@article{ author = {Rostami, Maryam and RamezaniTehrani, Fahimeh and Simbar, Masoomeh and HosseinPanah, Farh}, title = {Relationship between Maternal Blood Vitamin D Levels and Pregnancy Outcomes: A Review Article}, abstract ={Background & Objective: Vitamin D deficiency is common during pregnancy and at delivery. Recent data suggest that vitamin D deficiency in mothers is associated with some adverse pregnancy outcomes. This study is designed to determine the relationship between the low maternal serum vitamin D levels and several pregnancy outcomes such as: pre-eclampsia, gestational diabetes, bacterial vaginosis and caesarean section. Material & Methods: This study is a review of the observational study (descriptive and analytic) articles published during 10 years (2000-2014) in English with full text which were indexed in PubMed, Scopus, Google Scholar, Iranmedex, Science Direct and and the SID databases. Results: A collection of 25 observational study from 168 articles which were related to 25-hydroxyvitamin D and adverse pregnancy outcomes were studied. The results indicated that the low levels of serum 25(OH)D in pregnant women is a risk factor for pre-eclampsia, bacterial vaginosis and cesarean section, but in relation to preeclapsia this indication is focuse on the second and the third trimester of pregnancy and there is no agreement about the risk for gestational diabetes and vitamin D deficiency among all the researches. Conclusion: Several pregnancy outcomes may be related to mothers’ serum 25(OH)D levels. However there is a significant need to perform more researches such as randomized clinical trials to investigate the effect of supplementation and screening on the maternal and neonatal outcomes.}, Keywords = {Vitamin D deficiency, Pre-eclampsia, Gestational Diabetes, Cesarean Section, Bacterial vaginosis}, volume = {6}, Number = {1}, pages = {1-18}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-848-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-848-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Jabbedari, Mohammad Mehdi and Karami, Jalal and SarkargarArdekani, Ali}, title = {The location analysis of the local pharmacies by the multi criteria decision-making AHP-Fuzzy method (case study IRAN-SHIRAZ)}, abstract ={Background & Objective: Due to the burden on citizens seeking access to the pharmacies, the optimal location of pharmacies, patients' access to medicines, and the pharmacies proportional distribution, are some significantly important factors to consider.. The main objective of this study was to evaluate the current distribution of pharmacies and to determine the best location for the establishment of new pharmacies in the study area. Materials & Methods: This study was a case study that was conducted in the city of Shiraz. This study collected data in the form of a map to locate the service centers, weighting the criteria and compare them with other test methods which were used to analyze the hierarchy in the geographical information system (GIS). The layers (maps) used  in this study were eight dual-layer maps, including 200 meters of daily pharmacies and 1000 meters of overnight pharmacies. In addition, the final weight was calculated for each layer by AHP-Fuzzy method. Results: 24 locations in the city were demonstrated, as the best places to establish a new pharmacy according to the research criteria layers. The incompatibility rate of 0.8 was calculated, which shows the weight of the layers located within the accepted range. The government hospitals and pharmacies layers are in the highest weight (23%) and the lowest weight (3%) respectively. Conclusion: By the combination layers of information on the final map, the locations were identified for the establishment of pharmacies. This study showed that the current location of pharmacies in the city of Shiraz do not match the standards and requirements.}, Keywords = {GIS, Location of pharmacies, Multicriteria decisionmaking, Weighting hierarchical analysis}, volume = {6}, Number = {1}, pages = {19-26}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1093-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1093-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {BakhshiGanje, Mohammad and Shekhha, Mohamad hassan and Farashahi, Ehsan and Mohammadzade, Mortez}, title = {Association of rs16953002 FTO Gene Polymorphism with Type 2 Diabetes and Obesity}, abstract ={Background & Objective: Genome-wide association studies (GWAS) has identified that FTO gene polymorphisms was associated with the risk of type 2 diabetes and obesity. However, the findings in subsequent studies were inconsistent in various populations. This study aimed to investigate the association of FTO rs16953002 with type 2 diabetes and obesity in Yazd, Iran. Methods & Materials: Unrelated study subject controls (n=200) and type 2 diabetes cases (T2DM) (n=200), were randomly selected from Yazd, Iran. Genotyping was done by the polymerase chain reaction-restriction fragment length polymorphism method. Age and gender, smoking, weight and body mass index (BMI) were analyzed using the analysis of χ2 test. Results: The rs16953002 A/G polymorphism of the FTO gene showed significant association with T2DM and obesity. The AG, GG genotypes were significantly higher in T2DM and obese subjects compared to the group subjects. Conclusions: The result of the present study indicate that the genetic variant in FTO may contribute to T2DM and obesity risk in Yazd population.}, Keywords = {FTO gene, Type 2 diabetes, Obesity, Polymorphism}, volume = {6}, Number = {1}, pages = {27-34}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-762-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-762-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Salehi, Maryam and Jafari, Mahvash and ASgari, Alireza and Rasouli, Jav}, title = {The Impact of N-acetyl Cysteine on Paraoxon-induced Oxidative Stress in Rat Liver and Kidney}, abstract ={Background & Objective: Paraoxon (POX) as one of the most toxic organophosphorus pesticides, is widely used in agriculture, which can reduce the antioxidant capacity of the cell. The aim of this study was to investigate the effect of N-acetyl cysteine (NAC) as an antioxidant against POX-induced oxidative stress in rat liver and kidney. Material & Methods: In the present experimental study, male Wistar rats were randomly divided into four groups; control (corn oil as POX solvent), POX (0.7 mg/kg), NAC (160 mg/kg), and NAC+POX. 24 hours after the intraperitoneal injection, animals were anesthetized and the liver and kidney tissues were quickly removed. Followed by the tissues hemogenation, superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and lactate dehydrogenase (LDH) activities, as well as glutathione (GSH) and malondialdehyde (MDA) levels were determined by the biochemical methods. The data were statistically analyzed using the analysis of variance (ANOVA) followed by the post hoc analysis using Tukey test. Results: POX increased SOD, CAT and GST activities and decreased GSH content in rat liver and kidney as compared to control. In addition, the increased MDA level and decreased LDH activity were observed in the liver. The administration of NAC inhibited changing of these parameters. Conclusion: The administration of NAC as antioxidant, ameliorates POX-induced oxidative stress in rat liver and kidney by scavenging the free radicals and GSH synthesis, but does not provide the complete protection.}, Keywords = {Paraoxon, N-acetyl cysteine, Oxidative stress, Liver, Kidney, Rat}, volume = {6}, Number = {1}, pages = {35-43}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-786-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-786-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Tafvizi, Farzaneh and Masomi, Masomeh}, title = {Association of Body Mass Index with Leptin and Other Hormonal Parameters in Women with Unexplained Infertility and Fertile Women}, abstract ={Background & objective: Obesity, as a major health concern in developed and developing countries, has many outcomes for people, among which is infertility in women. Therefore, this study was conducted to evaluate the correlation between the Body Mass Index (BMI) and the hormonal parameters in patients with unexplained infertility and normal subjects. Materials & Methods: This case-control study was conducted on 40 patients with unexplained infertility and 30 fertile women with inclusion criteria of Follicle-stimulating hormone (FSH)<10, BMI<30, Anti-Müllerian hormone (AMH)≥1, normal Antral follicle count (AFC), and under the age of 35 years, after obtaining the informed consents, in Valiasr Fertility Clinic of Imam khomeyni Hospital in 2013-2014. On the third day of menstruation, the hormonal profile (including LH, FSH, Thyroid stimulating hormone (TSH), Prolactin (PRL), and AMH was measured and then, 5 ml of blood was taken. In addition, the serum leptin was measured using the leptin ELISA kit, and the correlation between BMI and all the hormones was evaluated. Results: In all samples, the mean age, hormonal profile, and BMI had no statistically significant difference in the two groups. A direct and significant correlation was observed between BMI and leptin (p=0.003), and also between FSH and BMI (p=0.012) in the fertile women group. Conclusion: Given the presence of a correlation between leptin and BMI, and the effect of leptin on ovulation, it is recommended that the infertile women maintain the BMI in the normal range, exercise and put the physical activity in their daily life schedules.}, Keywords = {Body Mass Index (BMI), Leptin, Unexplained infertility}, volume = {6}, Number = {1}, pages = {44-51}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-934-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-934-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {PouraliSheshblouki, Gholamreza and Mardaneh, Jalal and Hosseinzadeh, Zahr}, title = {Klebsiella pneumoniae Infections in Hospitalized Patients: Characterization of Antibiotic Cross-resistance and Detection of Cefepime Susceptible-dose Dependent (SDD) Strains}, abstract ={Background & Objective: Klebsiella pneumonia (K. pneumoniae) is a Gram-negative enteric bacterium that causes nosocomial infections. The aims of the present study were to identify the Klebsiella pneumoniae infections in hospitalized patients: the characterization of cross-resistance and antibiotic resistance patterns in isolates and the detection of cefepime susceptible-dose dependent in strains. Materials & Methods: In the present study (during 2014-2015), samples were collected from patients hospitalized in Ghotbadden, Faghihi and Nemazee hospitals (Shiraz, Iran). The isolates were identified as K. pneumoniae, based on the biochemical tests embedded in the API-20E system. The susceptibility testing (disc diffusion) was performed according to the clinical and laboratory standards institute guidelines (CLSI 2014). The dDetection of cefepime susceptible-dose dependent (SDD) was performed. The detection of AmpC β-lactamases producing strains was done based on the cefoxitin and cefepime disk tests. Results: In this study 111 strains of Klebsiella pneumoniae were isolated. The less effective drug was ceftazidime (37.8% were sensitive). All SDD strains were susceptible to colistin and imipenem. Colistin (96.4%) and imipenem (88.3%) were the most effective antibiotics against isolates. Respectively, 41.4% and 35.1% isolates displayed resistance to gentamicin and amikacin. All colistin resistant isolates were imipenem sensitive. Conclusion: Carbapenem drugs are effective against Klebsieella pneumoniae infections. These results indicate that multidrug-resistant strains of K. pneumoniae are rising. The routine investigation and reporting of antibiotics resistance profile in patients presenting with Klebsiella infections is suggested.}, Keywords = {Hospitalized patients, Bacterial infection, Klebsiella pneumoniae, Antibiotic resistance pattern, Susceptible-dose dependent}, volume = {6}, Number = {1}, pages = {52-59}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-818-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-818-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Choobini, Neda and Azarpira, Negar and Mashayekhi, Mohamad Rez}, title = {Association of PNPLA3 Gene Polymorphism (rs738409) and Nonalcoholic Fatty Liver Disease in Southern Iranian Population}, abstract ={Background & Objective: Nonalcoholic fatty liver disease (NAFLD) is the spectrum of NAFLD ranges from simple steatosis to hepatocellular carcinoma. Genetic factors play an important role in developing the disease. Single nucleotide polymorphism (C> G) rs738409 of PNPLA3 gene increase liver fat content and metabolic disorders associated with liver damage. In this study, the relationship between this gene polymorphism with NAFLD and demographic and clinical characteristics were evaluated in the south Iranian population. Materials & Method: In this case-control study, 95 patients and 183 control subjects were studied. Blood samples from a cohort of Kavar were collected and normal blood donors from Shiraz blood transfusion center. The polymorphism genotypes were determined by PCR-RFLP. Results: Our results showed that there is significant association in genotype and sex parameter between patients and control subject (P-value(CG)=0.01, (SEX)< 0.005). Also we identified that there is no association between genotypes with demographic and clinical parameters. Conclusion: In the present study, G allele wasn’t a risk allele and CG genotype Carriers had a 2.63-fold (95% CI, 1.16 to 5.98, p-value = 0.01) increased risk of NAFLD. Age were identified as a risk factor for NAFLD in this study and by adjusting the variable age and three genotypes, it showed that by considering the age factor, the genotype didn't have a role in disease development. Also the no association between genotypes with demographic and clinical parameters was identified  }, Keywords = {Nonalcoholic fatty liver disease, PNPLA3, Polymorphism}, volume = {6}, Number = {1}, pages = {60-68}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-821-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-821-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Mobasheri, Farzaneh and Azizi, Hadi and Rastbaf, Fahimeh}, title = {The Epidemiological Pattern of Injuries among Children under 15 Years of Age in Fasa in 2013}, abstract ={Background & Objectives: Every year millions of children die due to injuries. Since children are vulnerable to injuries, appropriate measures must be taken to prevent injuries in this group. To achieve this goal, it is vital to recognize the epidemiological patterns of injuries. The purpose of this study is to determine the frequency and characteristics of child injuries in Fasa (Fars, Iran) so as to direct the local and national health authorities’ attention towards controlling and preventing child injuries. Materials & Methods: This cross-sectional study was conducted in 2013 on 1245 children between 0-14 years of age suffering from injuries who referred to Vali-Asr Hospital. The data were extracted from the national software injuries registry program. They were analyzed by Chi-square test, Fisher exact test, and Independent T-Test, using SPSS19 software. Results: This study showed that 1245 children were injured in 2013 (mean age of 6.5 ± 1.4 years). The majority of the injured were boys (62%) and two-year-old children (11.1%). Most of the injuries happened in urban areas (58.9%), in winter (33.8%), and at home (65.9%). Moreover, trauma (34.9%), falling (31.3%), and poisoning (16.1%) were more common in comparison to other injuries. Intentional and domestic injuries were significantly higher in girls (p = 0.039 and p < 0.001; respectively). Conclusion: According to the results, the priority of child injuries includes trauma, falling, and poisoning. Thus, these injuries should be considered in planning for the preventive measures of child injuries.