobbakht, Dorna and Amani, Jafar and Zare, Shohreh and Nourmohammadi, Zahr}, title = {Cloning and evaluation of gene expression and purification of gene encoding recombinant protein containing binding subunit of coli surface antigens CS1 and CS2 from Enterotoxigenic Escherichia coli}, abstract ={Background & Objective: Enterotoxigenic Escherichia coli (ETEC) is a major causative agent of diarrhea. Enterotoxins and the colonization factors (CFs) are major virulence factors in ETEC infections. The bacterium binds to the intestinal epithelial cell surface through colonization factors and produces enterotoxins that cause excessive fluid and electrolyte secretion in the lumen of the intestine, which ultimately leads to diarrhea. Due to the difficult of treatment and the high prevalence of this disease, the design of the vaccine against this organism is one of the goals of the World Health Organization. The CooD-CotD protein, as adhesion tip subunits of CS1 and CS2, plays an important role in bacterial attachment to the intestinal epithelial cells. In this study, the expression and purification of chimeric protein CooD-CotD was carried out with the aim of investigating as candidate vaccine. Materials & Methods: In this study, codon optimization of cooD-cotD chimeric gene was performed by Gene Designer software. The gene was amplified by PCR and cloned into pJET1.2 / blunt then it was subcloned into pET28a to express chimeric protein. Recombinant protein was purified following expression, using Ni-NTA affinity chromatography and confirmed by western blotting analysis. Results: The presence of 82kDa in the SDS-PAGE gel showed that expression of CooD-CotD chimeric protein and confirmed by western blotting analysis. Yield of purified protein was 121 mg/ml. Conclusions: Expression and protein purification studies showed that this protein has expression in the homologous host.}, Keywords = {Enterotoxigenic Escherichia coli, CS1, CS2, Recombinant expression}, volume = {8}, Number = {4}, pages = {1025-1035}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1770-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1770-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Fatahi, Somaye and Kordvarkaneh, Hamed and Azadbakht, Leil}, title = {Association between dietary acid load with alternative Mediterranean diet and dietary approaches to stop hypertension among Tehranian women}, abstract ={Background & Objective: There are few studies that have been examined the association between dietary acid load and healthy diet patterns. The purpose of this study was to evaluate the association between dietary acid load with alternative Mediterranean diet score (aMED) and dietary approaches to stop hypertension (DASH). Matrrials & Methodes: This cross-sectional study was conducted among 270 women with age range 20-50 years in Tehran, Iran. Usual dietary intakes were assessed by a semi-quantitative food frequency questionnaire, which contains 168 food items. Potential renal acid load (PRAL) and Net Endogenous Acid Production (NEAP) and Dietary acid load (DAL) was calculated for each person. The scores of aMED and DASH were estimated for participants. Results: The mean ± SD of age was 32.64 ±8.44 years. AMED scores were significant difference among the PRAL, NEAP and DAL tertiles (P<0.001, P=0.01 and P=0.003 for PRAL, NEAP and DAL respectively). Also, the women who were in the middle tertiles of dietary acid load indices, have lower DASH scores compared with the lower tertiles (P=0.007, P=0.03 and P=0.005 for PRAL, NEAP and DAL respectively). These results stayed significant even after adjusting for multiple covariates. Conclusion: The present study showed that dietary acid load indices have a negative association with aMED and DASH scores.    }, Keywords = { Dietary acid load, alternative Mediterranean diet, Dietary approaches to stop hypertension}, volume = {8}, Number = {4}, pages = {1036-1045}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1629-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1629-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Jafarzadeh, Saeedeh and fereidouni, armin and khiyali, zahr}, title = {Evaluation the Quality of Clinical Education from Perspectives of perating Room Students, in Fasa University 2016}, abstract ={  Background & Objective: Clinical teaching, learning support activities in clinical environments Which aims to achieve student learning goals for clinical care and good infrastructure to align theoretical knowledge to practical skills, So that the current state of knowledge can improve the quality of students admitted to the operating room to provide in the future. Thus the aim of this study was to evaluation the quality of clinical education from Perspectives of Operating Room Students Materials & Methods: This descriptive and cross-sectional