en مطالعه‌ی مقاومت دارویی سویه‌های Acinetobacter جداشده از بیماران بستری در بخش ICU با روش MIC ميكروبیولوژی Microbiology پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Background & Objectives: </span></span></span></b><em><span style="font-size:12.0pt">Acinetobacter</span></em> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">spp. are non-fermentative, Gram-negative, opportunistic pathogens characterized by high levels of antibiotic resistance and are frequently associated with nosocomial infections in intensive care units (ICUs). This study aimed to investigate the antibiotic resistance profiles of <em>Acinetobacter</em> strains isolated from ICU patients using the BD Phoenix system, which determines antimicrobial susceptibility based on the <em>Minimum Inhibitory Concentration (MIC)</em> method.</span></span><span style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Materials & Methods: </span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">A descriptive, cross-sectional study was conducted from October 2018 to May 2019 involving 60 ICU patients at Mehr-e Hazrat Abbas Hospital in Mashhad, Iran. Clinical specimens&mdash;including blood, bronchoalveolar lavage, tracheal secretions, wound swabs, biopsies, pleural fluid, and sternum swabs&mdash;were collected under sterile conditions. <em>Acinetobacter</em> species were identified through standard culture techniques and confirmed via PCR targeting the <em>blaOXA-51-like</em> gene. Antimicrobial susceptibility testing against 21 antibiotics was performed using the BD Phoenix system in accordance with CLSI guidelines. Data were analyzed using SPSS software employing chi-square tests (p < 0.05).</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Results: </span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">All 60 isolates were confirmed as <em>Acinetobacter</em> spp. Colistin demonstrated the highest susceptibility rate (90%). The highest resistance rates were observed against aminoglycosides, carbapenems, and quinolones (each 55%), followed by cephalosporins, macrolides, and &beta;-lactamase inhibitors (54%), sulfonamides (43%), and monobactams (40%).<br> Multidrug-resistant <em>A. baumannii</em> represents a serious threat in ICUs owing to its environmental persistence, genetic adaptability, and plasmid-mediated resistance mechanisms. Although colistin remains one of the most effective therapeutic options, its use is limited by toxicity and the emergence of resistance.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Conclusion</span></span></span></b><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">The findings underscore the alarming rise in antibiotic resistance among <em>Acinetobacter</em> strains and highlight the necessity of implementing targeted antibiotic stewardship programs and localized surveillance systems to optimize treatment outcomes and curb the spread of resistance.</span></span></span></span></span></span></div> <br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><span style="font-size:12.0pt"><span style="background:white"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span></span> Acinetobacter spp, ICU, nosocomial infections, antibiotic-resistant pattern, Phoenix system 372 380 http://jabs.fums.ac.ir/browse.php?a_code=A-10-3493-1&slc_lang=en&sid=1 Tahmineh Ghanei Yazdi تهمینه قانعی یزدی Tahminehghanei@gmail.com 100319475328460030240 100319475328460030240 No Department of Biology, Kavian Institute of Higher Education, Mashhad, Iran گروه زیست‌شناسی، موسسه آموزش عالی کاویان، مشهد، ایران Nazanin Ataee نازنین عطایی ataee1357@yahoo.com 100319475328460030241 100319475328460030241 No Department of Biology, Kavian Institute of Higher Education, Mashhad, Iran گروه زیست‌شناسی، موسسه آموزش عالی کاویان، مشهد، ایران Parastoo Tajzadeh پرستو تاج زاده p.tajzadeh@gmail.com 100319475328460030242 100319475328460030242 Yes Department of Medical Laboratory Sciences, Kashmar Faculty of Medical Sciences, Mashhad University of Medical Sciences (MUMS), Mashhad, Iran گروه علوم آزمایشگاهی، دانشکده علوم پزشکی کاشمر، دانشگاه علوم پزشکی مشهد، مشهد، ایران Masoud Chaboksavar مسعود چابکسوار chaboksavar@kavian.ac.ir 100319475328460030243 100319475328460030243 No Kavian Institute of Higher Education, Mashhad, Iran موسسه آموزش عالی کاویان، مشهد، ایران