}, Keywords = {Epidemiological pattern, Injuries, Children, Fasa}, volume = {6}, Number = {1}, pages = {69-78}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-847-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-847-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Davoudi, Ziba and Ghanbarzadeh, Mohsen and Shakeriyan, Saeid and Habbibi, Abdolhami}, title = {The Effect of Different Intensities of Acute Aerobic Exercise on Plasma Resistin Concentration and Insulin Resistance Index in Type 2 Diabetic Males}, abstract ={Background & Objectives: Today, the increase in diabetes all over the world and positive results of locomotor activity and exercise on factors related to this disease have been taken into consideration. The aim of this study is to investigate the effect of different intensities of acute aerobic exercise on plasma resistin levels in men with type II diabetes. Materials & Methods: The psent study is a quasi-experimental one. Ten subjects with Type II diabetes after at least 10 hours of fasting in a crossover design with three levels of 40%, 60%, and 80% VO2max ran on a treadmill for three sessions. Blood samples were measured before, immediately after, and 24 hours after any severity. Two-way analysis of variance with repeated measures and Pearson correlation test were used to analyze the data. Results: The findings showed that resistin levels, glucose, insulin, and insulin resistance index (HOMA-IR) immediately after and 24 hours after the three intensities of acute aerobic exercise were not significantly different, as compared to exercise trials before There is no substantial difference between resistin, glucose, insulin, and HOMA-IR immediately after and 24 hours after the acute aerobic exercise at different intensities (p>0.05). There is also no noticeable relationship between resistin, glucose Conclusion: It can be stated that acute exercise with different intensities does not affect resistin action in individuals with diabetes. These results may be due to the constant energy cost which is equivalent to 300 kcal per session, having no influence on the study variables.}, Keywords = {Diabetes, Resistin, Insulin resistance, Aerobic exercise}, volume = {6}, Number = {1}, pages = {79-86}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-861-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-861-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Ghoreishi, Atena Sadat and MohammadianShahrbabaki, Fahimeh and Toghroli, Navid and SheikhFathollahi, Mahmoud and Hajizadeh, Mohammad Reza and Khoshdel, Alireza and Mahmoodi, Mahdi}, title = {Investigating the Effect of Sesamum Indicum Consumption on Biochemichal Parameters in Type 2 Diabetic Patients}, abstract ={Background & Objectives: Diabetes is one of the most common diseases which is usually seen in modern societies. Due to the increased risk of the disease and its related complications, in addition to the harmless properties of sesame seeds, this research was aimed to study the effect of consuming sesame seeds on some biochemical factors in type 2 diabetic patients. Materials & Methods: This clinical trial has been carried out on 60 type 2 diabetic patients who were randomly divided into two groups of 30 sesame seeds consumers (case) and 30 non sesame seeds consumers (control).The case group was given 60 grams sesame seeds per day for 2 months and the control group did not receive any sesame seeds. The patient information was collected by questionnaire. The fasting blood serum of patients at the beginning and the end of the testing were collected and the biochemical factors of this study were measured in laboratory. For the data analysis, two independent samples t-test and paired t-test were used. Results: In this study, the mean ± SD (Standard Deviation) of the patients’ ages in the two groups, sesame seeds consumers (case) and non sesame seeds consumers (control), was 72/5 ± 40/52 and 11/6 ± 00/56 years, respectively. The results showed that fasting blood glucose, HbA1C, cholesterol, triglycerides, LDL (Low-density Lipoprotein), ALT (Alanine Transaminase) and ALP (Alkaline Phosphatase) were statistically decreased after the consumption of sesame seeds (P<0.05). Conclusion: According to the results, and by considering the sesame seeds harmless effects, it can be suggested that probably this plant is useful as a complementary therapy for treatment and prevention of diabetes disorders.}, Keywords = {Sesame seeds, Type 2 diabetes, Biochemical factors}, volume = {6}, Number = {1}, pages = {87-95}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-897-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-897-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {ZareMarzouni, Hadi and Akrami, Rosa and Shalilian, Minoo and Kalani, Navid and NooriAhmadAbadi, Mosayeb and Kooti, Wesam}, title = {Investigating the Prevalence, Determining the Effects of Immunologic Sensitization and Clinical Symptoms Related to Allergens Existing in Khuzestan Province}, abstract ={Background & Objective: Recently, the increased prevalence of allergic diseases and this particular disorder causes lots of problems for those suffering from the disorder. Allergens are attributed to elements, which can bring allergies to those who are prone to it. They motivate the immune system, and activate biogenetic materials, released from mastocytes. Therefore, this study was performed to assess the prevalence of allergens causing diseases in Khuzestan Province. Materials & Methods: This study was a cross-sectional study, which involved 150 patients affected by allergies in 2014. The tools applied for collecting the information consisted of a checklist containing two parts: the patients’ satisfaction form and the demographic clues. In addition, for diagnosing allergies in patient's skin, prick test was conducted. Statistical analysis was performed using SPSS13 software. Result: In this study, 88% were sensitive to allergens. From those diagnosed, 86 patients were diagnosed with allergic rhinitis, 22 patients with asthma, 13 patients with skin allergies, and 29 of those patients suffered from a mixture of skin allergies, nose allergies, and asthma. In patients, the most predominant symptoms resulted in patients were sneezing (76.6%), rhinorrhea (72%), and itching (68%) (p˂0.001). There was a significant association between the family background of allergies and allergy diseases (p=0.012). The Salsola kali plant showed an increased number of allergy symptoms outbreak s in this area. Conclusion: According to this study, the amount of allergens and the rate of clinical symptoms is very high among the inhabitants of this area, therefore, it is recommended to conduct more studies in order to decrease the allergic symptoms and to identify the antigenic function of allergens in different climates.}, Keywords = {Immunologic Sensitization, Allergens, Hypersensitivit, Skin test, Khuzestan}, volume = {6}, Number = {1}, pages = {96-105}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-895-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-895-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Karimian, Razieh and Karimian, Maryam and Hadipour, Mojtaba and Heyat, Fatemeh and Janbozorgi, Azam}, title = {The Prevalence of Children’s Postural Abnormalities and Its Association with Sport Activity}, abstract ={Background & Objective: The future of each society is based on its youth. In addition, the physical-mental health as well as the presence of healthy and capable people is a considerable requirement for all the societies. Thus, the aim of this research was to study the prevalence rate of children’s postural abnormalities and its relationship with sport activity. Materials & Method: 148 children (74 boys and 74 girls) were randomly selected. The research tools were plummet (guideline), matrix sheet, mirror box and the personal characteristics questionnaire. All the results were analyzed by using chi-square, freedman statistical method and the spearman correlation coefficient. Result: The results indicated that there is a significant difference between the two normal and abnormal height structure states (p≤0.01). Similarly, there was a significant negative difference between the number of skeletal abnormalities and the exercise activities in girls (r= 0.355) and boys (r= 0.369). In the case of abnormalities comparison between the sexes, the freedman test results indicated that there is a significant difference between the ranking of the boys’ skeletal abnormalities as compared to the girls, in a way that the dorsal lord sis and flat back were the highest and the lowest in the girls, respectively. Moreover, the results of the person correlation coefficient test indicated that there is only a positive significant relationship between the age of the girls and the number of skeletal abnormalities (p≤0.01, r=0.586). Conclusions: According to the results of this research, it can be stated that majority of the children at least have single skeletal abnormality, resulted from some factors such as heredity, daily wrong habits, and non standard equipments and facilities. In addition, not attending the related sports activities may increase the chance of these skeletal abnormalities.}, Keywords = {Postural abnormality, Exercise, Children, Lordosis}, volume = {6}, Number = {1}, pages = {106-112}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1092-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1092-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Maleki, Mahdye and Nassiri, Mohammad Reza and Tahmoorespour3, Mojtaba and Qazvini, Kiyarash}, title = {Cloning and Expression of Helicobacter Pylori CagA Gene Antigenic Regions in E. coli}, abstract ={Background & Objective: Helicobacter pylori, which has infected about 50 percent of the world population, is one of the most common gastrointestinal pathogens and the most prevalent cause of gastro-intestinal diseases, such as chronic gastric ulcers, gastric and duodenal ulcers, gastric cancer and lymphoma. The CagA Gene (cytotoxin-associated gene A) is a major virulence factor of this pathogenic bacterium. The aim of this study was to construct a recombinant vector carrying the antigenic regions of CagA gene and also to express this gene. Material & methods: The major epitopes of the CagA gene were identified based on bioinformatics. Through this procedure, a gene fragment of 996 bp was isolated by PCR, inserted into a pET32a vector using the enzymatic digestion and then transformed into the E. coli strain BL2I (DE3). Results: The results of sequencing represented the successful cloning of the CagA gene in the expression vector and introducing the accessibility to the antigen. The molecular weight of the produced recombinant protein was estimated 57 KD on an SDS-PAGE gel and the accuracy of the protein expression was verified. Conclusions: The results of this study proved the successful cloning of the epitope area. The recombinant protein can probably be introduced as a good candidate for the production of IgY from the chickenimmunized and the control of Helicobacter pylori infection in humans. It could also be possibly used for the design of diagnostic kits and vaccines for Helicobacter pylori}, Keywords = {Helicobacter pylori, CagA, Recombinant protein}, volume = {6}, Number = {1}, pages = {113-119}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-811-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-811-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Moradi, Mohammad and Norouzi, Amin and Taatimoghadam, Maji}, title = {Prevalence of bla-CTX-M, bla-SHV, and bla-TEM Genes and Comparison of Antibiotic Resistance Pattern in Extended-spectrum β-lactamase producing and non-producing groups of Klebsiella pneumoniae Isolated from Clinical Samples in Kerman Hospitals}, abstract ={Background & Objectives: Antibiotic resistance among pathogens bacteria are an important problem noted worldwide. Beta-lactamases that are produced by Enterobacteriaceae have been located mainly on plasmid. Treatment of these bacterial infections which produced β-lactamase are a major problem. Materials & Methods: 111 Klebsiella pneumoniae isolates were collected from hospitals in Kerman; therefore, antibiotic susceptibility test was performed by disk diffusion method. At first, detection of ESBLs were performed by phenotypic confirmatory test, and presence of bla-SHV, bla-TEM, and bla-CTX-M were detected by PCR. Results: Resistant to ampicillin (92.5%) was more than others antibiotics, and the imipenem (89%) was the most effective antibiotic against Klebsiella pneumoniae isolates. Additionally, the resistance to all antibiotics in ESBLs-producing Klebsiella pneumoniae was more than that of Non-ESBL Klebsiella pneumoniae. After the detection of bla-SHV, bla-TEM, and bla-CTX-M genes in Klebsiella pneumoniae by PCR, 56 (50.4%) isolates presented these genes. Conclusion: With regard to high prevalence of ESBLs genes and high level of antibiotic resistance in bacteria, detecting these genes can prevent the extension of antibiotic resistance through these bacteria.}, Keywords = {Klebsiella pneumonia, Antibiotic resistance, β- lactamase genes, PCR}, volume = {6}, Number = {1}, pages = {120-128}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-862-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-862-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Mirsane, Sayed Alireza and Mirsane, Sayed Mojtab}, title = {Complementary article: The effects of hydroalcoholic extract of dracocephalum kotschyi on blood glucose and lipid profile in diabetic rats}, abstract ={}, Keywords = {}, volume = {6}, Number = {1}, pages = {129-130}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1068-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1068-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Mohammadi, Jamshi}, title = {}, abstract ={}, Keywords = {}, volume = {6}, Number = {1}, pages = {131-132}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1149-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1149-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {}, title = {}, abstract ={}, Keywords = {}, volume = {6}, Number = {0}, pages = {0-0}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1194-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1194-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Hosseini, Maryam and Khoshnoud, Reza and Gholamzadeh, Saeed and Naseri, Gholamreza and Tarian, Tahereh and Malekpour, Abdorrasoul and Zarenejad, Mohamm}, title = {A Case Report of Suicide with Chloroquine Overdose and Review of Literature}, abstract ={Background & Objective: Suicide is considered as the tenth cause of death worldwide. There are several suicide reports consist in the use of certain unusual drugs, such as chloroquine. Case report: The cadaver of a 25-year-old single woman was found dead in her home and with suspect to using toxins or drugs was brought to Fars Province Forensic administration. She had history of psychiatric problems for which had referred to psychologist several times. Results: After the autopsy, there was no observation of pathologic lesions in her samples of liver, kidney, or heart. In bile samples, using Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC) methods, chloroquine was detected. In visceral and gut samples, chloroquine was found using TLC as +4 reactions and it was confirmed by HPLC and Gas Chromatography Mass Spectrometry (GC-MS). After examining all the aspects, eventually chloroquine overdose and its complications was determined as the cause of the death. Conclusion: Due to the high incidence of suicide in depressed patients and according to family and previous positive experience, preventive strategies based on the recognition and the treatment of depressed patients and also teaching the families to diagnose the illness in addition to the limitation of the free access to chloroquine and similar drugs is suggested to reduce overdose complications or suicide.