@article{ author = {Fanaei, Hamed and Azizi, Yaser and Khayat, Samir}, title = {A Review: Role of oxidative stress in male infertility}, abstract ={Recent studies have shown that reactive oxygen species (ROS) have a very important role in the intracellular signaling process in physiological conditions. On the other hand, during the recent decade it has been indicated that ROS play a role in various types of male infertility and it is due to the overproduction of ROS or decrease in the antioxidant defense system in the reproductive system and sperm. In pathological conditions, ROS via interferences in the spermatogenesis process, sperm function, and sperm structure (motility, viability, acrosome reaction, sperm-oocyte fusion, and damage to DNA and cell membrane) as well as reduction in fertilization and implantation can lead to infertility. Knowledge of how ROS affect the physiological process of the reproductive system is crucial in the treatment of infertility. Thus, in this review article we will discuss experimental and clinical findings related to the effects of ROS on male fertility.}, Keywords = {reactive oxygen species, antioxidant, sperm, male infertility}, volume = {3}, Number = {2}, pages = {93-103}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-337-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-337-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2013} } @article{ author = {Chamanrokh, Parastoo and Shahhosseiny, Mohammad hassan and MazaheriAssadi, Mahnaz and Nejadsattari, Taher and Esmaili, Davoo}, title = {Comparison PCR Method and Rapid Urease Test to Detect Helicobacter Pylori in the Gastric Biopsy Tissue Samples}, abstract ={Background & Objective: Helicobacter pylori has been recognized as a major risk factor in gastric and duodenum ulcers and gastric cancer. Some laboratory tests, such as culture, are not entirely satisfying. The aim of this study is to compare RUT with PCR in identifying H. pylori in the gastric biopsy tissue samples.Materials & Methods: First, the standard H.pylori sample (N:oC30) was provided. primers or the glmM (ureC) gene were used In PCR method The PCR test was optimized by sensitivity and specificity methods. Then 100 clinical samples composed of stomach tissue biopsy were prepared. The rapid urease test was conducted on all obtained samples to identify H.pylori. DNA was extracted from samples using DNG plus technique. Then, samples were studied using PCR method.  Results: The 294 bp product was amplified in the optimized PCR test. The PCR test sensitivity was obtained at 10 CFU. Any unwanted products were not seen in the specificity test with the exception of H. pylori DNA samples. Of 100 stomach biopsy samples, 64% were reported as positive by RUT and 76% by the PCR test.Conclusion: Given that the PCR test has higher sensitivity and specificity to detect H.pylori comparing rapid ureaas test, therefore this method could be used to detect H.pylori.  }, Keywords = {Helicobacter pylori, glmM, PCR method, rapid Ureas test}, volume = {3}, Number = {2}, pages = {104-110}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-163-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-163-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2013} } @article{ author = {Zamiri, Hamid Khosro and Noroozi, Mehrdad and Moradi, Siavash and Shabani, Mohammad and Sharifi, Ali and Haghbin, Mohammad Ali}, title = {Evaluation of intra ocular pressure and hemodynamic change following intubation with Maccoy, Macintosh and Video laryngoscope}, abstract ={Background & Objective: The induction of anesthesia, laryngoscopy and endotracheal intubation can be associated with adverse hemodynamic response and increased intraocular pressure. The aim of this study was to evaluate intraocular pressure and hemodynamic changes after laryngoscopy and endotracheal intubation with three methods of laryngoscopy (Macintosh, Maccoy and Video laryngoscope).Materials & Methods: One hundred and eighty patients with American Society of Anesthesiology (ASA) classification of I and II, aged 20-70 year, were enrolled in a randomized clinical trial (RCT). Anesthesia was induced by administration of Propofol 2 mg/kg, Fentanyl 1µg/kg and Cisatracurium 0.1mg/kg. the Hemodynamic information of the patients and intraocular pressures were documented and assessed in three stages (after induction of anesthesia and endotracheal intubation, and 5 minutes after endotracheal intubation using Maccoy, Macintosh and Video laryngoscope).Results: Hemodynamic parameters' of patients increased in these three groups compared with those of pre anesthesia measures, but this increase was not significant. Evaluation of intraocular pressure (IOP) in Video laryngoscope group showed that there is a significant drop in intraocular pressure (IOP) compared with other groups. There weren’t any significant differences of IOP after intubation in Maccoy compared to the Macintosh group. Nevertheless there was not any significant difference in IOP, before and five minutes after intubation in these groups.Conclusion: According to a low degree of augment of IOP in Video laryngoscope group and no changes in the Hemodynamic parameters, it seems that the use of Video laryngoscope in eye surgeries might be more suitable for endotracheal intubation.