@article{ author = {Maghbol, Maryam and ashraf, Mohammad Javad and Azarpira, Negar}, title = {Parotid Lipoma: Case Report}, abstract ={Background & Objective: Lipoma (a benign tumor composed of adipose tissue) is the most common benign mesenchymal tumors in soft tissue but it is very rare in the parotid gland. Case: We reported a 54 years old man with parotid gland lipoma. Parotid gland lipoma usually originates from the superficial lobe. Superficial parotidectomy has been considered as the choice method of the treatment. Result: lipoma, as a prevalent tumor of the soft tissue, is rarely seen in salivary glands.}, Keywords = { Parotid gland, lipoma }, volume = {4}, Number = {4}, pages = {371-374}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-497-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-497-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2014} } @article{ author = {Bijani, Mostafa and Valizadeh, Abbas and Sayari, Amenh and Samizadeh, Babak}, title = {Surveying the Reasons for Refusing Coronary Angiography in Patients Referring to Cardiac Ward of Valiasr Hospital in Fasa}, abstract ={ Background ; Objective: Coronary angiography has become the largest interventional diagnostic test in recent years. As it has had large effects on anxiety and satisfaction of patients in treatment and care the objective of the present study is to survey the reasons for which the patients, referring to cardiac ward of Valiasr hospital in Fasa, refuse to undergo coronary angiography.  Materials ; Methods: In this cross-sectional study, 60 individuals candidate for coronary angiography were selected. The samples were selected via census. The tool for collecting the data was a researcher-made questionnaire. The data were analyzed by SPSS-15 software, descriptive statistics, Mann-Whitney test, and Kruskal- Wallis test.  Results: The mean age of the samples was 58/9±12/8 years. Financial problems, fear and anxiety, lack of awareness, and lack of confidence in the physician’s diagnosis were the most important factors. The effect of fear has been reported more in women compared to men for refusing coronary angiography (p=.037).  Conclusion: According to the above results, financial problems, fear and anxiety, lack of awareness, and lack of confidence in the physician’s diagnosis are the factors effective in accepting coronary angiography. Therefore, more contrivances are needed to eliminate these effective factors in order to prepare cardiac patients, who are candidate for coronary angiography, a tranquility to accept and to perform angiography.   }, Keywords = {Effective factors, Coronary angiography, Cardiac patients}, volume = {4}, Number = {4}, pages = {375-381}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-370-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-370-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2014} } @article{ author = {Mamnoon, Babak and NaserpourFarivar, Taghi and KarimiArzenani, Mohse}, title = {Application of Rapid and Sensitive Real Time PCR Technique in Detection of DNA Impurities in Recombinant Interferon}, abstract ={ Background & Objective: Interferon belongs to a family of cytokines, which has the most important role in the innate immune response to virus infections. While producing recombinant interferon in biological host, some pieces of host nucleic acids remain in product. Because of limitations in previous techniques for detection of these impurities, the objective of this study is to use rapid and sensitive Real time PCR method for detecting the impurities.   Materials & Methods: First, with DNA extraction from bacterial host cell and preparation of its serial dilutions, SYBR Green-based Real time PCR reaction was held and standard curve was plotted. After DNA extraction from interferon and performing PCR, total DNA amount was determined using standard curve.   Results: Studies performed on some interferon samples, revealed that the amount of DNA impurities was about 0.02 pg. per product dose. In addition, the designed primers in the above reaction had no interaction with each other and other interfering agents.  Conclusion: For the first time in Iran, this study was set up and it revealed that Real time PCR can be used as a functional and accurate technique in manufacture centers for detection of residual host cell DNA in interferon and other recombinant pharmaceutical products.  }, Keywords = {DNA impurities, Recombinant pharmaceutical products, Interferon, Real time PCR, SYBR Green I}, volume = {4}, Number = {4}, pages = {382-391}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-237-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-237-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2014} } @article{ author = {Kashfi, Mansour and Yazdankhah, Maryam and Khanijeihooni, Ali and karimi, mojtab}, title = {Evaluating the frequency of Self-Immolation and its Relationship with Social and Demographic Status of the Patients Referring to Ghotboddin E Shirazi during the Years 2006 and 2011}, abstract ={ Background & Objective: Suicide is the act of injuring oneself in order to destruct one’s life. One of the worst ways to attempt suicide, especially in eastern countries, is self- burning. This study aimed to show the frequency of self- burning and its relationship with social and demographic status.  