}, Keywords = {Suicide, chloroquine, Depressed patients}, volume = {6}, Number = {2}, pages = {133-137}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-980-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-980-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Mazloum, Vahid and Sobhani, Vahi}, title = {The Effects of Resistance Exercises and Yoga on the Life Quality and the Muscle Strength of Patients with Knee Osteoarthritis: A Systemic Review of the Previous Literatures}, abstract ={Background & Objective: Knee osteoarthritis (OA) is a common musculoskeletal disorder in elderly population leading to pain, disability, muscle weakness, and decreasing quality of life (QOL). The objective of the present study was to investigate the effects of land-based exercise therapy intervention on QOL and muscle strength in patients with knee OA in previous researches. Materials & Methods: English literature associated with the effects of land-based therapeutic exercise methods were extracted from Medline, PubMed, CINAHL, Medlib, IrMedex, Magiran, Spring link, Scopus, Cochrane, and Science Direct databases since 2000 to 2016. After applying inclusion and exclusion criteria, 11 articles remained to be reviewed. Results: There were 1200 patients (Mean ± Standard deviation 64.5±4.7 years of old) with knee OA in those eleven studies. Generally, two interventions were administrated for such patients including exercise therapy and Yoga. The most common treatment outcome measurements involved Western Ontario and McMaster Universities Arthritis Index (WOMAC), Short Form 36 (SF-36), and strength. Conclusion: Although it was impossible to compare the results of the eleven studies due to inconsistency in outcome measurements and their units to introduce the most effective therapeutic intervention for improvement of QOL in individuals with knee OA, the positive effect of land-based exercise therapy on signs and symptoms of such disorder was confirmed.}, Keywords = {Knee Osteoarthritis, Land-based Exercise Therapy, Quality of Life, Function}, volume = {6}, Number = {2}, pages = {138-145}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-899-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-899-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Peyravi, Abdolnabi and Firouzi, Zahra and Meshkibaf, Mohammad Hass}, title = {The Protective Effects of Vitamins C and E on The Oxidative Stress Induced by Sodium Metabisulfite on The Kidney Tissue in Adult Rats}, abstract ={Background & Objective: Sodium metabisulfite which is used as a food preservative in the food industry, has adverse effects on body organs such as kidney and body grouth rate. In this research we have studied the protective effect of Vitamin C and E as antioxidants, on the kidney tissue damage after the consumption of Sodium metabisulfite. Materials & methods: Forty-eight Adult male Wistar rats of 150-200 grams were divided into 6 groups of 8 each. Rats in the experimental groups received Sodium metabisulfite (520 mg / kg body weight) by gavage feeding for 30 consecutive days. Also during this period, the experimental groups 2 and 3 received a daily dose of 100 mg / kg vitamins C and E, Respectively. The experimental group 4 received 50 mg / kg vitamin C plus 50 mg / kg of vitamin E by the same root. Control group received only normal diet and water. The placebo received vehicle (drug solvent) as well as normal diet and water. At the end of the exprimental period the body growth rate was measured between the groups. The histhopatological examination was performed on the kidney tissue sections. by light microscope Results: The results showed sodium metabisulfite in daily dietary could lead to the kidney tissue damage and reduced body weight in rats (p <0.05). However, vitamins C and E can reduce the kidney tissue damage and allow a normal growth weight (p <0.05). Conclusion: With this study we could conclude that the antioxidant effect of that vitamins C and E have a protective effect on renal damage induced by sodium metabisulfite consumption}, Keywords = {Sodium Metabisulfite, Vitamin E, Vitamin C, Kidney, Rats}, volume = {6}, Number = {2}, pages = {146-154}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-838-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-838-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Hejazi, Leila and Hosseini, Seyed Ebrahim}, title = {The Effect of Tribulus Terrestris Extract on Hepatic Complications Due to the Gelofen Consumption in Adult Female Rats}, abstract ={Background & Objective: Gelofen is one of the anti-inflammatory drugs which is used to relieve the pain and reduce the inflammations. This drug has side effects on body's tissues. This study aimed to investigate the effect of Tribulus terrestris (Tt) plant on hepatic transaminases due to the Gelofen consumption. Materials & Methods: In this experimental study 56 rats were divided into 7 groups of 8 rats, including control, sham and 4 experimental groups receiving Gelofen 400mg / kg, Tt extract 80 mg / kg, Tt extract 20mg / kg and Gelofen with 400mg / kg doses, Tt extract  40mg / kg  and Gelofen 400mg / kg, and Tt extract 80mg / kg  and Gelofen 400mg / kg. Gelofen was prescribed intraperitoneal and Tt was prescribed orally for 21 days. At the end of phlebotomizing the animals, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and Anaplastic lymphoma kinase (ALK) levels were measured, and the results were analyzed by ANOVA and Duncan tests. The significant difference in the data was considered P> 0/05. Results: The results showed that the transaminase concentration in the groups receiving Gelofen alone and with the Tt extract in doses of 20mg /kg and 40 mg /kg had a significant increase compared to the control group and the groups receiving Tt alone and Tt with the dose of 80mg/kg with Gelofen,had a significant decrease compared to the control and Gelofen alone groups (P> 0/05).  Conclusion: The results showed that the Tt extract led to prevent the negative effects of Gelofen on hepatic tissue in a dose-dependent manner and in result on the serum levels of liver transaminases.}, Keywords = {Gelofen, Tribulus terrestris, ALT, AST, ALK, Rat}, volume = {6}, Number = {2}, pages = {155-161}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-867-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-867-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Aryan, Reza and Ghasemi, Behzad and Beyzaei, Hamid and Najimi, Mohse}, title = {Evaluation of The Antibacterial Effects of The New Benzothiazole and Tetrahydropyrimidine Derivatives against Streptococcus Iniae, Edwardsiella Tarda and Aeromonas Hydrophila as Some Zoonotic Bacterial Pathogens}, abstract ={Background & Objective: The novel stronger antibacterial compounds such as the thiazole and pyrimidine derivatives are needed in order to remove the threat of bacterial antibiotic resistance in zoonotic aquatic bacterial pathogens. In this study, we evaluated the inhibitory effect of the new benzothiazole and tetrahydropyrimidine derivatives against three important zoonotic aquatic pathogens including Streptococcus iniae, Edwardsiella tarda and Aeromonas hydrophila. Material & Methods: Benzothiazole and tetrahydropyrimidine derivatives were synthesized and dissolved in DMSO with a concentration of 8129 μg/mL. Then, the disk diffusion and broth microdilution methods were applied to evaluate the antibacterial effects. Results were recorded as the minimum inhibitory concentration (MIC) and the growth inhibition zone diameter. Results: The study showed that the two tetrahydropyrimidine derivatives had no inhibition effects on all of the studied bacteria. Moreover, no inhibitory effect was observed from the three banzothiazole derivatives against A. hydrophila. However, the benzothiazole derivatives showed significant inhibitory effect against S. iniae and E. tarda with MIC of 256-1024 µg/mL and the growth inhibition zone diameter of 4.3±0.3-18.2±0.1 mm. Conclusion: The antibacterial effect of the new banzothiazole derivatives was confirmed on S. iniae and E. tarda pathogens for the first time.  }, Keywords = {Antibacterial, Zoonotic Bacteria, Benzothiazole and tetrahydropyrimidine}, volume = {6}, Number = {2}, pages = {162-168}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-951-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-951-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Sazegar, Hossein and Azarpira, Negar and Vahdati, Akbar and Karimi, Mohamad hossein and Soleimani, Masou}, title = {Differentiation of Mesenchymal Stem Cell toward the Insulin-like Cells with Lentivirus Vector Mir-375}, abstract ={Background & Objective: Type1 diabetes is characterized by autoimmune destruction of pancreatic β cells, leading to reduced insulin secretion. Differentiation of mesenchymal stem cells (MSCs) into β-like cells offers new ways of diabetes treatment. MSCs can be insulated from the human umbilical cord tissue and differentiate into insulin-producing cells. Material & Methods: Human umbilical cord–derived stem cells (hUDSCs) were attained after birth, selected by plastic adhesion, and considered by flow cytometric analysis. For the differentiation induction, miR-375 was lentivirally over expressed in hUDSCs. Results: Meaningful that microRNAs (miRNAs) are the key players in several stages of pancreatic Differentiation, we have presented a novel and cost-effective strategy in which the over-expression of miR-375 promotes the pancreatic differentiation in hUDSCs in the absence of any other stimulator. The major expressions of PDX1 and insulin were identified by quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR) (P <0.05). Conclusion: In conclusion, the Morphological assessment and the expression analysis of islet marker genes demonstrated that hUDSCs are able to differentiate into insulin-producing cells by transduction with lentiviral vector miR-375}, Keywords = {Diabetes, HUDSCs, MiR-375, Lentiviral Vector}, volume = {6}, Number = {2}, pages = {169-177}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-910-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-910-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Anbara, Hojat and Shahrooz, Rasoul and Malekinejad, Hasan and Saadati, Sadeq}, title = {Investigating the Antioxidant Properties of Royal Jelly and Vitamin C on Enzymes, Histomorphometric and Liver Cells Apoptosis in Mice Suffering Hemolytic Anemia}, abstract ={Background & Objective: Hemolytic anemia induced by phenylhydrazine (PHZ) as a hemolytic composition can change the function and structure of liver. Therefore, the present study attempts to evaluate the protective effects of vitamin C and royal jelly co-administration against the oxidative damages and liver apoptosis induced by hemolytic anemia in adult mice. Materials & Methods: 32 adult male mice were divided equally and randomly into four groups. The first group received normal saline with a dose of 0.1 ml, IP. The second group received a dose of vitamin C (250 kg/mg, IP) along with 100 kg/mg dose of royal jelly administered orally. The third group was administered with 6 mg/100 gr, IP phenylhydrazine in 48 hour intervals. Finally, the fourth group received vitamin C and royal jelly in the doses similar to the first three groups along with phenylhydrazine with the same doses of previous groups. After 35 days, the serum and testis samples were taken and were used for serum analysis and histochemical and histomorphometric studies. Results: Phenylhydrazine increased the level of serum concentration of aspartate transaminase, alkaline phosphatase, alanine transaminase, malondialdehyde and lactate dehydrogenase and decreased the superoxide dismutase along with the total antioxidant capacity and serum albumin. Moreover, phenylhydrazine increased the apoptosis, the number of kupffer cells and the diameter of hepatocytes. Prescribing the royal jelly with vitamin C improved the changes of abovementioned parameters significantly. Conclusion: Royal jelly with vitamin C is an antioxidant with the potential properties in preventing the oxidative damages and apoptosis induced by phenylhydrazine-induced hemolytic anemia in mouse liver.}, Keywords = {Anemia, Phenylhydrazine, Apoptosis, Liver}, volume = {6}, Number = {2}, pages = {178-187}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-929-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-929-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Kohan, Leila and Tabiee, Omi}, title = {The Role of rs2227956 HSPA1L Gene Polymorphism in Idiopathic Male Infertility}, abstract ={Background & objective: Male infertility is a multifactorial disease resulting from the interaction between the genetic and environmental factors. Despite the methodological advancements, the possible causes of infertility are still unknown for more than 25 percent of cases. Heat shock proteins (HSPs) are the molecular chaperones that are involved in different developmental stages of spermatogenesis. The current study was planned to investigate the role of HSPA1L rs2227956 gene polymorphism in the idiopathic infertility in males. Material & methods: This case control study was conducted on 342 subjects consisted of 143 patients with idiopathic male infertility and 199 control subjects. Followed by the DNA extraction from the peripheral blood, genotype determination was done by PCR-restriction fragment length polymorphism method. The logistic regression analysis was used to estimate the association between the polymorphism and male infertility. Results: A significant difference was observed in the genotype distributions between the cases and controls. The results showed that the individuals with TC and CC genotype had an increased risk of male infertility. In addition, there was a significant association between C allele and male infertility. Conclusion: HSPA1L rs2227956 polymorphism is associated with the idiopathic male infertility risk}, Keywords = {Male Infertility, Idiopathic, Polymorphism, HSP}, volume = {6}, Number = {2}, pages = {188-193}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-873-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-873-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Kazemi, Abdolreza and KalantariKhandani, Behjat and AshrafGanjoei, Afsoo}, title = {The Effect of 8 Weeks Endurance Training on Serum Levels of IL-10 and IL-8, and White Blood Cell Count in Women with Breast Cancer}, abstract ={Background & Objective: The regular exercise training is known as a preventive and adjuvant therapy in inflammatory diseases such as cancer. The aim of this study was to determine the effect of the aerobic training on IL-10 and IL-8 levels and the count of white blood cells (WBC) in women with breast cancer. Material & Methods: The statistical society of the present study included Kerman women with breast cancer. Forty patients with breast cancer were randomly divided into two groups: exercise (n = 20) and control (n = 20). The exercise group performed the endurance training for 8 weeks with the intensity between 40 to 55 percent of the target heart rate. Twenty four hours before the first session and 48 hours after the last session of the exercise protocol, blood samples were taken from both groups and then IL-10 and IL-8 levels in serum were measured by ELISA via a Boster kit. Results: The results of the present study showed that 8 weeks of the endurance exercise training did not significantly increase the IL-10 (P=0.113) serum level, but increased the WBC count (p=0.019) and decreased the serum level of IL-8 (p=0.03) significantly. Conclusion: According to the results of the present study, regular endurance training via decreasing the inflammatory factors can be considered as an effective factor along with other therapies in improving breast cancer.