@article{ 
author = {Ranjbar, Mohammad Mehdi and Yousefi, Ali Reza and Motedayen, Mohammad Hasan and Molazadeh, Shima and Karimi, Gholamrez},  
title = {Disease Prevention, Genetic Selection, and Vaccination Based on BoLA-DRB3.2 Polymorphism: A Model for Immunogenetic Studies}, 
abstract ={ Unearthing the immune defects associated genes and genetic variations may lead to locating novel targetable elements and introducing the underlying mechanisms and pathways of the desired condition. The major histocompatibility complex (MHC) genes are essential for protein antigens presentation and inducing immune response as well as are associated with production and phenotypic traits. The MHC class II genes of cattle and buffaloes, Bovine Leukocyte Antigen (BoLA) or Buffalo Leukocyte Antigen (BuLA), are located on the short arm of chromosome 23. It has been demonstrated that the second exon of BoLA-DRB3 (BoLA-DRB3.2) is highly polymorphic, having more than one hundred identified alleles, that each of them can form special binding pockets for corresponding antigenic peptides. Concerning the populations of cows, unique native and hybrid, and buffaloes are distinctly divided into different regions of Iran, analysis and interpretation of the polymorphisms’ expression status of this locus can be implemented to find better breeding strategies like selecting highly resistant animals to infection diseases, in herds. It also can help to develop effective and novel vaccines regarding allele frequencies in populations; different allelic variants of MHC class II binding to different peptides. Immunogenic evaluation of animals’ genome/genes characteristics has always been used as a model for the study of similar genes in humans.},  
Keywords = {Polymorphism, BoLA-DRB3, MHC class II, Immune response, Iran},
volume = {12},
Number = {1}, 
pages = {1-11}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i1.8867},
url = {http://jabs.fums.ac.ir/article-1-2702-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2702-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Owlia, Fatemeh and Hekmatimoghaddam, Seyed hossein and Kheirollahi, Faraz},  
title = {Could Frequency of Candida spp. Be Different on Various Parts of Tongue in Denture and Non-denture Wearers?}, 
abstract ={Background & Objectives: Oral Candidiasis is the most common opportunistic fungal infection that could affect the oral mucosa. Studies to date did not compare the colony count of candida of the anterior and posterior surfaces of the tongue. This study makes an attempt to compare the Candida spp. on the anterior and posterior surfaces of tongue among healthy denture and non-denture wearers. Materials & Methods: Participants of the current cross-sectional study were 26 healthy denture wearers (DW) and 10 healthy non-denture wearers (NDW).  Oral specimens were collected from anterior and posterior tongue dorsa by swabbing for mycological examination. After 48 h incubation on Sabouraud dextrose agar (S) and chloramphenicol (SC) and chloramphenicol and Cycloheximide (SCC) ™ medium, C. Albicans and non-Albicans were identified by Germ Tube test. Isolated colonies were evaluated. Obtained data were analyzed by SPSS software version 17. Mann-Whitney and Wilcoxon nonparametric tests were used for statistical comparison of data due to non-compliance with normal distribution. Results: The higher rate of isolated colonies was seen in DW group compared to NDW group (P-Value = 0.03). There was a significant difference between candida colonization of anterior and posterior surface of tongue (P-Value = 0.006). C. Albicans was the most common isolated candida species. Conclusions: Mycological findings of this study revealed that the presence of denture can increase colonization of candida on the posterior surface of the tongue. It could be an important role in choosing the best form for medical management of oral candidiasis},  
Keywords = {Candida Albicans, colonization, Age, denture},
volume = {12},
Number = {1}, 
pages = {12-20}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i1.8868},
url = {http://jabs.fums.ac.ir/article-1-2692-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2692-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Soltani, Negar and Pishkar, Leila and Chaleshi, Vahi},  
title = {Evaluation of NKILA lncRNA and NFκB Genes Expression in an Iranian Gastric Cancer Patient Compared with Healthy Tissue and the Relationship between Clinicopathological Features}, 
abstract ={Background & Objective: Long non-coding RNAs play critical roles in the initiation and development of gastric cancer (GC). The aim of this study was to assess the expression of NKILA and NFKB genes and the relationship between their expressions with clinical characteristics in gastric cancer. Also, relative expression of lncRNAsNKILA, compared NFKB in GC tissues was evaluated. Materials & Methods: This cross-sectional study was performed on 50 gastric formalin-fixed paraffin-embedded tumor tissue samples and 50 normal tissues. The RNA level of lncRNAsNKILA and NFKB genes was assessed using the quantitative real-time polymerase chain reaction. B2M was used as an internal control. The 2 -ΔΔCq method was used to determine the expression fold changes. Results: A significant association was observed between the levels of NKILA, in gastric tumor tissues compared with normal tissues (Mean = 2.087, p= 0.0484). The relative expression of the NFKB gene revealed no statistically significant difference between the gastric tumor tissues compared with normal tissues (P=0.3728). In addition, clinicopathologic data compared with NKILA and NFKBmRNA expression levels in gastric cancer tissues showed no significant association. Here, we found no significant association between the level of NKILA expression compared with NFKB mRNA level in gastric cancer tissues (R=0.03; P=0.2355). Conclusion: Our results showed that NKILA had a significant association with GC. Our findings also revealed that NKILA expression was not correlated with NFKB mRNA level in GC tissues.  },  
Keywords = {Gastric Cancer, NKILA, NFKB, Clinicopathological Feature, LncRNA},
volume = {12},
Number = {1}, 
pages = {21-29}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i1.8869},
url = {http://jabs.fums.ac.ir/article-1-2683-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2683-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Teimouri, Maryam and pooladi, Mehdi},  
title = {Toxicity effects of lipid nanoparticles containing Artemisia absinthium L, essential oil on BRC-03 breast cancer cell line}, 
abstract ={Abstract Background & Objective: According to research, breast cancer is the second most common cancer among women. Nanomaterials of various materials significantly increase the solubility, stability and effective drug delivery, and in recent years research on the effectiveness of essential oils and plant extracts on inhibiting the growth of cancer cells is expanding. Artemisia belongs to the Asteraceae family. In many studies, the effectiveness of the essential oil and extract of this plant on inhibiting the growth of cancer cells has been reported. Materials & Methods: In this experiment, nanoparticles containing Artemisia absinthium L essential oil (thistle) were first synthesized by homogenizer & sonication method and then their physicochemical properties were determined, such as particle size, particle size distribution, zeta potential, percentage of loading efficiency. Essential oils, particle shape. Then, the anti-apoptotic effect of lipid nanoparticles containing Artemisia essential oil in breast cancer cells (BRC-03) was evaluated. Results: The results of cellular effect of lipid nanoparticles containing essential oil of Artemisia absinthium L (thistle) on breast cancer cells showed that increasing the concentration of lipid nanoparticles containing essential oil of this plant reduced the survival rate of cancer cells. Breast (BRC-03) was purified compared to essential oil (p <0.05). Conclusion:  Nano-essential oil of this plant is effective in reducing the IC50 of the drug and increasing the cytotoxicity of the essential oil of the plant. Lipid nanoparticles containing essential oil of Artemisia absinthium L (thistle) activated the apoptotic pathway in breast cancer cells.},  
Keywords = {Breast cancer, Artemisia absinthium L, Anti-apoptotic, nanoparticles},
volume = {12},
Number = {1}, 
pages = {30-40}, 
publisher = {Fasa University of Medical Sciences},
url = {http://jabs.fums.ac.ir/article-1-2713-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2713-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {MehdizadehTazangi, Roya and Bizhani, Mostafa and Karimi, Shanaz and Naghizadeh, Mohammad Mehdi and KhaniJeihooni, Ali and Rahimzahedi, Mohammad Hossei},  
title = {The Effectiveness of Peer Group-Based Training in Adherence to Treatment of Adults with Diabetic Foot Ulcer}, 
abstract ={Background & Objective: Adherence to treatment of patients with diabetic foot ulcer leads to successful treatment and a reduction in the severity of complications. The present study was conducted to investigate the effect of peer group-based training on adherence to treatment of adults with diabetic foot ulcers in Shiraz in 2020. Materials & Methods: This quasi-experimental study was conducted on 70 patients (35 in the intervention group and 35 in the control group). The content of the education program was performed by the peer for the intervention group in 5 sessions of 45 minutes, and the routine education of the diabetes clinic was provided to the control group. Data collection tools included demographic characteristics questionnaire, Morisky Medication Adherence Scale (MMAS). Data were analyzed by the SPSS software version 22 using statistical tests (t-test, ANOVA and Chi-square) and the significance level was considered p<0.05. Results: The mean score of adherence to treatment was significant immediately after the intervention, but there was no significant difference after one and three months. Conclusion: Peer training of patients with diabetic foot ulcers could improve adherence to treatment. Therefore, health system policymakers may use this approach as an effective method in the care program of patients with diabetic foot ulcers and other diseases.  },  
Keywords = {Therapeutic Adherence and Compliance Peer group, Diabetic foot.},
volume = {12},
Number = {1}, 
pages = {41-50}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i1.8871},
url = {http://jabs.fums.ac.ir/article-1-2705-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2705-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Keysan, Parya and Ghorbian, Saei},  
title = {Evaluation of the Long noncoding RNA NONHSAT037832 Gene Expression Changes in Patients with Thyroid Carcinoma}, 
abstract ={Background & Objective: Thyroid cancer is the most common endocrine disorder in the world. One of the main causes of this cancer is genetic changes, in particular mutations in the tumor and oncogene inhibition genes. In addition to mutations, some non-encoding RNAs called lncRNAs play an important role in the progression of tumors. NONHSAT is a lancer and has recently been referred to the role of this gene in various types of cancer. This study aimed to evaluate the lncRNA-NONHSAT037832 gene expression changes in patients with thyroid carcinoma. Materials & Methods: A cross-sectional study was conducted on 140 homogeneous samples (70 cancer tissue homogeneous samples and 70 homogeneous healthy tissue samples) from patients with thyroid cancer referred to Alzahra Hospital in Tabriz. Using the Real-Time PCR, changes in the expression of the lncRNA-NONHSAT037832 gene were evaluated. In addition, the relationship between lncRNA-NONHSAT037832 gene expression changes and clinical parameters was investigated. Results: The results showed that the expression of lncRNA-NONHSAT037832 gene expression in the tissues of cancer was significantly higher than healthy tissues (P <0.001) and a significant relationship with MTN (P = 0.001) and node metastasis Lymphoma was shown (P = 0.30). Conclusion: According to the results, the lncRNA-NONHSAT037832 gene may be used as a prognostic biomarker for thyroid cancer.  },  
Keywords = {LncRNA, lncRNA-NONHSAT037832, Thyroid carcinom},
volume = {12},
Number = {1}, 
pages = {51-59}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i1.8872},
url = {http://jabs.fums.ac.ir/article-1-2531-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2531-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Ranaiy, Mohammad Saleh and Farokhi, Farah and BabaeiBalderlou, Farri},  
title = {The Effect of Simvastatin and Vitamin D Co-Administration on Rats Brain Function and Behavior: A Behavioral and Biochemical Study}, 