}, Keywords = {Hemodynamic responses, Intraocular pressure, Laryngoscopy, Video laryngoscope}, volume = {3}, Number = {2}, pages = {111-116}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-107-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-107-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2013} } @article{ author = {javanmardi, kazem and soltanihekmat, ava and shekoohi, masoomeh and hasanein, parisa and bakhshi, mehdi and ghodsi, roghayeh and rezaeian, leila and Bordbar, Akhtar}, title = {The Role of Parabrachial GABAA Receptors in Pain Modulation in Rats}, abstract ={Background & Objective: The parabrachial nucleus is a critical link in the transmission of short latency nociceptive information to midbrain neurons. GABA(A) receptors have bidirectional roles in controlling nociception and are abundant in the parabrachial region . We examined the effects of bilateral intra parabrachial microinjection of different doses of the GABA(A) receptor agonist, muscimol, and the GABA(A) receptor antagonist, bicuculline, on pain modulation using a tail-flick test . Materials & Methods: Rats were anaesthetized with sodium pentobarbital (55 mg/kg) and then special cannulas were inserted stereotaxically into the parabrachial nucleus. After 1 week of recovery, the effects of microinjection of muscimol, (62.5, 125,250 ng/side) or bicuculline, (50,100,200 ng/side) into the parabrachial on tail flick latencies were assessed. Tail-flick latencies were measured for 60 minutes every 5 min after drug microinjection. Results: Microinjection of muscimol (62.5, 125 ng/side) and bicuculline (50,100,200 ng/side) into the parabrachial did not have any statistically significant effect on tail-flick latency. Administration of, muscimol, (250 ng/side) produced thermal hyperalgesia (P<0.05). Conclusion: The results of the present study showed that in this model of pain gaba a receptors in the paracrachial region are not Endogenously activated but these receptors in this region have a potential to affect pain modulation.}, Keywords = {Parabrachial, , GABAA Receptors , Pain}, volume = {3}, Number = {2}, pages = {117-123}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-282-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-282-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2013} } @article{ author = {Sohrabi, Nooshin and Azimi, Shohreh}, title = {Cloning and Expression of Leptospira LipL32 Antigen as a Candidate for Rapid Diagnosis}, abstract ={Background and Objective: Leptospirosis as an important emerging infectious zoonotic disease caused by spirochetes of the genus Leptospira. Given the low sensitivity and long duration of its culture, the diagnosis of leptospirosis is mainly based on serological methods. The microscopic agglutination test (MAT) is considered as the reference method. Because of the complexity of the MAT, there is an urgent need for the development of new reliable and rapid screening tests for leptospirosis. Major leptospiral outer membrane proteins (OMPs), present only in pathologic strains, could be regarded as a good candidate for diagnostic studies. Here we report the cloning and expression of LipL32, as a prominent immunogenic protein, in a prokaryotic system. Materials and Methods: After the amplification of LipL32 gene, it was cloned into the pQE30 vector. The insertion of LipL32 gene into the vector was screened and confirmed with restriction analysis and sequencing. The recombinant plasmid was transformed into E. coli M15 strain, and the expressed protein was identified by SDS-PAGE and western blotting. This recombinant protein with 6× His-tagged sequence was purified using Ni-NTA affinity column chromatography. Results: The results revealed that the selected gene was successfully cloned in pQE30 vector and recombinant protein (rLipL32) of approximately ~32 kDa was produced, purified and confirmed by western blotting. Conclusion: This recombinant protein could be potentially used for the development of serodiagnosis tests for the diagnosis of leptospirosis in humans and animals.}, Keywords = {Leptospirosis, Recombinant Protein, Cloning, LipL32, Protein expression}, volume = {3}, Number = {2}, pages = {124-129}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-29-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-29-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2013} } @article{ author = {hedayatirad, fatemeh and sharifan, anooshe and KhodayianChegini, faramarz and hossini, ebrahim}, title = {Antimicrobial activity of Pullulan film incorporated with Artemisia sieberi essential oil}, abstract ={Background & Objective: A large number of non-recyclable packaging materials, which are harmful for the health of consumers, are produced all over the world. The Suggested solution is using films and coatings which are biodegradable in nature. Pullulan is a biopolymer that is produced by Aureobasidium pullulans and used in order to produce edible films. These edible films could contain antioxidant and antimicrobial agents. The primary aim of this study is to produce edible blended films that form pullulan whit Artemisia sieberi essential oil and the secondary goal is to investigate the antimicrobial attributes