}, Keywords = {Endurance training, Breast cancer, IL-10, IL-8}, volume = {6}, Number = {2}, pages = {194-201}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-977-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-977-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Kashfi, Mansour and Nejat, Ghadir and Yazdankhah, Maryam and Hasanzadeh, Jafar and Rakhshani, Tayebeh and ManoochehriKhorammakani, Mahin and KhaniJeihooni, Ali}, title = {Investigating performance of rural family physicians in Fars province working as part of Family Physician Program}, abstract ={Background & Objective: Health family physician program is a complete system which eliminates the bewilderment of people and increases the satisfaction with health services as its most important results in medical care. The aim of this study was to evaluate the performance of family physicians and their strengths and weaknesses. Material & Methods: In this study, 52 family physicians were chosen via Random Stratified Sampling to participate in the study. A questionnaire titled “Performance of Family Physicians” with 5 domains of management, performance, contract guidelines, community involvement and results was used to collect data. Data were analyzed using SPSS-19 via t-test, ANOVA, Pearson correlation coefficient, and non-parametric tests. Results: Among the 52 studied family physicians, 56.9% were female and 43.1% were male. The lowest and the highest scores were obtained for the community involvement and results, respectively. Based on the results of this study, there were significant relationships among most of the domains. However, there was no significant correlation between the gender and different domains. Conclusion: In order to solve the problems of family physician program and improve the quality of services, more researches should be carried out soon to determine the types and causes of referring to the family physicians. Accordingly, appropriate interventions should be implemented to reduce the burden of visits and improve the quality of health services by guiding the society towards the prevention measures.}, Keywords = {Evaluation, Family physician, Performance}, volume = {6}, Number = {2}, pages = {202-209}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-966-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-966-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Ahmadi, Elahe and Abdollahi, Abbas and Najafipour, Sohrab and Meshkibaf, Mohamad Hasan and FasihiRamandi, Mehdi and Namdar, Najmeh and AbdollahiKheyrAbadi, Sara and Mousavi, Seyyed Mohammad and Samizadeh, Babak and Allahverdi, Ghader}, title = {Surveying the Effect of the Phenol Compounds on Antibacterial Activity of Herbal Extracts: In vitro Assessment of Herbal Extracts in Fasa-Fars Province}, abstract ={Background & Objectives: Due to increase in  bacterial drug resistance, discovering new antibacterial compounds is really important. The objective of this study is to evaluate the phenol compounds effect on antibacterial activity of herbal extracts of Fasa-Fars province in vitro. Materials & Methods: The antibacterial activity of 26 plants was studied by disk diffusion, well, and MIC methods in compare with 13 standard antibiotics against S. aureus and E. coli as control bacteria. Measurement of phenol compounds were performed by Seevers and Daly colorimetric methods using Folin-ciocalteu indicator. Results: Inhibition zone of bacterial growth  against S. aureus in well and disk methods were 32 and 22 mm in using Zataria multiflora, respectively .And there were 23 and 16 mm against E. coli in Zataria multiflora, respectively. Less effects and inhibition zones, less than 15mm on both strains, were seen in using  Saturina hortensis, Cinamomum zeylanicum, ­Artemisia absinthium, ­Urtica dioica, Carum carvi L. cyminum Cuminum, Achillea fragrantissimia, Marticaria chamomilla, Zingiber officinale, Origanum majorana, and Plantago psyllium. Most effective MIC results, 7.8 µg/ml, were related to the extracts of Zataria multiflora, Carum copticum L. Rosmarinus officinalis L., and Laurus nobilis L. Phenol compound amounts were approximately between 66.51±1.9 and 233.15±5.1 mg/gr extract in Zataria multiflora and Plantago psyllium, respectively. Conclusion: Results of antibacterial activity of extracts and relation with phenol compound amounts indicate the antibacterial effect of phenol compounds in herbal extracts.}, Keywords = {Herbal plants, Drug resistance, Antibacterial Effects, Phenol compounds}, volume = {6}, Number = {2}, pages = {210-220}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-852-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-852-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Shams, Nemat and Jaydari, Ami}, title = {Molecular Detection of Type 1 Fimbriae-Encoding Gene (fimH) in Uropathogenic Escherichia coli Strains Isolated from Outpatients with Urinary Tract Infection}, abstract ={Background & Objective: Urinary Tract Infection (UTI) is one of the most common infections in all age groups. The majority of these infections are caused by Uropathogenic Escherichia coli (UPEC) strains. Colonization, attachment to uroepithelium, and the ability of UPEC to cause symptomatic UTI is mediated by adherence factors, such as type 1 fimbriae (fimH). The objective of this study was to determine the frequency of type 1 fimbriae-encoding gene (fimH) among uropathogenic E. coli isolates from outpatients with UTI in Khorramabad. Materials & Methods: This laboratory study carried out on 100 uropathogenic E. coli collected in the years 2012 and 2013 from outpatients with UTI in Khorramabad. All bacterial isolates were identified by standard laboratory methods and the fimH gene presence was detected using the PCR method. Results: The fimH gene was amplified using specific primers and showed a band about 508 bp. The FimH gene was found in 26 isolates (26%) of the UPEC strains. Conclusion: Although results of this study showed the presence of type 1 fimbriae-encoding gene (fimH) among uropathogenic E. coli isolates from outpatients with UTI, the high prevalence of isolates that do not encode fimH (74%) require further investigation to clarify the role of the other potential virulence factors in the pathogenesis of these isolates.}, Keywords = {Urinary Tract Infections, Uropathogenic Escherichia coli, FimH}, volume = {6}, Number = {2}, pages = {221-226}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-933-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-933-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {AbtahiFroushani, Meysam and Nafisi, Saeed and EsmailiGourvarchinGaleh, Hadi and MansoriMotlagh, Bahman and ShahryariNor, Muhammad Seddigh}, title = {The Effects of Citrullus Colocynthis (L.) Hydroalcoholic Extract on the Function of Lymphocyte Proliferation and Innate Immune System Responses after Challenge with the REV1 Vaccine in Wistar Rats}, abstract ={Background & Objectives: The main objective of this study is to determine the possible effects of hydroalcoholic extract of Citrullus Colocynthis on the humoral and cellular immune responses in Wistar rats after challenge with REV-1 vaccine. Materials & Methods: The studied population included 20 male rats that were randomly divided into two equal groups and were immunized with Rev1 vaccine (0.1 ml Rev1+0.9 ml PBS (. Treatment group received hydroalcoholic extract of the C. colocynthis (50 mg/kg) orally every day from the beginning of the study and it continued for two weeks. Blood sampling was performed five days after the last injection. Moreover, 48 hours before blood sampling, Rev1 vaccine (0.1 ml Rev1+0.9 ml PBS (was injected into the left foot of rats. The levels of anti-Rev1 antibody and the specific cellular immune responses were measured by sero-agglutination test, footpad thickness, and griess colorimetric method, respectively. Lymphocyte proliferation, nitric oxide production, respiratory burst, and phagocytosis in splenocytes were determined by MTT test, Griess test, NBT assay, and slide test, respectively. Results: The levels of anti-Rev1 antibody, phagocytosis and Lymphocyte proliferation index in splenocytes were increased in treatment group compared to control group. Nevertheless, the levels of the cellular immunity (food pad thickness), NBT, and Nitric oxide in treatment group showed a significant decrease compared to control group (p<0/05). Conclusion: The hydroalcoholic extract of C. colocynthis may be used as a natural source for modulating the immune system}, Keywords = {Citrullus Colocynthis, Humoral immunity, Cellular immunity, Innate immunity}, volume = {6}, Number = {2}, pages = {227-234}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-944-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-944-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Rezaeyan, Abolhasan and Haddadi, Gholam Hassan and Hosseinzadeh, Massoo}, title = {Evaluating Superoxide Dismutase (SOD), Glutathione (GSH), Malondialdehyde (MDA) and the Histological Changes of the Lung Tissue after γ-Irradiation in Rats}, abstract ={Background & Objective: The lung is a radiosensitive organ and its damage is a dose-limiting factor in radiotherapy. Different side effects such as pneumonia and lung fibrosis are found in patients with thorax irradiation. The objective of the present study is to evaluate the effects of γ-irradiation on acute and chronic injuries of lung tissue in rats. Materials & Methods: 32 rats were divided into two groups. Control group consisted of 14 rats that underwent shame irradiation. In radiation group, 18 rats underwent γ-irradiation. The rats were exposed to γ-irradiation 18 Gy using a single fraction cobalt-60 unit. Eight rats in each group were sacrificed 24 hours after radiotherapy for determining Superoxide Dismutase (SOD), Glutathione (GSH), Malondialdehyde (MDA), and histopathological evaluations. Remained animals were sacrificed eight weeks after radiotherapy for histopathological evaluation. Results: Compared to control group, the level of SOD and GSH significantly decreased and MDA level significantly increased in radiation group 24 hours following irradiation, (p=0.001, p<0.001, p=0.001) respectively. Early histopathological results after 24 hours showed that radiation increases neutrophil, macrophage, and inflammation incidence compared to control group (p<0.05). Late histopathological evaluation after eight weeks revealed significant increase in factors including mast cells, pulmonary edema, vascular thickness, vascular damage, and also inflammation and fibrosis incidence in case group compared to radiation group  (p<0.05). Conclusion: Localized chest radiation with dose of 18 Gy induces changes in oxidative stress indices and histopathological lung tissue damage in short and long term.}, Keywords = {Superoxide Dismutase, Glutathione, Malondialdehyde, Histopathology, Gamma-radiation}, volume = {6}, Number = {2}, pages = {235-245}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1007-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1007-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Farzad, Babak and Rajabi, Hamid and Jameie, Seyed Behnamedin and Gharakhanlou, Reza and Hayat, Parisa and NasiriNejad, Farinaz and Damani, Sim}, title = {The Effects of Two-Week Swimming Training on Neuropathic Pain Induced by Chronic Constriction Injury and the Expression of GAD65 in Adult Male Rats}, abstract ={Background & Objective: Unknown mechanisms are involved in neuropathic pain. Among the non-pharmacological treatments, it seems that physical activity improves neuropathic pain. However, the possible reasons for the effectiveness of regular physical activity on neuropathic pain are unknown. Therefore, the present study was performed to determine the effects of two-week swimming training on the expression of GAD65 enzyme and P2X3 receptor in Chronic Constriction Injury (CCI) of the sciatic nerve. Materials & Methods: 40Wistar adult rats were divided into five groups randomly: 1) CCI neuropathic pain with swimming training (CCIST2); 2) CCI neuropathic pain without swimming training (CCI); 3) No CCI neuropathic pain with swimming training (ST2); No CCI neuropathic pain without swimming training (control group); 5) CCI sham surgery (Sham CCI). CCI and CCIST2 groups underwent peripheral nerve injury by four loose ligatures around sciatic nerve. Swimming program included two weeks with five sessions per week, and 30-60 min per session. The protein expressions of GAD65 enzyme and P2X3 receptor were evaluated by western blotting technique. Results: CCI surgery decreased the expression of GAD65, but two weeks swimming training increased expression of GAD65 comparing to CCI and Sham CCI groups (P≤0.001), but P2X3 receptor expression were not significantly different among groups in lumbar segment of rats (P>0.05). Conclusion: Totally, our findings showed that two-week swimming training improves neuropathic pain possibly through maintenance of inhibitory neurons and subsequently increased GAD65, which converts glutamate excitatory neurotransmitter to GABA inhibitory neurotransmitter.}, Keywords = {Neuropathic Pain, Swimming Training, GAD65, P2X3 Receptor}, volume = {6}, Number = {2}, pages = {246-254}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-947-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-947-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Khodadadi, Majid and Rahnama, Nader and Zamani, Jaber}, title = {Comparing the Effect of Balance Training with and Without Suit Therapy on the Balance and the Gait Pattern of Patients with Parkinsin\'s Disease}, abstract ={Background & Objective: Parkinson's Disease  is a progressive neurologic disorder affecting the central nervous system. The objective of this study is to investigate the effect of balance training with and without suit therapy on the balance and the gait pattern of patients with Parkinson's Disease. Material & Methods: participants with Parkinson's Disease were divided  into three groups of control, with suit therapy, and without suit therapy. The control group received only pharmacotherapy, while the groups with and without suit therapy received eigh weeks balance training in addition to pharmacotherapy. The patient's balance  and gait  were evaluted by Berg and Tinetti scales, respectively at the pretest and posttest of this study. Result: The result of the study showed significant difference in balance between the three groups (P<0/05). The groups with and without suit therapy were significantly more effective than control group (P<0/05), but between groups with and without suit therapy no significant difference was observed (P=0/076). The result of the study also revealed significant difference in gait between the three groups (P<0/05). The groups with and without suit therapy were significantly more effective than control group (P<0/05), and the group with suit therapy was significantly more effective than the group without suit therapy (P<0/05). Conclusion: To do balance traning is better than not to do it, and balance training with suit therapy is better than without suit therapy}, Keywords = {Balance traninig, Suit therapy, Balance, Gait, Parkinson's disease}, volume = {6}, Number = {2}, pages = {255-263}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1015-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1015-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Karimipour, Seyedeh Nasim and Tanomand, Asghar and Rostamnia, Sadegh}, title = {The Antibacterial Activity Evaluation of the Nanoparticles of Silver on Acinetobacter Baumannii}, abstract ={Background & Objective: Due to the high drug resistance in Acinetobacter baumannii, in this research, antibacterial properties of nano silver was evaluated for Acinetobacter baumannii. Materials & Methods: The nano silver with approximate diameter of 20 nanometer from Pishtazan Inc. Mashad, Iran and 5 nanometer from the Department of Chemistry in Maragheh University were prepared. Its concentration was determined by spectroscopy method in Tabriz Chemistry University.  