abstract ={Background & Objective: The simvastatin (Sim) is a lipophilic statin and can cross the blood-brain barrier. The role of vitamin D (Vit D) in brain development and function has been supported over the past decade. This study aimed to evaluate the effect of simvastatin on memory and anxiety levels in healthy male rats. Materials & Methods: In this experimental study, 36 male Wistar rats weighing 250-300 g were randomly divided into six groups (n=6) including control, Vit D (5 µg/kg/day; IP), Simvastatin (1 mg/kg/day; orally) (SimL), Simvastatin (10 mg/kg/day; orally) (SimH), SimL + Vit D and SimH + Vit D. After 28 days, at the end of the treatment, the behavioral anxiety test and memory behavioral test were performed. Then, the rats were euthanized, and oxidative stress markers of the brain, serum low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and cholesterol levels were investigated. Data were analyzed by SPSS 24 software and Tukey’s test. Results: Co-administration of simvastatin and vitamin D significantly increased working memory, catalase activity, total antioxidant capacity, HDL-C, and decreased anxiety levels, malondialdehyde (MDA) concentration, cholesterol, and LDL-C compared to the control group (p<0.05). However, the administration of simvastatin and vitamin D alone did not significantly change the mentioned parameters compared to the control group (p>0.05). Conclusion: Co-administration of simvastatin and vitamin D can improve brain function by reducing oxidative stress and cholesterol levels.  },  
Keywords = {Anxiety, Simvastatin, Vitamin D, Working memory, Rat},
volume = {12},
Number = {1}, 
pages = {60-68}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i1.8873},
url = {http://jabs.fums.ac.ir/article-1-2718-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2718-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {RazaviEbrahimi, Parham and TabatabaeiNaeini, Aboutorab and RaayatJahromi, Alireza and Amini, Masood and Zare, Afshi},  
title = {The Effect of Vertical Sleeve Gastrectomy on Weight Loss, Insulin and Glucose Levels in Dogs}, 
abstract ={Background & Objective: This study was designed to evaluate the effect of sleeve-gastrectomy procedure on weight loss, glucose and insulin blood levels in overweight dogs. Materials & Methods: In the current study, 5 overweight dogs (31.6±3.03kg) were selected for sleeve-gastrectomy procedure. Insulin and glucose levels were measured from the blood samples that were taken before the surgery and weekly 1.5 month post-operatively. The weight of the dogs was also measured at the same time. The surgery was performed according to the technique described by Gagner 2009, and the dogs received special diet during the recovery period to end of the study. Results: One dog died due to dehiscence and leakage 48 hours post-operatively. Decrease in mean weight was 6.25±0.44 in the first week after surgery and the weight loss process continued to end of the study. Changes in insulin and glucose blood levels demonstrated to have pulsatile patterns. Insulin levels decreased for the first week, but its level increased in the second week period. Again, insulin levels demonstrated to have decreasing pattern in the following 4 weeks. On the other hand, the glucose levels changed each week and did not show any certain pattern. Conclusion: Post-operative weight loss was observed in all cases but it was not statistically significant. It could be due to limited number of the samples. The glucose and insulin changes were attributed to hormonal changes particularly gut hormones that were affected by sleeve-gastrectomy procedure but further detailed studies on hormonal effect are required.  },  
Keywords = {obesity, Sleeve Gastrectomy, insulin, blood glucose, dogs},
volume = {12},
Number = {1}, 
pages = {69-78}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i1.8874},
url = {http://jabs.fums.ac.ir/article-1-2688-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2688-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Molazadeh, Shima and Keivanfar, Hadi and Bakhshesh, Mehran and NikbakhtBrujeni, Gholamrez},  
title = {Epitope Identification in BEFV Gene for Detecting Effective Points}, 
abstract ={Background & Objective: The most common cause of milk production loss in cattle and water buffalo is bovine ephemeral fever (BEF). Previous cases have been reported in Iran's south regions, with a low mortality rate. As a result, studying BEFV and identifying ideal epitopes for further developing diagnostic paths is important. Materials & Methods: To investigate BEFV N protein epitopes, we collected samples, extracted and sequenced DNA, and then used the ExPaSy translate method to deduce the amino acid sequence. Various immunoinformatics techniques were used to analyze physical/chemical properties, secondary structure of protein sequences, membrane topology, antigenic property, and 3D structure. BCPRED and the DiscoTope server, respectively, predicted linear and discontinuous epitopes of BEFV N protein. Finally, the PatchDock server was used to dock peptides and antibodies. Result: Three linear epitopes and sixteen discontinuous epitopic positions were discovered. Furthermore, molecular docking between epitopes and low-binding-energy antibodies revealed that they have easy access to the immune system. Conclusion: In this study, bioinformatics techniques were used to predict epitopes of the BEFV N protein for further developing BEFV diagnostic paths. Furthermore, experimental validation is needed for these epitopes.  },  
Keywords = {Epitopes, Immunoinformatics, Docking},
volume = {12},
Number = {1}, 
pages = {79-87}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i1.8875},
url = {http://jabs.fums.ac.ir/article-1-2681-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2681-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Safarzadeh, Hamed and Zargari, Felor},  
title = {Effects of Melatonin on Hepatic and Renal Complication of Diabetes}, 
abstract ={Background & Objective: Diabetes is associated with biochemical and pathological changes in liver and kidney tissue. One of the most important mechanisms in causing   complications of diabetes is increasing the production of free radicals due to impaired glucose metabolism. Some studies have shown that melatonin is effective in protecting tissues due to its antioxidant role. The aim of this study was to evaluate the effect of melatonin on biochemical factors, histopathological changes of liver and kidney in streptozotocin-induced (STZ) diabetic rats. Materials & Methods: In this experimental study, 40 adult male rats were randomly divided into 4 groups (n=10): control group, diabetic group, melatonin group (20mg/kg BW) and melatonin + diabetic group.  At the end of the 6th week, blood samples were collected. Biomarkers of liver function (alkaline phosphatase: ALP, aspartate aminotransferase: AST, alanine aminoteransferase: ALT) and kidney biomarkers (urea, uric acid, BUN, creatinine), serum glucose and histopathological changes of these tissues were evaluated.  Data analysis was performed using SPSS and the significance level was P<0.05. Results: Melatonin treatment significantly (P<0.05) reduced hyperglycemia, hepatic enzymes and renal biochemical factors due to diabetes and improved histopathological changes of liver and kidney tissue. Conclusions: Melatonin consumption can be effective in improving the renal and kidney complications of diabetes.    },  
Keywords = {melatonin, liver, kidney, diabetes},
volume = {12},
Number = {1}, 
pages = {88-93}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i1.8876},
url = {http://jabs.fums.ac.ir/article-1-2721-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2721-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Dabirinejhad, Hakime and Dayer, Mohammad Reza and Mohammadi, Tayebeh},  
title = {Effect of Zinc Supplementation on some Biochemical and Hematological Parameters in Alloxan Induced Diabetic Rats}, 
abstract ={Background & Objective: This study aims to evaluate the effects of zinc sulfate on blood biochemical and hematological parameters in healthy control and alloxan-induced diabetic rats.  Materials & Methods: Experimentally, 40 rats were used in four equal groups, including healthy control, healthy feed with zinc sulfate, diabetic control, and zinc sulfate. The alloxan 120 mg/kg with blood sugar over 140-250 mg / dL was intraperitoneally injected to induce diabetes mellitus. In addition, the value of 0.6 g / L zinc sulfate dissolved in tap water was used for three months to be drunk by the treated group. All animals were killed, and blood and pancreas samples were collected for necessary tests. Serum levels of alpha-amylase, total cholesterol, triglycerides, LDL, HDL, hemoglobin, and hematocrit were measured. Statistical data were analyzed based on the analysis of variance using SPSS software. Results: Our data showed that in the diabetic control group, serum levels of total cholesterol, triglyceride, LDL increased significantly and hemoglobin, hematocrit, and HDL decreased significantly compared to the healthy control (P< 0.05). Zinc sulfate consumption in these groups reduces total cholesterol, triglyceride, LDL and increases hemoglobin and hematocrit compared to the control groups. Microscopic examination of the pancreas showed that in the diabetic control group, the percentage of Beta cells was reduced compared to the other groups. Conclusion: Our findings reveal that dietary zinc sulfate not only exerts no damage to bodies but also zinc sulfate as food supplement seems to have beneficial effects on biochemical and hematological parameters in diabetic and healthy animals.   Keywords: Alloxan, Zinc Supplementation, Biochemical parameters, Diabetic mellitus},  
Keywords = {Alloxan,  Zinc Supplementation, Biochemical parameters, Diabetic mellitus},
volume = {12},
Number = {1}, 
pages = {94-105}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i1.8877},
url = {http://jabs.fums.ac.ir/article-1-2722-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2722-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Sajadi, Seyyed Mehdi and Katiraee, Farzad and Shahbazfar, Amir Ali and TayefiNasrabadi, Hossein and FakhriDemeshghieh, Aliasghar},  
title = {Cytotoxicity of Trichoderma Reesei Extract in Hepa1-6 Cancer Cells}, 
abstract ={Background & Objective: In the last decades, many successful anticancer fungal metabolites have been obtained, and fungi have shown great potential to produce beneficial anticancer drug compounds. In this article, the effects of the reference strain of Trichoderma reesei ethanolic extract on the Hepa1-6 cell line were studied. Materials & Methods: The fungus was initially cultured in the potato dextrose agar media, and then, it was transferred to yeast peptone dextrose broth. Afterward, the ethanolic and aquatic extracts were prepared, and were analyzed by chromatography and mass spectrometry (GC-MS). Then, the cell lines were treated with different doses of the extracts. The cytotoxic activity of fungal extracts were evaluated by MTT assay, Ferric reducing ability of plasma (FRAP) assay and Ferric Reducing Antioxidant Power (FRAP) Assay. Alkaline phosphatase (ALP) and Lactate dehydrogenase (LDH) measurement tests were done on the culture and cell fractions. Hematoxylin & Eosin staining was performed for microscopic studies, and an oxidant attack-antioxidant defense test was performed on the cell fraction. Results: GC-MS determined compounds such as cineal, cyclohexene, hydroxyethyl benzaldehyde, terpinenyl acetate, dihydro esthophyllene, propionic acid, hexadecanoic acid, and octadecanoic acid were extracted from the fungal extract. The half maximal inhibitory concentration (IC50) was between 7-8%, and the highest cytotoxic effectiveness was 10-15%. An increase in the levels of ALP, LDH enzymes, and total protein was observed. Conclusion: Therefore, the findings suggest that the extract of T. reesei has inhibitory effects on Hepa1-6 cancer cell lines.},  
Keywords = {Cytotoxicity, Hepa1-6 cell line, Trichoderma reesei, ethanolic extract},
volume = {12},
Number = {1}, 
pages = {106-118}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i1.8878},
url = {http://jabs.fums.ac.ir/article-1-2591-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2591-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Shajareh, Mahsa Al-Sadat and Dastpak, Arezoo and Emtyazjoo, Mozhgan and Mirzaie, Amir},  
title = {Green Synthesis of Silver Nanoparticles Using Nasturtium officinale L. Extract And Analysis of Their Antibacterial Activity Against Nosocomial Pathogens}, 