Materials & Methods: In this cross-sectional study, all medical records of patients who admitted to Ghotbeddin E Shirazi hospital for burns during the years 2006 and 2011 were collected. The data were analyzed by SPSS-16 software, descriptive statistical methods and chi-square tests.  Results: The results showed that from 2071 cases of burn patients admitted to Ghotbeddin E Shirazi Hospital, 439 cases (21.1%) have been caused by self-immolation. The proportion of self-immolation among women was 70.7%, in married was 58.4%, and in rural population was 56.6%. It was most prevalent in the age group of 25-44 years old (47.4%). In most cases, self-immolation was occurred with flammable liquids especially oil and gasoline (71.8% and 17.3%, respectively) and at home (90/8%). 67.8% of cases lead to death. (Case-fatality rate was 67.8%). The most cause of self-immolation attempting was family disputes.  Conclusion: High self-immolation rate in females and the age group of adolescents and youth is significant which should be considered as an important issue in terms of health.   }, Keywords = {Suicide, Burning, Social status}, volume = {4}, Number = {4}, pages = {392-401}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-198-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-198-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2014} } @article{ author = {Mahaldashtian, Maryam and Naghdi, Majid and Ghorbanian, Mohammad Taghi and Koruji, Morteza and Makoolati, Zohre and Naghizadeh, Mohammad Mehdi and Kouhpayeh, Seyed Amin and Abdollahi, Abbas and Astaneh, Mohammad Ebrahim}, title = {The effect of aqueous extract of Phoenix Dactylifera Pollen on In vitro viability and proliferation rate of neonatal mouse spermatogonial stem cells}, abstract ={Introduction: There is a fast growing tendency in the consumption of herbal remedies in the developing countries. One of the traditional medicines used for male infertility is Date palm (Phoenix dactylifera) pollen (DPP). The goal of the present study was to investigate the effect of aqueous extract of DPP on In vitro viability and proliferation rate of neonate mouse spermatogonial stem cells (SSCs). Methods: cell suspension includes sertoli cells and SSCs were isolated from neonatal 6 day-old mice testes by 2 steps enzymatic digestion. The cell suspension was cultured in DMEM and FCS 4% in the absence or presence of 0.06, 0.25 and 0.62 mg/ml of aqueous extract of DPP for 2 weeks. In order to evaluate the rate of SSCs expansion at the end of culture, the mean number of whole cells and living cells were considered as proliferation and survival rates respectively. Data analysis was done with ANOVA test. The significancy of the data was analyzed using ANOVA and Tukey post test. Results: The results showed that there were no significant differences between the mean percent of viability and proliferation rate between control and 0.06, 0.25 and 0.62 mg/ml of DPP-treated groups (P> 0.05). Conclusion: Our study showed that treatment of neonatal mouse testicular cell suspension with DPP had no toxic effects on viability percent and proliferation rate of these cells. Thus, we can use DPP for evaluate the in vitro pattern of SSCs colonization in the future studies.}, Keywords = {Spermatogonial Stem Cell, Date Palm Pollen, viability, proliferation}, volume = {4}, Number = {4}, pages = {402-408}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-461-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-461-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2014} } @article{ author = {Ghaffari, Hamidreza and Gholami, Soghra and Naghdi, Majid and AlipoorTabrizi, Malihe}, title = {Histologic and Histomorphometric Study on the Effect of Hydro-Alcoholic Aloe Vera Extract on Tissue Formation of Sciatic Nerve in Diabetic Male Rats}, abstract ={ Background ; Objective: Diabetes mellitus affects the metabolism of carbohydrates, lipids, and proteins, which leads to the dysfunction of the central and peripheral nervous system. Reports suggest that Aloe Vera has anti-diabetic effects. Therefore, this study was carried out to determine the effect of hydro alcoholic extract of Aloe Vera on Sciatic nerve of the diabetic rats.Materials ; Methods: 45 male Sprague-Dawley rats weighing 200-250 gram were randomly divided into three groups of 15 rats as control group, the experimental group1 (DM), and the experimental group 2(DM+ Aloe Vera). Diabetes in the experimental groups of one and two was induced by injection of 50mg/kg Streptozotcin. The control group and the experimental group two received 50mg/kg Aloe Vera by gavage for 12 and 16 weeks. Then, the animals were anesthetized and sciatic nerves were dissected and fixed. The tissue changes of the sciatic nerves were surveyed by histologic and histomorphometric studies. The data have been analyzed by using the SPSS software  Results: In diabetic rats, a significant decrease was seen in the mean diameter of the myelinated nerve fibers and myelin sheath thickness of the Sciatic nerve. Long-term treatment with Aloe Vera significantly prevented all these abnormalities in treated diabetic rats (P <0/05). Conclusion: Our findings showed that the hydro alcoholic extract of Aloe Vera as a potential therapeutic agent that can help prevent histomorphometric and histologic changes induced by diabetic peripheral neuropathy.  }, Keywords = {Aloe Vera, Diabetic neuropathy, Sciatic nerve, Rat}, volume = {4}, Number = {4}, pages = {409-417}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-208-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-208-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2014} } @article{ author = {Moradi, Azam and Ebrahimipour, Gholamhossein and Karkhane, Maryam and Marzban, Abdolrazagh}, title = {Surveying the Antioxidant and the Antimicrobial Effects of Aqueous and Ethanolic Extract of Rumex Alveollatus L. on In-vitro Indicator Microorganisms}, abstract ={ Background ; Objective: In recent years, more attention has been devoted to herbal medicines. Up to now, many compounds with therapeutic effects has been extracted from the herbs. The aim of this study is to evaluate the antioxidant and the antimicrobial effect of Rumex Alveollatus L. and to partially identify the effective compounds in this plant. Materials & Methods: Extraction was performed by using maceration method for dried flower sample. Then, the antimicrobial effect of aqueous and ethanolic extracts on eight bacterial sp. and two fungi were tested using disc diffusion method. The antioxidant effect was also determined through ferric reducing potency and phosphomolybdenum followed by total phenol determination. Finally, partial detection of bioactive compounds was conducted using chemical and calorimetric methods.  Results: The results showed that ethanolic extract had the most antimicrobial effect while aqueous extract weakly affected bacterial and fungal strains. Antioxidant experiments also revealed that ethanol extract had more antioxidant effects than aqueous extract. The most content of total phenolic compounds was found in ethanol extract. The results of the plant chemical determination showed the presence of flavonoids, alkaloids, anthraquinones, tannins, glycosides, and reducing sugars. Conclusion: Considering that few reports about the therapeutic effect of Rumex alveollatus L. has been published, this study could be considered as a valuable report about the important role of this plant on preventing infections and neutralizing oxidant agents.   }, Keywords = {Rumex alveollatus L, Antioxidant effect, Antimicrobial effect, Aqueous extract, Ethanol extract}, volume = {4}, Number = {4}, pages = {418-426}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-579-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-579-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2014} } @article{ author = {Majidi, Nasrin and Movahedin, Mansoureh and Mazaheri, Zohre}, title = {Evaluating the Development and the Expression of Bax, Bcl-2, and ErbB4 Genes following Vitrification of Eight Cell and Blastocyst Embryos}, abstract ={ Background & Objective: Vitrification is an effective mean for preserving embryos. During this process, expression of implanting and apoptotic genes changes and can affect the quality of embryos. In this study, the expressions of Bax and Bcl-2 as apoptotic genes and ErbB4 as implanting gene were investigated in vitrified and warmed embryos.  Materials & Methods: In this study, six-eight week old female (NMRI) mice were used. Their ovaries were hyper stimulated with 7.5 IU PMSG and 7.5 IU HCG. Females were mated with males from the same strain and inspected for the presence of vaginal plugs on the following morning. Females with the presence of vaginal plugs were considered to be pregnant and were killed 62 h post HCG injection. 8-cells embryos were flushed from oviduct and were divided to three experimental groups including fresh, vitrified eight-cell embryos and vitrified blastocyst embryos. Finally, RNA was extracted and expression of Bax, Bcl-2, and ErbB4 genes was evaluated by Real time PCR. Data were analyzed by Chi-square and ANOVA tests.  Results: The results of this study showed that there was not any significant difference in blastocyst formation and degeneration between three experimental groups. In addition, there was not any significant difference in relative gene expression of Bax, Bcl-2, andErbB4 between all the three groups.  Conclusion: According to the result of this study, vitrification-thawed process does not have any unpleasant effect on the development of eight cell and blastocyst embryos. It does not have negative effect on Bax, Bcl2, and ErbB4 genes expression.    }, Keywords = {Vitrification, Blastocyst, Implantation}, volume = {4}, Number = {4}, pages = {427-435}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-540-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-540-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2014} } @article{ author = {Karimi, Mohammad Hossein and Japoni, Aziz and Rasouli, Manouchehr and Ebrahimnezhad, Salimeh}, title = {The Immunomodulatory Effect of Recombinant Exotoxin A of Pseudomonas Aeruginosa on Dendritic Cells Extracted from Mice Spleen}, abstract ={ Background & Objective: Dendritic cell (DC) is as a key cell in activation of immune response against microbes and disease. Therefore, the effect of recombinant exotoxin A of Pseudomonas aeruginosa on the maturity and the activation of DCs was evaluated in this study. Materials & Methods: Recombinant exotoxin A was produced from Pseudomonas aeruginosa DNA. MTT assay was used to evaluate the cytotoxicity of this protein on DCs. The expression of co-stimulatory molecules CD40, CD86, and MHCΠ was evaluated by flow cytometry. Moreover, the effect of this antigen (Ag) on T-cell proliferation was evaluated using Mixed Lymphocyte Reaction (MLR) assay and the secretion of IL-4 and IFN- γ. Secretion of IL-12 by DCs was measured with Enzyme-Linked Immunosorbent Assay (ELISA) method. The data were collected and analyzed with one way ANOVA test. Results: Recombinant exotoxin A had no effect on DCs viability. In addition, expression of CD40, CD86, and MHCΠ did not change significantly compared to the negative control cells. Moreover, T-cells proliferation was decreased significantly at the concentration of 0.1µg/ml of this Ag. The secretion of IL-12 was increased by DCs, in contrast the secretion of IL-4 and IFN-γ in MLR supernatant did not decrease significantly. Conclusion: Exotoxin A decreases the proliferation of T-cells and also leads to a change in the pattern of cytokine secretion of immune cells.  }, Keywords = {Exotoxin A, Dendritic cell, Immunomodulatory effects}, volume = {4}, Number = {4}, pages = {436-445}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-508-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-508-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2014} } @article{ author = {jafarzadeh, saeedeh and mohamadkhankermanshahi, sima and Hajizadeh, Ebrahim}, title = {The effect of Health Promotion Program with Collaborative Approach on Body Weight of Overweight Elementary School Girls}, abstract ={ Background & Objective: Increase trend of overweight in recent years has become a concerning matter, which is a health alarm for children. Therefore, the present study investigated the impact of health promotion programs with collaborative approach on the weight of overweight school age girls. Materials & Methods: This quasi-experimental study was conducted in 2013, in which 80 overweight students in Firozabad were selected by non-randomized method and were divided into case and control groups. The data collecting tools were demographic information check list, electronic scale, and stadiometer. In the case group, health promotion programs with three stages of assessment, supportive planning, and evaluation was administered for three months. Children’s weights in both groups were evaluated before and three months after intervention. The results were analyzed by SPSS software version 16. Results: Case and control groups were equal from the view point of average weight before the intervention (P>./05).While after intervention, "independent T test" showed a significant different between two groups in average weight after intervention (P<0/05). The paired T-test showed that the mean scores of weight for the case group decreased from 48/95 to 47/39 after intervention (P<0/05). However, the results showed that the mean scores of weight in the control group increased from 50/08 to 52/76 after study (P<0/05). Conclusion: Health promotion programs with collaboration of children, parents, and teachers could reduce the weight of overweight children. Therefore, it is proposed for using this program in order to prevent overweight and obesity in school age children.  }, Keywords = {Health promotion programs with collaborative approach, Weight, Overweight school girls}, volume = {4}, Number = {4}, pages = {446-455}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-492-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-492-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2014} } @article{ author = {Ahadnejad, Mohsen and Ghaderi, Hosein and Hadian, Mohammad and Haghighatfard, Payam and Darvishi, Banafsheh and Haghighatfard, Elham and Zegordi, Bitasadat and Bordbar, Arash}, title = {Location Allocation of Health Care Centers Using Geographical Information System: region 11 of Tehran}, abstract ={ Background & Objective: Location allocation of healthcare centers facilitates the accessibility of health services and the lack of proper distribution of these centers leads to increasing problems of citizens' access to these centers. The main objective of this study was to evaluate the distribution of healthcare centers in the region of the study and to determine deprived areas from this services. Materials & Methods: This research is a case study that has been done on region 11 of Tehran. The data were collected form maps and they have been analyzed by Analytical Hierarchy Process (AHP) in