Antimicrobial effects were determined by Mean Inhibitory Concentration (MIC) and Minimal Bacterial Concentration (MBC) by micro-broth-dilution method, disc diffusion and well diffusion methods. Anti-bacterial activity of nano-silver was tested for Acinetobacter baumannii NCTC12516 on 20 clinical strains (collected from Imam Reza Hospital in Tabriz). Results: The results showed the MIC and MBC of 20nm nanoparticles were 1250 ppm and 2500 ppm, respectively. On the other hand, the MIC and MBC of 5 nm nanoparticles were 156 ppm and 312 ppm, respectively. According to these findings, the MIC and MBC identified for clinical Acinetobacter baumannii strains under study along with the NCTC12516 strain did not show a significant difference. Yet the amount of inhibition for the 20nm nanoparticles in the density of 20000 ppm of clinical Acinetobacter baumannii and NCTC12516 strains was 11 millimeter with the disc diffusion method and 9.5 millimeter for the well diffusion method with the same concentration. The amount of inhibition of 5nm nanoparticles in the 250-ppm concentration with both disc diffusion and well diffusion methods was 9.5 millimeter. Conclusions: Acinetobacter baumannii is susceptible to nano-silver. Also the same MIC and MBC in multiple clinical strains suggests that there is not resistance to silver nanoparticles in Acinetobacter baumannii}, Keywords = {Acinetobacter baumannii, Silver nanoparticles, MIC, MBC}, volume = {6}, Number = {2}, pages = {264-270}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-868-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-868-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Movaseghi, Farzaneh and Sadeghi, Heydar and Hedayatpour, Nosratollah}, title = {Effect of Contraction Velocity on Selected Muscle Damage Indices Following Acute Eccentric Exercise-Induced Muscle Damage: A Review}, abstract ={Background & Objective: Eccentric muscle action is mechanically more efficient but employs a unique activation strategy which predisposes the muscle to damage. Type II muscle fibers are more susceptible than type I fibers to muscle damage; hence, velocity probably interferes with mechanical stress and thus may modulate muscle damage. The purpose of this review study was to investigate the effect of contraction velocity on selected muscle damage indices following acute eccentric exercise-induced muscle damage. Material & Method: Looking up related articles published in valid scientific databases such as PubMed, Springer, Elsevier, Science Direct, and SID with standard keywords and according to the research criteria, 16 studies (1980 to 2015) were selected. Results: Ten studies showed that high velocity eccentric exercise induced greater muscle damage. Five studies showed no differences between velocities, and a single study indicated a greater magnitude of muscle damage following slow eccentric exercise. Conclusion: Thus, greater magnitude of damage is induced by contractions performed at a higher velocity. However, considering differences during tension in the majority of studies, focusing on elbow flexor muscles and muscle damage profile variety in various muscle groups, and more animal and human studies in other muscular groups are necessary to confirm how the velocity of acute eccentric exercise would affect the muscle damage.}, Keywords = {Acute eccentric exercise induced muscle damage, Contraction velocity, Muscle damage indices}, volume = {6}, Number = {3}, pages = {271-282}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1153-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1153-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Sharifi, Nasibeh and FathNejadKazemi, Azita and Ghafari-Novin, Marefat}, title = {Infertility, Ovulation Induction, and Cancers Risk in Women: Review Article}, abstract ={Background & Objective: Using fertility treatment has increased markedly in recent decades. However, various concerns associated with the use of fertility drugs, such as increased risk of cancer, has developed. The objective of the present study is to overview studies in the field of infertility, ovulation induction, and its impact on cancer risks in women. Material & Methods: This study is a review of all articles published during the years 1995 and 2014 through databases SID, magiran, Medlib, pubmed, Scopus, Google Scholar, and Science Direct. Comprehensive search was done with the keywords of infertility, ovulation induction, the risk of cancer, gynecological cancer, clomiphene citrate, human chorionic gonadotropin, uterine cancer, ovarian cancer, and breast cancer for Persian articles and their English equivalents for English articles. After reviewing, a total of 79 articles were included in the study. Results: Various studies revealed the possibility of an increased risk of uterine, ovarian, and breast cancer and hormonal changes associated with ovulation stimulation. But these results are not conclusive because of small sample size, short follow-up periods, and vague information about the type and the way drug is consumed. Conclusion: Mainly women's cancer are associated with hormonal issues. Controversy over research results requisite further evaluation in order to investigate the impact of ovulation-inducing drugs on cancer risk which needs a well designed study.}, Keywords = {Infertility, Ovulation induction, Fertility drugs, Cancer, Women}, volume = {6}, Number = {3}, pages = {283-295}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-942-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-942-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Mollaei, Maryam and Rashki, Ahm}, title = {The Prevalence of Adhesive Surface Encoding Genes in Staphylococcus Aureus Isolated from Hospitalized Patients in Zabol-Iran by Multiplex PCR}, abstract ={Background & Objective: Staphylococcus aureus is one of the most frequently opportunistic pathogens isolated from nosocomial infections, responsible for severe infections such as bacteremia, endocarditis, and skin infections. Surface proteins such as fibrinogen and fibronectin-binding proteins are important factors in adhesion and invasion of S. aureus. Therefore, the objective of this study is to evaluate the presence of genes clfA, clfB, fnbA, and fnbB in isolates of S. aureus collected from clinical specimens of hospitalized patients in Hospitals of Zabol -Iran. Materials & Methods: In this cross-sectional study, 100 S. aureus isolates were collected from January to August 2013 from hospitalized patients at zabol-Iran. The isolates were confirmed by conventional biochemical tests. DNA of all isolates was extracted by boiling method. Multiplex PCR was used to identify the presence of virulence genes. The data were analyzed using Fisher's exact test. Results: The results of this study showed that 50% of isolates possess at least one of the studied genes. The frequency of genes encoding fibrinogen (clfA, clfB) and fibronectin (fnbA, fnbB) were 19%, 16% and 25%, 19% respectively. Conclusion: This study showed that the studied genes are found in small percentage of isolates. Further investigations on these genes are needed to clarify their role in the pathogenesis of S. aureus infections.}, Keywords = {Staphylococcus aureus, Fibrinogen, Fibronectins, Multiplex PCR}, volume = {6}, Number = {3}, pages = {296-302}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-992-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-992-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {RaayatJahromi, Alireza and TabatabaeiNaeini, Abutorab and Tanideh, Nader and Banan, Pegah}, title = {The Effect of Propofol Anesthesia on Cutaneous Wound Healing in Rats}, abstract ={Background & Objective: The present study surveys the effects of propofol on cutaneous wound healing in rats. Materials & Methods: 36 adult female Strague-Dawely rats were divided into three groups randomly; in group one propofol (60 mg/kg) and ketamine (40 mg/kg) combination; in group two ketamine (50 mg/kg), and in group three propofol (100 mg/kg) was injected intraperitoneally. Following routine surgical preparation, 1.5×1.5 cm wounds were created on the back of the rats. Wound size was evaluated daily and then the wound area was calculated by Digimizer software. Following euthanasia on day 21 after wounding, 1×1 cm skin samples were collected for histopathological evaluations and hydroxyproline content. Results: Wound size and hydroxyproline content showed no significant difference in propofol group compared to ketamine and propofol-ketamine groups. Fibroblast content and vascularity revealed no significant difference between groups. Inflammatory cell infiltration in ketamine group, collagen deposition in ketamine-propofol groups, and epithelial regeneration in propofol group were significantly higher in comparison with others. Conclusion: In conclusion, single use of propofol has no adverse effect on cutaneous wound healing in rats compared to ketamine, but evaluation of its positive effects on wound healing necessitates more detailed studies.}, Keywords = {Propofol anesthesia, Cutaneous wound, Rat}, volume = {6}, Number = {3}, pages = {303-310}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-962-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-962-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Mesri, Roghayeh and Esmaelizad, Majid and Ahmadzadeh, Mmozhghan and Mohammadi, Somayeh and Angaji, Seyed Abdolhamid and Yazdanpour, Zahra and MmirHaghghouyJali, Seyedeh Fahimeh}, title = {Cloning of linear T-cell epitops of EG95 antigen of Echinococcus granulosus into pGEX4t1 vector and expression analysis by SDS-PAGE}, abstract ={Background & Objectives: Echinococcus granulosus causes a common disease between humans and animals that called hydatid cyst or hydatidosis. The recombinant EG95 vaccine based on cloning of the 25 KD oncospheral antigen-Eg95- in pGEX stimulated significant humoral and cellular immunity in sheep, but regarding the effect of TH1cell immunity against this parasite and the influence of the linear T-cell epitopes in stimulating this immunity, only the coding sequence of linear T Cell epitopes of EG95 was cloned in pGEX and analyzed it's expression to optimize the qualification of this available vaccine in this study. Materials & Methods: The coding sequence of EG95 linear epitopes by IEDB software were predicted and synthesized. After PCR, the amplicon and pGEX4T1 were digested by xhoI restriction enzyme, the fragment was cloned into pGEX4T1 by heat shock method, and Positive colonies were selected by direct PCR with specific primers. The recombinant protein expression was evaluated in BL21 cells by 10% SDS-PAGE. Results: The coding sequence of EG95 linear T-cell epitopes was amplified by PCR and cloned into pGEX4T1 vector.  The recombinant plasmid was selected with Colony PCR and size difference between intact and recombinant purificated vectors. Recombinant protein expression with high significant concentration was recognized by SDS-PAGE. Conclusion: The EG95 linear T-cell epitopes coding sequence has been successfully cloned into pGEX4T1 vector and expressed into BL21 cells.}, Keywords = {EG95 antigen, Epitope vaccine, Hydatid cyst, T lymphocyte}, volume = {6}, Number = {3}, pages = {311-318}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1023-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1023-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Ahmadi, Elahe and Abdollahi, Abbas and FasihiRamandi, Mahdi and Namdar, Najmeh and Musavi, Mohammad and Samizadeh, Babak}, title = {Evaluation of Antibacterial Activity and Total Phenol Compounds of Punica granatum Hydro-Alcoholic Extract}, abstract ={Background & Objectives: Punica granatum is a non-productive form of a plant and is used for the treatment of diseases in traditional medicine. In this study, we evaluate the antibacterial activity and the total phenol compounds of Punica granatum. Materials & Methods: Disk and well diffusion methods and MIC were used to evaluate the antibacterial activity of hydro-alcoholic extract on S. aureus and E. coli compared to standard commercial antibiotic disks. Measurement of phenol compounds were performed by Seevers and Daly colorimetric methods (Folin-ciocalteu indicator). Results: 35 and 29 mm inhibition zones in S. aureus and 22 and 17 mm inhibition zones in E. coli were shown by disk and well diffusion method, respectively. Also, 7.8 mg/ml concentration of extract showed the MIC points for two bacteria. Phenol compound of extract was 233.15±5.1 mg/g of extraction. Conclusion: Antibacterial effect of Punica granatum compared to antibiotics indicates the strong activity against examined bacteria. Extensive antibacterial study of Punica granatum is suggested.}, Keywords = {Punica granatum, Antibacterial, Phenol compounds, Herbal extract}, volume = {6}, Number = {3}, pages = {319-325}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-853-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-853-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Jafarzadeh, Saeedeh and Mobasheri, Farzaneh and Bahramali, Ehs}, title = {Caregivers Awareness about the rules of Family Physician Program in Fasa in the Year 2014}, abstract ={Background & Objective: Caregivers awareness about family physician program is necessary to achieve its success and goals. This study was conducted to determine the caregivers’ awareness about family physician in urban areas of Fasa. Materials & Methods: This cross-sectional study was carried out on 411 patients referring to family physician clinics affiliated to Fasa University of Medical Sciences in winter 2014. The data were collected by a valid and reliable two-part questionnaire including demographic characteristics and 10 questions on people’s awareness about family physician program. The data were analyzed by using SPSS software version 19 by independent t-test, one-way ANOVA test, and chi square test. P-value less than 0.05 was considered significant. Results: 17.3%, 61.5%, and 21.2% of people reported appropriate intermediate, and poor awareness about the program, respectively. The mean awareness score  was 58/7 ± 14.5 (based on the score  100). But the mean score of the awareness about the rules and the referral system were higher compared to the awareness of how to get services in non-working hours from doctor, emergency services, hospitalization, and laboratory (9/15 ± 3/67 against 9/16 ± 2/50). Conclusion: The majority of participants in Fasa family physician program reported average knowledge about this program; therefore, it is essential to improve people knowledge in order to succeed in this program.}, Keywords = {Awareness, Caregivers, Family Physician, Fasa.}, volume = {6}, Number = {3}, pages = {326-333}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-952-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-952-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Bahram, Mohammad Ebrahim and Pourvaghar, Mohammad Jav}, title = {The Effect of 12 Weeks of High-Intensity Interval Training (HIIT) on Homocysteine and CRP Cardiovascular Risk Factors and Body Composition in Overweight Men}, abstract ={Background & Objective: High levels of homocysteine inflammatory markers and C-Reactive Protein (CRP) cause many complications, including atherosclerosis, venous thrombosis, and cardiovascular problems. The objective of the present study was to investigate the effect of 12-weekHigh Intensity Interval Training (HIIT) on homocysteine, CRP, and body composition in overweight men. Materials & Methods: In this quasi-experimental study, 20 students of Kashan University of Medical Sciences with a body mass index between 25 and 30 kg/m2, were purposefully selected and were randomly divided into experimental and control groups. The experimental group had practiced in the HIIT program with the intensity of up to 90 percent of maximum heart rate for 12 weeks. Before and after exercise, the amount of homocysteine, CRP, weight, body fat percentage, body mass index, and waist-to-hip ratio were calculated. The data were analyzed by using dependent and independent t-test at a significance level of P<0.05. Results: The results showed that12 weeks of HIIT had significant effects on reducing serum levels of homocysteine and HSCRP, body weight, body fat percentage, BMI, and WHR in the experimental group compared to the control group (P<0.05). Conclusion: It seems that 12 weeks of intense interval training as a non-invasive method can have a positive effect on reducing the amount of homocysteine, HS-CRP, and some anthropometric indexes of obesity and overweight.