abstract ={Background & Objective:  The green synthesis of the silver nanoparticles (AgNPs) is an eco-friendly and straightforward synthesis method. This study aimed to investigate the green synthesis of AgNPs using Nasturtium officinale leaf extract and analysis of their antibacterial activity against some nosocomial pathogens. Materials & Methods: The obtained AgNPs were characterized using UV-visible spectroscopy, X-ray diffraction (XRD), and scanning electron microscope (SEM). In addition, the antibacterial activity of synthesized AgNPs and N. officinale leaf extract were performed against five bacterial strains (Staphylococcus  epidermidis, Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa) by agar disk diffusion assay and minimum inhibitory concentration test. Results: The green synthesized AgNPs had significant antimicrobial activity against all studied bacteria. In addition, greater inhibition activity was observed against E. coli and P. aeruginosa rather than other bacteria. Conclusion: The synthesized AgNPs revealed a special antibacterial effect against selected bacteria, compared to leaf extract of N. officinale. Identifying new antibacterial agents with profound efficacy against antibiotic-resistant opportunistic pathogens is essential. These data provide an eco-friendly and rapid green approach for AgNPs synthesis and the potential of AgNPs for use in drug development against nosocomial infections.  },  
Keywords = {Nosocomial pathogens, Silver nanoparticles, Nasturtium officinale},
volume = {12},
Number = {2}, 
pages = {119-131}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i2.9875},
url = {http://jabs.fums.ac.ir/article-1-2736-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2736-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Hosseinzadeh, Masoumeh and Janbozorgi, Maryam},  
title = {Relationship between Physical Activity and Coping Styles in the Face of Covid-19 Epidemic among Women in Ahvaz}, 
abstract ={Background & Objective: Decreased levels of physical activity during the COVID-19 epidemic can cause physical and psychological problems for the individual. Therefore, the aim of this study was to evaluate the relationship between physical activity and control coping styles in the face of the Covid-19 epidemic in Ahvaz, Iran. Materials & Methods: This study is a descriptive-analytical cross-sectional study and the research population includes females in Hamidiyeh Ahvaz, Iran, who were studying in 2019-2020. In this regard, 208 females were selected based on Cochran's formula and random sampling for participation in the study. Beck's physical activity questionnaires and Coping Styles Questionnaire in the face of the COVID-19 epidemic were used to collect data. Pearson correlation coefficient was used to establish statistical relationships. Results: The results only showed a significant relationship between social support coping style and physical activity (physical activity related to leisure time) (r= 0.142, p= 0.044). There was no significant relationship between physical activity (related to work, sport, leisure time and total) and any of the other coping styles (Confrontation, Avoidance, Self-control, Responsibility, Escape and avoid, Problem solving, Positive estimation) (p≥0.05). Conclusion: During the COVID-19 epidemic, physical activity related to leisure time was positively associated with social support coping style but all other cases we did not observe a significant relationship between physical activity and coping styles with Covid-19.  },  
Keywords = {COVID-19, women, coping styles, Physical Activity},
volume = {12},
Number = {2}, 
pages = {132-139}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i2.9876},
url = {http://jabs.fums.ac.ir/article-1-2756-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2756-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Shahidi, Samanehsadat and TahmasebiFard, Zahr},  
title = {Impacts of BMI & IL-4 Genetic Polymorphisms (rs2243250 C/T & rs2227284 A/C) on Iranian Breast Cancer Patients: a Pilot Study}, 
abstract ={Background & Objective: Breast cancer is a phenotypically complex and diverse genetic disease caused by changes in the structure and expression of specific genes. Immune system factors are also involved in the etiology of this neoplasm. This study aimed to evaluate the effect of genetic changes (rs2243250 & rs2227284) on the interleukin 4 gene and body mass index on breast cancer risk in Iranian women. Material & Methods: From women referring to Shohada-Tajrish Hospital, 100 women with breast cancer and 100 healthy women were selected. After blood sampling and DNA extraction, the women’s genotypes were determined using the RFLP-PCR technique. The results were evaluated by SPSS software version 21 and chi-square and logistic regression tests. Results: Analysis of the results with different genetic models showed the effect of rs2243250 on breast cancer (p<0.05), but rs2227284 was not associated with breast cancer (p>0.05). People with the CC / TT genotype (polymorphism) were more likely to get breast cancer. Also, the increase in body mass index was significantly associated with both polymorphisms studied. Also, carriers of the TT genotype of rs2243250 polymorphism were more likely to develop breast cancer with aging. Conclusion: Genetic alterations in the IL-4 gene and obesity probably contribute to breast cancer, and carriers of both genetic modifications (CC / TT) are more likely to develop breast cancer.  },  
Keywords = {Interleukin 4, rs2243250, rs2227284, Breast Cancer, Polymorphisms},
volume = {12},
Number = {2}, 
pages = {140-151}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i2.9877},
url = {http://jabs.fums.ac.ir/article-1-2767-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2767-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Karimi, Merat and Sadeghi, Ehsan and Zahedifar, Mostaf},  
title = {Carbon Quantum Dots Extracted from Natural Lemon Juice: Efficient Material for Fluorescence and Antibacterial Applications}, 
abstract ={Background & Objective: In recent years, the proliferation of microbial organisms has increased alarmingly, and the overuse of various antibiotics against microorganisms has increased drug resistance. On the other hand, the need to reduce health costs, the production of antimicrobials with low costs, and the basic needs of today's human society have become. This led to a large-scale study of new drugs against microorganisms and the use of nanoparticles as antibacterial agents were considered. This study aimed to use biocompatible carbon quantum dots(CQDs) nanoparticles instead of antibiotics resistant to gram-positive and gram-negative microorganisms. Materials & Methods: Fluorescent carbon quantum dots were extracted from natural lemon juice using the hydrothermal approach. Analyzes of X-ray diffraction (XRD), Fourier transform infrared(FT-IR), ultraviolet-visible (UV-Vis), photoluminescence(PL), transmission electron microscope(TEM), and energy-dispersive spectrometer(EDS). CQDs were investigated on ten types of microorganisms by the microwell dilution method. In this study, the minimal inhibition concentrations) MIC) and the minimum bactericidal concentration(MBC) was determined. Results: Fluorescent CQDs less than 5 nm in size were fabricated and confirmed by structural and microscopic analysis. This test showed that four microorganisms B. subtilis, E. coli, P. aeruginosa, S. pyogenes and C. albicans were resistant to the antibiotic nystatin and showed the highest sensitivity to CQDs, the lowest MIC and MBC for CQDs are 250 µg/ml and 1000 µg/ml. Conclusions: In general, the results obtained from this study can claim that CQDs have antibacterial properties and can be introduced after further studies as candidates are used to treat or prevent a variety of infections caused by microorganisms.},  
Keywords = {Antibacterial activity, hydrothermal, carbon quantum dots, lemon juice},
volume = {12},
Number = {2}, 
pages = {152-161}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i2.9878},
url = {http://jabs.fums.ac.ir/article-1-2766-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2766-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Delazar, Zeinab and AshrafiRahaghi, Mahrouz and SadeghiDinani, Masou},  
title = {Antileishmanial Activity of  New Steroidal Saponin Isolated from the Flowers of Allium Austroiranicum}, 
abstract ={Background & Objective: Plants are reservoirs of bioactive compounds, which are known to be chemically balanced, effective and least injurious as compared to synthetic medicines. The current resistance and the toxic effects of the available drugs have brought the trend to assess the antileishmanial effect of various plant extracts and their purified compound/s. Alliums are rich sources of steroidal saponins, flavonoids, and sulphuric compounds of which steroidal saponins have recently received more attention due to their important pharmacological activities. Allium austroiranicum is a common edible vegetable in western regions of Iran, especially in "Chaharmahal and Bakhtiari" province, where it is named “Lopo” and is considerably used as a raw vegetable, flavoring agent, and as a medicinal plant. Materials & Methods: The chloroform-methanolic extract was fractionated using MPLC, and the appropriate fractions were then subjected to isolation and purification of the constituents by HPLC. Structure elucidation was done using comprehensive spectroscopic methods including 1D and 2D NMR. Antileishmanial effects of the isolated compound against the promastigotes of Leishmania major were evaluated using MTT method. Results: Phytochemical investigation of chloroform-methanol extract of the plant resulted in the isolation and identification of a Nicotianoside C related steroidal saponin and its chemical structure was determined as (25S)-5α-Spirostan-1β,3β-diol-3-O-{α-L-rhamnopyranosyl-(1→2)-{α-L-rhamnopyranosyl-(1→4)}-[β-D-glucopyranoside}. Investigation of in vitro antileishmanial activity of the isolated compound, in 10 and 50 and 100 μg/mL concentrations, exhibited significant leishmanicidal effects against the promastigotes of Leishmania major. Conclusion: The results established a valuable basis for further studies about A. austroiranicum and anti-parasitic activity of steroidal saponins.  },  
Keywords = {Allium austroiranicum, Antileishmania, Saponin, Structure elucidation},
volume = {12},
Number = {2}, 
pages = {162-170}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i2.9879},
url = {http://jabs.fums.ac.ir/article-1-2727-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2727-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Ebadifar, Amir Mohamad and Jaydari, Amin and Shams, Nemat and Rahimi, Heidar},  
title = {Prevalence of Listeria Monocytogenes in Raw Milk of the Healthy Cattle in Lorestan Province (Iran) by PCR}, 
abstract ={Background & Objective: The prevalence of Listeria monocytogenes (L. monocytogene) in milk and dairy products, the high complications and damage associated with zoonotic Listeriosis have led to this bacterium being considered as a risky infection for public health. Materials & Methods: In the present descriptive and cross-sectional study, to investigate the prevalence of L. monocytogene, 100 samples of raw milk obtained from industrial farms in Lorestan province in winter and spring of 2019-2020 were collected and 16SrRNA and hlyA genes were detected using PCR reaction to identify Listeria genus and L. monocytogene species, respectively. Results: Based on the observed results, three samples (3%) were positive for Listeria genus, while no report of L. monocytogens species was observed. Conclusion: The results of this study did not show evidence of the presence of L. monocytogenes in raw cow milk, which indicates the sanitation and desirable nutritional conditions in industrial farms. Therefore, due to the presence of Listeria (other species of Listeria) in raw milk, if you consume raw and unpasteurized milk, the risk of human infection with listeriosis should not be ignored.},  
Keywords = {Listeria monocytogenes, Milk, Zoonosis, Cattle, Lorestan, PCR},
volume = {12},
Number = {2}, 
pages = {171-176}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i2.9881},
url = {http://jabs.fums.ac.ir/article-1-2787-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2787-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {KianiRad, Kowsar and Rigi, Garshasb and Reiisi, Somayeh},  
title = {Analysis of the Expression of Dopamine Receptors, DRD1 and DRD5, in Cancerous Tissue of Patients with Breast Cancer and the Impact of Quercetin on the Expression of These Receptors in Hs578t and HDF Cell Lines}, 