study was conducted on 81 operating room students of Fasa University of Medical Sciences Using consensus sampling method in 2015 -2016. Data were collected using a valid questionnaire including demographic variables and assessing the viewpoints of students about clinical education. Data were analyzed using descriptive statistics in SPSS v. 22. Results: In this study, 22 (27.2%) men and 59 (72.8%) were female. According to study results from the perspective of students (37%) confidence clinical instructors to students, 6/55% to prepare students to enter the clinical setting, 48.1% confidence and willingness of teachers to students and to students, 55.6% Prepare students to enter the learning, 50.6% confidence and a positive attitude to education, 44.6% Personnel familiar with the professional ethics and42% Adverse environmental features. Conclusion: Based on the results of this study, clinical education in the operating room has problems and shortcomings that needs to be addressed by authorities, professors and clinical education experts in order to resolve existing problems and weaknesses.  }, Keywords = {Clinical education, Operating Room, Students, Evaluate, Perspectives }, volume = {8}, Number = {4}, pages = {1046-1055}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1640-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1640-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Kheirandish, Rezvan and Ranjbar, Rouhollah and Veisi, Ali}, title = {The response of Irisin Serum and insulin resistance to acute Pilates training sessions Pilates Training in obese sedentary women}, abstract ={Background & Objective: Irisin is myokine, that is secreted by acute exercise activity from the muscle and increases energy consumption. The purpose of this study was to determine the response of irisin serum and insulin resistance to acute pilates training sessions pilates training in obese sedentary women. Materials & Methods: This research is a semi experimental study with pre-and post-test design. The statistical population of this study was made up of disabled obese women in Ahwaz, with 21 subjects (mean age 39.1±10.0 years and BMI 35.9±3.4 kg/m2) were randomly divided into Pilates group (n=12) and control group (n=9). The training group performed 60-minute Pilates training with a14-16 Borg perceived perception index, while the control group did not exercise. After at least 8 hours-fasting, blood samples were collected before and immediately after each activity, to measure the levels of Irisin, glucose, insulin and insulin resistance. Data were analyzed with paired-sample t-test and ANCOVA and the relationship between the variables was calculated by Pearson correlation test. Results: The results indicated no significant changes in blood glucose, insulin, insulin resistance index and serum Irisin levels in Pilate’s group compared with the control group after a Pilates exercise session (p≥0.05). Furthermore, correlation was not between Irisin to, glucose, insulin, and insulin resistance index (p≥0.05). Conclusion: Although research on this regard is a beginning pathway. However, Acute Pilates exercise does not seem to have an effect on glucose, insulin, insulin resistance, and Irisin level.    }, Keywords = { Irisin, Acute Pilates training, Obesity, Insulin Resistance }, volume = {8}, Number = {4}, pages = {1056-1067}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1634-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1634-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Sadeghipour, Hamid Reza and Salesi, Mohsen and Rabiezade, Alirez}, title = {Effects of 4 week endurance training on PGC-1α expression in adipose tissue, ANGPTL8 serum concentrations and beta cells function of STZ diabetic rats}, abstract ={Background & Objective: Angiopoietin-like Proteins 8 (ANGPTL8) which secreted form adipose tissue due to downstream PGC-1α pathways, is the main factor for regeneration of beta cell. The aim of this study was to investigate the effect of 4 endurance training program on PGC-1α expression in adipose tissue, ANGPTL8 serum concentration and beta cell function (HOMA.B) in diabetic rats. Materials & methods: Male Wistar rats (N=24) divided in 3 groups include healthy control (HC), diabetic control (DC) and endurance training (ET). After induction of diabetes with STZ, training groups performed 4 weeks endurance training and real-time polymerase chain reaction (Real-Time PCR) method used for the relative expression of PGC-1α in visceral adipose tissue, the enzyme-linked immunosorbent assay (ELISA) method for measuring serum ANGPTL8 levels. Also beta cell number was counted with pancreases hematoxylin-eosin evaluation. Results analyzed with ANOVA. Results: The relative expression of mRNA PGC-1α was significantly increased in ET (P=0.001). Although ANGPTL8 levels increased in ET group, this change wasn’t significant (P=0.47). HOMA.B didn’t showed any significant change in ET (P=.0.08) but the number of beta cells in this group significantly increased (P=0.001). There was a positive correlation between relative mRNA PGC-1α and ANGPTL8 levels. Conclusion: Despite the positive and significant correlation between relative expression of mRNA PGC-1α and ANGPTL8, this increase wasn’t significant, but this could increases the number of beta cell in endurance training group. Further studies with differences training programs are recommended.  }, Keywords = {Endurance Training, Beta Cells proliferation, PGC-1α, ANGPTL8}, volume = {8}, Number = {4}, pages = {1068-1078}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1635-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1635-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {roostaee, farzad and Zanganeh, shima and Shafiepour, Mohammad Reza and Mahmoudi, Mehdi and khoshdel, Alireza and hajizadeh, Mohammad Rez}, title = {The Survey of relationship between Serum Resistin Level and Metabolic Syndrome Components in patients with and without this syndrome in Rafsanjan (2015)}, abstract ={Background &Objective: Resistin is an adipokin secreted from adipose tissue and immunity cells. Relationship between resistin and metabolic syndrome and its components is controversial. The aim of this study is measurement of serum resistin level, insulin resistance and other factors in subjects with and without metabolic syndrome. Materials & Methods: this cross-sectional study was conducted in 2015, 56 availableperson including; 31subjects were enrolled as the study group and 25 subjects were recruited as the control group of the study. Serum resistin concentrations were measured by ELISA and its association with metabolic syndrome markers was measured. Data were Analyzed using independent T test, Pearson correlation Test and, Chi-squared Test Results: Serum resistin levels were significantly higher in the metabolic syndrome subject compared with control group (p-value=0.002). Correlation analysis revealed body mass index (p=0.018; r=0.480), waist circumference (p=0.015; r=0.431), systolic blood pressure (p=0.008; r=0.467), diastolic blood pressure (p=0.001; r=0.562), cholesterol (p=0.018; r=0.421) and LDL (p=0.003; r=0.515) were positively correlated with plasma resistin. Conclusion: Serum resistin levels are elevated in subjects with metabolic syndrome and are associated with some of its components in metabolic syndrome patients, resistin may be associated with the severity of this syndrome and its complications.    }, Keywords = {Resistin, metabolic syndrome, Insulin Resistance}, volume = {8}, Number = {4}, pages = {1079-1087}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1277-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1277-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Edalatmanesh, Mohammad Amin and Amooei, Khadijeh}, title = {The lithium effect on learning and memory deficits and hippocampal level of BDNF in prenatal seizure kindled rats}, abstract ={Background & Objective: Prenatal seizures cause neuronal damage in the limbic area of fetal brain leading to learning deficits and cognitive impairment in newborns. This study examined the effect of prenatal tonic-clonic seizure and lithium chloride (LiCl) therapy on the learning and memory of rat’s pups. Materials & Methods: Pregnant Wistar rats were kindled by (i.p) injections of 40 mg/kg/BW of Penthylentetrazole (PTZ) on embryonic day (ED) 13 for 7 consecutive days. LiCl treated rats in PTZ+Li0.04, and PTZ+Li0.08 groups received 0.04% and 0.08% of LiCl in tap water from ED10 to postnatal day (PND) 24, respectively. The spatial performance learning was evaluated at PND 30 using Morris water maze (MWM). After behavioral study, the hippocampal level of BDNF was measured using ELISA. Results: In comparison to controls, PTZ group showed a significant increase in latency time and distance to hidden platform in MWM test and decrease level of hippocampal BDNF. LiCl-treated rats showed a significant lower latency time and distance to hidden platform in MWM and higher level of BDNF than PTZ group. Conclusion: The LiCl with elevated of BDNF in hippocampus can ameliorate spatial memory in prenatal seizure model.    }, Keywords = {Seizure, Lithium, Pregnancy, Rat }, volume = {8}, Number = {4}, pages = {1088-1096}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1592-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1592-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Fathollahi, Fazlollah and Faramarzi, Mohammad and Hemmati, Rouhollah}, title = {The Effect of 10 weeks of high-intensity exercise training on resting levels of some angiogenesis and pulmonary function of men with prostate cancer}, abstract ={ Background & Objective: Prostate cancer is the most common malignancy and the eighth cause of death in Iran. Angiogenesis is the major cause of metastatic and drug resistance in this cancer. The aim of this study was to investigate the effects of 10 weeks of high intensity periodic exercises on resting levels of some angiogenesis markers and also the pulmonary function of men with prostate cancer. Materials & Methoed: 20 male patients with prostate cancer aged 55 to 62 years old were randomly divided into two groups (n = 10) and experimental (n = 10). The experimental group subjected to 10 weeks of periodic exercise with intensity progressed weekly, three sessions for 40 minutes and intensity of 70-75 was set for each session. 48 hours before and after exercise, the serum levels of VEGF, FGF, nitric oxide (NO) and also pulmonary function were measured. Intra and intergroup changes were analyzed using dependent t-test and independent t-test, respectively. Results: The results showed that 10 weeks of severe periodic training significantly increased the levels of VEGF (P = 0.001), FGF (P = 0.001) and NO (P = 0.001) in the experimental group compared with the control group. Moreover, considering the results, FVC in patients with periodic exercise has increased significantly (P = 0.002). Conclusion: As for results, it can be concluded that 10 weeks of periodic exercise increases the angiogenesis and pulmonary function in patients suffered from prostate cancer.    }, Keywords = {High-intensity interval training, prostate cancer, angiogenesis markers, pulmonary function}, volume = {8}, Number = {4}, pages = {1097-1105}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1671-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1671-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {roeintan, abozar}, title = {Biological Evaluation of Heterocycle Moiety of Some Novel azoles Derivatives as Antibacterial and Antifungal potential Agents}, abstract ={Background & Objective: Azole nucleuses are very important part of antimicrobial, analgesic and anti-inflammatory drugs. The azole class of compounds is the most popular among the antibacterial and antifungal classes because of its lower toxicity, higher efficacy and a broad spectrum of activity. Today, Efforts have focused on the development of new, less toxic and more efficacious antifungal and antibacterial drugs, with the novel mechanism of action. In this study, we tried to investigate the antibacterial and antifungal effects of the new azole derivatives. Materials & methods: This research is a descriptive – cross sectional study. The antibacterial and antifungal activity of compounds was evaluated using the minimum inhibitory concentration (MIC) method on a series of gram positive and gram negative bacterial strains and Aspergillus niger, Aspergillus flavus, Candida albicans. The Minimum Inhibitory Concentration from the growth of this bacteria and fung ranges from 12.5 mg/ml to 200 mg/ml. Results: Among the tested compounds, 4- Nitro, 2- methyl imidazole hetrocycle-pathic compound exerted potent in vitro antibacterial activity against Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis and Staphylococcus aureus while compounds (imidazole, benzimidazole hetrocycle-pathic compound) exhibited potent in vitro antifungal activity against Candida albicans, Aspergillus flavus and Aspergillus niger. Conclusion: Our results indicated that the designed derivatives (4- Nitro, 2- methyl imidazole, imidazole and benzimidazole hetrocycle-pathic compounds) showed effective antibacterial and antifungal activity mainly against a series of gram positive and gram negative bacterial strains. On the other hand, the tested compounds had an appropriate spectrum effect, because they showed antibacterial effect on both positive and negative strains.        }, Keywords = { Antibacterial Agents, Antifungal Agents, Azoles, Toxicity, Minimum Inhibitory Concentration}, volume = {8}, Number = {4}, pages = {1106-1115}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1680-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1680-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {dadakhani, sonya and pazhang, yaghub and Imani, Mehdi}, title = {The Anti-cancer effects of Celastrol on K562 cell line}, abstract ={  Background and Objective: The level of NF-κB factor expression (a transcriptional factor which increases the expression of inflammatory genes) is often increased in various human cancers. Therefore, NF-κB inhibitors such as Celastrol may prevent cancer development. The purpose of this study was to evaluate the anticancer effects of Celastrol on K562 cells proliferation. Materials and Methods: First, the K562 cells were cultured and cytotoxicity effects of celastrol were determined by MTT assay. Hoechst staining and DNA electrophoresis are used to check apoptosis. Data