}, Keywords = {Homocysteine, HS-CRP, HIIT, Body Composition, Overweight}, volume = {6}, Number = {3}, pages = {334-342}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-948-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-948-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Movahedi, Mohammad Mehdi and Mehdizadeh, Alireza and Khalifeh, Behnam and Amani, Samad and Taeb, Shahram and Mostaghimi, Hesamoddi}, title = {Evaluation of Radiation Exposure of Urology Surgeons and Radiology Personnel during Fluoroscopy Guided Surgeries at Shahid Faghihi Hospital in Shiraz}, abstract ={Background & Objective: Long term exposure to ionizing radiation and its complication have always been concerned by medical physicists. Some previous studies have shown that radiation dose to urology surgeons and involved personnel during mini C-arm fluoroscopy guided surgeries exceeds the secure threshold in some cases. In this study, we intend to evaluate the radiation exposure of urology surgeons and radiology personnel and compare these results to the standard thresholds. Material & Methods: Four urology surgeons, eight residents and eight radiology personnel underwent radiation dosimetry on five different organs during three months. Mentioned surgeons used mini C-arm fluoroscopy and TLD-100s were used for dosimetry.     Results: Calculated mean for accumulative radiation exposure for Urology surgeons, residents and radiology personnel are 174, 198 and 184 milirem, respectively. The absorbed dose for different organs were calculated in descending order at dominant hand, thyroid, subdominant hand, Gonads and Chest wall.  Conclusion: According to the results, radiation exposure during fluoroscopy guided surgeries has not exceeded the standard threshold defined by National Council on Radiation Protection and Measurement (NCRP).   }, Keywords = {Urology surgeons, Radiology personnel, Dosimetry, C-arm Fluoroscopy}, volume = {6}, Number = {3}, pages = {343-348}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-836-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-836-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Khoshnam, Esmaeil and Jaafari, Marzyeh and Bahaoddini, Amin and Owjfard, Maryam}, title = {The Effect of Prolonged Exposure to Extremely Low Frequency Electromagnetic Fields on the β2 Adrenergic System Activity in the Small Intestine of Male Rats}, abstract ={Background & Objective: Electromagnetic waves with the frequencies of 0–300 Hz and the intensity of 0.1–100 millitesla can affect several cellular activities. Therefore, the purpose of this study is to investigate the effect of prolonged exposure to extremely low frequency electromagnetic fields (ELF) on the adrenergic system in the small intestine. Materials & Methods: 21 adult male rats were divided into three groups. The first group was experimental group which exposed to ELF (50Hz, 1mT) for 75 days in powered on solenoid. The second group was sham group which was kept in similar conditions as the first group but in powered off solenoid. The third group or the control group was kept in animal house condition. The isolated strips of the colon were inserted into organ bath and were linked to power lab A to D system force transducer and their mechanical activity were recorded in response to different doses of Isoproterenol (10-4 M and10-5 M). The data were analyzed using one-way ANOVA test. Result: The results showed a significant increase (p≤0.05) of ileum basal contractions in experimental group compared to control and sham groups. While the relaxation changes of ileum in response to the different doses of isoproterenol (β2 adrenergic receptors agonist) in the experimental group compared to the sham and control groups was not statistically significant. Conclusion: It can be concluded that prolonged exposure to extremely low frequency electromagnetic fields may not affect β2 -adrenergic receptors activity.}, Keywords = {Electromagnetic field, β2 -adrenergic, Ileum}, volume = {6}, Number = {3}, pages = {349-355}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-924-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-924-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Ghahraman, Minoo and Askari, Mohamad Hasan and Dehghani, Azam}, title = {Comparison of Effects of Isoflurane and Propofol on Body Temperature Changes during Knee Arthroscopy Surgery}, abstract ={Background & Objective: Anesthesiologists are often facing patients who suffer from hypothermia during anesthesia and shivering after surgery. This study compared the effects of anesthesia with isoflurane and propofol on the intensity of body temperature reduction during anesthesia in knee arthroscopy surgery. Material & Methods: The study was done as a double blinded randomized clinical trial. From 40 patients who underwent knee arthroscopy surgery, 20 subjects were placed in isoflurane group and 20 in the propofol group. Changes in surface and core body temperature, hemodynamic, and shivering after anesthesia in different times were evaluated. The central body temperature for each patient was measured through the tympanic membrane, and the surface temperature of the body was measured through the axillary. SPSS was used for statistical analysis. Data were compared via Student’s t-test analysis. Changes between time points were examined using repeated measurement ANOVA. Comparison of qualitative variables was done by Chi-square test between groups. Results: Core body temperature in propofol group significantly increased compared with the isoflurane group during anesthesia and after anesthesia (P<0.05). But Changes in body surface temperature showed no significant difference (P>0.05), and surface body temperature in propofol group significantly increased in the isoflurane group during and after the anesthesia (P<0.05). Chill rate was not significantly different between the two groups except in extubation and recovery (P>0.05); in the propofol group, the chill rate increased more significantly than the isoflurane group. Furthermore, changes in systolic blood pressure and diastolic blood pressure showed no significant difference between the two groups at different times during and after the anesthesia (P>0.05). Conclusion: It seems that propofol is better than isoflurane in controlling body temperature during and after anesthesia and shivering post-surgery.}, Keywords = {Isoflurane, Propofol, Knee Arthroscopy Surgery, Body Temperature, Shivering}, volume = {6}, Number = {3}, pages = {356-364}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-291-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-291-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {AalaeiDiman, Shahin and Mohamadzade, Mehdi and Pazhang, Yaghub}, title = {Evaluation of Antitumor Activity of Valproic Acid on K562 cells: In Vitro}, abstract ={Backgrond & Objective: This study was performed to evaluate the antitumor effects of valproic acid (VPA) drug on K562 cells in vitro. Material & Methods: For this purpose, K562 cells were cultured and treated with different doses of valproic acid, and its antitumor properties were measured by MTT assay 24, 48, and 72 hours after treatment. Then, DNA electrophoresis and staining with Hoechst were used to analyze apoptotic cells in vitro. Results: The antitumor effects of valproic acid increased in a dose- and time- dependent manner. Also, the apoptotic effects of the drug were approved by electrophoresis. The maximum inhibitory effect was observed in higher concentration and after 72 hours treatment. IC50 was calculated 80Mµ by Compusyn software. The results showed that valproic acid was an efficient drug for inhibiting K562 cells, and its antitumoric effect would warrant further. Conclusion: Accordingly, valproic acid is suitable for inhibiting K562 cells, and these antitumoric properties would warrant further studies on the clinical application of valproic acid. Therefore, this drug might be effective for the prevention and treatment of chronic myeloid leukemia.}, Keywords = {Valproic Acid, In Vitro, Antitumoric Properties, K562 Cell Line, Apoptosis}, volume = {6}, Number = {3}, pages = {365-372}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-814-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-814-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Kazemi, Abdolreza and Kermani, Jamileh and Rahmati, Masou}, title = {The Chronic Effect of Endurance Training On Jip3 Gene Expression in Soleus Muscle of Male Wistar Rats}, abstract ={Background & Objective: Adaptations in neuromuscular system are due to changes in the expression and activity of proteins and its transmitters. JIP 3 protein plays a role in the regulation of structure and function of neurons as well as the establishment of multi-nuclear muscle cells. Considering the effects of exercise on neuromuscular system, the present study aimed to investigate the effects of 6 weeks of endurance training on JIP3 gene expression in soleus muscle of male Wistar rats. Material & Methods: Ten Wistar male rats were randomly assigned in two groups: trained and control. Endurance training protocol was performed for 6 weeks. Forty eight hours after final training session, the rats were dissected, and soleus muscle was removed. JIP3 gene expression was performed through Real Time PCR method. Results: Endurance training increased JIP3 gene expression significantly compared to the control group. Conclusion: It appears that a period of endurance training increases intramuscular gene expression of JIP3 through this can lead to a better consistency and functionality in the muscles.}, Keywords = {Gene Expression, Endurance Training, JIP3}, volume = {6}, Number = {3}, pages = {373-379}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-864-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-864-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Bamdad, Kourosh and RahimiGharemirshamlu, Fatemeh and Naeimi, Sirous}, title = {Bioinformatic Analysis of Deleterious Non-Synonymous Single Nucleotide Polymorphisms (nsSNPs) in the Coding Regions of Human Prion Protein Gene (PRNP)}, abstract ={Background & Objective: Single nucleotide polymorphisms are the cause of genetic variation to living organisms. Single nucleotide polymorphisms alter residues in the protein sequence. In this investigation, the relationship between prion protein gene polymorphisms and its relevance to pathogenicity was studied. Material & Method: Amino acid sequence of the main isoform from the human prion protein gene (PRNP) was extracted from UniProt database and evaluated by FoldAmyloid and AmylPred servers. All non-synonymous single nucleotide polymorphisms (nsSNPs) from SNP database (dbSNP) were further analyzed by bioinformatics servers including SIFT, PolyPhen-2, I-Mutant-3.0, PANTHER, SNPs & GO, PHD-SNP, Meta-SNP, and MutPred to determine the most damaging nsSNPs. Results: The results of the first structure analyses by FoldAmyloid and AmylPerd servers implied that regions including 5-15, 174-178, 180-184, 211-217, and 240-252 were the most sensitive parts of the protein sequence to amyloidosis. Screening all nsSNPs of the main protein isoform using bioinformatic servers revealed that substitution of Aspartic acid with Valine at position 178 (ID code: rs11538766) was the most deleterious nsSNP in the protein structure. Conclusion:  Substitution of the Aspartic acid with Valine at position 178 (D178V) was the most pathogenic mutation in the human prion protein gene. Analyses from the MutPred server also showed that beta-sheets’ increment in the secondary structure was the main reason behind the molecular mechanism of the prion protein aggregation.}, Keywords = {Non-Synonymous Single Nucleotide Polymorphism, Human Prion Protein, D178V}, volume = {6}, Number = {3}, pages = {380-388}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1088-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1088-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Hoseini, Seyed Ali and Zar, Abdosaleh and Hoseini, Seyed Ali and Hassanpour, Ghobad and Kheirdeh, Maryam and Noura, Mehdi}, title = {Effect of Eight Weeks Forced Swimming Training with Methadone Supplementation on Aspartate Aminotransferase, Alanine Aminotransferase, and Alkaline Phosphatase of Rats}, abstract ={Background & Objective: Narcotics abuse can induce liver disorders; nevertheless, exercises improve liver disorders. The present research aimed to review the effect of eight weeks forced swimming training with methadone supplementation on liver enzymes of rats. Material & Method: In this experimental research, 48 rats were selected, and after one week adaptation to lab environment, they were randomly divided into four groups of 12 rats including (1) forced swimming training, (2) methadone supplementation, (3) forced swimming training with methadone supplementation, and (4) control. Groups 2 and 3 used 2 mg/kg methadone daily for 8 weeks. Also, groups 1 and 3 swam for 8 weeks, three sessions per week and each session for 30 minutes. For statistical analysis of data, one way ANOVA and Tukey post hoc tests were used (α≤0.05). Results: Findings showed that forced swimming training, methadone supplementation, and forced swimming training with methadone supplementation had no significant effect on AST (P=0.90) and ALT (P=0.99) enzymes; forced swimming training had significant effect on increase of ALP (P=0.001); also, forced swimming training, compared with methadone supplementation and combination of forced swimming training with methadone supplementation, had significant effect on increase of ALP (P=0.001). Conclusion: Accordingly, 8 weeks of forced swimming training with methadone has possibly no significant effect on liver enzymes.}, Keywords = {Forced Swimming Training, Aspartate Aminotransferase, Alanine aminotransferase, Alkaline Phosphatase, Methadone}, volume = {6}, Number = {3}, pages = {389-398}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1043-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1043-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Mojtahedin, Ali}, title = {Investigation of Peripheral Effects of Citrus Limon Essential Oil on Somatic Pain in Male Wistar Rats: Role of Histaminergic System}, abstract ={Background & Objective: One of the plants used in traditional medicine is lemon which has analgesic effect. However, little research has been performed on the analgesic effect of lemon and mechanisms of action with an emphasis on neurotransmitters systems. Therefore, the present study set to investigate the peripheral effects of lemon essential oil on somatic pain using formalin test with an emphasis on histaminergic system in male Wistar rats. Materiala & Methods: Sixty male rats weighing approximately 200-250g and aged 14-16 wk were divided into 10 groups: sham (Salin + Formalin 1% intraplantar), three treatment groups with lemon essential oil (EO) (12.5, 25 and 50 mg/kg), three treatment groups with Chlorpheniramine (5, 10 and 20 mg/kg), 1 treatment group with Histamine (10 mg/kg), 1 pretreatment group with Chlorpheniramine (20 mg/kg) + EO (50mg/kg), and 1 pretreatment group with Histamine (10 mg/kg) + EO (50 mg/kg). Formalin test was used to assess somatic pain. Data analysis was performed using one-way ANOVA. Results:  Intraperitoneal injection of lemon essential oil reduced the pain response induced by formalin in both phases (P<0.05). Pretreatment with chlorpheniramine and lemon essential oil enhanced the analgesic response in both phases (P<0.05). Conclusion: Lemon essential oil had analgesic effects, probably caused by the histaminergic system.