abstract ={Background & Objectives: The expression of neurotransmitters during cancer progression is one of the factors to consider. This study aimed to evaluate the possible expression changes in dopamine receptors (DRD1 and DRD5), in breast tumor tissue compared to healthy adjacent samples, and the effect of quercetin on the expression of these receptors. Materials & Methods: Expression levels of DRD1 and DRD5 were evaluated in 20 breast tumors and healthy adjacent samples using qPCR. The RNA was extracted from all samples, followed by cDNA synthesis and real-time PCR. In vitro experiment was accomplished on breast cancer cell line, Hs578t. Cells were treated with quercetin and evaluated cell viability by MTT assay. DRD1 and DRD5 gene expression was performed in treatment cells compared to untreated control. Statistical analysis was performed to determine the significance. Results: The results showed that the DRD1 and DRD5 were unregulated in breast cancer tissue (p<0.01). For cellular experiment, MTT assays revealed that the quercetin induced a significant decrease in cell viability and proliferation in dose and time-dependent but it was not seen in a normal cell line (HDF). Hs578t cells showed a significant reduction of DRD5 in response to quercetin. DRD1 gene downregulation was indicated significant in 72h treatment. Conclusions: The effect of quercetin on the expression of genes encoding DRD1 and DRD5 showed that this substance reduced the expression of these two genes in treated Hs578t cells compared to untreated cells in the same cell line.},  
Keywords = {Dopamine receptor, Breast cancer, Quercetin, Gene expression, Hs578t cell line, HDF cell line},
volume = {12},
Number = {2}, 
pages = {177-186}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i2.9882},
url = {http://jabs.fums.ac.ir/article-1-2750-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2750-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Morovvati, Abbas and Javadi, Ali and Dehghanisanij, Somayeh and Zarei, Mohamadreza and Molazadeh, Shima and Badakhsh, Mina and Zargar, Mohse},  
title = {Rapid Homemade Reverse Transcriptase PCR Detection and Phylogenetic Analysis for SARS-CoV-2 Based on E and M Genes}, 
abstract ={Background & Objective: Coronavirus disease 2019 spreads worldwide and needs detection systems capable of rapid diagnostic of this virus (SARS-CoV-2). The aim of this study is to design the homemade RT-PCR method for the Detection and phylogenetic analysis of this virus Material & Methods: The genes selected for diagnosis were E and M genes for this virus. PCR product was cloned in pTZ57R/T plasmid for preparation of positive control. In order to determine the sensitivity of this molecular method, the genes mentioned in the clone pTZ57R/T vector and the Limit of detection (LOD) the genes were determined and phylogenetic analysis was performed using partial E and M gene sequences. Results: PCR product was observed for E and M genes 156 and 547 bp on the Agarose gel. The LOD of the E and M gene was 60 and 82 copies. There was also a positive response to the samples of patients who were positive by other methods. Conclusions: Since this virus is considered to be the cause of a pandemic in different countries all over the world, the present study is very important as a method of rapid and low-cost molecular diagnosis for monitoring this virus. Phylogenetic analysis is necessary for epidemiological studies for the control and prevention of the disease.},  
Keywords = {RT-PCR, Covid-19, Detection, LOD, Phylogenetic Analysis },
volume = {12},
Number = {2}, 
pages = {187-193}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i2.9883},
url = {http://jabs.fums.ac.ir/article-1-2773-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2773-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Azarmi, Sareh and Hoseini‎pajooh, ‎khosro and Tajik, Parviz and Lashkari, ‎Marziyeh},  
title = {The Protective Effect of Curcumin on the Proliferation and Colonization of Spermatogonial ‎Stem Cells ‎in Gamma-Irradiated Rats}, 
abstract ={Background & Objective: One of the side effects of radiotherapy can be damage to spermatogonial stem cells that ‎may lead to spermatogenesis disorders and sterility. Protective effects of curcumin on normal cells ‎against radiotherapy side effects have already been shown. In the current study, the protective effects of curcumin ‎on the spermatogonial stem cells against gamma radiation were ‎evaluated.‎ Materials & Methods: This study was done on 50 adult rats in 10 experimental groups. Four groups were ‎injected 0, 25, 50, or 100mg/kg of curcumin in 1ml olive oil for 15 days intraperitoneally, then exposed to radiation ‎at 2 Gy on the next day. Also, four groups were treated like above but without radiation; and two groups as control with and without radiation.  The day after radiation, all of the rats were euthanatized, their testes were removed, and they underwent enzymatic digestion to co-culture spermatogonial stem cells. After 12 days, the colonization of spermatogonial stem cells was assessed. ‎ Results: There was a significant decrease in the colonization of spermatogonial stem cell proliferation ‎in groups that had taken radiation but not curcumin. There was a significant increase ‎ in the colonization of spermatogonial stem cells in the group which had taken radiation whit maximum curcumin compared with the other irradiated groups and ‎was similar to non-irradiated control animals. Colonization of spermatogonial stem cells in non-irradiated animals treated with curcumin had increased compared with control groups.‎ Conclusion: Injection of curcumin can protect spermatogonial stem cells against ‎radiation. Thus, curcumin may prevent sterility in men who undergo radiotherapy. ‎  },  
Keywords = {Curcumin, Radiation therapy, Radiation protective agents, Spermatogonial stem cells, Sterility},
volume = {12},
Number = {2}, 
pages = {194-202}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i2.9884},
url = {http://jabs.fums.ac.ir/article-1-2659-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2659-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Seratnahaei, Marjan and Eshraghi, Seyyed Saeed and Pakzad, Parviz and ZahraeiRamazani, Alireza and Yaseri, Mehdi},  
title = {Antimicrobial Activities of the Secondary Metabolite Extracted from a Nocardia Strain}, 
abstract ={Background & Objective: The emergence of antibiotic-resistant bacteria and limited treatment options for these infections is a challenging problem in the world. There is increasing interest in isolating bioactive molecules from actinobacteria and elucidating their antimicrobial mechanisms at the moment. The current study aimed to investigate the antimicrobial activities of a Nocardia strain (N4) that was previously isolated from the soil samples in Tehran, Iran.  Materials & Methods: The cultural, morphological, and physiological characteristics of the strain were determined and the identity of the strain was confirmed using 16SrRNA gene sequencing. The strain was screened for antimicrobial metabolite production. The in vitro anti-microbial activity of the extracted metabolite was assessed by agar well diffusion and minimum inhibitory concentrations methods. Finally, the structure of the extracted metabolite was confirmed by HPLC and mass spectrometry methods. Results: The isolate shared 99% sequence similarity with Nocardia soli. The strain showed broad-spectrum antimicrobial activities against some pathogenic bacteria (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 700603, Salmonella Typhimurium ATCC 14028 and Shigella sonnei RI366) and fungi (Aspergillus niger ATCC 1015 and Aspergillus fumigatus ATCC 1022) in the agar well diffusion method. The ethyl acetate extract of the strain exhibited the highest antimicrobial activity against E. coli (MIC= 4.67±3.055 μg/mL) and K. pneumoniae (MIC=3.33±1.155 μg/mL). The metabolite showed high similarity with C21H28N7O14. Conclusions: The current study confirmed the presence of a bioactive metabolite in strain N4, suggesting that this may be a promising candidate for discovering bioactive components with broad antibacterial activities against a variety of pathogens Keywords: },  
Keywords = {Nocardia soli, antimicrobial activity, HPLC, Bioactive Metabolite},
volume = {12},
Number = {2}, 
pages = {203-214}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i2.9886},
url = {http://jabs.fums.ac.ir/article-1-2700-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2700-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Chaleshi, Vahid and OloumiKalantar, Saeideh and Salahshourifar, Iman and Shahrokh, Shabnam and AsadzadehAghdaei, Hamid and Zali, Mohammad Rez},  
title = {Evaluation of LncRNA uc.173 and Occludin in Iranian Patients with Inflammatory Bowel Disease Compared with Healthy Individuals}, 
abstract ={Background & Objective: Inflammatory bowel disease (IBD) is characterized by the chronic gastrointestinal inflammation. The two common forms of IBD are ulcerative colitis (UC) and Crohn’s disease (CD) that are distinguished by their location and depth of involvement in the diffuse inflammation of the colonic mucosa and affects the rectum (proctitis). A novel class of LncRNAs transcribed from ultra-conserved regions (UCRs) is a recently identified ultra-conserved region (T-UCR) transcript that is involved in the cellular function in a variety of pathways. However, the regulation of LncRNA uc.173 in IBD remains to be fully elucidated. In this study, we aimed to examine the expression of LncRNA uc.173 and Occludin genes in an Iranian population with inflammatory bowel disease. Materials & Methods: This case-control study was performed on 33 inflammatory bowel disease patients including 13 Crohn's disease, 20 ulcerative colitis and 20 healthy controls. The mRNA levels of LncRNA uc.173 and Occludin genes were assessed using the quantitative Real-time polymerase chain reaction. The B2M was used as an internal control. The 2 -ΔΔCq method was used to determine the expression fold changes. Results: Statistically, the level of the LncRNA uc.173 gene expression between the UC and normal tissues increased significantly(P=0.0024). Also, the expression analysis revealed no significant difference between the samples of CD patients compared to the controls (P>0.05). In order to further evaluate the role of LncRNA uc.173 in IBD, the associations between the transcript levels of the LncRNA uc.173 and Occludin mRNA demonstrated significant difference in the CD tissue (R=0.59; P=0.002). In our study, the mRNA expression of Occludin gene did not show any changes in the IBD patients compared to the healthy controls. Conclusion: The increased expression of LncRNA uc.173 in the tissues of UC patients may be considered as a diagnostic or prognostic biomarker. Also, there was no correlation found between Occludin and LncRNA uc.173 expressions in the IBD patients' tissues.  },  
Keywords = {Inflammatory bowel disease, Ulcerative colitis, Crohn's disease, LncRNA uc.173, Occludin},
volume = {12},
Number = {2}, 
pages = {215-222}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i2.9888},
url = {http://jabs.fums.ac.ir/article-1-2770-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2770-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Saeed, Noor Al-Huda and Israa, Hussein Hamzah and Ghasemian, Abdolmaji},  
title = {Helianthus Annuus Seed Hydro-Alcoholic Extract Chemical Composition and Effects on Body Weight and Blood Parameters among Iraqi Patients Suffering from Type 2 Diabetes Mellitus}, 
abstract ={Background & Objectives: The purpose of the present study was to calibrate and analyze the effects of Helianthus annuus (H. annuus) seeds extract on body weight and blood parameters of type-2 diabetic-mellitus (T2DM) patients. Materials & Methods: Three-hundred cases of T2DM patients were involved in this study including 180 males and 120 females and divided into two groups (each with 150 members). The herbal extract of H. annuus with a concentration of 500mg/kg was subjected to group1 (case population), but group 2 received normal diet for three months. Gas Chromatography mass spectrometry (GC/MS) was used to analyze H. annuus compounds. Results: Major H. annuus fraction included chlorogenic acid (CGA) being 40%. The extract conferred significant decreasing effects on hypertension but not body weight and body mass index (BMI) values. The extract receiving group vs control group regarding the mean blood factors respectively included fast blood glucose (FBS) (39.2% vs 3.22%, p<0.0001), cholesterol (6.9% vs 3.22%, p=0.0237), triglyceride (5.4% vs 2.30, p=0.0113), and increase in high-density lipoprotein (HDL) (22.3% vs 5.26%, p<0.001) and low-density lipoprotein (LDL) (1% vs 2.6%, p=0.0112).   Conclusion: H. annuus major compounds included CGA (40%) contributed to the control of the hypertension, lipid profile and the blood factors among T2DM patients compared to those patients who received normal diet. More detailed investigations by participation of higher number of patients are required in this regard.  },  
Keywords = {Helianthus-annuus, Diabetes Mellitus type 2, blood factors},
volume = {12},
Number = {2}, 
pages = {223-228}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i2.9890},
url = {http://jabs.fums.ac.ir/article-1-2793-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2793-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Ashrafi, Mojtaba and Afsharimoghadam, Amin and Rezaiekeikhaie, Khadije and Kamali, Atefeh},  
title = {The Report of a Surgical Residual Langas Inside the Renal Pelvis 16 Years after Surgery “Case Report”}, 