analysis was performed using SPSS, version 16 and ANOVA test (P<0.05) Results: Statistical analysis of MTT assay data showed that the growth of treated cells with different concentrations of the Celastrol significantly decreased (P<0.05) and inhibitory effect of Celastrol was time and concentration–dependent; so in higher concentrations (8 µM) and 72 hours, the maximum effect has occurred. The IC50 value of Celastrol was obtained 4 µM. Also, the results of Hoechst staining and DNA electrophoresis showed that Celastrol caused fragmentation of cell nucleus and DNA. Conclusion: Based on the results, Celastrol decreases cells viability (P<0.05) and induces apoptosis in K562 cells, its effect is time and dose-dependent. In conclusion, the agent may be applied as an anticancer drug for treatment of chronic myeloid leukemia.    }, Keywords = { Celastrol, K562 cell line, anticancer effects, leukemia}, volume = {8}, Number = {4}, pages = {1116-1126}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1681-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1681-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Vesali-akbarpoor, leila and SamavatiSharif, Mohammad Ali and Mirzayi, Sima and Piraki, Parivash}, title = {The Comparison between the Effect of Moderate & prolonged Swimming Training on Liver Tissue LXRα Gene Expression & Serum Cholesterol and Triglyceride levels in Male Wistar Rats}, abstract ={Background & Objectives: Liver X-Alpha Receiver is a family of large hormone receptors that play a role in cholesterol homeostasis. The purpose of the present study was to investigate the effect of ten weeks of moderate and long term endurance exercise on the expression of the LXRα gene and the evaluation of serum levels of cholesterol and triglyceride in male rats. Materials & Methods: 18 male Wistar rats were randomly divided in to three groups of six, control, moderate and long swimming, in the range of 275±25g. The training groups swam in 32 degrees water for ten weeks and 5 days a week. The moderate group swam for one hour and a long group each session for three hours. After the completion of the exercise, blood sampling from the underlying vena-cava and tissue from the rat liver was performed. Real-time PCR was used to measure the of LXRα gene expression by Ampliqon mixer and DNA synthesis kits. Differences were determined by ANOVA One-Way method and the groups were compared by Tukey follow-up test. Pearson correlation was used to determine the correlation between levels of expression of LXRα gene with cholesterol and triglyceride. SPSS software version20 was used at the significance level of p≤0.05. Results: The moderate exercise group showed a significant increase in LXRα gene and a significant reduction in cholesterol and triglyceride compared to both control and prolonged groups (p=0.001). Also, LXRα gene expression in this group had a negative and significant correlation with cholesterol (rs=-0.912) and triglyceride (rs=-0.862). Conclusion: It seems that the positive mechanism induced by moderate swelling by increasing the expression of the LXRα gene results in the release of cell lipid makers.    }, Keywords = {Endurance swimming training, LXRα gene, Cholesterol, Triglyceride}, volume = {8}, Number = {4}, pages = {1127-1135}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1583-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1583-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Esmaeilnejad, bijan and abtahifouroshani, meysam and Tehrani, Ali Asghar and esmaeiligovarchingaleh, hadi and moshrefzadeh, farshi}, title = {Effect of Fasciola hepatica on acetic acid induced ulcerative colitis in rat model}, abstract ={Background & Objective: Ulcerative colitis is one of the common causes of gastrointestinal tract diseases in developing countries. This disease causes disability, especially in young adults, and imposes many social and economic burdens on the community. The aim of this study was to investigate the effects Effect of Fasciola hepatica extract on acetic acid induced ulcerative colitis in rat model. Materials & Methods: Thirty adult male Wistar rats were divided into 3 equal groups. Colitis was induced by administration of 4% acetic acid. Control group just was administrated phosphate-bufferedsaline (S.C.) and the other groups were received Prednisolone (0.5 mg/kg) and Fasciola hepatica extract (100 mg/kg) for 10 consecutive days.Since then, the animals were euthanized and intestinal tissue and blood samples were taken. Macroscopic and microscopic features, inflammatory mediators as well as cytokines, TNF-α and IL-6 were assessed. Results: The amount of the myeloperoxidase production was the same between the two treatment groups. As compared to the control, both of the treatment groups produced lower amount of TNF-α and IL-6, however this reduction was more pronounced in Prednisolone treated animals than those of extract treated. There were no significant differences between Prednisolone andFasciola extract regarding gross damages and histopathological features. Conclusion: since preparation of Fasciola extract is easy and simple, it may be used as ameliorative agent for ulcerative colitis.    }, Keywords = {Ulcerative colitis, Acetic acid, Rat, Fasciola hepatica, inflammation}, volume = {8}, Number = {4}, pages = {1136-1145}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1584-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1584-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Noormohammadi, khatereh and BabaeiBalderlou, Farrin and AbtahiForoushani, Seyyed Meysam}, title = {The Effect of Inhibition of Dopamine D2 Receptors on Some of the Peripheral Blood Mononuclear Cells of the Rat under Food restriction}, abstract ={Background & Objective: In previous studies, the effects of food restriction on the changes in immune responses and brain dopamine content have been determined. On the other hand, it has been shown that immune cells, in addition to dopamine production, also have dopamine receptors. The purpose of this study was to evaluate the effect of inhibition of D2 dopamine receptors on several functions of monocytes of peripheral blood in rat under food restriction Materials & methods: In this experimental study, 36 male Wistar rats (weighing 200-250 gr) were allocated into six groups (n=6), including control groups, food restriction (25%), food restriction (50%), food restriction (75%), food restriction 75% and Sulpiride and rats treated with Sulpiride. Sulpiride was injected Intracerebroventricular at a concentration of 50 μg / rat on day 21 after the study initiation. At the end, the Rats were bled and peripheral blood mononuclear cells were isolated by ficoll gradient method. Results: Food restriction caused a significant decrease in the activity of monocyte cells of gradient of peripheral blood mononuclear cells like neutral red uptake test and respiratory burst (NBT reduction test) simultaneously with decreasing lymphocytes proliferation after stimulation with phytohemagglutinin. Administration of Sulpiride with a 75% Food restriction resulted in the improvement of these functions of monocyte cells of gradient of peripheral blood mononuclear cells as well as lymphocyte proliferation. Conclusion: Intracerebroventricular administration of dopamine D2 receptor antagonists (sulpiride) effectively inhibited the effects of a severe dietary restriction on the suppression of immunity system.    }, Keywords = {Dopamine, Peripheral blood mononuclear cells, Food restriction, Sulpiride, Rat}, volume = {8}, Number = {4}, pages = {1146-1156}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1626-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1626-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} } @article{ author = {Jafarzadeh, Gholamhasan and Shakeryan, Saeid and Farbood, Yaghoob and Ghanbarzadeh, Mohse}, title = {Effect of One Session of Resistance Exercises on Expression of BDNF Gene and TrkB Receptor in Alzheimer Model Male Wistar Rats}, abstract ={Background & Objective: This study is intended to evaluate the effect of One Session of resistance exercises on expression of BDNF gene and TrkB receptor in Alzheimer model male Wistar rats.  Materials & Methods: 32 mature male Wistar rats with mean weight of 230 to 280 grams were chosen and divided into Alzheimer and Sham groups. Rats in Sham group received normal saline while rats in Alzheimer group received STZ via intraventricular injection. These rats were then divided into the following four subgroups: 1. Resting Sham, 2. Exercising Sham, 3. Resting Alzheimer, and 4. Exercising Alzheimer. The two exercising rat subgroups, exercised 3 times a week for a period of 8 weeks. Exercise included lifting weight from the ladder. At the end of 8th week and 24 hours after last exercise session, the rats scarified by head separation. Hippocampus tissue was precisely extracted and samples were sent to laboratory for molecular and cellular tests. To determine the gene expression, RT-PCR method was used for analyzing the data and ANOVA was used. Results: The amount of BDNF, and TrkB between exercising rats and resting rats. These amounts were much higher in exercising Alzheimer rats group. Conclusion: It seems that eight weeks of resistance exercises increased expression of BDNF gene and TrkA an TrkB receptor in Alzheimer model Wistar rats.}, Keywords = {Alzheimer, BDNF, Gene Expression, TrkB Receptor, Resistance Exercises, Rat}, volume = {8}, Number = {4}, pages = {1167-1176}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1836-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1836-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2018} }