GIS environment. Results: There are 10 hospitals in the study area that include seven percentage of all hospitals in Tehran. In the evaluation of the information layers, the layers of the residential and the industrial centers were respectively weighted %19(maximum weight) and %1(minimum weight). After the compatibility analysis, the rate of incompatibility of different lands with treatment land was four percentage. This study showed overlapping services of existing hospitals by determining service radius of 1500 m. In addition, with combining information layers on the final map, suitable areas for optimal placement of health centers were identified. Conclusion: The results of this study showed that current locations of medical centers in region11 of Tehran do not match with scientific standards. }, Keywords = {Location allocation, Healthcare centers, AHP, GIS}, volume = {4}, Number = {4}, pages = {463-474}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-234-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-234-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2014} } @article{ author = {Lohrasb, Mohammad Hossein and Ghodrat, Mahshid and Meshkibaf, Mohammad Hass}, title = {Therapeutic Effects of Topical Minoxidil or Rosemary and the Combination of Both on the treatment of Alopecia areata}, abstract ={Background & Objectives: Considering the prevalence of Alopecia areata, , failure of treatment, and the unknown pathogenesis of this illness, a comparative study was performed by using topical Minoxidil 2% and topical rosemary solution alone and in combination to treatment this disease. Materials & Methods: This study is a clinical trial performed on 200 patients with Alopecia areata referring to Hamzeh clinic of Fasa during the years 2012 and 2013. They were divided into four groups by random permutation, each group contained 50 patients. Group one received the combination of topical Minoxidil 2% and topical rosemary, group two received only topical Minoxidil 2% solution, group three received only topical rosemary solution and the fourth group, the case-control group, did not receive any medication and were just advised to rub the site of the disease for the same period of time. The patients were under observation for one year. Results: The Results of this investigation showed that the best remissions after treatments were as follow (respectively): combination of topical Minoxidil 2% and topical rosemary (27 patient=54 %), Minoxidil 2% solution (23 patients =46%), rosemary solution (21 patients =42%), and case- control group (9 patients =18%). These results showed that despite better response to the combination of rosemary and Minoxidil solutions in comparison to the two other treated groups, the changes were minimal and statistically insignificant (P-value =0.0411). Conclusion: Using the combination of both rosemary and Minoxidil is more effective than the individual one on treatment of Alopecia areata.}, Keywords = {Alopecia areata, Rosemary, Minoxidil }, volume = {4}, Number = {4}, pages = {475-484}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-568-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-568-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2014} } @article{ author = {molazade, alireza and shahi, abbas and najafipour, sohrab and mobasheri, farzane and Norouzi, Fateme and AbdollahiKheirabadi, sara and AshrafMansoori, seyed jalal-ad-din and gholami, mohammadSaee}, title = {Antibiotic Resistance Pattern of Bacteria Causing Urinary Tract Infections in Children of Fasa During the years 2012 and 2014}, abstract ={Background & Objectives: Urinary Tract Infection (UTI) is one of the most prevalent bacterial infection in children. Awareness of the antibiotic resistance of common bacteria causing UTI in each area has a large impact on infection improvement. The objective of this study is to evaluate the antibiotic resistance of bacteria causing UTI in two to 15 years old children referring to Fasa Vali-e-Asr hospital during 2012 and 2014. Materials & Methods: This cross sectional - descriptive study was done on 283 children with UTI that had positive urine culture. After identification of strains, the antibiotic sensitivity definition test, using antibiogram disk diffusion method, was done. Results: In this study, the most frequent isolates were Escherichia coli 64.3%, Klebsiella 14.5%, and staphylococci 6.4%. The highest sensitivity rate was to Ciprofloxacin and Nitrofurantoin and the highest resistance was to Co-trimoxazole and Cefalotin. Conclusion: Regarding the results, it is recommended to use Ciprofloxacin and Nitrofurantoin for outpatient treatment of UTI. Selecting proper antibiotics for UTI treatment should be on the basis of the local prevalence of pathogenic bacteria and antibiotic resistance pattern.}, Keywords = {Urinary Tract Infection (UTI), Children, Antibiotic resistance}, volume = {4}, Number = {4}, pages = {493-499}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-597-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-597-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2014} }