abstract ={Background & Objective: Gossypiboma is the meaning of a residual foreign body such as gas in the abdomen following surgical operation, which is a rare complication of surgery. Residual of a foreign body causes mass, fistula, abscess and, obstruction after surgical operation. Computed tomography (CT) scan is a very useful method in the diagnosis of Gossipiboma. In patients who referred with abdominal pain and unexplained manifestations after surgery, Gossypiboma should be considered. Case presentation: A retrospective case study was performed in the year 2020 in a patient with a history of open kidney stone surgery in Hospital of Zabol. A 60-year-old male patient who referred with complaint of neck pain to an orthopedic doctor, which due to a history of kidney stone, the doctor orders an ultrasound of the kidneys. In the ultrasound, a number of stones are observed in the right kidney of this patient. After performing CT scan and Intravenous pyelography (IVP), a foreign body is identified inside the renal pelvis. In the following, the history taken from the patient, the history of open kidney stone surgery during 16 years ago in this patient is determined. During this period, the patient did not have any symptoms, including infection or pain in the mentioned area. Conclusion: In patients with a history of surgery who are referred with abdominal pain or mass, the residual foreign body should be included in the list of differential diagnoses.  },  
Keywords = {Gossipiboma, Holder surgical sponges, Kidney stone},
volume = {12},
Number = {3}, 
pages = {229-234}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i3.10707},
url = {http://jabs.fums.ac.ir/article-1-2806-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2806-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Modaresi, Farzaneh and Eslamzadeh, Mahboubeh and Karimi, Aliasghar and Ansari, Amir},  
title = {Early Diagnosis of Schizophrenia and Secondary Diagnose of Cerebral autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy: A Case Report}, 
abstract ={Background & Objective: Cerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy (CADASIL) is a rare disease with a prevalence estimation of, 5 per, 100,000 individuals. Besides, it can highly be misdiagnosed with other psychiatric disorders. Case Presentation: We report a case of CADASIL in a 32-year-old Asian female presenting with schizophrenia symptoms such as auditory hallucination ،loosening of associations، persecutory delusions، referential delusions، decreased function, and complex hepatic hallucinations that the patient had said sometimes someone has intercourse with me since 2 years ago. After brain Magnetic Resonance Imaging (MRI) and gene checking, she showed up with NOTCH3 gene, besides Brain Magnetic resonance imaging confirmed the presence of CADASIL. Supportive treatments have been done and two years of following up showed a significant increase in patient life quality. Conclusion: This case report demonstrates the challenges of CADASIL diagnosis in a patient with schizophrenia symptoms. The diagnosis should be considered in patients with schizophrenia symptoms. Hence, there is a need for timely diagnosis and management of this disorder. Keywords: CADASIL, Brain Magnetic resonance imaging, NOTCH3 Gene, schizophrenia},  
Keywords = {CADASIL, Brain Magnetic resonance imaging, NOTCH3 Gene, schizophrenia},
volume = {12},
Number = {3}, 
pages = {235-239}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i3.10708},
url = {http://jabs.fums.ac.ir/article-1-2826-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2826-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Ghasemian, Abdolmajid and Mubarak, Shaden MH and Memarian, Mojtaba and Memarian, Hamed and Falsafi, Sarvenaz and Zahra, Najafiolya and SeyyedKhalil, Shokouhi Mostafavi},  
title = {Recent Emergence and Re-Emergence of Crimean Congo Hemorrhagic Fever and Q Fever Zoonotic Diseases: Major yet Ignored Infectious Diseases Worldwide}, 
abstract ={The continuing emergence or re-emergence of vector-borne zoonotic Q fever (caused by Coxiella burnetii) and Crimean Congo hemorrhagic fever (CCHF, caused by Orthonairovirus) include indispensable extraordinary threat around the world. Low infectious dose and long-term environmental residence are major risks. Wildlife and domestic livestock act as hosts or reservoirs of the CCHF virus and ticks are carriers. The disease also poses a threat to public health services owing to its epidemic potential, high case fatality ratio (up to 40%) as well as difficulties in treatment, prevention, and control. Q fever is another zoonotic febrile disease mainly affecting workers involved in farming livestock. The causative agent of Q fever causes abortion in livestock. The pathogen is shed in large numbers in the waste of infected animals (amniotic fluids and placenta during parturition) and is transmitted by inhalation of contaminated aerosols. Vaccination is the most effective way of protecting against Q fever. The main way to prevent Q fever is to avoid contact with animals, especially while animals are giving birth, or consumption of unpasteurized milk and contaminated dairy products. Due to the increasing importation of livestock to meet the growing demand for dairy and meat products, new diseases are likely to be introduced. In our growing globalized world, where trade between countries increases, it is necessary to conduct more research on zoonotic diseases and to monitor any possible disease introduction to new areas. A continuing surveillance program and pathogen testing are important in tracking the emergence of new pathogens.  },  
Keywords = {Zoonoses, Crimean Congo hemorrhagic fever, Q fever, Risk factors},
volume = {12},
Number = {3}, 
pages = {240-249}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i3.10709},
url = {http://jabs.fums.ac.ir/article-1-2799-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2799-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Rezaei, Maryam and Yousefizadeh, Shahnaz},  
title = {Effects of Buprenorphine on Liver Enzymes in Patients without a History of Liver Disease}, 
abstract ={Background & Objective: Buprenorphine (BUP), a synthetic opioid, treats pain and opioid use syndrome. The potential of BUP to cause liver toxicity has not been fully evaluated. The present literature review was designed to investigate the impact of BUP treatment on liver function in patients without a previous history of liver diseases. Materials & Methods: A literature review was implemented within databases of Scopus, PubMed, ISI, and Cochran until February 2022. Studies published in English were included in this study. Retrieved citations were screened and data were extracted by at least two independent reviewers. Results: Of the 1853 studies screened citations, 14 research reports were eligible. Overall, among the randomized controlled trial, four studies reported hepatotoxicity in patients who had a history of hepatitis C or hepatitis B seroconversion under BUP treatment. Conclusion: No strong evidence was found for hepatotoxicity of BUP in this study. Elevation in the liver enzyme levels in some patients may be related to other factors such as infectious diseases, illicit drugs, alcohol consumption, environmental pollutants, and chronic diseases. More experimental and clinical studies should be conducted to address this question.  },  
Keywords = {Buprenorphine, Liver, Toxicity, Hepatitis},
volume = {12},
Number = {3}, 
pages = {250-263}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i3.10710},
url = {http://jabs.fums.ac.ir/article-1-2762-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2762-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Hojjati, Sarah and Vojdani, Salman and Hosseini, Seyyed Mohammad Ami},  
title = {The Relationship between Physical Activity during Pregnancy, Post-Cesarean-Section Pain, and Analgesia Requirement}, 
abstract ={Background & Objective: The cesarean section increases worldwide and has many side effects, including acute pain. This study investigated the relationship between physical activity during pregnancy, analgesic consumption, and maximal postoperative pain in women with low segment cesarean section. Materials & Methods: 340 Cesarean section women were interviewed by demographic and global physical activity questionnaires during the pre-operative visits. The participants were categorized into high, moderate, and low physical activity groups (high PA, moderate PA, low PA, respectively) according to the global physical activity questionnaire guidelines. The maximal postoperative pain (MPP), the type, and doses of analgesia used/2 days were recorded. Pearson correlation, Chi-square, and one-way ANOVA were used to analyze the data. Results: MPP was reduced in the high PA group (5.48 ±1.72) compared to the moderate (6.46±1.30) and low PA groups (6.97±1.92; p<0.0005, p<0.0005, respectively). There was a difference between the moderate and low PA groups (p=0.04). Paracetamol was the common analgesic without significant difference among groups (p=0.37). The numbers of paracetamol doses significantly reduced in the high PA group (3.31±1.65) compared to the low PA group (4.03±2.01, p=0.01). MPP had a significant and low negative correlation with total physical activity (r=-0.25, p=0.0005). There was a negative significant correlation between occupation (r=-.491, p=0.0005), recreational (r=-.262, p=0.0005), and travel activities (r=-.150. p=0.006) with MPP. There was a low positive correlation between sedentary activity and MPP (r=0.23, p=0.0005).  Conclusions: Maternal physical activity can be a non-pharmacological and cost-effective method of pain management.  },  
Keywords = {Acute Pain, Physical Activity, Pregnant Women},
volume = {12},
Number = {3}, 
pages = {264-274}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i3.10711},
url = {http://jabs.fums.ac.ir/article-1-2812-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2812-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Modaresi, Farzaneh and Ansari, Amir and Pezeshki, Babak and Parou, Zahra and Karimi, Aliasghar and Eslamzadeh, Mahboubeh and Ahrari, Fatemeh},  
title = {Depression among Adults with Diabetes in Fasa Diabetes Registry System: Risk Factors and Relationship to Blood Sugar Control}, 
abstract ={Background & Objective: Diabetes Mellitus is a chronic disorder that affects patients physically and mentally. It is a co-existing disorder that can impact disease management adversely and trigger diabetes-related complications. However, it seems that the role of factors associated with depression are underestimated by both patients and physicians. We examined the associations between depression and blood sugar control and the role of associated factors such as demographic features, Glycated hemoglobin(HbA1c), Fasting Blood Sugar, lifestyle and diabetes-related complications on depression in adults with diabetes mellitus. Materials & Methods: We conducted a cross-sectional study among 219 adults diagnosed with diabetes. Participants completed a questionnaire measuring depressive symptoms and demographic backgrounds. Laboratory values and data from physical examination were also collected. Results: The prevalence of depression was 12.3% in our population. The mean level of HbA1c and FBS was also higher among depressed patients. however, it was not significantly different among patients with and without depression. There was no statistically significant correlation between the Beck Depression Scale mean scores and the mean of FBS, HbA1c and BMI (p<0.01). Conclusion: Our findings reveal that depression rate among diabetes patients is considerable. In fact, mental status should be noticed as well as pathological status in clinical practice.  },  
Keywords = {Depression, Diabetes, Blood Glucose control},
volume = {12},
Number = {3}, 
pages = {275-280}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i3.10712},
url = {http://jabs.fums.ac.ir/article-1-2823-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2823-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Hosseini, Shima and Rahimi, Maryam and Farmoudeh, Ali and FallahAmoli, Amirali and BeheshtiMonfared, Niloufar},  
title = {Effects of Magnesium-Vitamin E Co-supplementation on Glucose Metabolism, Oxidative Stress, and Pregnancy Outcomes Among Women with Gestational Diabetes: A Double-blind Clinical Trial Study}, 