of the obtained film. Materials and Methods: The Essential oil of Artemisia sieberi was added to the solution of pullulan films in seven concentrations. Then, the antimicrobial attributes were determined with the dick diffusion method. Finally, minimal inhibitory concentration was calculated for each microbe. The SAS program (Version 9.1) was used for the completely randomized design of data analyses. Results: It is possible to produce pullulan film containing Artemisia sieberi essential oil. Escherichia coli and Staphylococcus aureus had maximum and minimum resistance towards the antimicrobial attributes of Artemisia sieberi essential oil, respectively. MIC of Artemisia sieberi essential oil against Staphylococcus aureus, Lactobacillus plantarum and Escherichia coli were respectively obtained at 15%, 10% and 15%. Conclusion: Due to the possibility of the production of biodegradable films with the addition of natural antimicrobial agents such as essential oils it can be concluded that it is appropriate to use these materials in order to decrease microbial load and increase the shelf life of foods.}, Keywords = {Film, Pullulan, Essential oil, Artemisia sieberi }, volume = {3}, Number = {2}, pages = {130-135}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-165-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-165-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2013} } @article{ author = {minaei, Mohammad Ebrahim and Saadati, Mojtab}, title = {Double Sandwich ELISA Modified Method for the Detection of Clostridium Botulinum Type E}, abstract ={Background & Objective: A very small amount of botulinum toxin can cause death and on the other hand, there is no cure for its poison other than antitoxin. Therefore, a diagnostic method that can detect very small amounts of botulinum toxin in a short time is very important. In this study, rapid and accurate detection of botulinum toxin type E has been performed with the double sandwich ELISA method. Materials & Methods: based on the double sandwich ELISA method two different antibodies that bind to epitopes without the interference of antigens is required. For this purpose, the rabbit antibody was poured at the bottom (Capture antibody) and mouse antibody at the top of the toxin (Detection antibody) of each well and the toxin was titrated from the first wells. Then, the modified sandwich ELISA system was set up and optimized for the detection of botulinum toxin type E.Results: The modified double sandwich ELISA method is able to detect Clostridium botulinum type E toxin to a value of 1 ng in less than 6 hours. Conclusion: The modified double sandwich ELISA method is very precise and fast in detecting Clostridium botulinum type E. This method is used for determining specific antigen in the unknown sample.}, Keywords = { Clostridium botulinum, botulinum toxin type E, antibodies, double sandwich ELISA}, volume = {3}, Number = {2}, pages = {136-142}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-102-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-102-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2013} } @article{ author = {Hosseini, Seyed Ebrahim and Heidari, Mozhde and Aqababa, Heydar}, title = {The Effect of Salmon Fish’s Oil on Avoidance Learning in Mature Male Rats}, abstract ={Background & Objective: There is plenty of fatty acids of omega-3 in Salmon Fish that affect brain development and function, therefore, this study aimed to investigate the effect of Salmon fish’s oil on learning avoidance in mature male rats.  Materials ; Methods: In this empirical study, we used 40 mature male rats that were enrolled into control, sham and experimental groups. The control group was not treated. The sham group received only 1 ml saline daily, 3 experimental groups of different types received 0.25, 0.5 and 0.75 ml/kg body weight respectively, for 28 days. For the evaluation of avoidance learning behavior, the shuttle box was used. The data was evaluated using ANOVA.  Results: The results suggest that consumption of salmon oil significantly increases in minimum and maximum doses (P<0.01) and average doses (P<0.001) the process of avoidance learning. Conclusion: Salmon oil can lead to increased learning. Therefore, further investigation can be used to treat learning disorders and memory.  Normal 0 false false false EN-US X-NONE AR-SA /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal" mso-tstyle-rowband-size:0 mso-tstyle-colband-size:0 mso-style-noshow:yes mso-style-priority:99 mso-style-parent:"" mso-padding-alt:0cm 5.4pt 0cm 5.4pt mso-para-margin-top:0cm mso-para-margin-right:0cm mso-para-margin-bottom:8.0pt mso-para-margin-left:0cm line-height:107% mso-pagination:widow-orphan font-size:11.0pt font-family:"Calibri","sans-serif" mso-ascii-font-family:Calibri mso-ascii-theme-font:minor-latin mso-hansi-font-family:Calibri mso-hansi-theme-font:minor-latin}}, Keywords = {Salmon oil, Omega-3 fatty acids, Rat.}, volume = {3}, Number = {2}, pages = {169-173}, publisher = {Fasa University of Medical Sciences}, url = {http://jabs.fums.ac.ir/article-1-134-en.html}, eprint = {http://jabs.fums.ac.ir/article-1-134-en.pdf}, journal = {Journal of Advanced Biomedical Sciences}, issn = {}, eissn = {2783-1523}, year = {2013} }