}, Keywords = {Citrus Limon L., Somatic Pain, Histaminergic System, Formalin Test}, volume = {6}, Number = {3}, pages = {399-408}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1073-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1073-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Soltanian, Mitra and Nikouee, Farhoo}, title = {The Relationship between Duration of Breastfeeding and Mother’s BMI}, abstract ={Background & Objective: Breast feeding is the only food created exclusively for human, and its ingredients are according to the needs of the infant's body and it provides the most appropriate growth conditions. Mother’s BMI is one of the most important factors in duration of lactation. Few studies have yielded conflicting results in this field. The present inquiry set to determine the relation between maternal BMI before pregnancy and duration of breast feeding. Material & Methods: A retrospective cohort study was conducted. The samples consisted of 243 mothers who had 2-4 years old children and were visited in Shiraz urban health centers for their growth monitoring. Stratified sampling method was applied, and the data were collected through questionnaires on their medical records. Results: There was a statistical significant relationship between BMI and breast feeding duration. There was not any statistical significant relationship between duration of breast feeding and mother and her husband’s education, mother's occupation, mother's age, type of delivery, and child gender. Conclusion:  Considering the relationship between short duration of breast feeding and maternal obesity, health education should be made for balancing mother's weight and contribute to the health of mothers and their children. Further research is recommended to confirm the results.}, Keywords = {Body Mass Index, Duration of Breast Feeding, Lactating Women, Shiraz}, volume = {6}, Number = {3}, pages = {409-415}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1003-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1003-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Sasani, Mohammad Rez}, title = {The Orbital Hemangiopericytoma: The Number of Reported Cases and a Possible Risk Factor}, abstract ={I read, with interest, the article entitled “The Orbital and Sinonasal Hemangiopericytoma, 2 Case Reports” by Maryam Maghbol et al. published in this journal. I would like to draw attention to the number of reported orbital hemangiopericytomas and a possible risk factor for this tumor. Accordingly, in the discussion and conclusion section, the authors claimed that thirty-three cases of orbital hemangiopericytomas already has been published. However, with regard to the literature, at least 50 cases of this tumor already has been published. Moreover, the authors affirm that no specific risk factor already has been recognized for this tumor. In a study stated that previous trauma has a possible role in the origin of hemangiopericytomas.}, Keywords = {Orbital Neoplasms, Hemangiopericytoma, Risk Factors.}, volume = {6}, Number = {3}, pages = {416-416}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1060-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1060-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Nadri, Samad and Saburi, Ehsan and Nadri, Parisa and Brati, Ghasem}, title = {Isolation Methods of Mesenchymal Stem Cells in Mice; a Systematic Review}, abstract ={Bone marrow (BM) has been regarded as the primarily source of hematopoietic stem cells for many years. Recent researches have shown BM contains not only hematopoietic but also heterogeneous non-hematopoietic stem cells. Till now, similar or overlapping isolation strategies of primitive non-hematopoietic stem cells in BM were named by different investigators and hence were assigned different names (e.g. mesenchymal stem cells, or multipotent adult progenitor cells) from alternative sources and different animal species. The differentiation potential of these cells to mesenchymal lineages has generated a great deal of interest in regenerative medicine and tissue engineering. Murine Mesenchymal stem cells (mMSCs) are appropriate models for preclinical investigations on human cell therapies. The isolation of BM-derived mMSCs is discussed as an unresolved issue in mesenchymal stem cells researches. The objective of the present review is the description of multiple methodological approaches to isolate mMSCs.}, Keywords = {Murine mesenchymal stem cells, Bone Marrow, Isolation}, volume = {6}, Number = {4}, pages = {417-425}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1096-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1096-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Shojaeifard, Manzarbanoo and Malekzadeh, Samira and Shariati, Mehr}, title = {A Review of the Teratogenic Factors Effect on Embryo}, abstract ={Background & Objectives: Teratology is a branch of embryology science that studies causes, mechanisms and abnormal pattern development. Embryo growth traumatic factors during pregnancy are called teratogens that some teratogens pass the placental barrier and cause adverse effect during development stages and malformation, however a drug may improve general health of the mother, but it might be poisonous for embryo and cause diverse malformation. Since study of embryo health and risk factor in this stage is important, the aim of this review article was the investigation of some types of teratosgens (such as radiation, infectious agents, heat disorders, maternal conditions) and particularly the effect of teratogenic drugs on embryo including some legal drugs (such as acetaminophen, thalidomide, acyclovir, sedatives and anticonvulsants) and illegal drugs (such as nicotine, alcohol, cocaine and marijuana). Conclusion: In general, teratogens depending on the type and duration of exposure in pregnancyperiod, adversely affect embryo and cause various disorders. A better understanding of these teratogens can contribute to prevent these defects, since many other drugs with similar effects and lower teratogenicity can be used to improve mothers’ health.}, Keywords = {Teratology, Teratogen, Teratogenic Drugs, Embryo Development}, volume = {6}, Number = {4}, pages = {426-439}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1156-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1156-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Azadbakht, Mehri and Ramezani, Mina and Ebrahimi, Soheila and Moradi, Hanieh}, title = {The Study of Retinoic Acid Effects on Testicular Development of NMRI Mice}, abstract ={Background & Objectives: Retinoids are important molecules that regulate crucial processes of development in all vertebrates. In this article, we study the effect of retinoic acid on testis development. Materials & Methods: A group of newborn NMRI mice was chosen to receive intraperitoneally injections of 25 mg/Kg.b.w of retinoic acid and the control group had no injection. After 21 days, the male animals were isolated and sacrificed in 60 days postpartum, and testes were removed from their bodies. Apparent characteristics of the testes of seven mice from each group were observed. In the light microscopic study, these epithelial cells of the tubules were counted and the diameter of seminiferous tubules were measured with statistical T-test analysis and were compared with control group. Results: The findings showed that the weight of the testes in animals that were affected by retinoic acid did not change significantly compared to control group. In germ epithelium of testis, the thickness of the epithelium and the number of the spermatogonia, round and elongated spermatids has decreased significantly compared to the control group (P<0.05). Conclusion: According to the findings of this article, using the retinoic acid after birth has influence on testis development and its seminiferous tubules epithelium. Retinoic acid has an adverse effect on the cell divisions in seminiferous epithelium, therefore the number of affected germ cells decrease. Therefore, the use of vitamin A and its synthetic derivatives like retinoic acid for pregnant women should be done with caution.}, Keywords = {Retinoic acid, Development, Testis, Mouse}, volume = {6}, Number = {4}, pages = {440-446}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1034-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1034-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {HaghNegahdar, Abdolaziz and Lohrasb, Sadaf and Khojastepour, Leil}, title = {Knowledge, Attitude and Performance of Shiraz General Dentists about Infection Control Principles during Preparing Intraoral Radiographies}, abstract ={Background & Objective: Infection control in dental centers is affected by the persons’ attitude and knowledge about mechanisms of infection transmission. This study was designed to evaluate the knowledge and the attitude of Shiraz dentists about infection control during intraoral radiographies preparation. Materials & Methods: In this cross-sectional, and analytical research, the attitude and the knowledge of 45 male and 25 female, randomly selected dentists, were obtained through completion of a researcher- planed questioner which its validity and reliability had been confirmed. Data were analyzed using Cronbach`s alpha, one-way ANOVA, student’s t-test, and Pearson’s correlation coefficient in SPSS (V.21). Results: The average of the dentists’ age was 40.59±10.72 and their average occupational experience was 13.49±9.75 years. The mean score obtained for knowledge about infection control during intraoral radiographic procedures was less than fifty percent of total obtainable score, and was assessed as weak knowledge. There was no significant difference in the level of knowledge between studied male and female dentists (P>0.05). In addition, no significant relationship was detected between level, age/experience, and the university of education (P>0.05). The attitude of the dentists about infection control during intraoral radiography preparation assessed as moderate to good level. Conclusions: The results showed that the main reason for the present problems is insufficient knowledge of the dentists in related subjects. Therefore, the solution, which is recommended among dentists, is to raise their awareness and to change their attitudes and culture in order to improve their performance.}, Keywords = {knowledge, attitude, education, infection control, intraoral radiography}, volume = {6}, Number = {4}, pages = {447-457}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-834-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-834-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Gholamzadeh, Saeeid and Akhghari, Maryam and Zarenezhad, Mohammad and Gharedaghi, Jaber and Montazeri, Mahmoud and Doroodchi, Alireza and Kazemi, Bahare}, title = {The Effect of Storage Longtime on Cyanide Production in Postmortem Stored Tissues}, abstract ={Background & Objective: Acute cyanide poisoning happens intentionally as suicide attempt or in accidental use. Interpretation of cyanide analysis results in postmortem samples is important in forensic medicine. Material & Methods: In this case-control study, the liver and the lung of 100 autopsy cases were collected during six months in Shiraz Forensic Medicine Department. Samples were divided into three parts and were examined as follow: one part of the liver and the lung was analyzed qualitatively to detect cyanide with Prussian blue test at first day of admission, the second and the third parts of the samples were stored at 0-4°C for one and two months, respectively. Then, liver and lung samples were analyzed to measure the amount of cyanide. Results: Cyanide was detected in only six cases in all three parts of the liver samples. Screening results for cyanide were negative for the remaining 94 liver samples. Conclusion: Endogenous cyanide production was not detected in liver and lung samples in this study. Therefore, cyanide detection was not affected by the time of the storage of the samples. The cyanide level changes in mentioned tissues were not significant. More importantly, our results would be more advantageous if we studied quantitatively on more samples to pave the way for future studies.}, Keywords = { Cyanide, Prussian Blue, Forensic Toxicology, Liver, Lung}, volume = {6}, Number = {4}, pages = {458-465}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-985-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-985-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Izadi, Homeira and Sohrabi, Nooshin and Tebianian, Majid and Mosavari, Nader and Mahdavi, Mehdi}, title = {Evaluation of Serum Specific Antibody against Recombinant ESAT-6 Antigen in Patients with Tuberculosis and Comparing to Normal Controls}, abstract ={Background & Objective: Tuberculosis (TB) is a zoonotic disease which is caused by Mycobacterium tuberculosis. Because of common structural and secretory antigens between pathogen and nonpathogenic mycobacterium, the specific diagnosis of TB is difficult. Therefore, it is very important to find a new method with high specificity and sensitivity for accurate and rapid diagnosis of tuberculosis. In this study, the serodiagnostic potential of Mycobacterium tuberculosis recombinant ESAT-6 in TB infected patients was evaluated by Enzyme Linked Immunosorbent Assay (ELISA). Materials & Methods: 55 TB patients with active disease and 28 healthy controls have been collected and evaluated in different dilutions in ELISA methods for the presence of specific anti-ESAT-6 antibody. The specificity and the sensitivity of this method was compared with the culture test. Results: TB patients have high levels of specific antibody against ESAT-6 antigens. The specificity and the sensitivity of this method was calculated as 80.90% and 85.45%, respectively. Conclusion: These findings provide useful information on the importance of ESAT-6 protein and suggested this serologic test as a good alternative method for rapid and prefect diagnosis of tuberculosis.}, Keywords = {Mycobacterium tuberculosis, ESAT-6, Antibody, ELISA}, volume = {6}, Number = {4}, pages = {476-480}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1147-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1147-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Ramouz, Ali and Hosseini, Mahdie and Ahmadiasrbadr, Yadollah}, title = {Comparison of Semen Analysis Parametes Changes after Priscription of Two Immunosuppresive Drugs, Tacrolimus and Cyclosporine in Men Undergoing Renal Transplantation}, abstract ={Background & Objective: The research objective was to investigate the prevalence of infertility in renal transplant patients receiving immunosuppressive agents and compare the impact of tacrolimus (target of Rapamycin inhibitors (TOR-I)) and Cyclosporine on spermogram parameters after the transplantation. Material & Methods: In this prospective study, from March 2013 to March 2014, all male patient who underwent renal transplantation and were treated with Tacrolimus or Cyclosporine, were included. Patients’ spermogram was analyzed in terms of sperm count and motility. Results: Average sperm count in patients receiving cyclosporine and tacrolimus before the transplantation was 1.73 × 106 and 2.08 × 106 and after the transplantation was 1.2 × 106 and 1.22 × 106, respectively (P=0.008)(P=0.002). Average percentage of the motile sperms of patients treated with Cyclosporine, before the transplantation and start of immunosuppressive course was 45.10%, which decreased to 40.50% at the end of the course. The analysis indicated a significant reduction in sperm motility (P=0.001). Average percentage of the motile sperms of patients receiving Tacrolimus, before the transplantation and start of treatment was 46% that decreased to 38% after the transplantation. This finding presents the significantly reduced sperm motility (P=0.002). Finally, the effective sperm motility in patients treated with Cyclosporine was significantly higher than patients treated with Tacrolimus. Conclusion: Tacrolimus reduces sperms’ motility in renal transplant patients significantly higher than Cyclosporine.