abstract ={Background & Objective: Dietary antioxidants may play a protective role in the pathophysiology of gestational diabetes mellitus (GDM). The present study aimed to evaluate the effect of magnesium-vitamin E co-supplementation on glycemic control and pregnancy outcomes in women with GDM. Materials & Methods: This randomized clinical trial was conducted among GDM pregnant women at 24-28 weeks of gestation. The study did not include mothers who needed medication to control their blood glucose levels or had a history of DM. The subjects were randomly divided into two 30-member groups, one of which received magnesium (250 mg), and vitamin E (400 mg) daily, while the other was given a placebo. Blood samples were taken at the study baseline and six weeks after the intervention to quantify oxidative stress biomarkers, fasting plasma glucose (FPG), and fasting plasma insulin (FPI). Additionally, the effect of supplementation was assessed on neonatal outcomes. Data analysis was performed using SPSS 20 (SPSS, Chicago, IL) Results: A significant decrease was observed in the FPG and FPI of the supplementation group (p<0.05). However, FPI elevated by progressing pregnancy in the placebo group (p=1.99×10-4). The homeostasis model assessment (HOMA) revealed that supplementation was associated with improved insulin sensitivity (p=3.38×10-13). In addition, total antioxidant capacity increased to 5.66 ± 0.86% in the treatment group. Finally, no significant difference was found between the groups regarding neonatal outcomes. Conclusion: The results represented that magnesium-vitamin E co-supplementation significantly reduced oxidative biomarkers and improved glycemic control in GDM.  },  
Keywords = {Magnesium, Vitamin E, Pregnancy,  Diabetes Mellitus, Oxidative stress},
volume = {12},
Number = {3}, 
pages = {281-291}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i3.10713},
url = {http://jabs.fums.ac.ir/article-1-2765-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2765-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {SorayabinMobarhan, Fatemeh and Teimouri, Maryam and Pooladi, Mehdi},  
title = {Overcoming Drug Resistance in Breast Cancer Using Quercetin Nanodrugs}, 
abstract ={Background & Objective: Breast cancer is one of the most common cancers in the world. Treatment methods for this disease have been with poor results. In recent years, more attention has been paid to stem cells due to their cytotoxic properties. According to previous results, the effect of nanoquercetin on breast cancer cells in this study was used concomitantly with doxorubicin, which is a chemotherapy drug and in some patients has therapeutic resistance. Materials & Methods: The above study was performed on MCF7 and BT474 human breast cancer cells. Quercetin was synthesized, then different concentrations of 10, 20, 40, 80 and 160 of quercetin and 1, 2.5, 5 and 10 μg/ml of doxorubicin were prepared and two cell lines were treated for 24 h. MTT test was used to determine cell viability and flow cytometry test was used to determine the extent of apoptosis in both cell lines. Results: The results showed that MCF7 and BT474 cells that were affected by quercetin and doxorubicin, increasing the concentration of quercetin and doxorubicin decreased the survival of both cell lines within 24 h. The LC50 concentration was calculated for both cells treated with nanoquercetin 40 μg/ml and for doxorubicin doxorubicin (0.1 mg/ml). The results also showed that in both cell lines, an increase in cellular apoptosis occurred after incubation with quercetin and then incubation with doxorubicin. Conclusion: Comparison of doxorubicin (DOX), quercetin (QU) and quercetin and doxorubicin (QU-DOX) groups with controls shows that with the presence of quercetin-doxorubicin, it had a greater inhibitory effect on cancer cells.  },  
Keywords = {Quercetin, Doxorubicin, MCF-7, BT474, Breast Cancer},
volume = {12},
Number = {3}, 
pages = {292-301}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i3.10714},
url = {http://jabs.fums.ac.ir/article-1-2761-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2761-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Modaresi, Farzaneh and Eslamzadeh, Mahboubeh and RezaeiMotlagh, Mohammad Ali and Pezeshki, Babak and Karimi, Aliasghar and Ansari, Amir},  
title = {Anxiety among Adults with Diabetes in Fasa Diabetes Registry System: Risk Factors and Relationship to Blood Sugar Control}, 
abstract ={Background & Objective: Diabetes mellitus (DM) became a critical worldwide issue in last few decades. One of the progressive concerns is mental health in diabetic patients. Anxiety is one of mental health disorders that is comprehensively prevalent in diabetic patients. The investigation of anxiety in diabetes and poor glycemic control and other factors is related to comorbid anxiety with diabetes. Materials & Methods: 220 diabetic patients who were registered on Fasa diabetes registry system. We interviewed them on telephone and collected Persian version of Beck anxiety inventory (BAI). Result: Our study showed that prevalence of anxiety in diabetic patients was 29.5%. Among this the majority of them were females, i.e. 148 patients (67.3 %) and 72 (32.7%) were males and mean age was 57.16 years. Among all factors, glycemic control (glycosylated hemoglobin type A1C (HbA1C) and FBS) were not significantly related to anxiety in diabetes subjects. However significant relation between demographic factors (sex, having child and job) was detected. Conclusion: Our study revealed a high prevalence of anxiety among diabetic patients and shows a significant relation with factors like sex, occupation, and job satisfaction. On the other hand, no significant relation between glycemic control and anxiety was observed. However, longitudinal study needs to identify detailed risk factors.  },  
Keywords = {diabetes, anxiety, diabetes mellitus, glycosylated hemoglobin type A1C },
volume = {12},
Number = {3}, 
pages = {302-310}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i3.10715},
url = {http://jabs.fums.ac.ir/article-1-2824-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2824-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Ghahremani, Nasrin and Mahmoudi, Farib},  
title = {Effects of Thymoquinone on Hypothalamic NPY and AgRP Gene Expression in Intact and Hypothyroidism- Model Rats}, 
abstract ={Background & Objective: Thymoquinone is the most important compound of Nigella sativa, which stimulates the activity of thyroid axis in healthy individuals and people with hypothyroidism. The present study investigated the effects of thymoquinone on Neuropeptide Y (NPY) and Agouti-dependent peptide (AgRP) gene expression in the hypothalamus of healthy individuals and hypothyroidism- model rats Material & Methods: Twenty male Wistar rats weighing 190- 220g were used. Hypothyroidism was induced by a daily consumption of Methimazole (20mg/kg) for 42 days via drinking water. Control rats received the intraperitoneal injections of saline. Intact or hypothyroid rats received the intraperitoneal injections of thymoquinone (10mg/kg) for 15 days. One day after last injection, the thyroid gland and hypothalamic samples were dissected. Thyroid gland samples were used for histological study. Relative gene expression of hypothalamic NPY and AgRP was determined by real-time polymerase chain reaction. Results: Thymoquinone significantly declined the NPY and AgRP gene expressions in the hypothalamus of intact rats in comparison with control group. Induction of hypothyroidism results in a remarkable increase in the NPY and AgRP gene expressions compared to control rats. In hypothyroid rats receiving thymoquinone, the mean relative NPY and AgRP gene expressions showed an insignificant decrease compared to hypothyroid group. Conclusion: Because AgRP/NPY signaling pathway exerts inhibitory effects on thyroid gland function, thymoquinone may stimulate thyroid axis activity partly via inhibiting the hypothalamic AgRP/NPY gene expression in intact rats. In hypothyroidism, used dose of thymoquinone may not able to cause a significant decrease in AgRP/NPY gene expression due to its increased levels.  },  
Keywords = {Thymoquinone, Black Seed, Hypothyroidism, AgRP, NPY, Methimazole},
volume = {12},
Number = {3}, 
pages = {311-318}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i3.10716},
url = {http://jabs.fums.ac.ir/article-1-2837-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2837-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Honarmand, Saeideh and Montaseri, Zahra and Sharafi, Mehdi and Najafipoor, Sohrab},  
title = {A Comparison between the Efficiency of Vonavir (Tenofovir-Emtricitabine- Efavirenz) and Cobavir (Lamivudine-Zidovudine) with Efavirenz used for HIV Patients in Fasa, Iran}, 
abstract ={Background & Objective: Many different combination regimens have been used for the treatment of patients infected with human immunodeficiency viruses (HIV). This study aimed to compare the efficacy of two antiviral drugs for the treatment of HIV-infected patients. Materials & Methods: This cross-sectional study was performed on HIV-positive patients in Fasa, Southwest Iran. Eighty patients were enrolled in the investigation who were then randomly divided into two groups and treated plus vonavir (tenofovir-emtricitabine-efavirenz) and cobavir (lamivudine-zidovudine) with efavirenz for six months. Blood samples collected from all patients were examined for viral load every six months using Real-time PCR and CD4 changes by flow cytometry. Result: During the six months of treatment, the CD4 response was not significantly increased in group one, treated with vonavir. In contrast, the CD4 value showed a significant increasing trend during the treatment course in group two treated with cobavir-efavirenz (P=0.003). However, overall, there was no statistically significant difference between the CD4 responses of the two groups (P=0.361). In addition, the plasma viral load was significantly suppressed in both regimens (P< 0.05). Conclusion: Hence, the two regimens (cobavir-efavirenz, and vobavir) showed the same efficacy on HIV patients according to the same suppression of viral load, and CD4 response in this region. However, inclusion of more samples is needed and more studies are suggested in order to confirm our results as well.  },  
Keywords = {Tenofovir-Emtricitabine-Efavirenz, Lamivudine-Zidovudine, HIV, CD4, Viral load, Iran},
volume = {12},
Number = {3}, 
pages = {319-324}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i3.10717},
url = {http://jabs.fums.ac.ir/article-1-2839-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2839-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {SheykhzadeAsadi, Maryam and Kheirkhah, Babak and Bahador, Nim},  
title = {Determination of Type and Molecular Identity of Clostridium perfringens Isolated from Patients with Multiple Sclerosis (MS)}, 
abstract ={Background & Objectives: Multiple sclerosis (MS) is a chronic inflammatory autoimmune disease of the central nervous system (CNS). Clostridium perfringens ε-toxin (ETX) can cause emerging complications of MS due to its tendency to the blood-brain barrier. This study aimed to determine the presence of toxin-producing genes in intestinal C.perfringens in patients with multiple sclerosis. Materials & Methods: Stool samples were taken from 70 MS patients and 70 without MS individuals. The samples were enriched in cooked meat media, and cultural and biochemical methods separated Clostridium isolates. PCR tested these isolates to identify C. perfringens species. In addition, the presence of alpha, beta, epsilon and iota toxin-producing genes was evaluated in all isolates by Multiplex PCR. Results: Isolates containing the etx gene were observed in 10 patients, while no itxA gene was identified in any isolates. The results showed that isolates in 8 patients were type D. Also, the gene encoding toxin type D was identified in 2 isolates obtained from the control group. Conclusion: Our findings indicated the high frequency of C. Perfringens in MS patients. In the studied samples with clinical presentations, most of these organisms were type D bacteria that produce ε-toxin.},  
Keywords = {Clostridium perfringence, ε-toxin, multiple sclerosis, toxin-producing genes},
volume = {12},
Number = {3}, 
pages = {325-335}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i3.10718},
url = {http://jabs.fums.ac.ir/article-1-2791-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2791-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Kargar, Mansour and Fardid, Reza and Farhadi, Ali},  
title = {Muc-1 Family Tumor Markers and Their Role in the Diagnosis of Breast Cancer, Review Article}, 
abstract ={Background & Objective: Tumor markers are elements produced by tumors or other cells in the body in response to cancer or some benign condition. Although most of these markers are made by normal cells as well as cancer cells, they are produced at much higher levels in cancerous conditions. This study aimed to provide a method for using tumor markers to diagnose cancer and detect the presence of metastasis and recurrence of the disease. Materials & Methods: The present narrative review study was done by selecting the appropriate keywords and searching for research and review studies indexed in Google Scholar, PubMed, Science Direct, and SID databases. Results: These studies often indicate the effective role of tumor markers in the MUC-1 family (especially Cancer antigen15-3(CA15-3)) and CEA (carcinoembryonic antigen) as the most widely used tumor markers in patients with breast cancer. Conclusions: Based on information from studies on tumor markers, the combination of CEA, CA15-3, PRL (prolactin), KL-6(Kerbs von den Lungen), Thioredoxin 1, and FER (ferritin) tumor markers can increase the sensitivity of early-stage breast cancer detection, and CA15-3 tumor markers can also be used to identify the presence or absence of metastasis to the axillary lymph nodes. The use of ultrasound (especially color Doppler) and its combination with CEA and CA15-3 tumor markers are recommended to improve the accuracy of a breast cancer diagnosis.},  
Keywords = {CA15-3 protein, Carcinoembryonic Antigen, MUC1 protein},
volume = {12},
Number = {4}, 
pages = {336-345}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i4.11435},
url = {http://jabs.fums.ac.ir/article-1-2841-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2841-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Sadeghzadeh, Azizeh and Sadeghzadeh, Firouzeh and Mahmoudi, Farib},  
title = {The Effects of COVID-19/ACE2 on Pregnancy Events and Outcomes:  A Systematic Review}, 
abstract ={Background & Objectives: Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) was confirmed as the cause of Coronavirus disease 2019 (COVID-19). The disease presents with a wide range of clinical signs and symptoms involving vital organs such as the lungs, heart, gastrointestinal tract, liver, central nervous system, blood, and kidneys. It also potentially affects other organs, including the placenta.The present systematic review aimed to evaluate effects of SARS-CoV-2 on pregnant women, fetuses, and infants born to infected mothers. Material & Methods: The search fields used in this study were angiotensin-converting enzyme receptor (ACE2), fetus, pregnancy, and SARS-CoV-2. We reviewed articles published in 2020 and 2022. The inclusion criteria were articles on receptor expression, virus entry into the host cell, studies on the characteristics and outcomes of pregnant or recently pregnant women with SARS-CoV-2 infection who were approved and characteristics and outcomes of infants whose mothers were infected with SARS-CoV-2. Results: The virus uses the ACE2 to enter the cell. The coronavirus can be expected to affect any cell or organ that expresses ACE2. Female reproductive system is one of the systems that express ACE2. The destructive effects of COVID-19 on maternal and fetal health are strongly influenced by the spatial-temporal distribution of ACE2. Conclusion: The harmful role of COVID-19 in pregnancy is highly controversial, although maternal COVID-19 infection contributes to adverse consequences of pregnancy. There is a limited amount of information on the efficacy of COVID-19 on pregnants and their fetuses.},  
Keywords = {COVID-19, Pregnant women, Fetus, ACE-2, SARS-CoV-2, Neonate},
volume = {12},
Number = {4}, 
pages = {346-354}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i4.11436},
url = {http://jabs.fums.ac.ir/article-1-2786-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2786-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Agharezaee, Niloofar and Forouzesh, Flor},  
title = {Comparing Genetic Information of Severe Acute Respiratory Syndrome Corona Virus 2 and SARS CoV and Middle East Respiratory Syndrome Corona Viruses; A Review Study}, 
abstract ={Background & Objective: The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to a global infection and a continuing pandemic. Symptoms have been associated with age, biological sex, and other previous present health situations. The aim of this study was to make a genetic comparison between the SARS-CoV-2, SARS-CoV, and Middle East Respiratory Syndrome (MERS)-CoV using phylogenetic methods. Materials & Methods: In this review study, previously published papers were collected from 2010 to 2020 using electronic sources in reliable databases such as Scopus, PubMed, ISI, Google Scholar, and Science Direct. Then, we performed the bioinformatics study. For sequences alignment, analysis, and phylogenetic construction, all sequences from SARS-CoV-2, SARS-CoV, and MERS-CoV were obtained from the GeneBank database. Results: Eighty percent of SARS-CoV and 50% of MERS-CoV RNA sequences shared with SARS-CoV-2 RNA and SARS-CoV-2 show additional genomic regions. The phylogenetic analysis of the full-length genome sequence shows that SARS-CoV-2 has the highest similarity with SARS-CoV, but it has a lower similarity with MERS-CoV. Considering the close relationship between SARS-2 and SARS, the examination of the amino-corrosive translocation of completely different proteins may prove that there are fundamental and utilitarian differences between SARS-2 and SARS. Conclusion: Although COVID-19 pathogenicity is not well known, MERS-CoV and SARS-CoV pathogenesis still can be the best source of COVID-19 information. The global effect of this new epidemic is yet uncertain. So, analyzing genome sequencing is important for epidemiological, clinical, and experimental studies.},  
Keywords = {Coronavirus genome, MERS-CoV, SARS-CoV, SARS-CoV-2, Phylogenic tree, COVID-19},
volume = {12},
Number = {4}, 
pages = {355-366}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i4.11437},
url = {http://jabs.fums.ac.ir/article-1-2827-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2827-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Ghaforyan, Hossein and Mohammadibilankohi, Sara and Khalilpour, Habib},  
title = {Optimization of Ion Energy for the Treatment of Cancerous Tumors}, 
abstract ={Background & Objectives: For over 60 years, proton beams and heavy ions have been a powerful ion therapy method for treating cancerous tumors. It is an inherent ability of high-energy ions to discharge their energy at a certain depth with high doses, which is impossible for other beams such as X-rays, gamma rays, and electron beams. Moreover, it is an excellent way to protect healthy tissues in ion therapy. Materials & Methods: Scientists have used radio frequency (RF) accelerators to generate high-energy ions. However, due to the high price and large devices, laser-plasma accelerators have received much attention. This paper has studied the best conditions for producing high-energy argon ions. For this purpose, the interaction of high-intensity laser pulses with argon nanoclusters is simulated. This simulation is based on the nanoplasma model by the particle in cell method (PIC). Results: The simulation results show that the ion energy is dependent on the parameters of the irradiated laser and the parameters of the target cluster. Conclusions: The energy of the ions increases with the intensity of the laser and the duration of the laser pulse. Access to higher energy ions is also possible by changing the radius of the cluster. The density of the initial atoms of the cluster is also an important parameter that influences the energy of ions.},  
Keywords = {laser-cluster interaction, accelerated ions, nanoplasma model, ion therapy, particle in cell method},
volume = {12},
Number = {4}, 
pages = {367-377}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i4.11438},
url = {http://jabs.fums.ac.ir/article-1-2848-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2848-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Foruzandeh, Zahra and Alivand, Mohammad Reza and GhiamiRad, Mehdi and Zaefizadeh, Mohammad and Ghorbian, Saei},  
title = {Hsa-miR-662 as a New Prognostic Biomarker in Patients with Breast Cancer; In-silico and Experimental Study}, 
abstract ={Background & Objective: Breast cancer (BC) is a complex genetic disease that has an average annual incidence of one million people and is the second leading cause of death among women in the world. Therefore, a better understanding of tumor biology and the determination of biomarkers for early diagnosis of disease is essential. MicroRNAs and long non-coding RNAs are novel gene regulators that play key roles in tumor initiation and progression. The current study was performed to assess the biomarker potential. This study performed a combination of in-silico and experimental investigations of altered miRNAs in BC to assess the use of miRNAs as novel biomarkers for early detection and prognosis prediction of patients with BC. Materials & Methods: We searched the miRNA expression patterns of BC from three expression arrays (GSE58606, GSE38867, and GSE40525,) from the Gene Expression Omnibus (GEO) database to recognize differentially expressed miRNAs (DEMs) between BC tissues and normal adjacent samples. Using “Limma" package's Quantile Normalization function and INMEX bioinformatic tool, hub DEMs were identified. MiRNAs targeted genes were found and visualized via the miRWalk and miRTargetLink databases and their Enrichment analysis was performed for identified genes. Due to more validation of DEGs, GSE70951, an independent expression array dataset, was analyzed. By merging DEMs and DEGs, miRNA-mRNA network was constructed. After elucidation of hub miRNAs, the capacity of detected miRNAs to differentiate BC from adjacent controls was estimated by Kaplan-Meier analysis.  Furthermore, RT-qPCR on 100 BC samples and 100 adjacent non-tumor tissues was performed to validate the in-silico results. Results: According to our study, in BC samples, miR-662 was differentially downregulated in comparison with normal adjacent tissues. Conclusion: Altogether, miR-662 can be considered as a viable target for BC diagnostics and treatment.},  
Keywords = {Breast Cancer, MicroRNA, Hsa-miR-662, Prognosis},
volume = {12},
Number = {4}, 
pages = {378-387}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i4.11439},
url = {http://jabs.fums.ac.ir/article-1-2883-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2883-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Abdollahi, Abbas and Montaseri, Zahra and Yazdanparast, Seyed Saeid and Montaseri, Maryam},  
title = {Colonization and Antibiotic Resistance of Nasal Staphylococcus Aureus among Healthcare Workers in Southwestern Iran: Occurrence of OS-MRSA}, 
abstract ={Background & Objectives: Staphylococcus spp. is a resident flora of the skin and mucosa of humans that can colonize the anterior nares of individuals. This cross-sectional study was conducted to determine the rate and antibiotic resistance pattern of nasal Staphylococcus aureus (S. aureus) carriers among the staff of Fasa hospital, southern Iran. Materials & Methods: Nasal swab samples were collected from 117 hospital staff working in 12 wards. Microbiological culture method was applied for S. aureus identification. The isolates were confirmed by tuf gene identification using PCR assay. Five isolates were randomly sequenced and phylogenetically analysed  using MEGA software. The antimicrobial resistance pattern of the isolates was evaluated using the disc diffusion assay and the amplification of the methicillin resistance (mecA) gene. Results: The prevalence of S. aureus nasal carriers included 10.26% (n=12). The nasal carriers were identified in the wards of surgery ICU, gynecologic surgery, NICU, pediatric, internal surgery, and emergency. Among them, gynecologic surgery staff had the highest rate of nasal colonization (33.33%). Phylogenetic analysis showed that of five isolates, four had high similarities with each other. Also, the highest rate of resistance was related to penicillin (83.3%), followed by cefazolin (75%), and cephalexin (75%). However, the highest level of susceptibility (100%) was found for vancomycin, cefoxitin, and oxacillin. Furthermore, the methicillin resistance gene (mecA) was highly detected (75%) from the isolates, elucidating oxacillin-susceptible or cefoxitin-susceptible mecA-positive S. aureus (OS-MRSA). Conclusions: The high rates of OS-MRSA can lead to antibiotic resistance among health care workers tremendously. Moreover, the high similarity probability in phylogenetic analysis shows the possibility of cross-infection between these health care workers, warning to exert effective strategies to control infection spread, especially in the surgery ward.},  
Keywords = {Staphylococcus aureus, OS-MRSA, MecA gene, Healthcare workers,Colonization},
volume = {12},
Number = {4}, 
pages = {388-396}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i4.11440},
url = {http://jabs.fums.ac.ir/article-1-2863-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2863-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {RezaieKeikhaie, Khadije and RezaieKahkhaie, leli and Saravani, Khadijeh and Shahraki, Zahra and Ghaffari, Hamid Reza and BadiBaigan, Saeed and Ahmadzadeh, Mozhgan and NakhaeeMoghadam, Maryam and kamali, Atefeh},  
title = {The Effect of Intravenous and Oral Fluid Therapy on Women with Third Trimester Oligohydramnios of Pregnancy and the Fetus}, 