}, Keywords = {Infertility, Cyclosporine, Spermogram, Tacrolimus}, volume = {6}, Number = {4}, pages = {481-487}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1019-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1019-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Hajighahramani, Shahi}, title = {A Comparison of Intramuscular Anesthetic Techniques in Chickens}, abstract ={Background & Objective: Administration of anesthetic substances to chickens requires careful consideration for the safe delivery of the agent to the bird. The research objective was to evaluate several drug combinations for intramuscular anesthesia in chickens for physiologic, nutritional, pharmacological and other investigations. Meterial & Methods: Sixty healthy chickens were randomly assigned in six treatment groups and received Ketamine in combination with Xylazine, Midazolam or Acepromazine. Heart and respiratory rate, induction time, duration of surgical anesthesia and light anesthesia were measured. Results: Induction of anesthesia was significantly longer following Acepromazine- Ketamine and Midazolam- Ketamine compared to other groups (P<0.05). Duration of surgical anesthesia was longest with Xylazine- Midazolam- Ketamine and shortest with Midazolam-Ketamine and Acepromazine- Ketamine (P<0.05). Conclusion: In conclusion, the most effective drug combinations resulting in longer duration of surgical anesthesia, were Xylazine- Acepromazine- Ketamine and Xylazine- Midazolam- Ketamine. Other combinations did not produce appropriate surgical anesthesia, but they make slight changes in physiological data.}, Keywords = { Anesthesia, Chicken, Ketamine, Acepromazine, Xylazine}, volume = {6}, Number = {4}, pages = {488-495}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1106-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1106-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Tavassoli, Zahra and RouhaniNejad, Hamideh and FallahMehrabadi, Jalil}, title = {Cloning and Expression of Nano Body Gene against Enterotoxin B of Staphylococcus Aureus}, abstract ={Background & Objectives: Staphylococcus aureus bacteria causes many different diseases by secretion of various enterotoxins. Therefore, it is necessary to develop ways that facilitate the detection of enterotoxins. Nowadays, immunochemical methods which are based on monoclonal antibody technology are used. The heavy chain antibodies that are called VHH or Nano body were found in blood serum of the Camelidae family. The unique properties of this antibody such as their binding to small molecules like toxins make them attractive candidates for the development of immunodiagnostic tests. The present study was done to achieve a VHH molecules against Staphylococcus enterotoxin B. Materials & Methods: Freighting phage library for isolate private Nano bodies against enterotoxin B was done in previous works. Next, pCANTAB 5E vector that consists VHH, extracted from E.coli bacteria strain xl1blue, and after doing PCR process with relative primers, sub cloning in pET21a(+) as an expression vector with cut sites NdeI and XhoI was done. Transformation in E.coli bacteria strain BL21(DE3) was done. Then, the cells effected with IPTG and producing time, and other terms were optimized. Finally, the expression of the protein with SDS-PAGE and western blot techniques was evaluated. Result: For proving cloning of nano body gene in pET21a (+) vector, nucleotide sequence of gene was analyzed, and transforming to E.coli bacteria strain BL21(DE3) was successful. After inspiration, active protein in cell was seen by SDS-PAGE technique and proved by western blot. Conclusion: cloning, sub cloning, and nonabody expression were surveyed in this research. Production of this protein can help to develop new therapeutic methods and produce vaccine against enterotoxin B of Staphylococcus aureus}, Keywords = {Nanobody, VHH, Entrotoxin B, Staphylococcus aureus}, volume = {6}, Number = {4}, pages = {496-503}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1004-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1004-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Esmaeili, Masomeh and Etemaddar, Narges and Gholampoor, Usef}, title = {The relationship between personality and coping style according to the mediating role of resilience in patients with}, abstract ={Background & Objective: This study tries to explain the role of mediator between personality characteristics and coping styles is resiliency. Material & Methods: The study population consisted of all patients is a member of the MS Society of Shiraz who were selected by convenience sampling. 3 tests were used to collect data: Connor-Davidson Resilience Scale, NEO Five-Factor personality inventory questionnaire Folkman and Lazarus coping strategies. Analysis of data obtained using SPSS (version 21) software and using Pearson correlation and regression analysis was performed. Results: High neuroticism in MS patients reduce resiliency (r=-0.279). Also people with MS deal with emotional problems just behave. Emotional coping style (p=0.023 & b=0.429) and the personality trait of neuroticism (p=0.046 & b=0.388) are able to predict Resiliency. Conclusion: Based on these findings, in addition to informing the people affected by psychological training, effective in reducing neuroticism, anxiety and consequently raise the level of resiliency.}, Keywords = {Personality characteristics, Coping style, Resilience, Multiple Sclerosis}, volume = {6}, Number = {4}, pages = {504-511}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-932-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-932-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Mohammadzadeh, Ali and KheiriHiagh, Ali and Vahedi, Maji}, title = {The Comparison of Perfectionism Components among Patients with Obsessive - Compulsive, Major Depression Disorders, and Normal People}, abstract ={Background & Objectives: Major depression and obsessive - compulsive disorders have some comorbidities. The objective of this study is to compare perfectionism and its components in patients with depression, obsessive - compulsive disorders and normal individuals. Materials & Methods: The current study was conducted in cross-sectional context.   The study population included all patients who referred to the clinics, psychiatric centers and counseling centers in the city of Tabriz. The sample was divided into three groups, one group of 30 with a diagnosis of obsessive - compulsive disorders, another group of 30 with a diagnosis of major depression disorder, and the other 30 were normal. Participants answered to Hill perfectionism questionnaire. To analyze the data, multivariate analysis of variance was used. Results: The results showed that the mean scores of perfectionism and its positive- negative dimensions were higher in patients with obsessive - compulsive and depression disorders, in comparison with normal individuals. Also no significant differences in perfectionism and its positive- negative dimensions scores was observed between people with these two mentioned disorders. Conclusion: The perfectionism components are common component in obsessive - compulsive and major depression disorders.}, Keywords = {perfectionism, obsessive - compulsive disorder, major depression disorder}, volume = {6}, Number = {4}, pages = {512-521}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-999-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-999-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {BerenjeianTabrizi, Hossein and Mirdar, Shadmehr and Moghanibashi, Mohhamad Mahdi and AnsariPirsaraei, Zarbakht}, title = {The Effect of High-Intensity Interval Training on Mitochondrial Biogenesis of Lung Tissue}, abstract ={Background & Objectives: It is well established that High-intensity Interval Training (HIT) may represent a time-efficient strategy to induce adaptations normally associated with endurance training. However, the effect of exercise on lung mitochondrial changes is not well understood.The purpose of the present study is to determine the effects of HIT on NRF-1 and PGC-1α genes at mRNA level in rat lung tissue. Materials & Methods: Twenty Wistar male rats (4 weeks old, 68±9 g weight) were randomly assigned to 6-week training, 9-week training, 6-week control and 9-week control groups. High-intensity interval training program was started with 25 m/min and gradually reached to 70 m/min at the end of the ninth week. Following tissue sampling, RNA extraction and cDNA synthesis, and the expressions of genes were determined by real time RT-PCR technique. Results: NRF-1 and PGC-1α genes expression were increased following interval training. The expression of NRF-1 and PGC-1α between 9-week training and 9-week control groups was significantly different (P≤0.05). Conclusion: According to the current study, it seems that intense interval training can cause changes in the mitochondrial content and the possibly of mitochondrial biogenesis in lung tissue.}, Keywords = { high-Intensity interval training, Mitochondrial biogenesis, Lung tissue}, volume = {6}, Number = {4}, pages = {522-529}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1211-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1211-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Tanideh, Nader and Nematollahi, Leila and Hosseini, Vahid and Hosseinzadeh, Masoud and Mehrabani, Davood and Safarpour, Alireza and Masoud, Sepehrimanesh and KoohiHosseinabadi, Omid and Najibi, Asm}, title = {The Healing Effect of Hydroalcoholic Extract of Hypericum Perforatum on Acetic Acid-Induced Ulcerative Colitis in Male Rats}, abstract ={Background & Objective: Anti-inflammatory effect of Hypericum have long been considered. Ulcerative Colitis (UC) is a form of Inflammatory Bowel Disease (IBD). In this study, the effects of Hypericum perforatum on histopathological changes and tissue malondialdehyde (MDA) level of colonic tissue in rats with induced UC were evaluated. Materials & Methods: 70 rats were divided into seven equal groups. Colitis was induced by acetic acid.. Groups I and II received 1 mL of 600 and 300 mg/kg H. perforatum extract orally per day respectively; groups III and IV received 1 mL of 20% and 10% intra-colonic gel form of H. perforatum extract daily respectively; group V, as positive control, received 1 mL of intra-colonic Asacol; group VI received 1 mL of normal saline as negative control; group VII received just intra-colonic gel base. All the animals were evaluated for histological changes and tissue MDA level of colon seven days after the treatment. Results: H. perforatum extract in the two forms of trans-rectal and oral administration could result in a more healing effect on acetic acid-induced damaged colonic tissue with a reduction in the MDA activity. In trans-rectal administration, the 20% gel had a better healing response than the 10% gel. In oral administration, the 600 mg/kg dosage had a better healing response than the 300 mg/kg. Conclusions: Therefor, H. perforatum can be considered as a treatment of choice for UC especially in trans-rectal gel form.}, Keywords = {hypericum perforatum, inflammatory bowel disease, ulcerative colitis, Malondialdehyde}, volume = {6}, Number = {4}, pages = {530-537}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-978-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-978-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Babazadeh, Towhid and Kiani, Amin and Moradi, Fatemeh and Shariat, Fariba and BanayeJeddi, Mortez}, title = {The effect of self-care behaviors education on quality of life in patients with type 2 diabetes: a randomized clinical trial}, abstract ={Background & Objectives: Diabetes is the most common metabolic disorder which if not controlled; it can cause a decrease in patient’s quality of life. Therefore, this study was carried out to evaluate the effect of educational intervention of self-care behaviors on quality of life in patients with type 2 diabetes. Materials and methods: This clinical trial study was conducted in 2015 on 90 patients with Type II diabetes in the city Chalderan. Patients were stratified randomization to intervention and control groups. Data was collected using standardized questionnaires in two phases before and 3 month after the educational. To analyze the data was used descriptive statistics and paired t and independent t tests in level of p<0.05. Results: Mean and standard deviation age of the patients was 46.3 ± 9.5. The results showed that the mean (SD) score of knowledge, attitudes and self-care behaviors significantly has increased in intervention group, but changes was not significant in the control group. Also, the educational intervention had a significant effect in improving the quality of life in all its dimensions compared to the control group had (p<0.05). Conclusion: The findings of current study showed that educational intervention has been improved self-care behaviors in patients with type II diabetes. Also the results showed that by training self-care behaviors can be improved quality of life.}, Keywords = {Educational intervention, Self-care, Type II diabetes, Quality of life}, volume = {6}, Number = {4}, pages = {538-547}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1111-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1111-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} } @article{ author = {Sholehvar, Fatemeh and Asadzadeh, Azizeh and Seyedhosseini, Hoori}, title = {Molecular Docking Studies of Some Hydroxy Nitrodiphenyl Ether Analogues as Tyrosinase Inhibitors}, abstract ={Background & Objective: Tyrosinase is a key enzyme in pigment synthesis.Overproduction of melanin in parts of the skin results in hyperpigmentation diseases. Thus, its inhibitors are highly important in the medical, cosmetic and agricultural fields. The aim of this research is the bioinformatical study of tyrosinase inhibition by a number of hydroxy nitrodiphenyl ether derivatives. Material & Methods: This is a descriptive-analytic study. In order to investigate the mode of interaction of the compounds with tyrosinase active site, the chemical structures of all compounds were designed using ChemDraw program, then transferred into Hyperchem software for energy minimization. Docking study was performed by AutoDock 4.2 program and the resulting docking poses were analyzed in AutoDockTools, DS Visualizer 3.5 and Ligplot software. Results: Among the all studied compounds, the best docking results were related to 4-Hydroxy- 2'-nitrodiphenyl etherdisplayed. In fact, this compound had the most negative ΔGbind (-12.79 Kcal/mol) that indicated favorable interactions with the key amino acid residues at active site of tyrosinase. Docking results for this compound are in accordance with those of co-crystallized ligand (tropolone). In this compound, the oxygen of nitro group has an efficient metal-ligand interaction with the Cu2+ ions in the active site. Conclusion: Finally, in respect to  high effectiveness and docking results, we can conclude that the compound 4-Hydroxy- 2'-nitrodiphenyl ether may be regarded as an effective anti-tyrosinase inhibitor.}, Keywords = {Bioinformatic, Docking, Hydroxy Nitrodiphenyl Ether, Tyrosinase}, volume = {6}, Number = {4}, pages = {548-555}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-1097-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-1097-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2016} }