abstract ={Background & Objective: Adequate amniotic fluid volume is one of the most important aspects of fetal health and Oligohydramnios refers to amniotic fluid volume that is less than expected for gestational age. The aim of this study was to compare the trend of amniotic fluid changes in the two groups of oral and injectable before and after fluid therapy. Materials & Methods: In the present study, pregnant women with oligohydramnios in the third trimester referring to Amir Al-Momenin Hospital (Zabol, 2019) were randomly divided into two groups of case. The first group received oral water and the second group was treated with serum therapy. Eventually, all patients underwent ultrasonography and their AFI was recalculated and compared. Data was analyzed using SPSS 20 statistical software. Results: The results of the study showed that there was no statistically significant difference between the amniotic fluid index before fluid therapy in the oral and injectable (serum therapy) groups (p = 0.426). After fluid therapy, the amniotic fluid index in the oral group was significantly higher than the injected group (p <0.001). Although in both oral and injectable groups, the amniotic fluid index increased significantly after treatment (p <0.001), the increase in the oral group was significantly higher than the injectable group. Conclusion: The results of this study showed that oral and intravenous fluid therapy improves the amniotic fluid index more than injectable fluid therapy.  },  
Keywords = {Amniotic Fluid Index, Intravenous Hydration, Isolated Oligohydramnios, Oral Hydration},
volume = {12},
Number = {4}, 
pages = {397-405}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i4.11441},
url = {http://jabs.fums.ac.ir/article-1-2802-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2802-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {H.Alsaadi, Zainab and KadlimHindi, Nada and AlMarzoqi, Ali H. and Memariani, Mojtaba and Kohansal, Maryam and Ghasemian, Abdolmaji},  
title = {The Association of cagA, vacA, babA2, babB and oipA of Helicobacter pylori with Risk of Gastric Carcinoma Development}, 
abstract ={Background & Objective: Helicobacter pylori (H. pylori), carried by more than half of the world population, is a major cause of chronic duodenal and gastric ulcers, gastritis and carcinoma. Colonization and toxin production include major virulence traits of H. pylori. The aim of this study was to assess the existence of H. pylori and virulence factors among patients with risk of gastrointestinal carcinoma (GC) in an Iraqi population. Materials & Methods: During May 2016- October 2020 in Babylon, Iraq, a total of 500 biopsy samples were obtained from gastric tissue of patients with GC, gastritis, duodenitis, duodenal ulcer and gastric ulcer and cultured onto the Brucella agar. H. pylori isolates were identified using conventional biochemical and molecular tests. Molecular identification was conducted by amplification of glmM gene using the polymerase chain reaction (PCR) technique. The adhesin (babA2, babB and oipA) and toxin (cagA and vacA) genes were also amplified using PCR technique. Results: Among 500 biopsy samples, 269 (110 from males and 159 from female patients) H. pylori isolates were identified. The age range of patients was 14-69 years (mean age=47.34±7.23). The babA2 and babB genes were detected in 59.47% and 59.10% of isolates, respectively. Notably, babA2 was observed in 89% of GC and 64% of DN strains being significantly more associated with GC and DN (<0.0001 and 0.028, respectively). Furthermore, babB-positive strains were significantly (0.042) more associated with PG. The rate of cagA and vacA was 44.60% and 48.32%, respectively. The cagA was detected in 64.73% of GC, and 100% of PG and DN strains with a significant association. We detected the oipA in 58.36% of strains which was significantly associated with GC (74%, P=0.0001), PG (88%, p<0.0001) and DN (84%, p<0.0001) as compared to oipA-negative strains. Conclusion: The existence of H. pylori babA2, cagA and oipA virulence genes was associated with GC, DN and PG. As these genes play a crucial role in the development of gastric carcinoma, accurate control measure toward hindering the colonization of pathogenic strains is essential.},  
Keywords = {Helicobacter pylori, colonization, virulence, gastric carcinoma, duodenal cancer},
volume = {12},
Number = {4}, 
pages = {406-411}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i4.11442},
url = {http://jabs.fums.ac.ir/article-1-2784-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2784-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Ghazaei, Ciamak},  
title = {Pseudomonas aeruginosa: Prevalence of Pathogenic Genes, OprL and ToxA in Human and Veterinary Clinical Samples in Ardabil, Iran, 2020}, 
abstract ={Background & Objective: An opportunistic pathogen Pseudomonas aeruginosa can cause frequent hospital-acquired infections as well as one microorganism in the food spoilage. Also, the emergence of multidrug-resistant P. aeruginosa has become a serious threat to public health.This pathogen has many virulence factors which aid in bacterial invasion as well as toxicity, during infections. Out of different virulence genes in Pseudomonas aeruginosa, oprL (Encoding membrane lipoprotein L) and toxA (encoding exotoxin A i.e. ETA), are predominantly involved in, P. aeruginosa-related infections. Materials & Methods: A total of 120 specimens of the bacteria Pseudomonas aeruginosa were collected from Veterinary microbiology and various hospital laboratories. The isolates were initially identified by culturing on MacConkey agar and Eosin Methylene blue (EMB) agar and were further characterized by morphological and biochemical tests. An antibiotic sensitivity test was carried out on 13 antibiotics using the disc diffusion method. Genotypic detection of oprL and toxA genes was performed using a specific PCR test. Results: The results revealed that the toxA gene was detected by 84.62% in isolates belonging to human samples and 75% in the isolates of animal samples, whereas the oprL gene was detected by 80.77% and only 16.67 % in the isolates were derived from human and animal samples, respectively. Conclusion: The PCR analysis can help in the fast and specific detection of oprL and toxA genes in P. aeruginosa. Monitoring of these pathogenic genes could prevent the risk of transmission of multi-drug resistant P. aeruginosa, from animals to humans.  },  
Keywords = {Pseudomonas aeruginosa, antibiotics, multi-drug resistance, lipoprotein, exotoxin, PCR},
volume = {12},
Number = {4}, 
pages = {412-421}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i4.11443},
url = {http://jabs.fums.ac.ir/article-1-2864-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2864-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Liravi, Atefeh and Houshmand, seyed Massoud and Jafarnia, Mojtaba and Forouzanfar, Mohse},  
title = {Evaluation of AURKA rs2273535 and CDKN1B rs34330 Polymorphisms Association with the Risk of Breast Cancer}, 
abstract ={Background & Objective: Breast cancer consists of a heterogeneous group of tumours with different prognosis and is the most common cause of cancer-related mortality among women. In this study, our goal was to evaluate the association of AURKA rs2273535 and CDKN1B rs34330 polymorphisms with risk of female breast cancer in southwest part of Iran. Materials & Methods: This case-control study was done on 50 women with breast cancer and 50 healthy women without any symptoms or family history of breast cancer as the case and control group, respectively. Restriction fragment length polymorphism (RFLP- PCR) technique was designed to determine the AURKA rs2273535 and CDKN1B rs34330 gene polymorphisms. Afterwards, statistical analysis was done by means of SPSS (version 17). Results: In current research, our finding showed that rs2273535 T allele increased the susceptibility of breast cancer (OR:2.58, 95%CI:1.5-31.09, P=0.006). Moreover, T allele in dominant phase could raise the risk of the breast cancer (OR:3.01, 65%CI:1.31-6.92, P=0.009). However, the other polymorphism, CDKN1B rs34330, revealed no associations with increased risk of breast cancer. Conclusion: These findings suggest that AURKA rs2273535 may influence individual’s susceptibility to breast cancer. But we found no associations regarding CDKN1B rs34330 polymorphism and this type of cancer.  },  
Keywords = {Breast cancer, AURKA, CDKN1B, rs2273535, rs34330, RFLP},
volume = {12},
Number = {4}, 
pages = {422-430}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i4.11444},
url = {http://jabs.fums.ac.ir/article-1-2869-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2869-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Rahimi, Heidar and Rashidian, Ehsean and Shams, Nemat and Jaydari, Ami},  
title = {Multiple Locus Variable-Number Tandem Repeat Analysis Genotyping of Human Brucella Melitensis Isolates from West of Iran}, 
abstract ={Background & Objective: Brucellosis is an endemic disease with a high prevalence in Iran whose highest frequency is in the western region of the country. Genetic diversity investigation is an important method to determine the epidemiological relationship of Brucella isolates in different geographical areas. Therefore, the present study aimed to investigate the genetic diversity of human Brucella melitensis (B. melitensis) strains using the Multiple Locus Variable-Number Tandem Repeat Analysis (MLVA) Typing method in the west of the country. Materials & Methods: In this study, 20 strains of isolated B. melitensis were collected from the human serum samples of suspected Brucellosis in the west of the country and were analyzed by MLVA-16 method. Results: The results showed that 3 genotype numbers 42, 43 and 47 were identified using MLVA-8 method and using MLVA-11 method genotypes 125, 138 and 111 were recognized. Also, 16 different genotypes were detected from the analysis of the isolates by MLVA-16 method which shows a high degree of polymorphism among the isolates due to the high genetic diversity of the isolates in Panel 2B loci. Conclusion: The results showed the high genetic diversity of B. melitensis isolates in the west of the country and their genetic relationship with the known strains in the neighboring countries of the Eastern Mediterranean area, as well as the importance of the MLVA method in identifying the source of infection.},  
Keywords = {Brucellosis, Brucella melitensis, Genotype, MLVA, Human, Iran},
volume = {12},
Number = {4}, 
pages = {431-438}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i4.11445},
url = {http://jabs.fums.ac.ir/article-1-2909-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2909-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}

@article{ 
author = {Hosseini, Parvin Sadat and Rafieirad, Maryam and Esmaeili, Somayeh},  
title = {The Effect of Intraperitoneal Administration of Oleuropein on Pentylenetetrazole Induced Epilepsy in Male Rat}, 
abstract ={Background & Objective: Epilepsy is one of the most common neurological disorders in humans, which appears as sudden, episodic, repetitive, and unpredictable seizures, and these patients suffer from memory impairment. Medicinal herbs have long been used to treat epilepsy. In this study, the impact of effective oleuropein on the time of arising of attacks and the rate of attack's incidence of tonic, clonic, tonic-clonic, and total seizure longevity in male rats was investigated.  Materials & Methods: Forty rats were randomly divided into four groups of 10. The negative control group (the recipient of normal saline), the positive control group (the recipient of diazepam 1 mg/kg), and the two treatment groups (the recipient of doses of 10 and 20 mg/kg of oleuropein). After 30 min of oleuropein administration, saline, or diazepam, pentylenetetrazole was injected intraperitoneally into rats' bodies at a dose of 85 mg/kg, and 30 min after injection, seizure parameters were evaluated. Data were analyzed by one-way ANOVA and Tukey's statistical tests.  Results: Injection of the oleuropein significantly increased the threshold of seizures in the form of initial delay at the arising of seizure in the positive control group and treatment groups (P< 0.001). Furthermore, it reduces the time of seizures in different phases (P< 0.001). In addition, a dose of 10 mg/kg oleuropein has the highest effect on total seizure longevity.  Conclusion: This study showed that oleuropein has an appropriate anti-seizure effect and future studies appear to be necessary for further understanding of the mechanism.},  
Keywords = {Oleuropein, seizure, PTZ},
volume = {12},
Number = {4}, 
pages = {439-445}, 
publisher = {Fasa University of Medical Sciences},

doi = {10.18502/jabs.v12i4.11446},
url = {http://jabs.fums.ac.ir/article-1-2881-en.html},  
eprint = {http://jabs.fums.ac.ir/article-1-2881-en.pdf},  
journal = {Journal of Advanced Biomedical Sciences},  
issn = {}, 
eissn = {2783-1523}, 
year = {2022}  
}